Objective: To compare the difference in somatic gene mutation of PTC subtypes between 114 patients with papillary thyroid carcinoma (PTC) and The Cancer Genome Atlas (TCGA) database. Methods: Totally 114 PTC patients admitted to The First Affiliated Hospital of Nanjing Medical University were recruited. The 18 hotspot genes associated with thyroid cancer were detected in thyroidectomy specimens were using next generation sequencing. PTC data were downloaded from the TCGA database in the cBioPortal website, and the difference in the somatic gene mutation was compared between 114 PTC patients and the TCGA database Results: The 114 PTC patients included 73 women (64.04%) and had a mean age of (39.23±13.18) years. The prevalence of BRAF V600E (66.67% vs. 48.68%), TERTp (3.51% vs. 0.41%), PDGFRA (1.75% vs. 0%), PTEN (3.51% vs. 0.41%) and TP53 gene mutations (4.39% vs. 0.61%) was significantly higher among the 114 PCT patients than in the TCGA database (P<0.05). The prevalence of BRAF V600E (80.88% vs. 54.99%), TP53 (7.35% vs. 0.57%) and TSHR gene mutations (2.94% vs. 0%) was significantly higher in classical PTC(CPTC) patients than in the TCGA database, and the prevalence of BRAF V600E (36.84% vs.13.86%) and TERTp gene mutations (10.53% vs. 0%) was significantly higher in follicular variant PTC (FVPTC) patients than in the TCGA database. According to the American Thyroid Association Risk Stratification of Thyroid Cancer Recurrence, the prevalence of BRAF V600E and TP53 gene mutations was 77.14% and 8.57% among moderate-risk CPTC patients, the prevalence of BRAF V600E gene mutation was 27.27% among low-risk FVPTC patients, and the prevalence of TERTp gene mutation was 33.33% among moderate-risk FVPTC patients, which were all higher than in the TCGA database (55.10%, 0%, 3.28%, and 0%, respectively; P<0.05). Conclusion There are significant differences in the type and rate of somatic gene mutations between 114 PTC patients and the TCGA database.

In order to explore the effect of extract (SNE) on skin wound healing in mice and its mechanism, hemostasis effect of SNE was measured, the mouse skin wound model was established by full-thickness excision. The morphological changes of the wound were observed after the treatment with SNE and the healing rate was measured. The changes of wound histology were observed by hematoxylin eosin (HE) staining, Masson staining and transmission electron microscope (TEM). The expression of cell factors and related proteins was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that the SNE possessed hemostatic function. SNE could obviously improve the healing rate of wound in mouse and shorten time of scab removal compared with the none-treatment (NT) group ( < 0.05).The pathological histology analysis results showed complete epidermal regeneration, with remarkable capillary and collagen fiber observed in the SNE group. The expression level of tumor necrosis factor-α (TNF -α), interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in SNE group was significantly lower than that of the NT group on 7 d ( < 0.05). Moreover, compared with the NT group, the gene expressions level of Smad7 was significantly increased and the level of type II TGF-β receptors (TGF-βRII), collagen I (COL1A1) and α-smooth muscle actin (α-SMA) were significantly reduced in the SNE group on 28 d ( < 0.05), but the difference was not statistically significant compared to Yunnanbaiyao group (PC group) ( > 0.05). These results indicated that SNE possessed obvious activity of accelerating wound healing and inhibiting scar formation, and its mechanism was closely related to hemostatic function, regulation of inflammatory factors, collagen deposition, collagen fiber remodeling and intervening TGF-β/Smads signal pathway. Therefore, SNE may have promising clinical applications in skin wound repair and scar inhibition.

OBJECTIVE: To establish a set of evaluation system of digital radiography clinical value and provide foundation for the maturity assessment of digital radiography. METHODS: The evaluation system of clinical value of digital radiography was established by literature survey,expert consultation,and percentage weight method. RESULTS: The expert authority coefficients were 0.81 and 0.88,respectively.After two rounds of consultation,variation coefficients of each item ranged from 0 to 0.207,and the coefficient coordination were 0.599.The index system consisted of 5 first-level indexes and 12 second-level indexes.The weights of first-level indexes such as image quality,safety,usability,economic and social indicators share of the weight are 0.298,0.294,0.199,0.121 and 0.088 respectively. CONCLUSIONS A completed and scientific evaluation system was established,which provides a scientific assessment tool for clinical value of digital radiography.
Delphi Technique ; Radiographic Image Enhancement ; standards

Objective To evaluate the efficacy and safety of compound Kushen injection as an adjuvant for chemotherapy in treatment of advanced digestive tract cancer. Methods A prospective, randomized, parallel controlled, single center clinical trial was conducted. One hundred and forty-four patients with advanced gastrointestinal cancer admitted to the Department of Oncology of Fangta Traditional Chinese Medicine (TCM) Hospital of Songjiang District in Shanghai from July 2014 to December 2016 were enrolled, and they were divided into a control group and an observation group, 72 cases in each group. The patients in control group received routine chemotherapy, and on the basis of routine chemotherapy, the patients in the observation group was given compound Kushen injection 20 mL having diluted with 250 mL sodium chloride for intravenous drip, once a day for 14 days constituting 1 therapeutic course. After consecutive 2 therapeutic courses, the clinical efficacy was observed, the differences in disease control rate (DCR), Karnofsky score (KPS), TCM syndrome curative effect and adverse reactions were compared between the two groups, follow-up was carried out for 7-24 months, survival rate and progression free survival (PFS) time was observed in the two groups. Results After treatment, the DCR, KPS score, total effective rate of TCM syndrome in the observation group were significantly higher than those of control group [DCR: 59.7% (43/72) vs. 43.1% (31/72), KPS score: 70.9±6.2 vs. 64.8±4.8, total curative effect of TCM syndrome: 63.89% (46/72) vs. 41.67% (30/72), all P < 0.05], and the adverse effect rate was significantly lower than that of control group [25.0% (18/72) vs. 41.7% (30/72), P < 0.05]. In the end of follow-up, there were 12 survival cases in the control group, and 22 survival cases in observation group, and survival rate and the PFS time in observation group were significantly longer than those in the control group [survival rate: 30.6% vs. 16.7%; PFS time (months): 15.3±4.0 vs. 13.2±4.2, P < 0.05]. Conclusions The therapeutic effect of using Kushen injection as an adjuvant for chemotherapy in treatment of patients with advanced digestive tract cancer is relatively good, can improve the life quality of patients, and lower the incidence of adverse reactions.

Objective To observe the effects of compound Sophorae Flavescentis Radix (Kushen) Injection on immune function and quality of life in ovarian cancer patients with chemotherapy. Methods Sixty cases of ovarian cancer patients with chemotherapy were randomly divided into the observation group and the control group. Patients in the observation group were treated with paclitaxel plus carboplatin chemotherapy plus compoundKushen Injection for intravenous injection, while the patients in the control group were treated with paclitaxel plus carboplatin chemotherapy for three cycles. Changes in peripheral venous blood, the level of T cell subsets and NK cell, body weight, quality of life of 1 day before chemotherapy and 1 week after chemotherapy were observed.Results The mean values of CD3+, CD4+, CD4+/CD8+ and NK cells in the observation group improved to certain different degrees, with statistical significance compared with the control group (P<0.05). The body weight of observation group after chemotherapy increased than before chemotherapy (P<0.05);the control group had no significant difference (P>0.05). Karnofsky score of the observation group increased after chemotherapy (P<0.05);the control group had no obvious change (P>0.05).Conclusion CompoundKushen Injection can effectively enhance the immune function of ovarian cancer patients with chemotherapy, increase the weight of patients, and improve their quality of life.

OBJECTIVETo analyze the characteristics of autopsies in medical dispute.

METHODSThe data of 202 autopsy cases in medical disputes performed by the Department of Pathology of Jiangxi Medical College from January 2001 to December 2010 were retrospectively analyzed.

RESULTSThe number of autopsy in medical disputes increased year by year. Neonatal, infant and 30 ≊ 60y were more common age groups in dispute; the gender ratio of male to female was 2:1 and more cases were from hospitals in rural areas. Most medical disputes came from in departments of pediatric, obstetrics and gynecology and general surgery. Death caused by cardiovascular diseases ranked at the first place.

CONCLUSIONAutopsy is important in medical disputes to define the cause of death and to preserve evidence.

Adolescent ; Adult ; Aged ; Autopsy ; statistics & numerical data ; Cardiovascular Diseases ; mortality ; Cause of Death ; Child ; Child, Preschool ; Dissent and Disputes ; Female ; Hospital Departments ; statistics & numerical data ; Humans ; Infant ; Infant, Newborn ; Male ; Malpractice ; Middle Aged ; Retrospective Studies ; Sex Ratio ; Young Adult

Objective To study the internal linkage of Hetu and Luoshu that reveals the law from which the universe occurs and develops. Methods By finding out the root causing the difference of the Middle Palace Numbers between Hetu and Luoshu, we try to interpret the origin of the universe and its development. Results The number Five and Ten in the Middle Palace of Hetu are generated by the combination of Sheng-cheng Numbers in Hem's four directions which are One-six, Two-seven, Three-eight, Four-nine which can be producted by each other orderly. The number Five in the Middle Palace of Luoshu is generated by the Yang numbers One-Three-Seven-Nine in Luoshu's four positive directions which have properties of Duidai and Heshi and the Yin numbers of Two-Four-Six-Eight in Luoshu's four comers do not produce the Middle Palace's Ten. Conclusion The combination of respective separations of Sheng-cheng Numbers in Hetu's four directions is the prerequisite of transformation from Hetu to Luoshu. The root of the universe's occurrence is Duidai and Heshi in Luoshu and the root of the universe's development is the separations of Sheng-cheng Numbers from the unity in Hetu.

Congenital Xun-hexagram representing the individual's respiratory gateway at the embryo stage comes from Acquired Kun-hexagram representing the parent thus the health preserving from the home-environment can be deduced Accordingly. The back wall erected, Hill-orientation of the house established; capped already, Floor-disc' Center-palace of the house generated; the family members entered, Sky-disc' Center-palace of the house located; the stove fire started, the Destiny of Earth of the house just when operated.

Congenital Eight Hexagram represents Time in Spreading Sequence and Acquired Eight Hexagram represents Space in Opposite He-ten. Acquired Eight Hexagram is the phased stagnation of Time in the process of Spreading Sequence and Congenital Eight Hexagram is the continued development of Space after the reorganization of Opposite He-ten.Time and Space can be changed to each other. The moment Time towards Space includes the future of Space in Opposite He-ten and the moment Space towards Time includes the future of Time in Spreading Sequence.

Following the transformation of Congenital Eight Hexagram to Acquired Eight Hexagram,the relation between Chou of the Earthy Branches with Jia of the Heavenly Stems was tested and the arrangement rule of the other heavenly stems with their corresponding Tianyi-elegants was also inferred accordingly.We could find that the translocation of Two with Eight in the transformation of Congenital Eight Hexagram to Acquired Eight Hexagram was the axis of the generating process from Middle Palace Five of Acquired Eight Hexagram to Jia of the Heavenly Stems and the rest of the Tianyi-elegants were derived in accordance with their different Heavenly Stems orderly following the Yin-Yang's properties of Hetu's four directions.So we thought that the Tianyi-elegants was originated by the translocation of Two with Eight in the transformation of Congenital Eight Hexagram to Acquired Eight Hexagram.