Objective:To verify the applicability of the flight potential evaluation system in the psychological selection of pilots by testing the reliability and validity of the system.Methods:Between September and October 2021, 82 subjects, including 32 pilots and 50 volunteers, were recruited from the Aviation Force and the Air Force Medical Center to complete the flight potential evaluation system test. The pilots and volunteers were divided into the high score group (the top 27% in terms of scores) and low score group (the 27% from the bottom) according to the total score of the evaluation. In order to ensure the consistency of evaluation conditions, some subjects were selected to complete a second evaluation test one week later for reliability analysis. Six flight experts completed the Content Evaluation Form of the Flight Potential Evaluation System while the officer completed the Flight Performance Evaluation Questionnaire as the performance data of the pilots. The pass rates of the pilots and volunteers and discriminability of the flight potential evaluation system were analyzed to test the stability and effectiveness of the system.Results:①The flight potential evaluation system demonstrated a pass rate of 0.75 and a discriminability of 0.30 in pilots, compared with 0.30 and 0.51 in volunteers. The total scores of pilots and volunteers were (7.00±1.16) points and (3.38±2.15) points, respectively, with a statistically significant difference ( t=9.87, P<0.001). Significant differences were found in test scores between high-score group and low-score group for both pilots ( t=10.01, P<0.001) and volunteers ( Z=-4.65, P<0.001). ②Thirty-seven subjects (including 32 pilots and 5 volunteers, all from the Aviation Force) were tested twice, and the Pearson product-moment correlation coefficient for the paired results of 2 identical tests was r=0.750 ( P<0.001). ③As for the content of the evaluation system test, the experts′ degree of agreement and unanimity rate were 1. The evaluation score by the officer was positively correlated with the test score ( r=0.389, P=0.041). Conclusions:The flight potential evaluation system has a strong ability to distinguish flight-related abilities, suggesting that the system can be applied to the psychological selection of pilots and provide data for subsequent tests during the recruitment of candidates.

Objective:To explore the impact of intermittent oro-esophageal tube feeding (IOE) on the nutritional status and prognosis of patients with dysphagia after acute ischemic stroke.Methods:Using the convenience sampling method, 210 patients with dysphagia after acute ischemic stroke who were treated at The Second Affiliated Hospital of Wenzhou Medical University from May 2020 to May 2022 were selected as the research objects. The patients were divided into an observation group ( n=108) and a control group ( n=102) by the coin-tossing method. The control group was intervened with indwelling nasogastric tube method, while the observation group was treated with IOE. The clinical efficacy, nutritional indicators, scores of the Activities of Daily Living (ADL) Scale, and the incidence of complications were compared between the two groups. Results:After the intervention, the total effective rate of the observation group was higher than that of the control group, and the levels of albumin, pre-albumin, and hemoglobin in the observation group were higher than those in the control group, and the incidence of complications was lower than that of the control group. The differences were all statistically significant ( P<0.05) . Conclusions:For patients with acute ischemic stroke, IOE can improve the symptoms of dysphagia, enhance their nutritional level, and also reduce the incidence of complications.

Objective:To explore the impact of intermittent oro-esophageal tube feeding (IOE) on the nutritional status and prognosis of patients with dysphagia after acute ischemic stroke.Methods:Using the convenience sampling method, 210 patients with dysphagia after acute ischemic stroke who were treated at The Second Affiliated Hospital of Wenzhou Medical University from May 2020 to May 2022 were selected as the research objects. The patients were divided into an observation group ( n=108) and a control group ( n=102) by the coin-tossing method. The control group was intervened with indwelling nasogastric tube method, while the observation group was treated with IOE. The clinical efficacy, nutritional indicators, scores of the Activities of Daily Living (ADL) Scale, and the incidence of complications were compared between the two groups. Results:After the intervention, the total effective rate of the observation group was higher than that of the control group, and the levels of albumin, pre-albumin, and hemoglobin in the observation group were higher than those in the control group, and the incidence of complications was lower than that of the control group. The differences were all statistically significant ( P<0.05) . Conclusions:For patients with acute ischemic stroke, IOE can improve the symptoms of dysphagia, enhance their nutritional level, and also reduce the incidence of complications.

Objective:To verify the applicability of the flight potential evaluation system in the psychological selection of pilots by testing the reliability and validity of the system.Methods:Between September and October 2021, 82 subjects, including 32 pilots and 50 volunteers, were recruited from the Aviation Force and the Air Force Medical Center to complete the flight potential evaluation system test. The pilots and volunteers were divided into the high score group (the top 27% in terms of scores) and low score group (the 27% from the bottom) according to the total score of the evaluation. In order to ensure the consistency of evaluation conditions, some subjects were selected to complete a second evaluation test one week later for reliability analysis. Six flight experts completed the Content Evaluation Form of the Flight Potential Evaluation System while the officer completed the Flight Performance Evaluation Questionnaire as the performance data of the pilots. The pass rates of the pilots and volunteers and discriminability of the flight potential evaluation system were analyzed to test the stability and effectiveness of the system.Results:①The flight potential evaluation system demonstrated a pass rate of 0.75 and a discriminability of 0.30 in pilots, compared with 0.30 and 0.51 in volunteers. The total scores of pilots and volunteers were (7.00±1.16) points and (3.38±2.15) points, respectively, with a statistically significant difference ( t=9.87, P<0.001). Significant differences were found in test scores between high-score group and low-score group for both pilots ( t=10.01, P<0.001) and volunteers ( Z=-4.65, P<0.001). ②Thirty-seven subjects (including 32 pilots and 5 volunteers, all from the Aviation Force) were tested twice, and the Pearson product-moment correlation coefficient for the paired results of 2 identical tests was r=0.750 ( P<0.001). ③As for the content of the evaluation system test, the experts′ degree of agreement and unanimity rate were 1. The evaluation score by the officer was positively correlated with the test score ( r=0.389, P=0.041). Conclusions:The flight potential evaluation system has a strong ability to distinguish flight-related abilities, suggesting that the system can be applied to the psychological selection of pilots and provide data for subsequent tests during the recruitment of candidates.

Objective:To investigate the application of debate-based immersive experiential teaching in the nursing practice teaching of stomatology.Methods:A total of 82 nursing interns of stomatology in Beijing Tongren Hospital, Capital Medical University, were selected as research subjects and were then divided into control group and observation group using the lottery method, with 41 interns in each group. The interns in the control group received traditional teaching, while those in the observation group received debate-based immersive experiential teaching. The two groups were compared in terms of the scores of comprehensive evaluation indices, nursing thinking ability, empathy ability, and recognition of teaching. SPSS 22.0 was used to perform the chi-square test and the t-test. Results:Compared with the control group, the observation group had significant increases in the scores of theoretical examination, operation examination, nursing medical record writing, reading report writing, basic nursing, communication ability, and comprehensive performance and the total score of these dimensions ( P<0.05). Compared with the control group, the observation group also had significant increases in the scores of nursing thinking ability and empathy ability ( P<0.05). The observation group had a significantly higher degree of recognition of teaching than the control group ( P<0.05). Conclusion:In the nursing practice teaching of stomatology, debate-based immersive experiential teaching can improve the comprehensive ability, nursing thinking ability, and empathy ability of interns, with a relatively high degree of recognition of teaching.

Objective:To translate the abbreviated Comprehensive Geriatric Assessment (aCGA) into Chinese and test its reliability and validity.Methods:According to the guideline of instruments or scales for use in cross-cultural health care research, the English version of aCGA was translated correctly, back translated, and cross-cultural adapted, ultimately forming the Chinese version of aCGA. From February to July 2021, 275 elderly cancer patients from three departments of Affiliated Hospital of Nantong University were selected as the study subject by convenience sampling. Patients were surveyed using the General Information Questionnaire, Chinese version of aCGA, and the Fried Frailty Phenotype. SPSS 25.0 and AMOS 24.0 software were used to test the reliability and validity of the questionnaire.Results:The Chinese version of aCGA consisted of three dimensions and 15 items. The Cronbach's α coefficient of the questionnaire was 0.806, and the coefficients of depression, function, and cognition dimensions were 0.919, 0.957, and 0.806, respectively. The Kappa value for consistency between raters was 0.778. The content validity index at the item level was 0.833 to 1.000, and the content validity index at the questionnaire level was 0.943. Exploratory factor analysis identified three common factors, with a cumulative variance contribution rate of 81.181%. The evaluation results of the questionnaire was strongly correlated with the Fried Frailty Phenotype, and the difference was statistically significant ( r=0.629, P<0.01) . Conclusions:The Chinese version of aCGA has good reliability, validity, and screening performance, making it suitable for preliminary screening of frailty in elderly cancer patients.

Objective To explore the effect of team-based outpatient education on blood pressure and self management ability of young and middle-aged patients. Methods About 88 cases of middle-aged and young people with high blood pressure from October 2015 to March 2016 were divided into two groups according to the random digit table, 44 cases in each group. The experimental group received team-based outpatient health education and the control group the routine health education. The two groups were compared in view of blood pressure and hypertension. Result The intervention group was significantly better in blood pressure and self-management ability as compared to the control group (P<0.01). Conclusion The team-based health education can help the young and middle-aged patients with good hypertension management and blood pressure control so that it improves the quality of life.

Objective To study the primiparas' sexual problems after delivery and the effect of in-dividualized nursing interventions on improvement of these problems in order to provide theoretic base-ment for sexual health education after delivery. Methods 248 primiparas after delivery in women's hos-pital were randomly divided into the intervention group and the control group with 124 cases in each group. All of them received self- designed questionnaires about sexual problems one year before pregnancy. The primiparas in two groups all receives routine care. Besides, the intervention group was given sexual health manual and received one-to-one individualized nursing intervention on sexual life after delivery according to their own conditions. The two groups were all received countercheck during 4 to 6 months after delivery and the results underwent X2 test. Results There was no significant difference between the sexual prob-lems one year before pregnancy for the two groups. But when calling back during 4 to 6 months after deliv-ery, compared with the control group, the intervention group had less sexual problems. Conclusions It is necessary to strengthen sexual health education in order to improve the sexual problems after delivery. Indi-vidualized nursing interventions can not only fulfill the sexual health demands of different primiparas after delivery, but also guarantee the women's privacy and safety.

AIM: To analyze the nucleotide and putative amino acid sequences of PIA genes isolated from N. gonorrhoeae and to construct the prokaryotic expression system of PIA gene.METHODS: The entire PIA genes from 9 strains of N. gonorrhoeae were amplified by using high fidelity PCR. The target amplification fragments were sequenced after T-A cloning. Homology comparison of the nucleotide and putative amino acid sequences of PIA genes from the isolates with the reported sequences in GenBank was then performed. A prokaryotic expression system of PIA gene was constructed. Different dosages of IPTG were applied to induce the expression of the target recombinant protein (rPIA) and 10% SDS-PAGE plus Bio-Rad Agarose Image Analysor was used to determine the expression level of rPIA. rPIA was extracted using Ni-NTA affinity chromatography and the purified effect was detected by SDS-PAGE.RESULTS: In comparison with the reported PIA gene sequences (GenBank No: L19962), the homologies of nucleotide and putative amino acid sequences of PIA genes from the isolates were 99.6%-100% and 99.1%-100%, respectively, which indicated that all the isolates were belonging to serovars IA6. Output of rPIA was as high as 50.1% of the total bacterial proteins. The purified rPIA only showed a single target protein fragment in gel.CONCLUSION: Serovar IA6 is dominant in the local N. gonorrhoeae isolates and sequences of the encoding gene are relatively conserved. The constructed prokaryotic expression system is able to express rPIA with high efficiency, which may lay a foundation for further development of serological detection kit and vaccine of N. gonorrhoeae.