Objective: To investigate the vector control and prevention capability construction in county-level disease prevention and control institutions (CDCs) of Chongqing Municipality, so as to provide insights into the enhancement of vector control and prevention capability. Methods: Data on the establishment of vector control and prevention departments, staffing, laboratory construction, and self-evaluation of performance capability of 39 county-level CDCs in Chongqing Municipality were collected through questionnaire surveys in 2020 and 2023. The capability and changes of vector control and prevention in these CDCs were analyzed using descriptive methods. Results: Compare to 2020, the proportion of specialized vector control and prevention departments in county-level CDCs of Chongqing Municipality in 2023 increased from 10.26% to 17.95%. The number of staff engaged in vector control and prevention increased from 147 to 178. The proportions of full-time staff, permanent staff, and staff with relevant majors increased from 8.84%, 87.76% and 58.50% to 14.61%, 90.45% and 60.67%, respectively. The average laboratory areas increased from 14.49 m2 to 49.32 m2. The coverage rates of the laboratories for classification, identification and specimen storage and the laboratories for resistance determination increased from 20.51% to 61.54% and 43.59%. The coverage rates of the laboratories for the efficacy test of hygienic insecticides, the laboratories for the efficacy test of rodenticides in rooms, and the laboratories for etiology increased from 0 to 15.38%, 15.38% and 20.51%, respectively (all P<0.05). All county-level CDCs had the capabilities of population survey and density monitoring. The proportions of those with the capabilities of organizing prevention and control training, evaluating the effectiveness of vector control and prevention, and detecting pathogens carried by vectors increased from 46.15%, 30.77% and 0 to 69.23%, 53.85% and 38.46%, respectively (all P<0.05). Conclusions The set up of professional departments for vector control and prevention, the number of staff, the laboratory coverage rate, and the proportion of those with the performance capabilty in county-level CDCs in Chongqing Municipality were improved. However, it is necessary to strengthen the construction of the professional teams for vector control and prevention, and fully realize the laboratory function.

Objective　To analyze the epidemiological characteristics and vector emergency surveillance of dengue fever in Chongqing in 2023, by collecting relevant information. The aim is to provide scientific evidence for effective prevention and control measures in the future. Methods　The data on all dengue cases registered in Chongqing in 2023 were extracted from the Infectious Disease Surveillance System of China Information System Disease Control and Prevention. Descriptive epidemiological methods were used to analyze the epidemiological characteristics of dengue fever. The Breteau Index (BI) and double-layer mosquito net trap methods were adopted for the emergency surveillance of vector Aedes to evaluate the effect of vector Aedes emergency control. Statistical analysis was performed using SPSS 25.0 software. Results　A total of 119 cases of dengue fever were reported in Chongqing in 2023,including 92 imported cases and 27 local cases. The main sources of imported cases were Southeast Asia for foreign sources and Yunnan Province for domestic sources. Among the local outbreaks, Hechuan districts and Jiangbei districts had clustered outbreaks, with 15 cases (55.56%) and 5 cases (18.52%) respectively.Most cases occurred from August to October, with September being the peak period for imported and local cases. The majority of infected individuals were mainly inhousework and unemployed. The serotypes of dengue virus of local cases were mainly type Ⅰand type Ⅱ. After the local epidemic of dengue fever, 26 epidemic core areas were formed after integration. The average BI and net trap index of the first emergency surveillance in the core area were 16.51 and 4.72 mosquitoes per net per hour, respectively. The qualification rate of BI and net trap index in early emergency monitoring of Aedes mosquitoes were relatively low, at 7.69% and 30.77% respectively. By the 14th day, both qualification rates exceeded 95%. Conclusions　The prevention and control of dengue fever in Chongqing should focus on the domestic and international population flow. During the period from August to October, health quarantine efforts should be strengthened for individuals coming from or returning from Southeast Asian countries and Yunnan Province. Additionally, the monitoring and early warning of surveillance and early warning of vector Aedes, the control of breeding grounds, and adult mosquito eradication efforts should be further intensified to control the occurrence and spread of dengue fever effectively.

OBJECTIVE: To construct a HIV-1 gp120 transgenic mice (gp120 Tg) with vimentin (VIM) gene knockout. METHODS: Female HIV-1 gp120 Tg mice were mated to VIM heterozygote mice (F0). All the offspring mice were derived from these original founders so that both genotypes had the same mixed genetic background. The F1 mice were bred to generate of VIM, VIM, VIM/gp120 Tg and VIM/gp120 Tg mice. PCR was performed for genotyping of the mice, and the expressions of VIM and gp120 in the brain tissues were examined using immunoblotting. RESULTS: The results of PCR showed the presence of the target bands in VIM, VIM, VIM/gp120 Tg and VIM/gp120 Tg mice. In VIM/gp120 Tg mice, gp120 expression was detected throughout the brain regions while no VIM expression was detected. CONCLUSIONS We generated gp120 transgenic mouse models with VIM gene knockout, which facilitate the exploration of the role of VIM in gp120-induced neurotoxicity.
Animals ; Brain ; Disease Models, Animal ; Female ; HIV Envelope Protein gp120 ; HIV-1 ; Mice ; Mice, Knockout ; Mice, Transgenic ; Vimentin