Objective To explore the application value of the PDCA(Plan-Do-Check-Act)cycle in hospital document supervision management,with the aim of improving document processing efficiency and optimizing administrative workflows.Methods A tertiary hospital in South China was selected as the study subject.In response to a low document completion rate in 2023(74.99％),a fishbone diagram analysis was conducted to identify key efficiency bottlenecks.A classified and hierarchical supervision model based on the PDCA cycle was established.Departments were grouped into high-volume(＞400 cases/year),medium-volume(100-400 cases/year),and low-volume(＜100 cases/year).Targeted interventions were implemented in low-efficiency departments using a"liaison officer+negative list+responsible person tracking"mechanism.Results After im-plementation,the completion rate in low-efficiency departments improved from 26.93％-87.86％ to 58.25％-100％(P=0.028).The hospital's overall average document completion rate reached 93.30％ in 2024,an 18.31％ increase compared to baseline.Conclusion The stratified document supervision model based on the PDCA cycle can significantly enhance administra-tive efficiency and provides practical reference for refined management in public hospitals.

Objective:To explore the biodistribution characteristics of mixed activated killer (MAK) immune cells in immunodeficient mice after administration.Methods:Ninety-six immune immunodeficient (NOG) mice (half male and half female) were equally divided into MAK cell group and solvent control group. The MAK cell group mice were injected with DiR-labeled MAK cells via the tail vein, while those in the solvent control group were injected with an equal amount of solvent via the tail vein. The number of MAK cells in the peripheral blood of mice was detected using a flow cytometry at 11 time points from 15 minutes to 84 days after administration. The distribution of MAK cells in mice was measured using in vivo bioluminescence imaging at 18 time points from 5 minutes to 84 days after administration. And at 8 time points from 3 hours to 84 days after administration, the heart, liver, spleen, lungs, kidneys, brain, stomach, duodenum, colon, bone marrow, fat, skeletal muscle, testes/uterus, epididymis/ovary, and blood were collected from corresponding mice. The DNA levels of MAK cells in blood and various organs of these mice were detected using fluorescence real-time quantitative polymerase chain reaction (qPCR) method.Results:The flow cytometry results showed that MAK cells could be detected in the peripheral blood of mice 15 minutes after administration, and the highest number of MAK cells in blood appeared during 3 hours to 1 day. By 14 days after administration, MAK cells were almost undetectable in peripheral blood of mice. In vivo bioluminescence imaging results showed that the fluorescence intensity of MAK cells in mice was strongest on days 1 and 2 after administration, and MAK cells were mostly distributed in the liver, spleen, lung, and leg bone of mouse. The qPCR detection results showed that MAK cells were mainly distributed in the spleen and lungs. High levels of MAK cell DNA amplification were observed in organs such as the spleen and lungs 28-56 days after administration, and a certain amount of MAK cell DNA could still be detected in organs of mice such as the spleen at 84 days.Conclusions:After administration, MAK cells were mainly distributed in the spleen, lung, liver and other organs of NOG mice. From 28 to 56 days after administration, MAK cells are significantly activated and proliferate, and a certain amount of MAK cell DNA can still be detected in the spleen and other organs after 84 days in mice.

Objective To explore the application value of the PDCA(Plan-Do-Check-Act)cycle in hospital document supervision management,with the aim of improving document processing efficiency and optimizing administrative workflows.Methods A tertiary hospital in South China was selected as the study subject.In response to a low document completion rate in 2023(74.99％),a fishbone diagram analysis was conducted to identify key efficiency bottlenecks.A classified and hierarchical supervision model based on the PDCA cycle was established.Departments were grouped into high-volume(＞400 cases/year),medium-volume(100-400 cases/year),and low-volume(＜100 cases/year).Targeted interventions were implemented in low-efficiency departments using a"liaison officer+negative list+responsible person tracking"mechanism.Results After im-plementation,the completion rate in low-efficiency departments improved from 26.93％-87.86％ to 58.25％-100％(P=0.028).The hospital's overall average document completion rate reached 93.30％ in 2024,an 18.31％ increase compared to baseline.Conclusion The stratified document supervision model based on the PDCA cycle can significantly enhance administra-tive efficiency and provides practical reference for refined management in public hospitals.

Objective:To explore the biodistribution characteristics of mixed activated killer (MAK) immune cells in immunodeficient mice after administration.Methods:Ninety-six immune immunodeficient (NOG) mice (half male and half female) were equally divided into MAK cell group and solvent control group. The MAK cell group mice were injected with DiR-labeled MAK cells via the tail vein, while those in the solvent control group were injected with an equal amount of solvent via the tail vein. The number of MAK cells in the peripheral blood of mice was detected using a flow cytometry at 11 time points from 15 minutes to 84 days after administration. The distribution of MAK cells in mice was measured using in vivo bioluminescence imaging at 18 time points from 5 minutes to 84 days after administration. And at 8 time points from 3 hours to 84 days after administration, the heart, liver, spleen, lungs, kidneys, brain, stomach, duodenum, colon, bone marrow, fat, skeletal muscle, testes/uterus, epididymis/ovary, and blood were collected from corresponding mice. The DNA levels of MAK cells in blood and various organs of these mice were detected using fluorescence real-time quantitative polymerase chain reaction (qPCR) method.Results:The flow cytometry results showed that MAK cells could be detected in the peripheral blood of mice 15 minutes after administration, and the highest number of MAK cells in blood appeared during 3 hours to 1 day. By 14 days after administration, MAK cells were almost undetectable in peripheral blood of mice. In vivo bioluminescence imaging results showed that the fluorescence intensity of MAK cells in mice was strongest on days 1 and 2 after administration, and MAK cells were mostly distributed in the liver, spleen, lung, and leg bone of mouse. The qPCR detection results showed that MAK cells were mainly distributed in the spleen and lungs. High levels of MAK cell DNA amplification were observed in organs such as the spleen and lungs 28-56 days after administration, and a certain amount of MAK cell DNA could still be detected in organs of mice such as the spleen at 84 days.Conclusions:After administration, MAK cells were mainly distributed in the spleen, lung, liver and other organs of NOG mice. From 28 to 56 days after administration, MAK cells are significantly activated and proliferate, and a certain amount of MAK cell DNA can still be detected in the spleen and other organs after 84 days in mice.