Objective:To investigate whether the highly expressed chemokine ligand 17(CCL17)in cervical cancer(CC)pro-motes the progression of CC through M2 macrophage polarization.Methods:The effect of CCL17 on the expression of IL-27 in CC cell line was detected by flow cytometry and ELISA.The effects of CCL17 and IL-27 on proliferation and differentiation of U937 cells were analyzed.The co-culture model of macrophages and CC cells were established to detect the effects of CCL17 on the proliferation of U937 and CC cells.Results:The CC cells secreted significantly higher levels of IL-27 after stimulation with CCL17.In addition,IL-27 but not CCL17 significantly upregulated interleukin 27 receptor(IL-27R,consisted of WSX-1 and GP130)on U937 cells.IL-27 de-rived from CC cells can promote the proliferation of U937 cells and induce the polarization of M2 macrophages.In the co-culture sys-tem,the level of IL-27 secreted by CC cells stimulated by CCL17 was significantly increased,and both CCL17 and IL-27 promoted the proliferation of CC and U937 cells,and the effect of CCL17 was partially reversed by αIL-27.Conclusion:CCL17 promotes the proliferation of macrophages and induces M2 polarization by stimulating CC cells to secrete IL-27,thereby enhancing the crosstalk be-tween CC cells and macrophages.Eventually,CCL17 participates in the malignant evolution of CC.

Objective:To investigate whether the highly expressed chemokine ligand 17(CCL17)in cervical cancer(CC)pro-motes the progression of CC through M2 macrophage polarization.Methods:The effect of CCL17 on the expression of IL-27 in CC cell line was detected by flow cytometry and ELISA.The effects of CCL17 and IL-27 on proliferation and differentiation of U937 cells were analyzed.The co-culture model of macrophages and CC cells were established to detect the effects of CCL17 on the proliferation of U937 and CC cells.Results:The CC cells secreted significantly higher levels of IL-27 after stimulation with CCL17.In addition,IL-27 but not CCL17 significantly upregulated interleukin 27 receptor(IL-27R,consisted of WSX-1 and GP130)on U937 cells.IL-27 de-rived from CC cells can promote the proliferation of U937 cells and induce the polarization of M2 macrophages.In the co-culture sys-tem,the level of IL-27 secreted by CC cells stimulated by CCL17 was significantly increased,and both CCL17 and IL-27 promoted the proliferation of CC and U937 cells,and the effect of CCL17 was partially reversed by αIL-27.Conclusion:CCL17 promotes the proliferation of macrophages and induces M2 polarization by stimulating CC cells to secrete IL-27,thereby enhancing the crosstalk be-tween CC cells and macrophages.Eventually,CCL17 participates in the malignant evolution of CC.

Pancreatic cancer is a commonly malignant gastrointestinal tumor with an significantly increasing incidence.Those patients without nonspecific symptoms at early stage had mostly lost the opportunity of surgical therapy when pancreatic cancer was detected at advanced stage,and its prognosis is poor.Therefore,it is rather important to improve the early diagnosis of pancreatic cancer.In recent years,proteomics is developing rapidly.Proteomics technologies have been widely used in clinical research.Using proteomics technology screening pancreatic cancer tumor markers becomes the research focus,thus we try to find a kind of or a group of pancreatic tumor markers,so as to improve the diagnosis of pancreatic cancer.

ObjectiveTo find out the inhibitory effects of CD4 - CD8 - DNT cells on growth of which depresses the pancreatic cancer in vitro and in vivo.Methods The inhibitory effects of DNT cells on the growth of Panc- 1 were studied in vitro by MTT method.Eighteen BALB/c mice were divided into 3 groups randomly.Human pancreatic cancer xenografts were established in 2 groups randomly.The last group was injected the cell suspension which comprises DNT and Panc- 1 cells ( Panc- 1∶ DNT =1∶ 5 ).When the diameter of tumor was about 5 mm,the first 2 group mice were further divided into 2 groups randomly.One was control,treated with distilled water.The other was treated with celebrex (4 mg/d).The size of the tumors was calculated every 2 weeks and tumor growth curve was depicted.At the end of the treatments,the mice were sacrific and the tumors were harvested.The tumor inhibition rate was calculated.Results( 1 ) MTT study showed that DNT cells produced a dose- dependent inhibition of Panc- 1 proliferation in vitro.(2) The growth of transplanted pancreatic cancer was down-regulated by treatment of DNT cells.ConclusionDNT cells can inhibit the growth of pancreatic cancer in vitro and in vivo.

ObjectiveTo summarize the clinical features and surgical outcomes in solid pseudopapillary tumor of pancreas.MethodsA retrospective clinical analysis was made on 18 cases of solid pseudopapillary tumor of pancreas confirmed by pathological diagnosis from Jan.2000 to Feb.2011.ResultsThe median age of these cases was 27.8 years,ranging from 15 to 46 years.Fifteen cases were female and 3 cases were male.The size of the tumor ranged from 4.0 cm to 15.0 cm,with an average size of 7.1 cm.Eleven of 18 tumors(61.1％ ) had a well-defined capsule,and 5 tumors (27.8％ ) extended beyond the pancreas.Nine of the 18 tumors (50.0％) had a cystic component,and calcification was observed in 3 tumors ( 16.7％ ).The frequency of microscopic venous invasion,lymphatic invasion,and nerve invasion was 16％ (3 of 18),0 and 0 respectively.No lymph node involvement or liver metastasis was observed.Distal pancreatectomy plus splenectomy was done in 5 patients,spleen- preserving distal pancreatectomy in 3,medial pancreatectomy in 1,subtotal stomach- preserving pancreatoduodenectomy in 1,enucleation in 9.Fifteen patients were still alive without recurrent disease or metastasis after a median follow-up of 48 months.Conclusions These results demonstrated that solid pseudopapillary tumor of pancreas occurs mainly in young women,patients with solid pseudopapillary tumor of the pancreas had a favorable outcome after surgical treatment,including enucleation.

ObjectiveA comparative proteomic method was used to analyse serum proteins between pancreatic cancer patients and control group,and to find a new protential specific marker.MethodsComparative analysis on the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients,10 chronic pancreatitis patients,10 benign tumor patients and 40 cancer-free controls was carried out by 2D differential gel electrophoresis,and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry.ResultsTwo differentially expressed proteins:transthyretin and apolipoprotein E were identified.Those proteins were highly expressed in pancreatic carcinoma group compared with normal control group,chronic pancreatitis group and benign tumor group.Conclusion2D differential gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry technology in screening specific serum biomarkers of pancreatic cancer has a well repeatability and stability.The identified protein transthyretin in this study may be as specific serum biomarkers of pancreatic carcinoma.