Objective To analyze the correlation between white blood cell(WBC)and blood cell indexes in megaloblastic anemia(MA)patients.Methods A total of 79 patients diagnosed as MA by bone marrow cell morphology at Adicon Medical Laboratory Center from June 2023 to June 2024 were included in MA group,and another 40 healthy physical examination subjects during the same period were included in health group.The levels of WBC,red blood cell(RBC),platelet(PLT),hemoglobin(Hb)and mean corpuscular hemoglobin(MCH),mean corpuscular volume(MCV)were measured and compared between two groups.Spearman correlation was used to analyze the correlation of each parameter.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of WBC in MA.Results The levels of WBC,PLT,Hb and RBC in MA group were significantly lower than those in healthy group(P＜0.05),MCH and MCV levels were significantly higher than those of healthy group(P＜0.05).WBC was positively correlated with PLT,Hb and RBC(P＜0.05),and were negatively correlated with MCH and MCV(P＜0.05).ROC curve analysis showed that the area under the curve of WBC diagnosis MA was 0.926(95％CI:0.876-0.975).The levels of PLT,Hb and RBC in severe and above group were significantly lower than those in mild to moderate group(P＜0.05).The degree of anemia in MA patients was negatively correlated with the levels of PLT,Hb and RBC(P＜0.05),and the more severe the anemia,the lower the levels of PLT,Hb and RBC.Conclusion The changes of WBC and RBC are related to the occurrence of MA,and WBC can be used as a reference index for the diagnosis of MA.

Objective To analyze the correlation between white blood cell(WBC)and blood cell indexes in megaloblastic anemia(MA)patients.Methods A total of 79 patients diagnosed as MA by bone marrow cell morphology at Adicon Medical Laboratory Center from June 2023 to June 2024 were included in MA group,and another 40 healthy physical examination subjects during the same period were included in health group.The levels of WBC,red blood cell(RBC),platelet(PLT),hemoglobin(Hb)and mean corpuscular hemoglobin(MCH),mean corpuscular volume(MCV)were measured and compared between two groups.Spearman correlation was used to analyze the correlation of each parameter.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of WBC in MA.Results The levels of WBC,PLT,Hb and RBC in MA group were significantly lower than those in healthy group(P＜0.05),MCH and MCV levels were significantly higher than those of healthy group(P＜0.05).WBC was positively correlated with PLT,Hb and RBC(P＜0.05),and were negatively correlated with MCH and MCV(P＜0.05).ROC curve analysis showed that the area under the curve of WBC diagnosis MA was 0.926(95％CI:0.876-0.975).The levels of PLT,Hb and RBC in severe and above group were significantly lower than those in mild to moderate group(P＜0.05).The degree of anemia in MA patients was negatively correlated with the levels of PLT,Hb and RBC(P＜0.05),and the more severe the anemia,the lower the levels of PLT,Hb and RBC.Conclusion The changes of WBC and RBC are related to the occurrence of MA,and WBC can be used as a reference index for the diagnosis of MA.

Objective:To investigate the impacts of ionizing radiation on protein expression profiles in esophageal tissues of rats using quantitative proteomics, in order to reveal the molecular mechanisms underlying the onset and development of radiation-induced esophagitis (RIE).Methods:A total of twenty-four male SD rats were divided by simple randomization into three groups: the control, 25 Gy irradiation, and 35 Gy irradiation groups, and their esophageal tissues were collected at 7 d post-irradiation to extract total protein. Then, changes in the protein expression profiles of the esophageal tissues in irradiated rats were investigated using tandem mass tag (TMT)-labeled quantitative proteomics and bioinformatics analysis. Additionally, the expressions of two key proteins, Hp and Ndufs4, were validated using immunohistochemistry and Western blot.Results:A comparison with the control group revealed a total of 847 differentially expressed proteins (DEPs; 483 up-regulated and 364 down-regulated) following 25 Gy irradiation and 699 DEPs (443 up-regulated and 256 down-regulated) following 35 Gy irradiation. Different radiation doses led to common 326 up-regulated proteins, which were mainly involved in biological processes and signaling pathways related to immune and inflammatory responses, and 210 down-regulated proteins, which were primarily involved in biological processes and signaling pathways related to energy production and metabolism. Furthermore, a total of 155 proteins were screened using a constructed protein protein interaction(PPI) network. Of these proteins, the up-regulated ones were most associated with three functional pathways, namely innate immune responses, complement and coagulation cascades, and innate immune system, while the down-regulated ones were most associated with energy acquisition via oxidizing organic compounds, oxidative phosphorylation, and the tricarboxylic acid (TCA) cycle and respiratory electron transfer. These functions were enriched with nine complement-related up-regulated and five mitochondria-related down-regulated proteins, respectively. Ionizing radiation significantly up-regulated Hp ( t = 27.94, 10.96, P<0.001) and down-regulated Ndufs4 ( t = 59.27, 54.07, P<0.001), consistent with the protein sequencing result. Conclusions:Ionizing radiation can change the protein expression profiles in the esophageal tissues of rats, and these DEPs are involved in multiple radiobiology-related functional pathways such as immune processes, inflammatory responses, and abnormal energy metabolism. Screening and validation of key proteins are helpful for identifying potential biomarkers of radiation-induced esophagitis.