Objective:To construct nomogram model based on lymph node imaging parameters for predicting the risk of lymph node metastasis in lung malignancies.Methods:From November 2020 to September 2022, 34 patients (23 males, 11 females, age (65.8±6.8) years) diagnosed with lung malignancies by pathology at the Affiliated Hospital of Inner Mongolia Medical University were prospectively collected. Based on enhanced CT and 99Tc m-hydrazinonicotinamide-(polyethylene glycol) 4-E((polyethylene glycol) 4-c((Arg-Gly-Asp)fK)) 2 (HYNIC-PEG 4-E(PEG 4-c(RGDfK)) 2; 3PRGD 2)SPECT/CT imaging, referring to the mediastinal lymph node zoning standards formulated by the International Association for the Study of Lung Cancer, lymph nodes with clear pathological properties and imaging locations were included in the study. Lymph nodes were randomly divide into a training group and a validation group at a ratio of 7∶3. Differences of imaging parameters between positive and negative lymph node metastasis were compared by independent-sample t test or Mann-Whitney U test or χ2 test. Parameters with statistical differences were incorporate into the multivariate logistic regression equation, and a joint variable diagnostic model for predicting lymph node metastasis was generated. The potential of the model was evaluate by ROC curve analysis, calibration, and decision curve analysis (DCA). Results:Among 34 patients with malignant lung tumors, 11 had lymph node metastasis. A total of 174 lymph nodes met the inclusion criteria were randomly divided into a training group of 114 nodes and a validation group of 60 nodes. In the training group and validation group, there were statistically significant differences in lymph node length, lymph node short diameter, lymph node length/short diameter, necrosis, lymph node to mediastinal blood pool radioactive count ratio (T/B), lymph node to liver radioactive count ratio (T/L), lymph node to muscle radioactive count ratio (T/M), and lymph node enhancement mode between patients with positive and negative lymph node metastasis ( χ2 values: 3.89-34.06, t values: 2.31-3.87, Z values: from -3.63 to -2.30, all P<0.05). The lymph node edge was different only in the training group ( χ2=5.62, P=0.018). Finally, the lymph node length/short diameter, edge, necrosis, T/B, T/L, T/M, and lymph node enhancement modes were included in the multivariate logistic regression prediction model, with the AUCs of 0.878 and 0.949 in the training and validation groups, respectively. The calibration curve showed good consistency between the predicted results and the actual results, and DCA showed that the nomogram had clinical practicality. Conclusion:The nomogram model constructed based on the imaging parameters of lymph nodes can evaluate the risk of lymph node metastasis in patients with lung malignancies, providing a convenient and objective tool for determining staging and developing treatment plans.

Objective:To construct nomogram model based on lymph node imaging parameters for predicting the risk of lymph node metastasis in lung malignancies.Methods:From November 2020 to September 2022, 34 patients (23 males, 11 females, age (65.8±6.8) years) diagnosed with lung malignancies by pathology at the Affiliated Hospital of Inner Mongolia Medical University were prospectively collected. Based on enhanced CT and 99Tc m-hydrazinonicotinamide-(polyethylene glycol) 4-E((polyethylene glycol) 4-c((Arg-Gly-Asp)fK)) 2 (HYNIC-PEG 4-E(PEG 4-c(RGDfK)) 2; 3PRGD 2)SPECT/CT imaging, referring to the mediastinal lymph node zoning standards formulated by the International Association for the Study of Lung Cancer, lymph nodes with clear pathological properties and imaging locations were included in the study. Lymph nodes were randomly divide into a training group and a validation group at a ratio of 7∶3. Differences of imaging parameters between positive and negative lymph node metastasis were compared by independent-sample t test or Mann-Whitney U test or χ2 test. Parameters with statistical differences were incorporate into the multivariate logistic regression equation, and a joint variable diagnostic model for predicting lymph node metastasis was generated. The potential of the model was evaluate by ROC curve analysis, calibration, and decision curve analysis (DCA). Results:Among 34 patients with malignant lung tumors, 11 had lymph node metastasis. A total of 174 lymph nodes met the inclusion criteria were randomly divided into a training group of 114 nodes and a validation group of 60 nodes. In the training group and validation group, there were statistically significant differences in lymph node length, lymph node short diameter, lymph node length/short diameter, necrosis, lymph node to mediastinal blood pool radioactive count ratio (T/B), lymph node to liver radioactive count ratio (T/L), lymph node to muscle radioactive count ratio (T/M), and lymph node enhancement mode between patients with positive and negative lymph node metastasis ( χ2 values: 3.89-34.06, t values: 2.31-3.87, Z values: from -3.63 to -2.30, all P<0.05). The lymph node edge was different only in the training group ( χ2=5.62, P=0.018). Finally, the lymph node length/short diameter, edge, necrosis, T/B, T/L, T/M, and lymph node enhancement modes were included in the multivariate logistic regression prediction model, with the AUCs of 0.878 and 0.949 in the training and validation groups, respectively. The calibration curve showed good consistency between the predicted results and the actual results, and DCA showed that the nomogram had clinical practicality. Conclusion:The nomogram model constructed based on the imaging parameters of lymph nodes can evaluate the risk of lymph node metastasis in patients with lung malignancies, providing a convenient and objective tool for determining staging and developing treatment plans.

Objective To visually analyze the Chinese and English literature in the research field of Cistanches Herba through bibliometrics;To understand the research status and hotspots of Cistanches Herba;To provide reference for the related research.Methods Literature related to Cistanches Herba in CNKI and Web of Science core databases from January 1,2002 to December 31,2022 was retrieved.VOSviewer 1.6.18 was used for co-occurrence clustering and temporal overlay analysis of authors and keywords,while CiteSpace 6.1.R2 was used for keyword burst analysis.Results Totally 1 631 articles were included,including 1 481 Chinese articles and 150 English articles,and the overall number of publications was on the rise.This field has formed research teams represented by Guo Yuhai from Zhejiang Sci-Tech University,Tu Pengfei from Beijing University of Chinese Medicine,and Chen Jun and Xu Rong from Chinese Academy of Medical Sciences.High frequency keywords included Cistanches Herba,echinoside,phenylethanolside,high performance liquid chromatography,cistanche polysaccharide,artificial cultivation,anti-fatigue,etc.Conclusion The research in this field mainly focuses on TCM therapy,clinical study,etiology and pathogenesis.Network pharmacology,molecular biology,data mining and so on may become the focus of future research of Cistanches Herba.

With the rapid development of network technology and the situation of COVID-19 pandemic, the way people use the Internet has changed dramatically, causing the original network behavior to change again and again, and with its huge impact on people's mental and physical health.This paper deeply elaborate the connotation and development of network behavior and analyzes the impact of network behavior on people's health under COVID-19, then puts forward suggestions to speed up the construction of information infrastructure, strengthen network legislation, improve the information literacy of the whole population, and purify the network environment.

Objective:To evaluate the diagnostic value of coronary angiography-based fractional flow reserve(caFFR)versus a wire-based fractional flow reserve(FFR)in elderly patients with stable ischemic heart disease.Methods:A total of 168 patients(186 vessels)who underwent a pressure wire(PW)-based FFR measurement from Jan.2015 to Dec.2019 in Beijing hospital were enrolled and analyzed retrospectively.Coronary angiography images and matched steady-state aortic pressure of patients were sent to the core laboratory for caFFR measurement under the blind method.All patients were divided into the non-elderly group(<65 years, n=93)and the elderly group(≥65 years, n=75). The diagnostic value of caFFR was evaluated by using the wire-based FFR cut-off value of ≤0.80 as the reference standard.The correlation and consistency of caFFR and wire-based FFR were analyzed, and compared between the non-elderly and elderly groups.Results:The caFFR had a good correlation and consistency with wire-based FFR in the elderly group( r=0.796, P<0.01). In non-aged versus elderly groups, diagnostic accuracy of caFFR was 91.9% versus 93.1%, diagnostic sensitivity of caFFR was 91.8% vs.93.2%, diagnostic specificity of caFFR was 92.3% vs.93.0%, all P>0.05.The area under the receiver-operating characteristic curve of caFFR had no significant difference between the non-elderly and elderly patients(0.964 vs.0.972, Z=0.00823, 95% CI: -0.037-0.052, P>0.05). Conclusions:The caFFR has a good diagnostic correlation and consistency with wire-based FFR in the elderly group, and caFFR's diagnostic performance in the elderly is similar to that in the non-elderly patients.

Objective: To observe the effect of electroacupuncture on the volume of cerebral infarction, apoptosis of cerebral cells and the expression of protein kinase A (PKA) in the cerebral cortex of rats after ischemia and reperfusion so as to explore how electroacupuncture stimulates brain protection. Methods: One hundred and twenty healthy, adult, male Sprague-Dawley rats were randomly divided into a sham operation group, a model group, an electroacupuncture group and an electroacupuncture with pre-stimulation group. All except the rats in the sham operation group received occulusion of the left middle cerebral artery using the intraluminal thread method for 2h and then reperfusion. Before the operation, the rats in the electroacupuncture with pre-stimulation group were given 30 minutes of electroacupuncture at the baihui, dazhui and right neiguan points every day for 5 days. After the operation both the electroacupuncture group and the pre-stimulated group were given that same electroacupuncture regimen. The other two groups received no special treatment. Garcia scoring was used to evaluate the neurological deficits of all of the rats 5 and 10 days after the intervention. Meanwhile, the ischemic volume, apoptosis of cortical cells and PKA-positive cells were determined using flow cytometry and immunohistochemistry after triphenyltetrazolium chloride (TTC) staining. Results: The neurological function of the injured rats was severely impaired, while no neurological deficit was found in the sham operation group. The average Garcia score, cerebral infarction volume, cerebral apoptosis rate and PKA-positive cell expression rate of the electroacupuncture and electroacupuncture with pre-stimulation groups were all significantly better than those of the model group at the same time points. The averages of the electroacupuncture with pre-stimulation group were all significantly superior to those of the electroacupuncture group at the same time points. Conclusions Pre-stimulation using electroacupuncture can promote the recovery of injured nerves after cerebral ischemia and reperfusion, at least in rats. Electroacupuncture′s protective mechanism may be related to its reducing the infarcted volume, inhibiting apoptosis of brain cells and promoting PKA expression.

Objective : To investigate the clinical efficacy of painless oral anesthesia combined with piezosurgery compared with ordinary cassette syringe combined with high speed turbine handpiece in the Fenestration operation of orthodontic traction of impacted maxillary anterior teeth. Methods : A total of 128 cases of impacted maxillary anterior teeth were treated with Fenestration operation and orthodontic traction, and the patients were randomly divided into two groups. Group A (64 patients) received painless oral anesthesia combined with piezosurgery. Group B (64 patients) received ordinary cassette syringe combined with high speed turbine handpiece. The cooperation of fenestration and the swelling and pain after operation were compared between the 2 groups. Results : The proportion of 0 and Ⅰ in the anesthesia group was 45.3% and 31.3% respectively, and the conventional group 0 and I accounted for 32.8% and 20.3% respectively. The difference between the two groups was statistically significant (Z=-2.676, P < 0.05). The pateints' cooperative degree in group A was better than that in group B (P < 0.05). The ratio of pain and swelling in the anesthesia instrument combined with Piezosurgery group was 81.2%, the conventional group had a grade I ratio of 59.4%, and the anesthesia instrument combined with Piezosurgery group was lighter than the conventional group, The difference was statistically significant (Z=-2.777, P < 0.05); anesthesia combined with Piezosurgery group after surgery accounted for 81.2% of the swelling, the conventional group of pain accounted for 71.9%, There was significant difference between the two groups (Z=-2.097, P < 0.05). Symptoms and signs as swelling and pain degree in group A were relatively minor than those in group B (P < 0.05). Conclusion It is worthy clinical promotion to use Painless oral anesthesia combined with piezosurgery in the fenestration and orthodontic traction of impacted maxillary anterior teeth, for its better cooperation and minor post-surgery reaction.

Objective: To explore the clinical application of cone beam CT in the diagnosis of traumatic anterior teeth before orthodontic treatment in order to reduce the risk of orthodontic treatment failure due to adhesion. Methods : 48 fixed orthodontic treatment cases with the history of anterior teeth trama were selected. The anterior teeth were examined by CBCT to exclude root and alveolar bone adhesions. Results : 3 cases of root-alveolar bone adhesion were found, and the upper anterioa teeth of the remaining 45 cases were successfully moved by orthodontic treatment. Conclusion CBCT examination is recommended to check the periodontal ligament in patients of fixed orthodontic treatment with the history of anterior teeth trauma in order to exclude the root-alveolar bone adhesion cases.

Objective To observe the influence of swimming training on the expression of nerve growth factor (NGF) and neurotrophins-3 (NT-3) in rats with cerebral infarction,and to explore the underlying neuroprotection mechanism of exercise training on cerebral infarction.Methods Forty-five healthy male Sprague-Dawley rats were randomly divided into a sham-operation group,a control group and a training group,with 15 rats in each group.Each group was further divided into a 3-day,7-day and 14-day subgroups,which amounts to 9 groups.To establish animal model of cerebral ischemia-reperfusion in rats,the intraluminal thread method was applied to cause left middle cerebral artery occlusion (MCAO) for 2 h before reperfusion.The rats of the training group were given swimming training for 10 min,once daily,while those of the sham-operation and control groups were not given any training.Neurological deficits were assessed using Bederson scores.The expression of NGF mRNA and NT-3 mRNA in the ischemiareperfusion pallium was examined using reverse transcription-polymerase chain reaction (RT-PCR).Results The rats of the sham-operation group showed no neurological deficits.At the same time points,the average Bederson scores of the training group were significantly lower than the control group,but significantly higher than the sham group.Moreover,the 14 d training group had the lowest Bederson score (1.20 ±0.45),compared to the value 3 and 7 days after modeling.The expression of NGF mRNA and NT-3 mRNA of ischemic cerebral cortex in the training group was significantly improved when compared to the sham-operation group or the control group.On day 14,the expression of the NGF mRNA (0.66 ± 0.07),and the NT-3 mRNA (0.79 ± 0.06),were significantly higher than those on day 3 and 7.Conclusions Swimming training could increase the expressions of NGF mRNA and NT-3 mRNA in the ischemic cerebral cortex.It might be one of the key mechanisms that exercise training could promote the recovery of damaged neurological function in rats with cerebral infarction.

Objective To examine the expression of CD9 protein in pancreatic cancer tissues and adjacent tissues,and to analyze its relation with the progress and prognosis of pancreatic cancer.Methods From September 2005 to December 2009,surgical resected cancer tissues and adjacent tissues of 90 patients with pancreatic cancer and their clinical data were collected.The expression of CD9 protein in pancreatic cancer tissues and adjacent tissues was detected by immunohistochemistry,and its relationship with clinicopathological features was analyzed.Chi-square test was performed for comparison of ratios between groups.Overall survival (OS) analysis of 90 patients after surgery was performed.Results The high expression rate of CD9 protein (64.4％,58/90) in cancer tissue was significantly higher than that in cancer adjacent tissue (45.6％,41/90),the difference has statistically significant (χ2 =6.847,P＜0.05).CD9 protein was highly expressed in most of pancreatic cancer tissue which was well differentiated or without lymph node metastasis (74.6％ (50/67) vs 39.1％ (9/23),χz =9.554,P＜0.01; 50.0％(17/34) vs 73.2％(41/56),χ2 =5.856,P＜0.05 respectively).However,the expression of CD9 was not correlated with gender and age (both P＞0.05).OS and progression-free survival (PFS) of the patients with CD9 highly expressed were significantly longer than those with low expression of CD9 (median OS:33.0 months vs 7.0 months,χ2 =15.400 P＜0.01.Median PFS:30.5 months vs 5.0 months,χ2 =13.750,P＜0.01).Conclusion CD9 protein is a kind of protein related with the invasive ability of pancreatic cancer,which may play a role in progression and metastasis of pancreatic cancer and can help to determine the prognosis to a certain extent.