Objective To investigate the distribution of traditional Chinese medicine(TCM)syndrome types in patients with lumbar disc herniation(LDH)and its correlation with serum levels of hypoxia-inducible factor 1α(HIF-1α),NOD-like receptor thermal protein domain associated protein 3(NLRP3)and interleukin 1β(IL-1β)of the patients.Methods Data collection was conducted in 160 patients(LDH group)admitted to the LDH outpatient and inpatient department of Wuhan Hankou Hospital from October 2022 to June 2024 and in 20 healthy volunteers(healthy control group)who underwent physical examination in the hospital during the same period.The levels of serum HIF-1α,NLRP3 and IL-1β were compared between the two groups,and then the correlation of serum HIF-1α,NLRP3 and IL-1β levels with Visual Analogue Scale(VAS)score of pain,Japanese Orthopedic Association(JOA)score and Oswestry Dysfunction Index(ODI)score in LDH patients was explored.The distribution of TCM syndrome types in LDH patients was statistically analyzed,and then the correlation between TCM syndromes and serum HIF-1α,NLRP3 and IL-1β levels in LDH patients was investigated.Furthermore,the correlation between serum HIF-1α,NLRP3,IL-1β levels and prognosis of LDH was analyzed.Results(1)Compared with the healthy control group,the serum levels of HIF-1α,NLRP3 and IL-1β in the LDH group were significantly increased,and the differences were statistically significant(P＜0.01).(2)Spearman correlation analysis showed that VAS and ODI scores were positively correlated with serum HIF-1α,NLRP3 and IL-1β levels,and JOA score was negatively correlated with serum HIF-1α,NLRP3 and IL-1β levels,the differences being statistically significant(P＜0.01).(3)The percentage of TCM syndrome types in 160 patients with LDH in descending order was as follows:blood stasis syndrome(65 cases,40.63%)＞kidney deficiency syndrome(45 cases,28.13%)＞cold-damp syndrome(30 cases,18.75%)＞damp-heat syndrome(20 cases,12.50%).(4)There were statistically significant differences in serum levels of HIF-1α,NLRP3 and IL-1β among LDH patients with different TCM syndromes(P＜0.01):the serum levels in patients with cold-damp syndrome were higher than those in patients with kidney deficiency syndrome(P＜0.05),serum levels in patients with damp-heat syndrome were higher than those in patients with cold-damp syndrome(P＜0.05),and serum levels in patients with blood stasis syndrome were higher than those in patients with damp-heat syndrome(P＜0.05).(5)Multivariate logistic regression analysis showed that serum HIF-1α,NLRP3 and IL-1βwere risk factors for the prognosis of LDH patients,and they were negatively correlated with the prognosis of LDH(P＜0.05 or P＜0.01).Conclusion Blood stasis syndrome is the predominated syndrome type in LDH patients,and then comes kidney deficiency syndrome.The levels of serum HIF-1α,NLRP3 and IL-1β vary in LDH patients with different TCM syndrome types,and are expected to be the potential biological indicators for TCM syndrome differentiation of LDH.Serum HIF-1α,NLRP3 and IL-1β are the risk factors for the prognosis of LDH.

OBJECTIVE To explore the efficacy and safety of pegylated recombinant human granulocyte colony-stimulating factor(PEG-rhG-CSF)in prevention of infections in the children with acute lymphoblastic leukemia(ALL)after chemotherapy.METHODS The clinical data were retrospectively collected from the children with ALL who received vincristine+daunorubicin+pegaspargase+prednisone acetate(VDLP)induction chemo-therapy in pediatrics department of the First Medical Center of Chinese PLA General Hospital from Jan.2018 to Oct.2024,the children were respectively treated with PEG-rhG-CSF or recombinant human granulocyte colony-stimulating factor(rhG-CSF)during the chemotherapy.The incidence of grade Ⅲ/Ⅳ agranulocytosis,the lowest absolute value of neutrophils,duration of grade Ⅳ agranulocytosis,incidence of agranulocytosis accompanied by fever,incidence of infections,use of antibiotics and length of hospital stay were observed and compared between the two groups.RESULTS Totally 47 children with ALL were enrolled in the study and were assigned as the PEG-rhG-CSF group with 24 cases and the rhG-CSF group with 23 cases.There were no significant differences in the incidence rates of grade Ⅲ/Ⅳ agranulocytosis and musculoskeletal soreness between the two groups.The low-est absolute value of neutrophils of the PEG-rhG-CSF group[(0.88±0.87)× 109/L]was higher than that of the rhG-CSF group;the duration of grade Ⅳ agranulocytosis of the PEG-rhG-CSF group[2.50(0.00,6.50)d]was shorter than that of the rhG-CSF group;the incidence of agranulocytosis accompanied by fever(41.67％),inci-dence of infections(41.67％)and the percentage of children treated with antibiotics were lower in the PEG-rhG-CSF group than in the rhG-CSF group;the length of hospital stay of the PEG-rhG-CSF group was shorter than that of the rhG-CSF group;there were significant differences between the two groups(P＜0.05).CONCLUSION The prophylactic use of PEG-rhG-CSF may raise the lowest value of neutrophils,shorten the duration of agranulo-cytosis,decrease the incidence of hospital-associated infections in the ALL children during the induction chemo-therapy,and reduce the use of antibiotics,with the safety favorable.

OBJECTIVE To explore the efficacy and safety of pegylated recombinant human granulocyte colony-stimulating factor(PEG-rhG-CSF)in prevention of infections in the children with acute lymphoblastic leukemia(ALL)after chemotherapy.METHODS The clinical data were retrospectively collected from the children with ALL who received vincristine+daunorubicin+pegaspargase+prednisone acetate(VDLP)induction chemo-therapy in pediatrics department of the First Medical Center of Chinese PLA General Hospital from Jan.2018 to Oct.2024,the children were respectively treated with PEG-rhG-CSF or recombinant human granulocyte colony-stimulating factor(rhG-CSF)during the chemotherapy.The incidence of grade Ⅲ/Ⅳ agranulocytosis,the lowest absolute value of neutrophils,duration of grade Ⅳ agranulocytosis,incidence of agranulocytosis accompanied by fever,incidence of infections,use of antibiotics and length of hospital stay were observed and compared between the two groups.RESULTS Totally 47 children with ALL were enrolled in the study and were assigned as the PEG-rhG-CSF group with 24 cases and the rhG-CSF group with 23 cases.There were no significant differences in the incidence rates of grade Ⅲ/Ⅳ agranulocytosis and musculoskeletal soreness between the two groups.The low-est absolute value of neutrophils of the PEG-rhG-CSF group[(0.88±0.87)× 109/L]was higher than that of the rhG-CSF group;the duration of grade Ⅳ agranulocytosis of the PEG-rhG-CSF group[2.50(0.00,6.50)d]was shorter than that of the rhG-CSF group;the incidence of agranulocytosis accompanied by fever(41.67％),inci-dence of infections(41.67％)and the percentage of children treated with antibiotics were lower in the PEG-rhG-CSF group than in the rhG-CSF group;the length of hospital stay of the PEG-rhG-CSF group was shorter than that of the rhG-CSF group;there were significant differences between the two groups(P＜0.05).CONCLUSION The prophylactic use of PEG-rhG-CSF may raise the lowest value of neutrophils,shorten the duration of agranulo-cytosis,decrease the incidence of hospital-associated infections in the ALL children during the induction chemo-therapy,and reduce the use of antibiotics,with the safety favorable.

Objective:To analyze the genetic mutation characteristics of glucose-6-phosphate dehydrogenase (G6PD) deficiency among infants in Kunming.Methods:A total of 15 533 infants (7 994 males and 7 539 females) born in Kunming from January 1, 2018, to December 31, 2020, with an age range of 2 to 44 days, were selected. G6PD enzyme activity and gene mutation types were detected using fluorescence quantitative analysis, multicolor melting curve analysis (MMCA), and Sanger sequencing. Droplet digital PCR (ddPCR) was used for quantitative analysis of a newly identified variant family to determine the mutant allele proportion in family members. Meanwhile,the protein structure model and pathogenicity prediction of the novel variant were analyzed.Data analysis was conducted using SPSS 26.0. Specifically, chi-square tests were used for the detection rates of G6PD enzyme activity and gene mutations between different genders. One-way analysis of variance (ANOVA) was used for the comparison of enzyme activity among different mutation types.Results:Among 15 533 infants, 143 cases (129 males and 14 females) were tested positive for G6PD activity, with a detection rate of 0.92% (143/15 533). The difference in detection rates between males and females was statistically significant (χ 2=96.76, P<0.001). Out of 89 enzyme activity-positive cases (83 males and 6 females) underwent genetic testing, 77 (72 males and 5 females) were detected by MMCAand other 12 negative samples were underwent further Sanger sequencing, revealing mutations in 6 samples, all of which were males. Among the 83 individuals with gene mutations, 78 had heterozygous mutations, 1 had a homozygous mutation, and 4 had compound heterozygous mutations. A total of 12 mutation types were detected, with G6PD c.487G>A, c.1024C>T, c.1388G>A, and c.1376G>T being the most common, accounting for 74.70% (62/83) of all mutation types. The average G6PD enzyme activity of c.1376G>T was the lowest, and the differences were statistically significant compared to the average enzyme activity of the other three mutations ( P<0.05). One male infant with a newly identified G6PD c.242G>C mutation was detected, predicted to be pathogenic. ddPCR confirmed that the mother of the affected child was a c.242G>C mutant chimera, with a chimera proportion of 6.66%. Conclusions:In the Kunming region, the predominant G6PD deficiency gene mutation is c.487G>A, with the detection of a novel G6PD c.242G>C mutation. The application of ddPCR technology can assist in detecting the proportion of mutation chimeras.

Objective:To investigate the clinical efficacy of 3D printing -assisted percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of lumbar disc herniation (LDH). Methods:A randomized controlled trial was conducted involving 60 patients with LDH who received treatment at The Second Affiliated Hospital of Bengbu Medical University from January 2020 to June 2023. The patients were randomly assigned to two groups using the random number table method, with 30 patients in each group. The control group received conventional PTED, while the observation group underwent 3D printing -assisted PTED. Surgical indicators, including operation time, number of fluoroscopy sessions, and number of needle punctures, as well as clinical efficacy assessed at 3 months postoperatively, were compared between the two groups. Results:The observation group had a significantly lower number of punctures [(2.17 ± 0.68) times] compared with the control group [(5.01 ± 2.15) times]. The operation time in the observation group [(64.31 ± 8.33) minutes] was significantly shorter than that in the control group [(71.89 ± 8.72) minutes]. The observation group had fewer fluoroscopy sessions [(12.27 ± 2.61) times] compared with the control group [(19.20 ± 2.67) times]. All differences were statistically significant ( t = 11.90, 3.63, 10.16, all P < 0.05). At 3 months postoperatively, the rates of excellent and good clinical outcomes according to the MacNab criteria were 96.67% (29/30) in the observation group and 93.33% (28/30) in the control group, with no statistically significant difference between the two groups (χ2 = 0.00, P = 1.000). Conclusion:Preoperative planning utilizing 3D printing technology can effectively assist with the puncture during PTED. It can enhance the efficiency and safety of the procedure, reduce radiation exposure, and exhibit significant application value.

Objective:To investigate the clinical efficacy of 3D printing -assisted percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of lumbar disc herniation (LDH). Methods:A randomized controlled trial was conducted involving 60 patients with LDH who received treatment at The Second Affiliated Hospital of Bengbu Medical University from January 2020 to June 2023. The patients were randomly assigned to two groups using the random number table method, with 30 patients in each group. The control group received conventional PTED, while the observation group underwent 3D printing -assisted PTED. Surgical indicators, including operation time, number of fluoroscopy sessions, and number of needle punctures, as well as clinical efficacy assessed at 3 months postoperatively, were compared between the two groups. Results:The observation group had a significantly lower number of punctures [(2.17 ± 0.68) times] compared with the control group [(5.01 ± 2.15) times]. The operation time in the observation group [(64.31 ± 8.33) minutes] was significantly shorter than that in the control group [(71.89 ± 8.72) minutes]. The observation group had fewer fluoroscopy sessions [(12.27 ± 2.61) times] compared with the control group [(19.20 ± 2.67) times]. All differences were statistically significant ( t = 11.90, 3.63, 10.16, all P < 0.05). At 3 months postoperatively, the rates of excellent and good clinical outcomes according to the MacNab criteria were 96.67% (29/30) in the observation group and 93.33% (28/30) in the control group, with no statistically significant difference between the two groups (χ2 = 0.00, P = 1.000). Conclusion:Preoperative planning utilizing 3D printing technology can effectively assist with the puncture during PTED. It can enhance the efficiency and safety of the procedure, reduce radiation exposure, and exhibit significant application value.

OBJECTIVE: In order to solve the problem that the existing oxygen production technology cannot simultaneously produce pure oxygen, high-purity oxygen, ultra-pure oxygen, and the modular expansion of oxygen production capacity, a new type of electrochemical ceramic membrane oxygen production system was discussed and developed. METHODS: Through the design of the ceramic membrane stack, airflow distributor, heater, double spiral exchanger, thermal insulation sleeve, control panel, control box and auxiliary system in the electrochemical ceramic membrane oxygen generator, a modular oxygen production system is formed. RESULTS: The modular design can produce pure oxygen, high-purity oxygen and ultra-pure oxygen to meet various oxygen consumption needs. CONCLUSIONS The electrochemical ceramic membrane oxygen production system is a new type of oxygen production technology. The main components have no moving parts, no noise, and no pollution. It can produce pure oxygen, high-purity oxygen and ultra-pure oxygen on site, with small size, light weight, and module combination which is suitable for convenient expansion and installation of oxygen consumption.
Oxygen ; Ceramics ; Technology

Hepatectomy and liver transplantation are the most effective radical treatment for patients with hepatocellular carcinoma, but the high recurrence rate after surgery which seriously affects the prognosis of patients cannot be ignored. Microvascular invasion (MVI) is a risk factor for postoperative recurrence and metastasis in patients with hepatocellular carcinoma. There is no consensus or guideline recommendation locally or intermutually on postoperative adjuvant therapy of patients with hepatocellular carcinoma with MVI. Appropriate selection of postoperative adjuvant therapy is worth more in-depth discussion. This article reviews recent and relevant studies on postoperative adjuvant therapy for patients with hepatocellular carcinoma and MVI, including local anti-tumor therapy, systemic chemotherapy, immunotherapy, targeted therapy and combination therapy, with the aim to provide better reference to clinicians in managing these patients with postoperative adjuvant therapy.

Rho-associated kinases (ROCKs) are serine-threonine protein kinases that act downstream of small Rho GTPases to regulate the dynamics of the actin cytoskeleton. Two ROCK isoforms (ROCK1 and ROCK2) are expressed in the mammalian central nervous system. Although ROCK activity has been implicated in synapse formation, whether the distinct ROCK isoforms have different roles in synapse formation and function in vivo is not clear. Here, we used a genetic approach to address this long-standing question. Both Rock1 and Rock2 mice had impaired glutamatergic transmission, reduced spine density, and fewer excitatory synapses in hippocampal CA1 pyramidal neurons. In addition, both Rock1 and Rock2 mice showed deficits in long-term potentiation at hippocampal CA1 synapses and were impaired in spatial learning and memory based on the water maze and contextual fear conditioning tests. However, the spine morphology of CA1 pyramidal neurons was altered only in Rock2 but not Rock1 mice. In this study we compared the roles of ROCK1 and ROCK2 in synapse formation and function in vivo for the first time. Our results provide a better understanding of the functions of distinct ROCK isoforms in synapse formation and function.

Objective: To observe the clinical effects of Zhu Lian's type Ⅰ excitation needling technique for postpartum urinary retention. Methods: A total of 60 cases with postpartum urinary retention were recruited and divided randomly into an observation group and a control group, 30 cases in each group. The control group was treated with routine acupuncture, and stimulated with sparse and dense wave of electric acupuncture for 15 min after arrival of needling sensation, and then the needles were taken out. The observation group was treated with Zhu Lian's type Ⅰ excitation needling technique, by inserting the needles with the quick inserting method, swift and temporary lifting and thrusting technique for shallow insertion for 5 times, by an in-and-out technique, without retaining the needles. Results: The total effective rate was 96.7% in the observation group and 83.3% in the control group. The difference in the total effective rate between the two groups was statistically significant (P<0.05). After the treatment, the first urination time was shorter in the observation group than that in the control group, with a statistical significance (P<0.01). After the treatment, the volume of residual urine after the first urination was less in the observation group than that in the control group (P<0.01). Conclusion: Zhu Lian's type Ⅰ excitation needling technique can effectively promote the voluntary urination and bladder emptying in patients with postpartum urinary retention, and it takes effect faster.