Acute carbon monoxide poisoning (ACMP) is one of the most common gas poisonings in the emergency department, with tens of thousands of people seeking medical attention for carbon monoxide (CO) poisoning each year. The severity of poisoning is dependent upon environmental and human factors, with hypoxia and oxidative stress being important mechanisms of cardiac toxicity induced by CO. Myocardial involvement is common in moderate to severe ACMP, including myocardial injury, myocardial infarction, arrhythmia, and sudden death, which are associated with a high risk of death. Ferroptosis is a cell death mechanism caused by iron-dependent lipid peroxidation (LPO), although ferroptosis has been shown to play a critical role in various cardiovascular diseases, the potential mechanism by which it contributes to ACMP-induced myocardial injury is unclear. This review discusses the established link between ferroptosis and cardiovascular disease and summarizes the potential role of ferroptosis in ACMP-induced myocardial injury and the detrimental effects of ACMP on the heart. Elucidating these mechanisms could guide the development of novel therapeutic strategies that target ferroptosis to mitigate ACMP-induced myocardial injury. This review aims to provide a theoretical foundation for future research on the potential use of ferroptosis as a therapeutic target for ACMP-induced myocardial injury.
Humans ; Carbon Monoxide Poisoning/complications* ; Ferroptosis ; Lipid Peroxidation ; Myocardium/pathology* ; Oxidative Stress

Objective:To explore the inhibitory effect of protocatechuic acid (PCA) on the growth of ovarian cancer cell line A2780 and its mechanism.Methods:A2780 cells were cultured in vitro and randomly divided into a control group and protocatechuic acid-treated groups with concentrations of 5 μM and 10 μM. Cell proliferation was detected using the MTT assay; Cell migration ability was assessed through wound healing experiments; Cell cycle and apoptosis were analyzed using flow cytometry; Protein expression of apoptosis-related proteins was examined using Western blot.Results:Compared with the control group, the absorbance of the protocatechuic acid groups (5 μM, 10 μM) decreased after 48 hours, and protocatechuic acid significantly inhibited cell proliferation ( P<0.05) . In the 5 μM protocatechuic acid treatment group, the proportion of cells in the G2/M phase increased to 25.26%, and in the 10 μM treatment group, the proportion of cells in the G2/M phase increased to 24.99%. Protocatechuic acid groups could arrest the cell cycle of the human ovarian cancer cell line A2780 at the G2/M phase. Protocatechuic acid groups could also promote the increase of early and late apoptosis rates of A2780 cells, leading to the upregulation of the expression levels of related apoptotic proteins CASPASE-3 and Bax, while the expression level of the anti-apoptotic protein Bcl2 was downregulated. Conclusions:Protocatechuic acid can inhibit proliferation and migration of A2780 ovarian cancer cells, arrest the cell cycle of A2780 cells, and promote apoptosis. The mechanism of action may be related to the upregulation of apoptosis-related proteins BAX and caspase3.

Objective:To explore the inhibitory effect of protocatechuic acid (PCA) on the growth of ovarian cancer cell line A2780 and its mechanism.Methods:A2780 cells were cultured in vitro and randomly divided into a control group and protocatechuic acid-treated groups with concentrations of 5 μM and 10 μM. Cell proliferation was detected using the MTT assay; Cell migration ability was assessed through wound healing experiments; Cell cycle and apoptosis were analyzed using flow cytometry; Protein expression of apoptosis-related proteins was examined using Western blot.Results:Compared with the control group, the absorbance of the protocatechuic acid groups (5 μM, 10 μM) decreased after 48 hours, and protocatechuic acid significantly inhibited cell proliferation ( P<0.05) . In the 5 μM protocatechuic acid treatment group, the proportion of cells in the G2/M phase increased to 25.26%, and in the 10 μM treatment group, the proportion of cells in the G2/M phase increased to 24.99%. Protocatechuic acid groups could arrest the cell cycle of the human ovarian cancer cell line A2780 at the G2/M phase. Protocatechuic acid groups could also promote the increase of early and late apoptosis rates of A2780 cells, leading to the upregulation of the expression levels of related apoptotic proteins CASPASE-3 and Bax, while the expression level of the anti-apoptotic protein Bcl2 was downregulated. Conclusions:Protocatechuic acid can inhibit proliferation and migration of A2780 ovarian cancer cells, arrest the cell cycle of A2780 cells, and promote apoptosis. The mechanism of action may be related to the upregulation of apoptosis-related proteins BAX and caspase3.

Objective To investigate the clinical characteristics and dosimetric parameters associated with acute hematologic toxicity (AHT) resulting from radiation-induced damage to hematopoietic organs in patients undergoing chemoradiotherapy for cervical cancer and to provide a reference for establishing dose constraints in relevant regions of interest (ROIs) and predicting adverse tissue reactions during the development of clinical treatment plans. Methods A retrospective analysis was conducted on 556 patients with cervical cancer who underwent chemoradiotherapy at our hospital. Univariate (χ² and t-test) and multivariate (binary logistic regression analyses) methods were employed to investigate the association of clinical factors and pelvic dose-volume parameters with grade ≥ 3 AHT in patients with cervical cancer. Clinical factors comprised patients’ age, clinical stage, pathologic stage, whether the patient had received chemotherapy in the radiotherapy cycle of interest, and dose-volume dosimetric parameters V_x and D_mean for pelvic bone marrow (BM) and femoral head (FH) structures. Results The incidence of AHT among the included cases was 30.4% (169/556). Chi-square analysis of the clinical factors revealed that whether the patient had received chemotherapy, patient’s age, and pathologic stage had a significant impact on AHT. Univariate analysis showed that the factors associated with AHT were mean dose, V₅, V₁₀, V₁₅, V₂₀, and V₂₅ of BM and FH; dosimetric parameters such as V₃₅ of FH had a significant impact on the development of AHT. Multivariate logistic regression analysis identified V₁₅ of pelvic BM as an independent risk factor for AHT (P=0.041), with a threshold value of 84.29% as determined by a receiver operating characteristic (ROC) curve. Conclusion Whether a patient had received chemotherapy in the radiotherapy cycle of interest, and patient’s age and pathologic stage can serve as predictors of AHT. V₁₅ of BM is an independent risk factor for AHT development. Therefore, when formulating a treatment plan, it is crucial to ensure that pelvic V₁₅ remains below 84.29% to effectively reduce the incidence of grade ≥ 3 acute bone marrow depression.

Objective:To investigate the performance of W2 plastic scintillator in megavolt photon and electron beams.Methods:The photon and electron beam energy provided by linear accelerator was used to collect data of the W2 scintillator. The parameters include the electrometer reading stability, W2 dose and dose rate linearity, and angular response. And the dose uncertainty of the W2 correction factors was also investigated.Results:The standard deviation of the electrometer reading stability was between 0.03 and 0.47. The linear regression factors of W2 dose were all 1.0; the maximum deviation of the dose rates was 0.61%. The Cerenkov light radiation correction factor(CLR) for 6 and 10 MV were 0.741 and 0.746, respectively, and the CLR for 6, 9, 12 and 15 MeV were 0.750, 0.753, 0.757 and 0.757, respectively. The maximum deviation of dose uncertainty for 15 MeV was 3.15%.Conclusions:The signal obtained by the blue and green channel was no angular dependence, the same as the high-energy electron beam, which verified that the Cerenkov radiation correction factor has good linearity. W2 plastic scintillator can be applied to non-coplanar radiotherapy dosimetry.

Aquatic plants and the epiphytic microorganisms are important contributors to the purification of constructed wetlands. Taking the dragon-shaped water system of Beijing Olympic Park as a model, this study analyzed the structure and function of the microbial communities reside the sediment, the water body and the rhizosphere and phyllosphere of three submerged plants-Vallisneria natans, Myriophyllum verticillatum, and Potamogeton pectinatus using high-throughput sequencing technology. The results showed that the microbial diversity from the highest to the lowest were samples from sediment, plant rhizosphere, plant phyllosphere and water. The microbial diversity of plant phyllosphere samples were significantly higher than those of the water body. LEfSe analysis showed that different habitats enriched different microbial groups. The sediments mainly enriched anaerobic microbes, while the water body and the phyllosphere of plants mainly enriched aerobic microbes, and the rhizosphere of plants had the both. Functional prediction analysis showed that the abundance of denitrification marker genes in phyllosphere samples was higher than that in samples from rhizosphere, sediment and water body, and the abundance of denitrification marker genes in phyllosphere samples of M. verticillatum and P. pectinatus was higher than that of V. natans. This study could serve as a guidance for the selection of submerged plants and functional microorganisms for constructed wetlands.
Beijing ; Hydrocharitaceae ; Microbiota ; Rhizosphere ; Water

Breast cancer is the malignant tumor with the highest incidence among women in the worldwide. The triple-negative breast cancer (TNBC) has the strongest immunogenicity. Because of the lack of clear molecular targets, TNBC is a subtype of breast cancer with more difficulties in the treatment and poorer prognosis compared to other breast cancer subtypes. Blocking the programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) signaling pathway has been a hot spot of research and treatment of tumors. PD-1/PD-L1 inhibitors provide new treatment options for TNBC. This article reviews the research progress of PD-1/PD-L1 inhibitors alone or in combination with other drugs in treatment of TNBC, intending to provide the theoretical basis for basic or clinical studies.

Objective To compare two pretreatment plan QA methods for HalcyonTM accelerator using Portal Dosimetry (PD),and PTW OCTAVIUS 1500 detector array + Octagonal phantom (Oct 1500)respectively.Methods Parallel measurement-based pretreatment QA using two methods was performed for 22 IMRT/VMAT plans (74 fields) that have been used to treat 20 patients recruited in the Halcyon clinical trial.Several γ 2D comparisons were also applied to provide guidelines for Halcyon planning QA.Results Using Oct1500 method,the γ 2D passing rates for 74 fields in 22 Plans were 95.26±3.59,95.01±3.62 (Local Dose),99.05± 1.35,98.57± 1.96 (Max Dose) respectively.Two-related samples non-parametric tests suggested that the differences between the evaluation criteria were of statistical significance (Z =-7.220,-4.108,P＜0.05).For PD method,the γ 2D passing rates were 84.11％ ± 1.35％ (1 mm/1％),99.07％± 1.35％ (2 mm/2％),and 99.86％ ± 1.35％ (3 mm/3％).Two-related samples non-parametric tests suggested that the differences between evaluation criteria of PD method were statistically significant (Z =-7.475,-7.475,-6.906,P＜0.05).For 74 fields and max dose,3 mm/3％ evaluation criteria,two-related samples non-parametric tests suggested that the differences between PD and Oct1500 method were statistically significant (Z=-5.072,P＜0.05).Conclusions Both methods can be used for Halcyon pretreatment plan QA.PD is superior to Oct1500 with respect to efficiency and spatial resolution-induced verification accuracy.The criteria of 2 mm/2％ for PD,and Max Dose/3 mm/3％ for Oct1500 was suggested.

Objective To evaluate the feasibility and dosimetric features of a volume modulated arc therapy (VMAT)-configured model in knowledge-based optimization of fixed-field dynamic intensitymodulated radiotherapy (IMRT) plans based on the Varian RapidPlan system.Methods ① A dose-volume histogram prediction model was trained with 81 qualified preoperative VMAT plans for rectal cancer and then statistically verified.② For clinically approved 10 IMRT plans with the same dose prescription,the above model was used to automatically generate new optimization parameters and dynamic muhileaf collimator (MLC) sequences with field geometry and beam energy unchanged.③ In order to rule out the disparities between different versions,a single algorithm was used to calculate the absolute doses of the original and new plans.④ Statistical analyses were performed on dosimetric parameters after comparable target dose coverage was achieved in the two plans by appropriate normalization.Results On the basis of similar target dose homogeneity and coverage,RapidPlan significantly reduced the doses to the urinary bladder (D50％ by 9.01 Gy,P =0.000;Dmean by 8.08 Gy,P =0.005) and the femoral head (D50％ by 4.20 Gy,P =0.000;Dmean by 3.84 Gy,P=0.005) but significantly elevated the mean total number of monitor units (1211±99 vs.771±79,P=0.000) and the number of fields with multiple MLC carriage groups.The knowledge-based semi-automated optimization caused a significantly larger number of high-dose hotspots but a similar D2％ (52.54 vs.52.71 Gy,P=0.239).Conclusions The VMAT model can be used for the knowledge-based semi-automated optimization of IMRT plans to enhance the efficiency and OAR protection.However,the resulting high-dose hotspots need further manual intervention.