Objective:To explore the inhibitory effect of fentanyl combined with hyperbaric oxygen (HBO) on the proliferation of tumor tissues of the gastric cancer tissues of nude mice.Methods:A total of 20 six-week-old male specific pathogen free (SPF) BALB/c nude mice were used to establish a model of transplanted tumors and then were divided into four groups according to the experimental requirements: control group, HBO group, FEN group, and HBO+ FEN group. The control group was raised normally after successful modeling; the HBO group inhaled oxygen for 60 min every day at 0.20 MPa; the FEN group was given 10 mg/kg fentanyl by intragastric administration; and the HBO+ FEN group inhaled oxygen for 60 min at first at 0.20 MPa and then was given 10 mg/kg fentanyl by intragastric administration. All groups were treated every other day for a total of 21 days. After treatment, the nude mice were executed to strip the tumor of which the volume and weight were measured. Terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay was applied to detect the apoptosis of tumor cells and Western blotting was adopted to detect the expressions of apoptosis-related proteins and signaling pathway protein.Results:After 21 days of treatment, the tumor volume and weight of the mice in the HBO+ FEN group were significantly lower than those of the other three groups with statistically significant differences ( P<0.05). The number of apoptotic cells of transplanted tumors in the HBO+ FEN group was significantly higher than those of the other three groups with statistically significant differences ( P<0.05). Compared with the control group, the FEN group, and the HBO group, the expression levels of Bax, Caspase-3, and Cytochrome C in transplanted tumor tissues of nude mice in the HBO+ FEN group were significantly higher, while the expression of Bcl-2 protein was significantly lower, with statistically significant differences ( P<0.05). Compared with the control group, the HBO group, and the FEN group, the expression level of p65 protein in transplanted tumor cells in the HBO+ FEN group was significantly lower, while the expression level of p53 protein was significantly higher, with statistically significant differences ( P<0.05). Conclusion:The combination of HBO and fentanyl can promote tumor cell apoptosis by regulating NF-κB and p53 signaling pathways in nude mice with gastric cancer.

Objective:To explore the inhibitory effect of fentanyl combined with hyperbaric oxygen (HBO) on the proliferation of tumor tissues of the gastric cancer tissues of nude mice.Methods:A total of 20 six-week-old male specific pathogen free (SPF) BALB/c nude mice were used to establish a model of transplanted tumors and then were divided into four groups according to the experimental requirements: control group, HBO group, FEN group, and HBO+ FEN group. The control group was raised normally after successful modeling; the HBO group inhaled oxygen for 60 min every day at 0.20 MPa; the FEN group was given 10 mg/kg fentanyl by intragastric administration; and the HBO+ FEN group inhaled oxygen for 60 min at first at 0.20 MPa and then was given 10 mg/kg fentanyl by intragastric administration. All groups were treated every other day for a total of 21 days. After treatment, the nude mice were executed to strip the tumor of which the volume and weight were measured. Terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay was applied to detect the apoptosis of tumor cells and Western blotting was adopted to detect the expressions of apoptosis-related proteins and signaling pathway protein.Results:After 21 days of treatment, the tumor volume and weight of the mice in the HBO+ FEN group were significantly lower than those of the other three groups with statistically significant differences ( P<0.05). The number of apoptotic cells of transplanted tumors in the HBO+ FEN group was significantly higher than those of the other three groups with statistically significant differences ( P<0.05). Compared with the control group, the FEN group, and the HBO group, the expression levels of Bax, Caspase-3, and Cytochrome C in transplanted tumor tissues of nude mice in the HBO+ FEN group were significantly higher, while the expression of Bcl-2 protein was significantly lower, with statistically significant differences ( P<0.05). Compared with the control group, the HBO group, and the FEN group, the expression level of p65 protein in transplanted tumor cells in the HBO+ FEN group was significantly lower, while the expression level of p53 protein was significantly higher, with statistically significant differences ( P<0.05). Conclusion:The combination of HBO and fentanyl can promote tumor cell apoptosis by regulating NF-κB and p53 signaling pathways in nude mice with gastric cancer.

Objective:To investigate the clinical efficacy and mechanism of ozagrel sodium and vinpocetine combined with hyperbaric oxygen (HBO) in the treatment of acute cerebral infarction (ACI).Methods:A total of 172 patients with ACI, admitted to the Department of Neurology, Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from December 2016 to June 2019, were selected and divided into groups A, B, C, and D by lottery with the ratio of 1∶1∶1∶1, i. e. 43 cases in each group. The group A was treated with ozagrel sodium, the group B was treated with vinpocetine, the group C was treated with vinpocetine and ozagrel sodium, and the group D was treated with vinpocetine, ozagrel sodium, and HBO. The clinical effects of the four groups were observed, and the National Institutes of Health Stroke Scale (NIHSS) and the Montreal Cognitive Assessment (MoCA) scores were compared. The levels of serum oxidative stress factors, i. e. malondialdehyde (MDA) and superoxide dismutase (SOD), the levels of brain injury response factors, i. e. macrophage colony stimulatory factor (M-CSF) and soluble intercellular adhesion molecule-1 (sICAM-1), and the level of vascular endothelial growth factor were measured by enzyme linked immunosorbent assay.Results:After 4 courses of treatment, the total effective rate of the group D (93.0%) was significantly higher than that of the group C (83.7%), and the total effective rate of the group C (83.7%) was significantly higher than those of the group B (74.4%) and the group A (72.1%) ( P<0.05). The NIHSS score of the group D was significantly lower than that of the group C, and the MoCA score of the group D was higher than that of the group C; the NIHSS score of the group C was significantly lower than those of the group A and the group B, and the MoCA score of the group C was higher than those of the group A and the group B, the differences were statistically significant ( P<0.05). The serum MDA level of the group D was significantly lower than that of the group C, and the SOD level of the group D was higher than that of the group C; the serum MDA level of the group C was significantly lower than those of the group A and the group B, and the SOD level of the group C was higher than those of the group A and the group B, the differences were statistically significant ( P<0.05). The serum levels of sICAM-1 and M-CSF in the group D were significantly lower than those in the group C, and the serum levels of sICAM-1 and M-CSF in the group C were significantly lower than those in the group A and the group B, with statistically significant differences ( P<0.05). The serum levels of BDNF and VEGF in the group D were significantly higher than those in the group C, and the serum levels of BDNF and VEGF in the group C were significantly higher than that in group A and the group B, with statistically significant differences ( P<0.05). Conclusion:Ozagrel sodium and vinpocetine combined with HBO can significantly improve the curative effect on ACI mainly by regulating the levels of brain injury response factors, oxidative stress factors, and neurovascular growth factor after ACI.

Objective:To investigate the clinical effect and action mechanism of Smecta and Kangfuxin mixture retention enema combined with hyperbaric oxygen (HBO) in the prevention of radiation proctitis (RP) after radiotherapy for cervical cancer.Methods:A total of 92 patients with RP after radiotherapy for cervical cancer admitted to Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2016 to December 2019 were selected and divided into observation group and control group according to their treatments, with 46 cases each. The control group was treated with Smecta and Kangfuxin mixture retention enema, and the observation group was treated with HBO on the basis of the treatment of the control group. The incidence, time of occurrence, and classification of acute and chronic RP of both groups were recorded. The results of colonoscopy were observed and evaluated by the Viennese colonoscopy score. The levels of immunoglobulin IgG, IgM, and IgA in the two groups before and after treatment were detected by immunoturbidimetric method. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of C-reactive protein (CRP) was detected by fluorescence method.Results:The incidence of acute RP after radiotherapy in the observation group was significantly lower than that in the control group, and the average occurrence time of RP in the observation group was significantly delayed than that in the control group ( P<0.05). The incidences of grade 1-2, grade 3-4 RP, and the total incidence of acute and chronic RP in the observation group were significantly lower than those in the control group ( P<0.05). The patients with 0-2 points of Viennese colonoscopy scores in the observation group were significantly more than those in the control group, and the patients with 3-5 points were less than those in the control group, the differences were statistically significant ( P<0.05). After treatment, the levels of IgG, IgM, and IgA in the observation group were significantly higher than those in the control group, while the levels of TNF-α, IL-6, and CRP in the observation group were significantly lower than those in the control group ( P<0.05). Conclusion:Smecta and Kangfuxin mixture retention enema combined with Hyperbaric oxygen (HBO) can delay and reduce the occurrence of radiation proctitis and relieve the clinical symptoms of cervical cancer patients after radiotherapy. The mechanism may be achieved by improving the immune function and reducing the level of inflammatory factors.

Objective:To investigate the clinical efficacy and mechanism of ozagrel sodium and vinpocetine combined with hyperbaric oxygen (HBO) in the treatment of acute cerebral infarction (ACI).Methods:A total of 172 patients with ACI, admitted to the Department of Neurology, Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from December 2016 to June 2019, were selected and divided into groups A, B, C, and D by lottery with the ratio of 1∶1∶1∶1, i. e. 43 cases in each group. The group A was treated with ozagrel sodium, the group B was treated with vinpocetine, the group C was treated with vinpocetine and ozagrel sodium, and the group D was treated with vinpocetine, ozagrel sodium, and HBO. The clinical effects of the four groups were observed, and the National Institutes of Health Stroke Scale (NIHSS) and the Montreal Cognitive Assessment (MoCA) scores were compared. The levels of serum oxidative stress factors, i. e. malondialdehyde (MDA) and superoxide dismutase (SOD), the levels of brain injury response factors, i. e. macrophage colony stimulatory factor (M-CSF) and soluble intercellular adhesion molecule-1 (sICAM-1), and the level of vascular endothelial growth factor were measured by enzyme linked immunosorbent assay.Results:After 4 courses of treatment, the total effective rate of the group D (93.0%) was significantly higher than that of the group C (83.7%), and the total effective rate of the group C (83.7%) was significantly higher than those of the group B (74.4%) and the group A (72.1%) ( P<0.05). The NIHSS score of the group D was significantly lower than that of the group C, and the MoCA score of the group D was higher than that of the group C; the NIHSS score of the group C was significantly lower than those of the group A and the group B, and the MoCA score of the group C was higher than those of the group A and the group B, the differences were statistically significant ( P<0.05). The serum MDA level of the group D was significantly lower than that of the group C, and the SOD level of the group D was higher than that of the group C; the serum MDA level of the group C was significantly lower than those of the group A and the group B, and the SOD level of the group C was higher than those of the group A and the group B, the differences were statistically significant ( P<0.05). The serum levels of sICAM-1 and M-CSF in the group D were significantly lower than those in the group C, and the serum levels of sICAM-1 and M-CSF in the group C were significantly lower than those in the group A and the group B, with statistically significant differences ( P<0.05). The serum levels of BDNF and VEGF in the group D were significantly higher than those in the group C, and the serum levels of BDNF and VEGF in the group C were significantly higher than that in group A and the group B, with statistically significant differences ( P<0.05). Conclusion:Ozagrel sodium and vinpocetine combined with HBO can significantly improve the curative effect on ACI mainly by regulating the levels of brain injury response factors, oxidative stress factors, and neurovascular growth factor after ACI.

Objective:To investigate the clinical effect and action mechanism of Smecta and Kangfuxin mixture retention enema combined with hyperbaric oxygen (HBO) in the prevention of radiation proctitis (RP) after radiotherapy for cervical cancer.Methods:A total of 92 patients with RP after radiotherapy for cervical cancer admitted to Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2016 to December 2019 were selected and divided into observation group and control group according to their treatments, with 46 cases each. The control group was treated with Smecta and Kangfuxin mixture retention enema, and the observation group was treated with HBO on the basis of the treatment of the control group. The incidence, time of occurrence, and classification of acute and chronic RP of both groups were recorded. The results of colonoscopy were observed and evaluated by the Viennese colonoscopy score. The levels of immunoglobulin IgG, IgM, and IgA in the two groups before and after treatment were detected by immunoturbidimetric method. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of C-reactive protein (CRP) was detected by fluorescence method.Results:The incidence of acute RP after radiotherapy in the observation group was significantly lower than that in the control group, and the average occurrence time of RP in the observation group was significantly delayed than that in the control group ( P<0.05). The incidences of grade 1-2, grade 3-4 RP, and the total incidence of acute and chronic RP in the observation group were significantly lower than those in the control group ( P<0.05). The patients with 0-2 points of Viennese colonoscopy scores in the observation group were significantly more than those in the control group, and the patients with 3-5 points were less than those in the control group, the differences were statistically significant ( P<0.05). After treatment, the levels of IgG, IgM, and IgA in the observation group were significantly higher than those in the control group, while the levels of TNF-α, IL-6, and CRP in the observation group were significantly lower than those in the control group ( P<0.05). Conclusion:Smecta and Kangfuxin mixture retention enema combined with Hyperbaric oxygen (HBO) can delay and reduce the occurrence of radiation proctitis and relieve the clinical symptoms of cervical cancer patients after radiotherapy. The mechanism may be achieved by improving the immune function and reducing the level of inflammatory factors.

Objective To explore the clinical effects of adjuvant hyperbaric oxygen ( HBO) therapy on peritoneal metastasis of gastric cancer. Methods A retrospective analysis was made on the 88 patients with peritoneal metastasis of gastric cancer admitted to the hospital from August 2014 to August 2016. The patients were divided into the observation group ( n =39 ) and the control group ( n =49 ) by different treatment methods. The control group was treated with chemotherapy, while the observation group received adjuvant HBO therapy in addition to the treatment received by the control group. After 2 courses of treatment, the short-term efficacy and acute and sub-acute toxicity were compared between the patients of the 2 groups, and the life quality of the patients was evaluated by using QLQ-C30. One-year medical follow up was made, and disease progression and survival time were compared between the patients of the 2 groups. Results The objective response rate (ORR) of the observation group and the control group were 53. 85％ and 46. 94％, respectively. There was no statistical difference, when comparison was made between the 2 groups(χ2 =0. 414, P=0. 520). The digestive tract and blood toxicity scores of the observation group were (1. 08 ± 0. 26)and (1. 01 ± 0. 22) respectively, which were significantly lower than (3. 26 ± 0. 62) and (1. 67 ± 0. 41) of the control group (P<0. 05). The median disease progression time of the observation group and the control group was respectively 6 months and 3 months, and the median survival time was respectively 8. 9 months and 5. 1 months, with the observation group all being superior to those of the control group ( P < 0. 05 ). The comprehensive scores of the life quality and physical function in the observation group were higher than those in the control group (P<0. 05). Conclusion HBO adjuvant therapy for gastric cancer patients with peritoneal metastasis has good short-term efficacy, and could slow disease progress, improve their life quality, and more importantly showed less adverse reactions.

Objective To explore the clinical effects of adjuvant hyperbaric oxygen ( HBO) therapy on peritoneal metastasis of gastric cancer. Methods A retrospective analysis was made on the 88 patients with peritoneal metastasis of gastric cancer admitted to the hospital from August 2014 to August 2016. The patients were divided into the observation group ( n =39 ) and the control group ( n =49 ) by different treatment methods. The control group was treated with chemotherapy, while the observation group received adjuvant HBO therapy in addition to the treatment received by the control group. After 2 courses of treatment, the short-term efficacy and acute and sub-acute toxicity were compared between the patients of the 2 groups, and the life quality of the patients was evaluated by using QLQ-C30. One-year medical follow up was made, and disease progression and survival time were compared between the patients of the 2 groups. Results The objective response rate (ORR) of the observation group and the control group were 53. 85％ and 46. 94％, respectively. There was no statistical difference, when comparison was made between the 2 groups(χ2 =0. 414, P=0. 520). The digestive tract and blood toxicity scores of the observation group were (1. 08 ± 0. 26)and (1. 01 ± 0. 22) respectively, which were significantly lower than (3. 26 ± 0. 62) and (1. 67 ± 0. 41) of the control group (P<0. 05). The median disease progression time of the observation group and the control group was respectively 6 months and 3 months, and the median survival time was respectively 8. 9 months and 5. 1 months, with the observation group all being superior to those of the control group ( P < 0. 05 ). The comprehensive scores of the life quality and physical function in the observation group were higher than those in the control group (P<0. 05). Conclusion HBO adjuvant therapy for gastric cancer patients with peritoneal metastasis has good short-term efficacy, and could slow disease progress, improve their life quality, and more importantly showed less adverse reactions.

Objective To investigate the clinical effects of mesalazine combined with hyperbaric oxygen (HBO) on ulcerative colitis (UC) and the changes in serum immunological indexes.Methods A retrospective analysis was made of the 120 UC patients admitted to the Department of Gastroenterology,Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiaotong University from January 2014 to January 2018.The patients were randomly divided into the observation group and the control group,each consisting of 60 patients.The control group was treated with mesalazine enteric-coated tablets on the basis of routine treatment,while the observation group was supplemented with HBO therapy in addition to the treatment received by the control group.After 3 courses of treatment,clinical effective rate and UC activity indexes were compared between the 2 groups.The serum levels of tumor necrosis factor-a (TNF-a),interleukins-2,4,6 and 10 were detected by enzyme-linked immunosorbent assay (ELISA),the serum level of superoxide dismutase (SOD) was detected by nitrite method and that of malondialdehyde (MAD) was assayed by thiobarbituric acid colorimetty,the levels of immunoglobulins (IgA,IgG,IgM) were determined by agar immunoassay,and the level of asymmetric dimethylarginine (ADMA) was detected by fluorescence derivation and capillary electrophoresis.The above indicators were evaluated upon completion of the treatment.Results The total effective rate of the observation group (93.3％) was significantly higher than that of the control group (76.6％) (P ＜ 0.05),and the UC activity scores of the observation group were significantly lower than those of the control group (P ＜ 0.01).After treatment,statistical significance could be seen in the levels of serum IgA and IgG in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜ 0.05).Statistical significance could also be noticed in the levels of serum IL-2,IL-6 and IL-10 in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜0.05 or P ＜0.01).After treatment,there was also statistical significance in the serum levels of SOD and MAD in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜0.05 or P ＜ 0.01).Following treatment,statistical significance could be noted in the serum levels of TNF-a and ADMA in the 2 groups,as compared with those before treatment and those of the control group after treatment (P ＜ 0.05 or P ＜ 0.01).Conclusion Mesalazine combined with HBO could alleviate colonic tissue injury,inhibit mucosal immune response,reduce oxygen free radical level,accelerate intestinal mucosal repair and improve clinical efficacy in UC patients.For this reason,it is worth further clinical promotion and application.

Objective To investigate the clinical effects of mesalazine combined with hyperbaric oxygen (HBO) on ulcerative colitis (UC) and the changes in serum immunological indexes.Methods A retrospective analysis was made of the 120 UC patients admitted to the Department of Gastroenterology,Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiaotong University from January 2014 to January 2018.The patients were randomly divided into the observation group and the control group,each consisting of 60 patients.The control group was treated with mesalazine enteric-coated tablets on the basis of routine treatment,while the observation group was supplemented with HBO therapy in addition to the treatment received by the control group.After 3 courses of treatment,clinical effective rate and UC activity indexes were compared between the 2 groups.The serum levels of tumor necrosis factor-a (TNF-a),interleukins-2,4,6 and 10 were detected by enzyme-linked immunosorbent assay (ELISA),the serum level of superoxide dismutase (SOD) was detected by nitrite method and that of malondialdehyde (MAD) was assayed by thiobarbituric acid colorimetty,the levels of immunoglobulins (IgA,IgG,IgM) were determined by agar immunoassay,and the level of asymmetric dimethylarginine (ADMA) was detected by fluorescence derivation and capillary electrophoresis.The above indicators were evaluated upon completion of the treatment.Results The total effective rate of the observation group (93.3％) was significantly higher than that of the control group (76.6％) (P ＜ 0.05),and the UC activity scores of the observation group were significantly lower than those of the control group (P ＜ 0.01).After treatment,statistical significance could be seen in the levels of serum IgA and IgG in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜ 0.05).Statistical significance could also be noticed in the levels of serum IL-2,IL-6 and IL-10 in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜0.05 or P ＜0.01).After treatment,there was also statistical significance in the serum levels of SOD and MAD in the observation group,as compared with those before treatment and those of the control group after treatment (P ＜0.05 or P ＜ 0.01).Following treatment,statistical significance could be noted in the serum levels of TNF-a and ADMA in the 2 groups,as compared with those before treatment and those of the control group after treatment (P ＜ 0.05 or P ＜ 0.01).Conclusion Mesalazine combined with HBO could alleviate colonic tissue injury,inhibit mucosal immune response,reduce oxygen free radical level,accelerate intestinal mucosal repair and improve clinical efficacy in UC patients.For this reason,it is worth further clinical promotion and application.