Objective To explore the mechanism of Juanxiao decoction in regulating type 3 innate lymphoid cells(ILC3s)in treating obese asthma.Methods Sixty male BALB/c mice were randomly divided into a normal group,model group(high-fat diet+OVA),Juanxiao decoction groups(low,middle,and high doses of 8.5,17,and 34 g/kg,respectively),and dexamethasone group(1 mg/kg)with 10 mice in each group.Except for the normal group,the other groups were fed a high-fat diet for 12 weeks,and OVA sensitization by inhalation of an atomized OVA solution was used to establish the obese asthma model.From the first inhalation,the low-,medium-,and high-dose groups of Juanxiao decoction and the dexamethasone group were administered corresponding drugs by gavage,whereas normal and model groups were administered equal amounts of saline by gavage for 7 days.The state of mice and changes in typical symptoms of obese asthma were observed.At 24 hours after the last challenge,a fully automated biochemical analyzer was used to assess four blood lipids and count inflammatory cells in alveolar lavage fluid(BALF).Hematoxylin-eosin staining was used to observe morphological changes in lung tissue and abdominal fat.Enzyme-linked immunosorbent assays were used to measure the immunoglobulin E in BALF and serum,and interleukin(IL)-1β,IL-13,and mouse thymus activation regulating chemokine(CCL17)in lung tissue.IL-17A+ILC3 and IL-22+ILC3 in lung tissue and peripheral blood were analyzed by flow cytometry.Western blot was used to detect expression of P-STAT3 protein in lung tissue.Results Compared with the normal group,model group mice showed infiltration of airway inflammatory cells and thickening of airway walls.However,compared with the model group,lung inflammation in dexamethasone and Juanxiao decoction groups was improved,especially in middle-and high-dose groups.Compared with the normal group,IL-1β,IL-17A+ILC3,IL-13,and CCL17 in lung tissue of the model group were significantly increased(P＜0.05),whereas the proportion of IL-22+ILC3 and expression of P-STAT3 were significantly decreased(P＜0.01,P＜0.05).Compared with the model group,IL-1β,IL-17A+ILC3,IL-13,and CCL17 in lung tissue were significantly decreased and the proportion of IL-22+ILC3 and expression of P-STAT3 were significantly increased in middle-and high-dose Juanxiao decoction groups(P＜0.05,P＜0.01,P＜0.001).Conclusions Juanxiao decoction improves the inflammatory environment of obese asthmatic mice and alleviates lung inflammatory and allergic reactions.Its mechanism may be related to regulating secretion of cytokines by ILC3s.

Objective To analyze the relevant risk factors for Chinese adult urinary lithiasis.Methods We searched the PubMed,Embase,Cochrane Library databases,the Chinese Biomedical Literature Database,VIP Database,China Knowledge Network (CNKI) database,Wanfang database by computer.The search was set for all case-control studies on urinary lithiasis with Chinese patients.Two authors independently screened the literature,extracted literature data,and evaluated the quality of the literature.The software Revman 5.3 was used to perform meta-analysis in this study.Results A total of 25 studies in this study were case-control studies,with a total of 8 524 cases of urinary lithiasis and 42 239 cases of non-urinary lithiasis.A total of 9 risk factors of urinary lithiasis were screened:low intake of water [OR =2.64(95％CI 2.00-3.48),P ＜0.001],overweight [OR =2.36(95％ CI 1.31-4.23),P =0.004],tobacco consumption[OR =2.10(95％ CI 1.26-3.48),P =0.004],high salt diet [OR =1.88 (95％ CI 1.21-2.91),P =0.005],alcohol consumption [OR =1.85 (95％ CI 1.57-2.17),P ＜ 0.001],low vegetable consumption [OR =1.64 (95 ％ CI 1.05-2.54),P =0.03]、high protein diet [OR =1.49(95％ CI 1.23-1.82),P ＜0.001],low fruit consumption [OR =1.39(95％ CI 1.26-1.53),P ＜0.001] and tea consumption [OR=1.23(95％CI1.10-1.37),P＜0.001].Conclusions Lowintake of water,overweight,tobacco consumption,high salt diet,alcohol consumption,low vegetable consumption,high protein diet,low fruit consumption and tea consumption could be the risk factors for chinese adult urinary lithiasis.

Inhibitory ability of children with developmental dyscalculia (DD) was investigated to explore the cognitive mechanism underlying DD.According to the definition of developmental dyscalculia,19 children with DD-only and 10 children with DD&RD (DD combined with reading disability) were selected step by step,children in two control groups were matched with children in case groups by gender and age,and the match ratio was 1∶1.Psychological testing software named DMDX was used to measure inhibitory ability of the subjects.The differences of reaction time in number Stroop tasks and differences of accuracy in incongruent condition of color-word Stroop tasks and object inhibition tasks between DD-only children and their controls reached significant levels (P＜0.05),and the differences of reaction time in number Stroop tasks between dyscalculic and normal children did not disappear after controlling the non-executive components.The difference of accuracy in color-word incongruent tasks between children with DD&RD and normal children reached significant levels (P＜0.05).Children with DD-only confronted with general inhibitory deficits,while children with DD&RD confronted with word inhibitory deficits only.

Inhibitory ability of children with developmental dyscalculia (DD) was investigated to explore the cognitive mechanism underlying DD. According to the definition of developmental dyscalculia, 19 children with DD-only and 10 children with DD&RD (DD combined with reading disability) were selected step by step, children in two control groups were matched with children in case groups by gender and age, and the match ratio was 1:1. Psychological testing software named DMDX was used to measure inhibitory ability of the subjects. The differences of reaction time in number Stroop tasks and differences of accuracy in incongruent condition of color-word Stroop tasks and object inhibition tasks between DD-only children and their controls reached significant levels (P<0.05), and the differences of reaction time in number Stroop tasks between dyscalculic and normal children did not disappear after controlling the non-executive components. The difference of accuracy in color-word incongruent tasks between children with DD&RD and normal children reached significant levels (P<0.05). Children with DD-only confronted with general inhibitory deficits, while children with DD&RD confronted with word inhibitory deficits only.