Objective To analyze the correlation between Peroxiredoxin 1(PRX1)and Calcium channelα2δ1 subunit(CACNA2D1)expression and the efficacy of intensity-modulated radiotherapy in patients with non-small cell lung cancer(NSCLC).Methods The cancer tissues in totally 112 NSCLC patients who re-ceived intensity-modulated radiotherapy in Shaanxi Cancer Hospital from January 2020 to January 2022 were selected as the NSCLC group,and according to the efficacy of radiotherapy,112 NSCLC patients were divided into an effective group(74 cases)and an ineffective group(38 cases).Normal lung tissues adjacent to cancer in 60 NSCLC patients undergoing surgical treatment were selected as the control group.Immunohistochemis-try and Western blot were used to detect the expression of PRX1 and CACNA2D1 in tissues.Spearman rank correlation analysis was used to investigate the correlation between PRX1 and CACNA2D1 expression.Multi-variate Logistic regression was used to analyze the influencing factors of efficacy of intensity-modulated radio-therapy in NSCLC patients.Results The positive rates of PRX1 and CACNA2D1 in NSCLC group were high-er than those in control group(x2=46.615,46.456,P＜0.05).The relative grayscale values of PRX1 and CACNA2D1 in NSCLC group were higher than those in control group(t=9.357,9.509,P＜0.05).The ex-pression of PRX1 and CACNA2D1 in NSCLC cancer tissues was positively correlated(r=0.724,P＜0.001).The positivity rates of PRX1 and CACNA2D1 in cancer tissues of patients with TNM stage Ⅲ-Ⅳ,low differ-entiation,and lymph node metastasis were higher than those of patients with TNM stage Ⅰ-Ⅱ,medium and high differentiation,and no lymph node metastasis(P＜0.05).The proportion of patients with TNM stageⅢ-Ⅳ,the proportion of patients with lymph node metastasis,positivity rate of PRX1 and positivity rate of CACNA2D1 in the ineffective group were higher than those in the effective group(P＜0.05).TNM staging stages Ⅲ-Ⅳ,lymph node metastasis,PRX1 positive,and CACNA2D1 positive were risk factors affecting the efficacy of intensity modulated radiotherapy in NSCLC patients(P＜0.05).Conclusion The positivity rates of PRX1 and CACNA2D1 in NSCLC patients are increased,which is related to TNM staging,differentiation de-gree and lymph node metastasis,and they are risk factors affecting the efficacy of intensity-modulated radio-therapy in NSCLC patients.