Objective:To observe the changes of small airway parameters in patients with coal workers' pneumoconiosis in different disease stages by high resolution computed tomography (HRCT) , and analyze the correlation between them and the severity of the disease.Methods:From June 2016 to June 2023, 25 healthy volunteers and 71 untreated patients with coal worker's pneumoconiosis in the Fifth People's Hospital of Ningxia were selected as the research objects. The clinical and imaging data of the patients were collected, and the disease stages were performed according to the dust exposure history and high-kilovolt chest X-ray. The patients were divided into 4 groups: control group (25 cases) , coal workers' pneumoconiosis stage Ⅰ group (17 cases) , coal workers' pneumoconiosis stage Ⅱ group (32 cases) and coal workers' pneumoconiosis stage Ⅲ group (22 cases) . Quantitative chest HRCT parameters of each group were collected, including the square root of wall area at 10 mm inner perimeter (AWT-Pi10, Pi10) , airway wall thickness, airway wall volume, airway wall area percentage of the whole lung and the 5th, 6th, 7th and 8th level airways, and low attenuation area percentage (LAA%) of the whole lung. Pulmonary function indicators were collected, including forced expiratory volume in 1 second (FEV 1) and the percentage of its projected value [FEV 1 (%pred) ], the ratio of FEV 1 to forced vital capacity (FEV 1/FVC) and the percentage of its projected value[FEV 1/FVC (%pred) ]. One-way ANOVA or Kruskal-Wallis H test and Spearman rank correlation were used to analyze the difference and correlation. Results:Compared with control group, FEV 1, FEV 1 (%pred) , FEV 1/FVC and FEV 1/FVC (%pred) in stage Ⅱ and Ⅲ coal workers' pneumoconiosis groups were lower ( P<0.05) . In addition, the FEV 1 and FEV 1 (%pred) of the stage Ⅲgroup were lower than those of the stageⅡ group ( P<0.05) , and the FEV 1/FVC and FEV 1/FVC (%pred) of the stage Ⅲgroup were lower than those of the stage Ⅰgroup ( P<0.05) . Compared with stage Ⅰ group, Pi10 in stage Ⅲ group were increased ( P < 0.05) at the 6th and 8th level airways, and airway wall thickness and airway wall volume in the 6th, 7th and 8th level airways of stage Ⅲgroup increased ( P<0.05) . Correlation analysis showed that all pulmonary function indexes were negatively correlated with Pi10 of whole lung and the 6th, 7th and 8th level airways ( P<0.05) , all pulmonary function indexes were negatively correlated with airway wall thickness of the 7th and 8th level airways ( P<0.05) , and FEV 1/FVC (%pred) was negatively correlated with airway wall volume of the 7th and 8th level airways ( P<0.05) . FEV 1, FEV 1 (%pred) , FEV 1/FVC (%pred) were negatively correlated with percentage of airway wall area of whole lung and the 6th, 7th and 8th level airways ( P<0.05) . Conclusion:The quantitative airway parameters of coal workers' pneumoconiosis based on HRCT are correlated with pulmonary function indexes, which can reflect the severity of coal workers' pneumoconiosis.

Objective To use imaging features of pulmonary nodules to predict the risk of lymph node metastasis in patients with cT1-stage non-small cell lung cancer (NSCLC), providing a reference for clinical decision-making. Methods A retrospective analysis was conducted on the imaging features and postoperative pathological results of cT1 NSCLC patients who underwent surgical treatment at Linyi People’s Hospital from July 2019 to July 2022. Patients were grouped and analyzed according to lymph node metastasis status. Results A total of 1 123 patients were included, comprising 471 males and 652 females, with a median age of 59 (52, 66) years. Comparative analysis revealed that sex, age, nodule location, nodule size on imaging, solid component size, consolidation tumor ratio (CTR), average CT value, and tumor proximity to the pleura all influenced lymph node metastasis. A nomogram was constructed, indicating that the probability of lymph node metastasis in cT1 NSCLC was positively correlated with solid component size, CTR, and average CT value of the pulmonary nodule, and negatively correlated with patient age. The area under the receiver operating characteristic curve was 0.929. Conclusion For cT1 NSCLC patients, the probability of lymph node metastasis can be predicted by measuring the solid component size, CTR, and average CT value of the pulmonary nodule, in conjunction with patient age. However, relying solely on pulmonary nodule imaging characteristics is insufficient to determine a specific lymph node dissection strategy.

Objective:To explore the effects of LncRNA DLEU2 on the migration and proliferation of oral squamous cell carcino-ma(OSCC)cells by regulating the miR-186-5p/insulin-like growth factor 2 mRNA binding protein 3(IGF2BP3)axis.Methods:QRT-PCR and Western blot were applied to detect the mRNA and protein levels of LncRNA DLEU2,miR-186-5p,and IGF2BP3 in OSCC cell line SCC-25 and human normal oral keratinocyte line NOK,respectively.SCC-25 cells were transfected with si-DLEU2,miR-186-5p inhibitor and negative control respectively.The relationship between LncRNA DLEU2,miR-186-5p and IGF2BP3 was verified by double luciferase reporter gene assay.qRT-PCR was applied to detect the mRNA expression of LncRNA DLEU2 and miR-186-5p in SCC-25 cells.CCK-8 test was applied to detect the proliferation of SCC-25 cells.Flow cytometry was applied to detect the apoptosis rate of SCC-25 cells.Transwell assay was used to detect cell invasion and migration.Western blot was used to detect EMT-associated and IGF2BP3 protein levels.Results:Silencing LncRNA DLEU2 decreased the proliferative ac-tivity,migration and invasion of SCC-25 cells and decreased the protein levels of N-cadherin and Vimentin,increased the apopto-sis rate,miR-186-5p expression and E-cadherin protein levels.Down-regulation of miR-186-5p weakened the inhibitory effect of silenced LncRNA DLEU2 on the malignant phenotype of SCC-25 cells.LncRNA DLEU2 negatively regulated the miR-186-5p/IGF2BP3 axis.Conclusion:Silencing LncRNA DLEU2 may down-regulate the expression of IGF2BP3 by up-regulating miR-186-5p,inhibites the proliferation,migration,and invasion of SCC-25 cells,and promotes the apoptosis of the cells.

Continuous care can effectively improve the quality of life, self-management ability and health outcomes of lung transplant recipients, and is important for reducing readmission rates and medical costs, but there are no standardized criteria for evaluating the quality of continuous care for lung transplant recipients. Based on the Donabedian three-dimensional quality evaluation model, this paper presents a review of the indicators for evaluating the quality of continuous care for lung transplant recipients, and analyses the current state of the art of assessing the quality of care in the current literature, in order to provide reference for the development of a multi-dimensional, highly sensitive and specific evaluation index system for lung transplant recipients′ continuous care, as well as providing a direction for future research and clinical application.

Objective:To explore the effects of LncRNA DLEU2 on the migration and proliferation of oral squamous cell carcino-ma(OSCC)cells by regulating the miR-186-5p/insulin-like growth factor 2 mRNA binding protein 3(IGF2BP3)axis.Methods:QRT-PCR and Western blot were applied to detect the mRNA and protein levels of LncRNA DLEU2,miR-186-5p,and IGF2BP3 in OSCC cell line SCC-25 and human normal oral keratinocyte line NOK,respectively.SCC-25 cells were transfected with si-DLEU2,miR-186-5p inhibitor and negative control respectively.The relationship between LncRNA DLEU2,miR-186-5p and IGF2BP3 was verified by double luciferase reporter gene assay.qRT-PCR was applied to detect the mRNA expression of LncRNA DLEU2 and miR-186-5p in SCC-25 cells.CCK-8 test was applied to detect the proliferation of SCC-25 cells.Flow cytometry was applied to detect the apoptosis rate of SCC-25 cells.Transwell assay was used to detect cell invasion and migration.Western blot was used to detect EMT-associated and IGF2BP3 protein levels.Results:Silencing LncRNA DLEU2 decreased the proliferative ac-tivity,migration and invasion of SCC-25 cells and decreased the protein levels of N-cadherin and Vimentin,increased the apopto-sis rate,miR-186-5p expression and E-cadherin protein levels.Down-regulation of miR-186-5p weakened the inhibitory effect of silenced LncRNA DLEU2 on the malignant phenotype of SCC-25 cells.LncRNA DLEU2 negatively regulated the miR-186-5p/IGF2BP3 axis.Conclusion:Silencing LncRNA DLEU2 may down-regulate the expression of IGF2BP3 by up-regulating miR-186-5p,inhibites the proliferation,migration,and invasion of SCC-25 cells,and promotes the apoptosis of the cells.

Continuous care can effectively improve the quality of life, self-management ability and health outcomes of lung transplant recipients, and is important for reducing readmission rates and medical costs, but there are no standardized criteria for evaluating the quality of continuous care for lung transplant recipients. Based on the Donabedian three-dimensional quality evaluation model, this paper presents a review of the indicators for evaluating the quality of continuous care for lung transplant recipients, and analyses the current state of the art of assessing the quality of care in the current literature, in order to provide reference for the development of a multi-dimensional, highly sensitive and specific evaluation index system for lung transplant recipients′ continuous care, as well as providing a direction for future research and clinical application.

Objective:To observe the changes of small airway parameters in patients with coal workers' pneumoconiosis in different disease stages by high resolution computed tomography (HRCT) , and analyze the correlation between them and the severity of the disease.Methods:From June 2016 to June 2023, 25 healthy volunteers and 71 untreated patients with coal worker's pneumoconiosis in the Fifth People's Hospital of Ningxia were selected as the research objects. The clinical and imaging data of the patients were collected, and the disease stages were performed according to the dust exposure history and high-kilovolt chest X-ray. The patients were divided into 4 groups: control group (25 cases) , coal workers' pneumoconiosis stage Ⅰ group (17 cases) , coal workers' pneumoconiosis stage Ⅱ group (32 cases) and coal workers' pneumoconiosis stage Ⅲ group (22 cases) . Quantitative chest HRCT parameters of each group were collected, including the square root of wall area at 10 mm inner perimeter (AWT-Pi10, Pi10) , airway wall thickness, airway wall volume, airway wall area percentage of the whole lung and the 5th, 6th, 7th and 8th level airways, and low attenuation area percentage (LAA%) of the whole lung. Pulmonary function indicators were collected, including forced expiratory volume in 1 second (FEV 1) and the percentage of its projected value [FEV 1 (%pred) ], the ratio of FEV 1 to forced vital capacity (FEV 1/FVC) and the percentage of its projected value[FEV 1/FVC (%pred) ]. One-way ANOVA or Kruskal-Wallis H test and Spearman rank correlation were used to analyze the difference and correlation. Results:Compared with control group, FEV 1, FEV 1 (%pred) , FEV 1/FVC and FEV 1/FVC (%pred) in stage Ⅱ and Ⅲ coal workers' pneumoconiosis groups were lower ( P<0.05) . In addition, the FEV 1 and FEV 1 (%pred) of the stage Ⅲgroup were lower than those of the stageⅡ group ( P<0.05) , and the FEV 1/FVC and FEV 1/FVC (%pred) of the stage Ⅲgroup were lower than those of the stage Ⅰgroup ( P<0.05) . Compared with stage Ⅰ group, Pi10 in stage Ⅲ group were increased ( P < 0.05) at the 6th and 8th level airways, and airway wall thickness and airway wall volume in the 6th, 7th and 8th level airways of stage Ⅲgroup increased ( P<0.05) . Correlation analysis showed that all pulmonary function indexes were negatively correlated with Pi10 of whole lung and the 6th, 7th and 8th level airways ( P<0.05) , all pulmonary function indexes were negatively correlated with airway wall thickness of the 7th and 8th level airways ( P<0.05) , and FEV 1/FVC (%pred) was negatively correlated with airway wall volume of the 7th and 8th level airways ( P<0.05) . FEV 1, FEV 1 (%pred) , FEV 1/FVC (%pred) were negatively correlated with percentage of airway wall area of whole lung and the 6th, 7th and 8th level airways ( P<0.05) . Conclusion:The quantitative airway parameters of coal workers' pneumoconiosis based on HRCT are correlated with pulmonary function indexes, which can reflect the severity of coal workers' pneumoconiosis.

This article has found that acupuncture can treat vascular cognitive impairment (VCI) through alleviating nerve cell apoptosis, enhancing hippocampal synaptic plasticity, improving hippocampal mitochondrial function, reducing brain inflammation, inhibiting oxidative stress response, protecting the blood-brain barrier, promoting angiogenesis, improving cerebral circulation, enhancing default network connectivity in brain regions, and improving white matter damage. At present, it is still in the preliminary stage to explore the mechanism of acupuncture treatment of VCI from the perspective of brain network connection. The existing mechanism researches mostly use electroacupuncture as an intervention method, and the selection of acupoints and the intensity of electroacupuncture stimulation are different. It is necessary to improve the rigor of the experiment, strengthen the study of conventional acupuncture, and explore the operation methods and needle retention time. The existing animal models lack consideration of other complications of VCI, and there are still some differences with the clinical characteristics of VCI patients, which need to be optimized in the future.

Objective:This study aimed to construct a risk prediction model for obstructive sleep apnea（OSA） related hypertension based on the nomogram, and to explore the independent risk factors for OSA-related hypertension, so as to provide reference for clinical treatment decision-making. Methods:The clinical data of OSA patients diagnosed by polysomnography from October 2019 to December 2021 were collected retrospectively and randomly divided into training sets and validation sets. A total of 1 493 OSA patients with 27 variables were included. The least absolute shrinkage and selection operator（Lasso） logistic regression model was used to select potentially relevant features and establish a nomogram for OSA-related hypertension.The performance and clinical benefits of this nomogram were verified in terms of discrimination, calibration ability and clinical net benefit. Results:Multivariate logistic regression showed that body mass index（BMI）, family history of hypertension, lowest oxygen saturation（LSaO2）, age and cumulative percentage of total sleep time with oxygen saturation below 90% were independent risk factors for OSA-related hypertension. Lasso logistic regression identified BMI, family history of hypertension, LSaO2 and age as predictive factors for inclusion in the nomogram. The nomogram provided a favorable discrimination, with a C-indexes of 0.835（95% confidence interval[CI ]0.806-0.863） 0.865（95%CI 0.829-0.900） for the training and validation cohort, respectively, and well calibrated. The clinical decision curve analysis displayed that the nomogram was clinically useful. Conclusion:Compared with cumulative percentage of total sleep time with blood oxygen saturation below 90%, LSaO2 may have a greater impact on the incidence of OSA-related hypertension, and the effects of different times and degrees of hypoxia on OSA-related hypertension should be further explored in the future. Apnea hypopnea index involvement is weak in predicting OSA-related hypertension, and the blood oxygen index may be a better predictor variable. Furthermore, we established a risk prediction model for OSA-related hypertension patients using nomogram, and demonstrated that this prediction model was helpful to identify high-risk OSA-related hypertension patients. This model can provide early and individualized diagnosis and treatment plans, protect patients from the serious.
Humans ; Sleep Apnea, Obstructive/complications* ; Nomograms ; Hypertension/epidemiology* ; Male ; Female ; Risk Factors ; Middle Aged ; Retrospective Studies ; Polysomnography ; Logistic Models ; Body Mass Index ; Adult

Objective To develop a symptoms assessment scale for lung transplant recipients in early postoperative period,and assess its reliability and validity so as to provide clinical staff with a tool to evaluate the symptoms in the patients in the early period after lung transplantation.Methods With a symptom experience model,a preliminary item pool was established through literature reviews,semi-structured interviews and expert panel meetings between December 2021 and January 2022.Based on the preliminary item pool,an initial scale was proposed after two rounds of expert consultation between February and March 2022.The initial scale was finalised after all items had revised for language expression based on the results of cognitive interviews conducted in March 2022.Subsequently,the final version of the scale was applied in the survey of 116 recipients of lung transplant in the early postoperative stage at Wuxi People's Hospital between April 2022 and February 2023.The reliability and validity of scale were then further tested.Results A total of 112 patients responded to the questionnaire survey.The developed scale comprised 5 dimensions:psychology-related symptoms,respiratory related symptoms,digestive related symptoms,circulatory related symptoms and other symptoms,with 18 symptom items in total.Content validity indexes of the Item-level content validity index(I-CVI))were 0.833 to 1.000 and the Scale-level content validity index(S-CVI)was at 0.954.Exploratory factor analysis revealed five common factors with a cumulative variance contribution rate of 82.366%.All the factors exhibited positive correlations with the scale,with the correlation coefficient at 0.760 to 0.837(P<0.01).The scale demonstrated a Cronbach α coefficient of 0.943,and the Cronbach α coefficients of the five common factors ranged from 0.869 to 0.941.Additionally,the scale exhibited a split-half reliability of 0.840,and the split-half reliability of the five common factors ranged from 0.830 to 0.937.Conclusions The symptoms assessment scale for lung transplant recipients in early postoperative period developed in this study exhibits good reliability and validity.It is feasible for evaluation of early symptoms in lung transplant recipients.