OBJECTIVES: To investigate the effects of quercetin on cuproptosis and L-type calcium currents in the myocardium of diabetic rats. METHODS: Forty SD rats were randomized into control group and diabetic model groups. The rat models of diabetes mellitus (DM) induced by high-fat and high-sugar diet combined with streptozotocin (STZ) injection were further divided into DM model group, quercetin treatment group, and empagliflozin treatment group (n=10). Blood glucose and body weight were measured every other week, and cardiac function of the rats was evaluated using echocardiography. HE staining, Sirius red staining, and wheat germ agglutinin (WGA) analysis were used to observe the changes in myocardial histomorphology, and serum copper levels and myocardial FDX1 expression were detected. In cultured rat cardiomyocyte H9c2 cells with high-glucose exposure, the effects of quercetin and elesclomol, alone or in combination, on intracellular CK-MB and LDH levels and FDX1 expression were assessed, and the changes in L-type calcium currents were analyzed using patch-clamp technique. RESULTS: The diabetic rats exhibited elevated blood glucose, reduced body weight, impaired left ventricular function, increased serum copper levels and myocardial FDX1 expression, decreased L-type calcium currents, and prolonged action potential duration. Quercetin and empagliflozin treatment significantly lowered blood glucose, improved body weight, and restored cardiac function of the diabetic rats, and compared with empagliflozin, quercetin more effectively reduced serum copper levels, downregulated FDX1 expression, and enhanced myocardial L-type calcium currents in diabetic rats. In H9c2 cells, high glucose exposure significantly increased myocardial expressions of FDX1, CK-MB and LDH, which were effectively lowered by quercetin treatment; Elesclomol further elevated FDX1, CK-MB and LDH levels in the exposed cells, and these changes were not significantly affected by the application of quercetin. CONCLUSIONS Quercetin ameliorates myocardial injury in diabetic rats possibly by suppressing myocardial cuproptosis signaling and restoring L-type calcium channel activity.
Animals ; Quercetin/pharmacology* ; Calcium Channels, L-Type/metabolism* ; Diabetes Mellitus, Experimental/metabolism* ; Rats, Sprague-Dawley ; Rats ; Myocytes, Cardiac/drug effects* ; Myocardium/pathology* ; Male

Pancreatic fistula, a common complication after pancreaticoduodenectomy, is a severe condition with a high mortality. Therefore, early diagnosis of this condition and effective prevention and treatment measures are essential. This article reviews the diagnostic criteria for pancreatic fistula, the risk factors for its development, and research advances in the prevention and treatment of pancreatic fistula.

In order to improve image de-noising effect,a new threshold function de-noising method based on wavelet analysis was proposed, which can overcome the continuity problem of the hard-threshold function, and eliminate the constant deviation of the soft one by constructing a new threshold function. Experimental results showed that the new threshold function could obtain higher peak signal to noise ratio (PSNR) in image de-nosing. A better denoising effect could be obtained compared with the hard-threshold function, the soft one, the semi-soft one, the cubic polynomial interpolation semi-soft one, and the asymptotic semi-soft one.
Algorithms ; Artifacts ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Photography ; methods ; Wavelet Analysis

Objective To introduce a new method for ultrasonic imaging,3D image fusion technique,and its clinical applications and prospects.Methods A lot of domestic and foreign papers related to 3D image fusion technique were referred to.Results 3D image fusion technique had giant advantage in diagnosis and treatment of tumor.Conclusion 3D image fusion technique is found with a big potential and its extensive application has to take time.

Objective To design a bandstop digital filter for cancelling the 50HZ noise. Methods Procedure and FDATool method were used in processing. Results The filter was tested by a blended sine wave, the experimental result showed that the filter could cancel the 50HZ noise. Conclusion This filter may eliminate a random noise except the 50HZ noise by modifying the procedure simply.

In this paper, LM35 tempeture sensor is tested and shows good linear characteristics between 30 ℃ and 60 ℃, which provides a theoretical basis for making digital thermometer. A/D converting chip and 89c51 single chip micro -processor are employed in this digital body thermometer based on LM35. It possesses such advantages as low cost, high accuracy and excellent stability.