Objective To explore the effects of 1 800 MHz electromagnetic radiation(EMR)on cognitive function of 3xTg-AD and 57C mice,providing a theoretical basis for the potential impacts of electromagnetic radiation on the human body.Methods A total of 12 3xTg-AD transgenic mice and 12 wild-type C57 mice were selected as research subjects.The one-month-old mice were divided into four groups:RF WT,Control WT,RF AD,and Control AD,with 6 mice in each group.The 1 800 MHz EMR exposure experiments were conducted from 20:00 to 8:00 the next day for a duration of 5 months.After the exposure,a water maze test was conducted to evaluate the effects of EMR on spatial learning and memory abilities of 3xTg AD mice,along with measurements of body weight,brain weight,and calculation of the brain-to-body ratio.Finally,Western Blot technique was used to measure the levels of APP,NR1,and NR2A in hippocampal tissue to analyze effects of 1 800 MHz EMR on the cognitive function of 3xTg AD mice.Results Under 1 800 MHz EMR exposure,there were no statistically significant differences in Morris water maze spatial learning ability among the four groups(P>0.05).However,longer escape time,greater swimming distances,and more crossings of target quadrant were exhibited in the RF AD group compared to the other groups(P<0.05).Western Blot results showed that the APP protein levels in 3xTg AD mice was higher than those in C57 mice(P<0.05).The expression levels of NR1 protein in the WT group was higher than those in the AD group(P<0.05);in the AD group,the RF AD group had higher levels than the Control WT group(P<0.05),and the NR2A protein levels in the Control WT group were higher than in the other groups(P<0.05).Conclusion Prolonged exposure to 1 800 MHz EMR can affect the learning and cognitive function of both 3xTg AD and C57 mice.

OBJECTIVE To analyze the dose-adjusted concentrations of Posaconazole oral suspension in patients undergoing hematopoietic stem cell transplantation （HSCT） and their influential factors. METHODS Data were collected from hospitalized HSCT patients admitted to the First Affiliated Hospital of Shandong First Medical University （Shandong Provincial Qianfoshan Hospital） from January 2021 to April whtwhm@yeah.net 2023 who took Posaconazole oral suspension for the prevention of invasive fungal disease （IFD） and received blood concentration of posaconazole. The rate of concentration attainment and clinical failure rate of posaconazole for the prevention of IFD were evaluated， and one-way and multiple linear regression analyses were performed for the influential factors of dose-adjusted concentrations （C0/D） of posaconazole. RESULTS A total of 44 patients were enrolled； the mean C0 of posaconazole in patients was （0.99±0.94） µg/mL， and 20 patients had a C0≥0.7 μg/mL， with a concentration attainment rate of 45.45% for the prevention of IFD； 13 cases were clinical failures， with a clinical failure rate of 29.55%. Of 24 patients who did not achieve C0/D of posaconazole for IFD prophylaxis， one patient was a clinical failure despite timely dose adjustment of posaconazole in seven patients； seven of the thirteen patients who did not undergo dose adjustment were clinical failures； and the remaining four patients were switched to other antifungal agents. The results of univariate analysis showed that gender， body mass index （BMI）， renal function， combined use of sodium phenytoin， omeprazole and metoclopramide had a significant effect on the C0/D of posaconazole （P＜0.05）； the results of multivariate linear regression analysis showed that gender， BMI and combined use of sodium phenytoin were the independent factors affecting the C0/D of posaconazole （P＜0.05）. CONCLUSIONS Significant individual differences are reflected in the blood concentration of Posaconazole oral suspension； gender， BMI and combined use of sodium phenytoin are independent factors affecting the C0/D of posaconazole.

Objective:To investigate the clinical value of internal thoracic artery perforator propeller flap combines with latissimus dorsi kiss flap in repairing large skin defect of chest wall after breast tumor operation.Methods:A retrospective analysis was made on the clinical data from 6 cases of breast tumors admitted to the First Affiliated Hospital of Dalian Medical University between January 2018 and May 2019. There were 6 females with an average age of 55.5 years (range, 46-73 years); five of them were all locally advanced breast cancer, one of them was advanced breast cancer. The median disease duration is 20.1 months (range, 4 months to 5 years). Four of them accepted chemotherapy before surgery. The area of skin defect ranged from 10 cm× 15 cm to 21 cm× 31 cm after their primary tumor resection; the internal thoracic artery perforator propeller flap were designed to repair wounds primarily or combined with pedicled latissimus dorsi kiss flap. One of the patients had a large contralateral breast and underwent breast reduction and reconstruction simultaneously. Postoperative follow-up was conducted to observe the flap status and tumor recurrence.Results:In the six cases, the size of internal thoracic artery perforator propeller flap was 5 cm× 12 cm to 10 cm× 23 cm. Three cases combined with kiss flap, with a size of 5 cm× 15 cm to 7 cm× 18 cm for each lobe. The flap of six patients survived successfully. Five flaps survived with primary healing of wound; one patient suffered from subcutaneous effusion due to a slightly larger tension in the donor area of the back, and delayed wound healing after dressing change and drainage. The follow-up time was from 1 to 17 months (mean, 7.5 months) after surgery. The flap had good appearance. The shoulder joint and lumbar activities were normal. No local recurrence occurred and no obvious scar were found at donor sites.Conclusions:The application of internal thoracic artery perforator propeller flap and its combined flap in repairing chest wall huge skin defects after breast tumor resection has great clinical value, because no need of vessel anastomosis. It is simple, reliable and rapid postoperative recovery with few complication, and can achieve satisfactory result.

Objective:To investigate the clinical value of internal thoracic artery perforator propeller flap combines with latissimus dorsi kiss flap in repairing large skin defect of chest wall after breast tumor operation.Methods:A retrospective analysis was made on the clinical data from 6 cases of breast tumors admitted to the First Affiliated Hospital of Dalian Medical University between January 2018 and May 2019. There were 6 females with an average age of 55.5 years (range, 46-73 years); five of them were all locally advanced breast cancer, one of them was advanced breast cancer. The median disease duration is 20.1 months (range, 4 months to 5 years). Four of them accepted chemotherapy before surgery. The area of skin defect ranged from 10 cm× 15 cm to 21 cm× 31 cm after their primary tumor resection; the internal thoracic artery perforator propeller flap were designed to repair wounds primarily or combined with pedicled latissimus dorsi kiss flap. One of the patients had a large contralateral breast and underwent breast reduction and reconstruction simultaneously. Postoperative follow-up was conducted to observe the flap status and tumor recurrence.Results:In the six cases, the size of internal thoracic artery perforator propeller flap was 5 cm× 12 cm to 10 cm× 23 cm. Three cases combined with kiss flap, with a size of 5 cm× 15 cm to 7 cm× 18 cm for each lobe. The flap of six patients survived successfully. Five flaps survived with primary healing of wound; one patient suffered from subcutaneous effusion due to a slightly larger tension in the donor area of the back, and delayed wound healing after dressing change and drainage. The follow-up time was from 1 to 17 months (mean, 7.5 months) after surgery. The flap had good appearance. The shoulder joint and lumbar activities were normal. No local recurrence occurred and no obvious scar were found at donor sites.Conclusions:The application of internal thoracic artery perforator propeller flap and its combined flap in repairing chest wall huge skin defects after breast tumor resection has great clinical value, because no need of vessel anastomosis. It is simple, reliable and rapid postoperative recovery with few complication, and can achieve satisfactory result.