Objective To investigate the possible mechanism involved in the effect of micro RNA-10a(miRNA) on hepatic fibrosis (HF),through the observation on the expression of TGFβ1/Smads signal transduction pathway in mice with HF and its correlation with the development of hepatic fibrosis.Methods Forty healthy male mice with an age of 9 weeks (C57BL6/J) were randomly divided into the control group and the HF model group (or the observation group).The animals in the control group were given normal saline abdominally at a dosage of 5 μl/g,twice a week,for a succession of 8 weeks,while the animals in the observation group received 10％ CCL4 olive oil also at a dosage of 5 μl/g with the same frequency and for the same length of time to develop the model of hepatic fibrosis.The expression of miRNA-10a was detected by RT-PCR.HF cells were cultured in the animals of the observation group and miRNA-10a mimics were transfected (the transfection group).CCK-8 method was used to detect the proliferation of HF cells,and the expression levels of TGFβ1 and Smad 7 in the HF cells were detected by Western-blotting.Results As compared with that of the control group,the expression level of miRNA-10a in the observation group was increased significantly (P ＜ 0.05);and as compared with that of the control group,the expression level of miRNA-10a in the transfection group were significantly decreased (P ＜ 0.05);and furthermore,as compared with that of the control group,the lower expression of miRNA-10a significantly decreased the expression level of TGFβ1 and increased the expression level of Smad 7 (P ＜ 0.05).Conclusion The expression of miRNA-10a in the HF cells was lower,and the transfected miRNA-10a mimics could significantly promote the proliferation of HF cells,the possible mechanism of which might be associated with the regulation of TGFβ1/Smads signal transduction pathway in HF promotion.

Objective To study clinical features of obstructive sleep apnea syndrome (OSAS) in senile patients.Methods Forty cases of OSAS that were diagnosed and treated in the pulmonary department of our hospital from Mar.2012 to Aug.2013 were chosen as the observation group, and another 40 healthy adults were assigned as the control group.The levels of leptin, C reactive pro-tein ( CRP) and basic diseases in the 80 study subjects were closely observed, and then, clinical features of the patients with OSAS were analyzed.Results Compared with the control group, the patients in the observation group mostly had such primary diseases as o-besity, cardiovascular and cerebrovascular diseases, and the levels of leptin and CRP were significantly higher than those of the control group, with statistical significance (P<0.05 or <0.01).Conclusion Serum leptin and CRP levels in the OSAS patients could rea-sonably represent the seriousness of the disease, and they might have synergistic effects in the development of OSAS.