Objective To investigate the relationship between collagen type Ⅰ alpha 1 chain(COL1A1)and mucin 4(MUC4)expression and clinicopathological features and prognosis in patients with nasopharynge-al carcinoma.Methods A total of 269 suspected patients with nasopharyngeal carcinoma admitted to a hospi-tal from December 2020 to June 2021 were selected as the study subjects,123 patients with nasopharyngeal carcinoma confirmed by pathological examination were selected as the study group,and 146 patients with na-sopharyngeal carcinoma ruled out by pathological examination were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression of COL1A1 and MUC4,and immunohistochemical staining was used to detect the protein expression of COL1A1 and MUC4.Survival curves were drawn by Kaplan-Meier method to analyze the relationship between COL1A1 and MUC4 protein expression and survival of nasopharyngeal carcinoma patients.COX regression analysis of nasopharyn-geal carcinoma patients with adverse prognostic factors.Receiver operating characteristic(ROC)curve was drawn to analyze the value of COL1A1 and MUC4 mRNA expression in predicting poor prognosis of patients with nasopharyngeal carcinoma.Results Compared with the control group,the mRNA expressions of COL1A1 and MUC4 in the study group were increased,and the difference was statistically significant(P＜0.05).The positive expression rate of COL1A1 protein in the study group was 58.54％(72/123)higher than that in the control group[41.10％(60/146)],and the difference was statistically significant(P＜0.05).The positive expression rate of MUC4 protein was 63.41％(78/123)higher than that of control group[33.56％(49/146)],and the difference was statistically significant(P＜0.05).The expressions of COL1A1 and MUC4 protein were correlated with TNM stage,depth of tumor invasion and lymph node metastasis(P＜0.05).Compared with the patients with good prognosis,the expressions of COL1A1 and MUC4 mRNA were in-creased in the patients with poor prognosis,and the differences were statistically significant(P＜0.05).The 3-year cumulative survival rate of patients with positive expression of COL1A1 protein was significantly lower than that of patients with negative expression of COL1A1 protein,and the difference was statistically signifi-cant(P＜0.05).The 3-year cumulative survival rate of patients with positive expression of MUC4 protein was significantly lower than that of patients with negative expression of MUC4 protein,and the difference was sta-tistically significant(P＜0.05).TNM stage Ⅲ/Ⅳ,tumor invasion stage T3/T4,lymph node metastasis,posi-tive expression of COL1A1 and MUC4 protein were risk factors for poor prognosis(P＜0.05).ROC curve a-nalysis results showed that the area under the curve(AUC)of COL1A1 and MUC4 mRNA combined to pre-dict poor prognosis of nasopharyngeal carcinoma patients was significantly greater than the AUC of MUC4 mRNA alone,and the difference was statistically significant(Z=2.248,P=0.025).Conclusion The expres-sion of COL1A1 and MUC4 in patients with nasopharyngeal carcinoma is increased,which is closely related to the clinicopathological features and prognosis of patients with nasopharyngeal carcinoma,and has important reference value for the prognosis evaluation of patients with nasopharyngeal carcinoma.

ECG signals and sleep monitoring parameters complement each other and can be used for qualitative diagnosis of sleep apnea syndrome and cardio-related diseases.However,due to the limitations of the instrument volume and the detection environment,it is often challenging to integrate these two functions in practical applications.In this paper,a 12-lead dynamic electrocardiograph integrated with sleep monitoring is designed.The system's volume is reduced by combining the integrated ECG simulation front end with a miniature sensor.The system achieves the extraction,conditioning,and calculation of 12-lead ECG signals and sleep-related parameters and writes the data to a memory card in real time,which offers convenience for users and doctors in the diagnostic process.

Cardiopulmonary exercise testing(CPET)refers to a method of measuring various indicators of the human body under gradually increasing exercise loads to objectively evaluate cardiopulmonary reserve function and exercise endurance.Currently,CPET detection systems primarily measure subjects'ECG,respiratory flow,oxygen(O2),and carbon dioxide(CO2)parameters.This paper introduces a non-invasive multi-parameter exercise cardiopulmonary function evaluation system that incorporates impedance cardiography monitoring.The system integrates impedance cardiography monitoring with conventional CPET detection parameters and detects changes in the hemodynamic parameters of the body during exercise,aiding in the evaluation of exercise capacity.Additionally,the system features a portable design with Wi-Fi wireless transmission,which enhances its applicability.

This study was designed to evaluate the correlation between immune antibodies and pelvic inflammatory disease(PID)using retrospective analysis.Cases were selected from 171 patients who met the diagnosis of PID in Liuzhou People's Hospital of Guangxi Province from January 2022 to March 2023,and the PID patients were further divided into simple PID group(53 cases)and in PID combined with reproductive tract infection group(118 cases)according to the presence or absence of reproductive tract infections,while 83 cases of women who did not meet the specific diagnostic criteria of PID and did not have reproductive tract infections were selected as the control group during the same period.The positive rate of immune antibodies in the three groups were observed and compared to explore the relationship between immune antibodies and PID.Data showed that the positive rates of immune antibodies were significantly higher in the PID alone group and the PID combined with reproductive tract infection group than that in the control group.Furthermore,the positive rate of immune antibody TPOAb was significant difference in the PID combined with reproductive tract infection group and the PID alone group(P<0.05).In conclusion,TPOAb is closely associated with reproductive tract infections.

Objective To prepare Zishen pills as gel plaster according to the prescription,and investigate its transdermal absorption characteristics in vitro.Methods Based on preliminary experiments,the matrix prescription of the gel plaster was optimized by single-factor tests and the Box-Behnken design.Evaluation indicators included initial viscosity,viscosity retention and sensory scores.The modified Franz diffusion cell was used to investigate the effect of penetration enhancers on the transdermal characteristics of gel plaster in vitro,with the permeability of neomangiferin,phellodendrine hydrochloride,mangiferin and berberine hydrochloride as evaluation indicators.Results The prescription dosage of the preferred matrix for the Zishen gel plaster was sodium polyacrylate NP700 2.55 g,glycerin 11.04 g,polyvinylpyrrolidone K90 1.13 g,tartaric acid 0.1 g,glycyrrhizin 0.1 g,kaolin 0.3 g,and distilled water 15 g.Among different types and concentrations of permeation enhancers,5%aminoketone showed the best permeation performance.The permeation rates for neomangiferin,phellodendrine hydrochloride,mangiferin,and berberine hydrochloride were 1.5338,1.7809,2.3247 and 20.0899 μg·(cm2)-1·h-1,and the penetration rates were 2.4319,1.9408,1.9604 and 1.4701,respectively.The percutaneous absorption curve of the drug conformed to the zero-order kinetic equation.Conclusion The preparation process of the obtained gel plaste is stable and feasible,with good adhesive properties,sustained drug release,and favorable in vitro percutaneous permeability,indicating potential clinical application value.

Ischemic-type biliary lesion (ITBL) refers to biliary tract injury caused by insufficient blood supply of hepatic artery, which is one of the main factors affecting the long-term survival and quality of life of liver transplant recipients. The incidence of ITBL is associated with cold and warm ischemia, acute and chronic rejection, cytomegalovirus infection and the bile effect, etc. The occurrence of ITBL is a complicated process involving with multiple factors and steps. The therapeutic option of ITBL is extremely limited. A large proportion of ITBL patients should undergo repeated liver transplantation. ITBL has become one of the most critical factors preventing further advancement of liver transplantation. Hence, it is of significance to strengthen prevention and explore more effective modalities. Recent studies have found that toxic injury of bile salts plays a central role in ITBL. Active regulation of bile components, regulation of bile acid-related receptor expression and blockage or activation of bile acid-related signaling pathways probably have potentials in the prevention and treatment of ITBL. In this article, the cytotoxicity of bile salts and the mechanism of bicarbonate umbrella in the incidence and progression of ITBL after liver transplantation were reviewed, aiming to provide reference for the diagnosis and treatment of ITBL.

HMG-CoA reductase (HMGCR) protein is usually upregulated after statin (HMGCR inhibitor) treatment, which inevitably diminishes its therapeutic efficacy, provoking the need for higher doses associated with adverse effects. The proteolysis targeting chimera (PROTAC) technology has recently emerged as a powerful approach for inducing protein degradation. Nonetheless, due to their bifunctional nature, developing orally bioavailable PROTACs remains a great challenge. Herein, we identified a powerful HMGCR-targeted PROTAC (

Objective::To evaluate the intervention effect of Bushen Tongluo formula on zebrafish osteoporosis model and to clarify its regulation of autophagy mechanism on osteoclast differentiation. Method::The 260 young zebrafishes (3 dpf) were selected, the zebrafish osteoporosis model was established with 25 mmol·L^-1 prednisolone (Pred) for 3 days, confirming the successful model by calcein staining. The zebrafishes were divided into control group, Pred group (25 mmol·L^-1), etidronate disodium (ED) group (300 mg·L^-1), Bushen (BS) group (180 mg·L^-1), Tongluo (TL) group (30 mg·L^-1), and Bushen Tongluo (BSTL) group (210 mg·L^-1), 40 tails per group. After intervened with medicine for 4 days, the calcein staining was adopted to count the vertebral bone fluorescence area of zebrafish, Real-time PCR was adopted to detect the mRNA expression of akaline phosphatase (ALP), bone morphogenetic protein 2b (BMP-2b), runt-related protein 2 (Runx2) and cathepsin K (CTSK), phosphorus and tartrate-resistant acid phosphatase (TRAP), nuclear factor of activated T-cells, cytoplasmic-1 (NFATC-1). Divided RAW264.7 cells into control, Rankl induction (10 μg·L^-1), BS (180 mg·L^-1), TL (30 mg·L^-1), and BSTL group (210 mg·L^-1) after they were cultured to 80%-90% density. The expression of actin ring was detected by phalloidin cytoskeleton staining. The mRNA expression of TRAP, CTSK and autophagy-related genes 5 (ATG5), autophagy-related genes 7 (ATG7), and ubiquitin-binding protein p62 (p62) were detected by Real-time PCR. Result::Compared with the control group in vivo, the vertebral area and ALP, BMP-2b, and Runx2 expression of zebrafish in the Pred group were significantly decreased (P<0.01), and CTSK, TRAP, and NFATC-1 expression of zebrafish were significantly increased (P<0.01). Compared with the Pred group, the vertebral area of the TL (P<0.05) and BSTL group (P<0.01) increased significantly. The expressions of ALP, BMP-2b, and Runx2 in the BS, TL, and BSTL group were significant increased (P<0.01). The expression of CTSK, TRAP, and NFATC-1 in BSTL group were significant decreased (P<0.05, P<0.01). Compared with the control group in vitro, the number of actin rings (P<0.01) and CTSK, TRAP, ATG5 (P<0.01) expression and ATG7, p62 (P<0.01) expression were significantly increased in the Rankl-induced group. Compared with the Rankl induction group, the number of actin rings and CTSK and TRAP expression were significantly decreased in the BS, TL, and BSTL group (P<0.05, P<0.01), while the expression of ATG5, ATG7, and p62 were significant decreased in the BSTL group (P<0.05). Conclusion::BSTL can significantly improve prednisolone-induced zebrafish osteoporosis and inhibit osteoclast differentiation by reducing autophagy-related gene expression.