Benign metastasizing leiomyomatosis is a rare clinical entity characterized by benign leiomyomas occur-ring outside the uterus,with the lungs being the most commonly involved site,thus termed pulmonary benign metastasi-zing leiomyomatosis.Fumarate hydratase(FH)-deficient leiomyoma represents a rare pathological variant of uterine leiomyoma,which is of great significance for screening hereditary leiomyomatosis and renal cell carcinoma syndrome.Although there have been reports of FH-deficient leiomyomas involving the lungs,studies on FH-deficient pulmonary benign metastasizing leiomyoma in this context remain limited.This article offers a comprehensive review of existing studies on FH-deficient pulmonary benign metastasizing leiomyoma by integrating the epidemiology,clinical manifesta-tions,pathological features,diagnosis,differential diagnosis,and therapeutic advancements of primary uterine FH-de-ficient leiomyoma.

Benign metastasizing leiomyomatosis is a rare clinical entity characterized by benign leiomyomas occur-ring outside the uterus,with the lungs being the most commonly involved site,thus termed pulmonary benign metastasi-zing leiomyomatosis.Fumarate hydratase(FH)-deficient leiomyoma represents a rare pathological variant of uterine leiomyoma,which is of great significance for screening hereditary leiomyomatosis and renal cell carcinoma syndrome.Although there have been reports of FH-deficient leiomyomas involving the lungs,studies on FH-deficient pulmonary benign metastasizing leiomyoma in this context remain limited.This article offers a comprehensive review of existing studies on FH-deficient pulmonary benign metastasizing leiomyoma by integrating the epidemiology,clinical manifesta-tions,pathological features,diagnosis,differential diagnosis,and therapeutic advancements of primary uterine FH-de-ficient leiomyoma.

OBJECTIVE To investigate the effect of jujuboside A on glomerular cell apoptosis in diabetic rats, and to explore the possible mechanisms. METHODS SD rats were administered with streptozotocin 100 mg · kg-1 to estabilish the diabetic model. Diabetic SD rats received jujuboside A 10 and 20 mg · kg-1 daily for 4 weeks by lavage administration, respectively. The level of glycosylated hemoglobin (GHb) in the blood of each group was measured by fructosamine method. The morphological changes in glomerular cells were observed by PAS staining. Glomerular cell apoptosis was determined by TUNEL staining. The protein expression of Bcl-2 and Bax was detected by immunohistochemistry. The protein expression of cleaved caspase 9 and cleaved caspase 3 was detected by Western blotting. Trans?forming growth factor β1 (TGF-β1) mRNA expression was analyzed by qPCR. RESULTS Compared with model group, jujuboside A 10 and 20 mg·kg-1 treatment significantly reduced the level of GHb in blood (mmol · L- 1: 10.9 ± 0.8 vs 17.5 ± 1.5, P<0.05; 7.6 ± 0.5 vs 17.5 ± 1.5, P<0.05), PAS positive score of glomerular cells (26.8 ± 3.2 vs 36.4 ± 3.8, P<0.05; 18.4 ± 2.1 vs 36.4 ± 3.8, P<0.05) and the apoptosis of glomerular cells〔(8.2±0.8)%vs (17.6±1.8)%, P<0.05;(5.1±0.5)%vs (17.6±1.8)%, P<0.05〕. Moreover, Bcl-2 protein expression in kidney tissues was elevated (P<0.05), whereas Bax (P<0.05), cleaved caspase 9 (P<0.05) and cleaved caspase 3 (P<0.05) protein expression and TGF-β1 mRNA (P<0.05) expression were reduced after jujuboside A administration. CONCLUSION Jujuboside A can prevent glomerular cell apoptosis in diabetic rats, which may be associated with the regulation of mitochondrial apoptotic pathways and TGF-β1 expression.

We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.
Base Sequence ; China ; Cluster Analysis ; Gene Components ; Genetic Variation ; Genome, Viral ; Genotype ; Molecular Sequence Data ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; SARS Virus ; genetics ; Sequence Analysis, DNA ; Severe Acute Respiratory Syndrome ; genetics