Objective To analyze the changes in serum miR-205,miR-23b and miR-25 levels and their correlation with the prognosis of patients with different severities of sepsis.Methods A retrospective study was conducted in 86 patients with sepsis admitted to our hospital between September 2021 and September 2023.Based on the severity of illness,patients were divided into mild(52 cases)and severe(34 cases)groups.Eighty healthy individuals undergoing medical check-ups during the same period were selected as controls.The patients were follo wed-up for 28 days and were then categorized into a survival group(68 patients)and a death group(18 patients).Real-time polymerase chain reaction was used to detect the serum miR-205,miR-23b,and miR-25 levels in patients and analyze their corre-lation with prognosis.Multivariate Cox regression analysis was used to analyze the risk factors affecting patient prognosis.The diagnostic value of serum miR-205,miR-23b,and miR-25 levels in assessing sepsis and their predictive value for prognosis were analyzed using receiver operating characteristic curves.Results Serum miR-205,miR-23b,and miR-25 levels were significantly lower in the two groups of patients with sepsis than in the control group(P＜0.05),and were significantly higher in the survival group than in the death group(P＜0.05).Their levels negatively correlated with prognosis.Heart rate and APACHE Ⅱ score were risk factors for a poor prognosis of patients with sepsis,whereas high serum miR-205,miR-23b,and miR-25 levels were protective factors(P＜0.05).The areas under the curve(AUC)for individual serum miR-205,miR-23b,and miR-25 levels and their combination to assess sepsis were 0.794,0.786,0.768,and 0.926,respectively.The AUC for predicting poor prognosis for patients were 0.776,0.762,0.797,and 0.918,respectively.Conclusion Serum miR-205,miR-23b,and miR-25 levels were reduced in patients with sepsis,and were closely correlated with patient prognosis.Thus,they may serve as serological biomarkers for assessing disease and predicting prognosis.

Objective To analyze the changes in serum miR-205,miR-23b and miR-25 levels and their correlation with the prognosis of patients with different severities of sepsis.Methods A retrospective study was conducted in 86 patients with sepsis admitted to our hospital between September 2021 and September 2023.Based on the severity of illness,patients were divided into mild(52 cases)and severe(34 cases)groups.Eighty healthy individuals undergoing medical check-ups during the same period were selected as controls.The patients were follo wed-up for 28 days and were then categorized into a survival group(68 patients)and a death group(18 patients).Real-time polymerase chain reaction was used to detect the serum miR-205,miR-23b,and miR-25 levels in patients and analyze their corre-lation with prognosis.Multivariate Cox regression analysis was used to analyze the risk factors affecting patient prognosis.The diagnostic value of serum miR-205,miR-23b,and miR-25 levels in assessing sepsis and their predictive value for prognosis were analyzed using receiver operating characteristic curves.Results Serum miR-205,miR-23b,and miR-25 levels were significantly lower in the two groups of patients with sepsis than in the control group(P＜0.05),and were significantly higher in the survival group than in the death group(P＜0.05).Their levels negatively correlated with prognosis.Heart rate and APACHE Ⅱ score were risk factors for a poor prognosis of patients with sepsis,whereas high serum miR-205,miR-23b,and miR-25 levels were protective factors(P＜0.05).The areas under the curve(AUC)for individual serum miR-205,miR-23b,and miR-25 levels and their combination to assess sepsis were 0.794,0.786,0.768,and 0.926,respectively.The AUC for predicting poor prognosis for patients were 0.776,0.762,0.797,and 0.918,respectively.Conclusion Serum miR-205,miR-23b,and miR-25 levels were reduced in patients with sepsis,and were closely correlated with patient prognosis.Thus,they may serve as serological biomarkers for assessing disease and predicting prognosis.

Objective:To assess the changes of selenium nutrition of school-age children in Kashin-Beck disease (KBD) areas of Linzhou County, Lhasa City and Xietongmen County, Shigatse City in Tibet Autonomous Region (referred to as Tibet), and provide a scientific basis for evaluating the effectiveness of prevention and control measures.Methods:According to the historical condition of KBD, a total of 344 children's hair samples were collected to determine the content of selenium in Kazi (KBD area) and Jiangxia townships (non-KBD area) of Linzhou County in 2013 and 2021, Renqinze (KBD area) and Tongmen townships (non-KBD area) of Xietongmen County in 2015 and 2021.Results:Compared to 2013/2015, in 2021, the hair selenium level of children in the four townships increased ( P < 0.001). The selenium nutritional level of more than 90% of the children reached medium or above (hair selenium > 0.25 μg/g) in 2021. The hair selenium levels of girls in the two KBD areas (Kazi and Renqinze townships) were lower than those of boys ( Z = - 2.83, - 2.83, P < 0.05). Conclusions:The selenium nutrition level of school-age children in KBD areas in Linzhou and Xietongmen counties has increased rapidly in recent years. However, the selenium nutrition level of girls is significantly lower than that of boys. It is necessary to strengthen prevention, controlling and monitoring, and to further improve the dietary structure of school-age children through the joint efforts of families and schools, to increase the proportion of exogenous high selenium food intake.

Objective:To assess the present situation of selenium nutrition in adults and school-age children in Kaschin-Beck disease area of Binxian, Shaanxi Province, and provide the basis for taking targeted measures.Methods:Binxian was divided into five grades according to the condition of Kaschin-Beck disease, and 11 representative villages were selected as the survey villages in 2018. Each survey village was located in five directions: east, west, south, north, and middle. Two household villagers were selected from each location, and adult hair samples were collected for hair selenium content determination. And school-age children hair samples were collected at the township central primary schools and 1 village primary school corresponding to the five ward levels, and the selenium content was determined.Results:A total of 85 adult hair samples were collected, the average selenium content was 237.1 μg/kg, generally at the marginal selenium deficiency level (200 - < 250 μg/kg), and there was no significant difference among different grades of disease areas (χ 2 = 5.616, P > 0.05) . A total of 162 children hair samples were collected, the average selenium content was 301.3 μg/kg, generally at the middle selenium nutrition level (250 - < 500 μg/kg), and there was significant difference among different grades of disease areas (χ 2 = 13.627, P < 0.05) . Conclusion:Although Binxian has got rid of the risk of Kaschin-Beck disease as a whole, the selenium nutrition levels of some adults and children are still low, so it is still necessary to strengthen the prevention and control measures, especially for school-age children living in a relatively serious historical disease villages.

Objective To study the relationship between hair selenium content and arsenism among population from endemic arsenism area caused by coal combustion in the southern of Shaanxi Province.Methods Four high-arsenic-exposed villages in southern Shaanxi Province were selected as the study area,and the nonarsenic-exposed villages nearby were selected as the control area,90 hair samples of residents exposed to high arsenic in the study area and 14 hair samples of residents in the control area were collected.Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to determine the contents of arsenic and selenium in hair samples.Selenium/hair arsenic (Se/As) value was calculated,and the relationship between selenium content in hair samples and arsenicosis was analyzed.Results The geometric mean of selenium concentration in hair from the study area was 8.72 mg/kg,which was significandy higher than that from the control area (0.54 mg/kg,t =9.811,P ＜ 0.05).However,selenosis case was not found in the study area.Selenium content in hair was not obviously correlated with arsenism (r =0.075,P ＞ 0.05),while the Se/As value was negatively associated with the grade of arsenism (P ＞ 0.05).The geometric mean of selenium content in hair of female from the study area was 12.79 mg/kg,which was higher than that in hair of male (6.5 mg/kg,t =1.738,P ＜ 0.01).Meanwhile,the Se/As value in hair of female from the study area (1.15) was significantly higher than that in hair of male (0.65,t =3.218,P ＜ 0.05).Conclusions The environment of the study area is polluted by arsenic and selenium derived from stone-coal burning.Antagonism between arsenic and selenium might reduce the toxicity of selenium.Therefore,selenosis is not found in the study area.In addition,antagonism between arsenic and selenium might also induce lower prevalence of arsenism for female in the studly area.

OBJECTIVE:To study the protective effects of total flavone from Prunus cerasifera fruits(PCE)on alcoholic liver disease(ALD)in rats,and provide experimental basis for developing new medicines for anti-ALD. METHODS:40 rats were ran-domly divided into normal group(distilled water),model group(distilled water),silibinin group [positive control,30 mg/(kg·d)] and PCE high-dose,low-dose groups [80,40 mg/(kg·d)],8 in each group. All rats were intragastrically administrated(10 mL/kg) every morning,once,for 6 weeks;meanwhile,except for normal group,rats in other groups received 50% alcohol(10 mL/kg) once intragastrically every afternoon to induce ALD model. After administration,heart,liver,spleen and other organ indexes,sub-cutaneous fat,brown fat,abdominal fat indexes were determined,as well as serum biochemical indexes [glutamate transaminase (ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),direct bilirubin(DBIL),indirect bilirubin(IBIL),gamma-glu-tamyl transpeptidase(GGT),and total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipopro-tein (LDL)] level and liver biochemical indexes [superoxide dismutase (SOD),malondialdehyde (MDA),glutathione peroxidase (GSH-Px),TC,TG] and TC,TG levels in feces;pathological changes of liver and kidney tissues were observed. RESULTS:Compared with normal group,heart,liver,spleen indexes,subcutaneous fat,abdominal fat indexes in model group were in-creased(P<0.05 or P<0.01),brain index and brown fat index were decreased(P<0.05);HDL level and HDL/TC ratio in serum were decreased,other serum indexes were increased(P<0.05 or P<0.01);SOD,GSH-Px levels in liver tissue were decreased, other above-mentioned liver biochemical indexes were increased(P<0.01);TC,TG levels in feces were increased(P<0.01);liv-er and kidney showed obvious lesions. Compared with model group,the above-mentioned indexes in silibinin group and PCE high-dose group were significantly improved (P<0.05 or P<0.01);ALT,AST in serum and MDA level in liver tissue in PCE low-dose group were significantly decreased(P<0.01),and SOD,GSH-Px levels in liver tissue were significantly increased(P<0.05);lesion degree of liver and kidney and lipid accumulation in liver were reduced in administration groups. CONCLUSIONS:PCE may play a role in anti-ALD by anti-oxidation,promoting liver cell regeneration and regulating lipid metabolism.