Objective:To investigate associations of serum β 2-microglobulin (β 2M) with baseline stroke severity and outcome at 3 months after onset in patients with acute ischemic stroke (AIS). Methods:Patients with first-ever AIS admitted to the Department of Neurology, the Affiliated Municipal Hospital of Xuzhou Medical University from January 2018 to May 2022 were included retrospectively. The severity of stroke was evaluated according to the National Institute of Health Stroke Scale score at admission. ≤8 was defined as mild stroke, and >8 was defined as moderate to severe stroke. At 3 months after onset, the modified Rankin Scale was used to evaluate the clinical outcome, ≤2 points was defined as good outcome and >2 points was defined as poor outcome. Multivariate logistic regression analysis was used to determine associations of β 2M with the severity and outcome of stroke. Results:A total of 463 patients with AIS were enrolled, including 263 males (56.68%), aged 62.89±11.77 years. There were 319 patients (68.89%) in the mild stroke group and 144 (31.10%) in the moderate to severe stroke group; 304 patients (65.66%) in the good outcome group and 159 (34.34%) in the poor outcome group. Univariate analysis showed that the β 2M in the moderate to severe stroke group and the poor outcome group were significantly higher than those in the mild stroke group and the good outcome group, respectively (all P<0.05). Multivariate logistic regression analysis showed that β 2M was an independent influencing factor for stroke severity (odds ratio 2.476, 95% confidence interval 1.621-3.783; P<0.001) and poor outcome (odds ratio 5.179, 95% confidence interval 2.941-11.121; P<0.001) in patients with AIS. Conclusion:There is a significant independent correlation between high serum β 2M and the severity and poor outcome of stroke in patients with AIS.

This article summarizes the current application of Traditional Chinese Medicine (TCM) nursing techniques in clinical nursing of obstetrics and gynecology, providing a reference for the clinical nursing and related research of obstetric and gynecological diseases in TCM, and promoting the widespread application of TCM nursing techniques in obstetrics and gynecology.

Objective : To study the effect of ubiquinol⁃cytochrome C reductase subunit 2 (QCR2) on cell cycle arrest of cervical cancer SiHa cell line , and to explore related mechanisms. Methods : The log⁃phase SiHa cells were taken , and QCR2 siRNA and control siRNA were transfected into SiHa cells by liposome transfection , which were set as QCR2 siRNA group and control siRNA group , and untreated cells were set as a blank group. qRT⁃PCR and Western blot were used to determine the relative expression of QCR2 , p53 mRNA and protein. Propidium iodide(PI) staining was used to determine cell cycle. The cells in the QCR2 siRNA group were taken , and were intervened with a final concentration of 50 nmol/L ubiquitin⁃proteasome inhibitor PS341 as the QCR2 siRNA + PS341 group. In addition , the cells in the QCR2 siRNA group were intervened with the same amount of normal saline (NS) and set as the QCR2 siRNA + NS group. Western blot was used to determine the relative expression of p53 protein. The immunoprecipitation test was used to determine the level of p53 ubiquitination. Results : Observed under a fluorescence microscope , the transfection efficiency of QCR2 siRNA group and control siRNA group were both > 80% . Compared with the blank group and control siRNA group , the relative expression of QCR2 mRNA and protein in the QCR2 siRNA group decreased (P < 0. 05) , the proportion of G0/G1 increased (P < 0. 05) , and the proportion of S , G2/M decreased (P < 0. 05) . There was no significant difference in the relative expression of p53 mRNA between the groups. Compared with the blank group and control siRNA group , the relative expression of p53 protein in the QCR2 siRNA group increased (P < 0. 05) . Compared with the blank group and QCR2 siRNA + PS341 group , the relative expression of p53 protein in the QCR2 siRNA group and QCR2 siRNA + NS group increased (P < 0. 05) . Compared with the blank group and control siRNA group , the degree of p53 ubiquitination in the QCR2 siRNA group was reduced ( P < 0. 05 ) . Conclusion Silencing QCR2 can block SiHa cells in G0/G1 phase. Its mechanism may be related to the inhibition of p53 ubiquitination and the increase of its protein expression.

This paper summarizes the current situation and necessity of the training of traditional Chinese medicine (TCM) nursing specialists in China, as well as the existing achievements and existing problems, and puts forward the prospects and suggestions for the training of TCM nursing team, so as to strengthen the construction of TCM nursing culture, build a high-quality TCM nursing talent team, and promote the high-quality development of TCM nursing.

Objective:To analyze the clinical characteristics and prognosis of pregnant women with hemorrhagic fever with renal syndrome (HFRS).Methods:A total of 11 pregnant women with HFRS admitted to The Second Affiliated Hospital of Xi′an Jiaotong University (four cases), The Second Affiliated Hospital of Air Force Medical University (four cases), The First Affiliated Hospital of Xi′an Jiaotong University (one case) and Central Hospital of Xianyang City (two cases) between November 2009 and February 2019 were included as the study group, and 24 age-matched non-pregnant women with HFRS were selected as the control group. The age, complications, clinical classification and laboratory indexes of the two groups were analyzed retrospectively, and the clinical outcomes of pregnant women and their fetuses in the study group were followed up. The data between two groups were compared using Mann-Whitney U test or chi-square test. Results:Patients in the study and control groups were 29 (22, 33) and 32 (24, 37) years old, respectively. Seven of 11 patients in study group were severe and critical cases, which was significantly higher than that in the control group (16.7%(4/24), χ2=7.722, P=0.015). In the study group, 10 patients had hypervolemic syndrome, 10 patients had pulmonary edema and six patients had overlapping hypotension shock phase and oliguria phase, which were all higher than those in the control group ((2/24, 8.3%), (2/24, 8.3%) and (2/24, 8.3%), respectively; χ2=22.828, 22.828 and 9.135, respectively, all P<0.01). Compared with the control group, the pregnant patients in study group had a higher urea nitrogen maximum and serum creatinine maximum, and the differences were both statistically significant ( Z=-2.453 and -2.336, respectively, both P<0.05), while they had a lower serum albumin minimum, hemoglobin maximum and hemoglobin minimum, and the differences were all statistically significant ( Z=-3.742, -3.350 and -4.034, respectively, all P<0.01). All pregnant women with HFRS recovered. Nine pregnant women gave birth to nine healthy infants. All of them received breastfeeding and the feeding duration were more than six months. No abnormal growth and development were found during an average follow-up of three years. Conclusions:Pregnancy can aggravate the severity of HFRS, and pregnant women have higher risk of the multiple stages overlap and the complications such as hypervolemic syndrome and acute pulmonary edema. After recovery from HFRS, mother may carry to full-term pregnancy.

Follicular lymphoma (FL) is usually occult, with a wide range of disease manifestations and prognosis. In recent years, with the combination of anti-CD20 monoclonal antibody and standard chemotherapy methods, as well as the use of immunomodulators and hematopoietic stem cell transplantation, the survival rate of FL patients has been greatly improved, but it is still considered to be an incurable disease. Recent studies have shown that the chimeric antigen receptor T-cell (CAR-T) therapy can improve the survival rate and the prognosis of FL patients. The article will elaborate on the mechanism, clinical research progress, adverse reactions and limitations of CAR-T in the treatment of FL.

Primary myelofibrosis (PMF) is a type of myeloproliferative neoplasms (MPN) with the worst prognosis in MPN. Immunomodulators, erythropoiesis-stimulating agents, cytoreductive drugs, splenectomy, etc. can significantly reduce the symptoms of patients, but can not improve the prognosis of patients. Allogeneic hematopoietic stem cell transplantation remains the only method that may cure PMF. The marketed JAK kinase inhibitor ruxolitinib and the hypomethylating agents, anti-fibrosis agents and telomerase inhibitorprovide new ideas for the treatment of PMF, made it possible to reverse the natural course of myelofibrosis. This paper reviews the progress of PMF therapy.

Objective To explore a new method for synthesizing arginine modified chitosan ( AC ) with mono-arginine substitution and high degree of substitution, and to evaluate the biological effect of AC as gene carriers. Methods The single arginine modified chitosan (sAC) was synthesized by means of protecting and de-protecting the arginine amino group before and after chitosan modified arginine reaction. Liu's arginine-modified chitosan ( LAC ) was prepared according to the methods reported in the literature . The conjunction of arginine to chitosan was detected by infrared spectroscopy and nuclear magnetic resonance spectroscopy. Three kinds of chitosan gene nanoparticles were respectively prepared by complex coacervation and characterized, including sAC gene nanoparticles (sACGNs), the LAC gene nanoparticles (LACGNs) and the chitosan gene nanoparticles (CGNs). A10 rat vascular smooth cells transfected with sACGNs were used to estimate the in vitro cellular uptake, internalization mechanisms and transfection efficiency. Thiazolyl blue tetrazolium blue (MTT) assay was used to measure the cytotoxicity. Results The infrared spectrum analysis confirmed that sAC was obtained via the conjunction of arginine to chitosan. Nuclear magnetic resonance spectrum analysis showed that the degree of substitution of arginine in sAC and LAC was 21.3％and 6.4％, respectively. When the ratio of nitrogen to phosphorus (N/P ratio) was 2:1, the particle sizes of CGN, LACGN, and sACGN were (94.81±2.93) nm, (124.53±2.55) nm, and (128.53±2.04) nm, respectively, and the Zeta potentials were (3.50±1.61) mV, (5.74±0.41) mV, and (6.04±1.39) mV, respectively. For the cellular uptake, CGNs were mainly through the clathrin-mediated endocytic pathway, and LACGNs and sACGN were mainly through the caveolin-mediated endocytic pathway. Compared with CGNs, LACGNs and sACGNs showed higher cellular uptake and transfection efficiency , and the differences were statistically significant ( all P<0 . 05 ) . sACGNs achieved the highest transfection efficiency in the near-neutral pH environment. MTT results showed that when the mass concentration of sACGNs reached 100μg/ml, the survival rate of A10 cells was still higher than 90％, indicating the non-cytotoxicity of sACGNs. Conclusion The new method successfully synthesized single arginine-modified chitosan. As a gene carrier, sACGNs show higher gene transfection efficiency and lower cytotoxicity than CGNs and LACGNs in near neutral pH environment.

Objective To explore the clinical effect of three unions of traditional Chinese medicine (TCM) nursing technology (auricular plaster therapy,TCM foot bath and TCM pillow) in treating hypertension (HTN) and improving life quality of patients in cardiology department. Methods A total of 95 HTN patients who were eligible for the inclusion criteria in cardiology department of Affiliated Dongfang Hospital of Beijing University of Chinese Medicine were recruited from January 2015 to January 2016. All of the research subjects were randomly divided into control group (n=53) and treatment group (n=42) according to the random number table. Conventional nursing was given to both group, and on the basis of this, the treatment group was treated with auricular plaster therapy, TCM foot bath and TCM pillow combined intervention. The anti-hypertensive effects and quality of life improvement were monitored and compared. Results The effectiveness rate of anti-hypertension of the treatment group was 85.7%, by contrast, it was 67.9% in the control group, and the difference was statistically significant (P< 0.05). There were no statistical differences in physical function and physical role between the two groups (P> 0.05). Vitality difference of the treatment group is higher than the control group, with a statistical difference (P< 0.05). Conclusions The three unions of TCM nursing technology can enhance the anti-hypertensive effect, improve the quality of life, especially improve patients' vitality significantly.

Objective To describe the clinical application of nutritional risk screening in tuberculosis department nurses, and explore the related factors that affect the clinical application of nutritional risk screening. Methods Totally 110 tuberculosis nurses were investigated about the clinical application of nutritional risk screening by self-design questionnaire. The questionnaire content totally included 20 items:general information, knowledge of nutritional risk screening ( 5 questions ) , the degree of attention to nutritional risk screening (5 questions), clinical development situation of nutritional support (5 questions) and clinical application of nutritional risk screening (5 questions).Results The nutritional risk screening score of tuberculosis department nurse was (13.200±2.841). Multiple linear regression analysis found that education background, attention to nutritional risk screening, clinical development situation of nutritional support were related factors that affect the nutritional risk screening score of tuberculosis nurses. Conclusions We should improve the ability of tuberculosis department nurse in nutritional risk screening and the consciousness of nutritional risk screening, and increase the clinical application of nutritional risk screening of tuberculosis department nurses.