Objective To explore the correlations of serum interleukin-4(IL-4),leptin and chemerin levels with severity of asthma in obese children and their predictive values for asthma control.Methods A total of 102 obese children with asthma were selected as study group,and another 102 healthy obese children were selected as control group.The serum levels of IL-4,leptin and chemerin were compared between the two groups.The study group was divided into mild(n=34),moderate(n=42)and severe(n=26)asthma groups based on severity of disease,and the serum levels of IL-4,leptin and chemerin were compared among different groups.The correlations of serum IL-4,leptin and chemerin with disease severity were analyzed.After 4 weeks of treatment,the 102 children with asth-ma were divided into controlled group(n=82)and uncontrolled group(n=20)based on asthma con-trol status.Clinical data and serum levels of IL-4,leptin and chemerin were compared between the two groups,and their impacts on asthma control were analyzed.The predictive values of serum IL-4,leptin and chemerin levels for asthma control were evaluated.Results The serum levels of IL-4,leptin and chemerin showed significant gradual increase trends among controlled children,mild asthma children,moderate asthma children,and severe asthma children(P＜0.001).Spearman correlation analysis showed positive correlations of serum IL-4,leptin and chemerin with disease severity(P＜0.001).The serum levels of IL-4,leptin and chemerin in the uncontrolled group were significantly higher than those in the controlled group(P＜0.001).Logistic regression analysis showed that serum IL-4(OR=1.514,95％CI,1.117 to 2.051),leptin(OR=1.534,95％CI,1.234 to 1.907),and chemerin(OR=1.418,95％CI,1.026 to 1.959)after adjusting for disease duration were still the independent risk factors for asthma control(P＜0.05).The areas under the curve(AUC)for pre-dicting asthma control by single and combined detections of serum IL-4,leptin and chemerin were 0.807(95％CI,0.717 to0.879),0.801(95％CI,0.711 to0.874),0.834(95％CI,0.748 to 0.901)and 0.932(95％CI,0.864 to 0.972),respectively.The AUC for combined prediction was significantly better than that for individual predictor(P＜0.05).Conclusion The serum lev-els of IL-4,leptin and chemerin in obese children with asthma are positively correlated with disease severity,and they are independent risk factors for asthma control.Combined detection of these three indicators has a higher predictive value for asthma control.

Objective To analyze the expression characteristics of excision repair cross-complementing 1 (ERCC1), topoisomeraseⅡ (TOPOⅡ), ribonucleotide reductase M1 (RRM1), β3-tubulin and thymidylate synthase (TS) in lung cancer and their associations with the pathological types. Methods The immunohistochemical EnVision method was used to determine the expression of ERCC1, TOPOⅡ, RRM1,β3-tubulin and TS in 548 patients who were diagnosed as lung cancer from January 2011 to December 2014. Variance analysis was performed to analyze their expression characteristics among different pathological types and correlation. Results The expression positive rates of ERCC1, TOPOⅡ, RRM1, β3-tubulin and TS were 61.86 % (339/548), 91.06 % (499/548), 62.59 % (343/548), 73.18 % (401/548) and 70.44 % (386/548), respectively. The expression of ERCC1 was weak positive mostly (P<0.05), meanwhile the expression of TOPOⅡ was medium-strong positive mostly (P<0.05). In ERCC1 group, the positive rate of squamous cell carcinoma was higher than that of adenocarcinoma [57.39 % (167/291) vs. 42.61 % (124/291), P=0.000]. In weak positive of TOPOⅡ group, the proportion of adenocarcinoma was higher than that of squamous cell carcinoma [23.58 % (100/137) vs. 8.73 % (37/137), P=0.000]. In medium-strong positive of TOPOⅡ group, the proportion of squamous cell carcinoma was higher than that of adenocarcinoma [47.41 % (201/287/) vs. 20.28%(86/287), P=0.000]. The expressions of ERCC1, TOPOⅡ, RRM1,β3-tubulin and TS were irrelevant (r=0.4, P=0.397). Conclusions The expressions of ERCC1 and TOPOⅡ are higher in squamous cell carcinoma than those in adenocarcinoma. The expression of ERCC1 is weak positive mostly, meanwhile the expression of TOPOⅡis medium-strong positive mostly. There is no correlation between them.

OBJECTIVETo increase local blood supply of bone graft, a novel posterior lumbar spine fusion method with orthotopic paraspinal muscle-pediculated bone flaps was constructed, and the fusion rate and clinical effect.were observed.

METHODSFrom June 2007 to December 2010, 117 patients of lumbar spinal stenosis or lumbar destabilization treated with the novel posterior lumbar fusion method were studied, 49 males and 68 females, aged from 40 to 77 years, average 61.5 years. Clinical effect was evaluated by JOA and VAS score preoperatively and postoperatively, and the fusion result was evaluated by three-dimensional CT reconstruction postoperatively.

RESULTSSeventeen cases lost of follow up, the rest were followed up from 7 to 38 months, average 19 months. There was significant difference between pre- and postoperative JOA and VAS score (P < 0.01), the preoperative JOA score was 10.3 ± 1.9, and 25.4 ± 4.2 at the latest follow-up, the improvement rate was 81.0% ; the preoperative VAS score was 8.5 ± 0.8, and 2.3 ± 0.4 at the latest follow-up. The three-dimensional CT reconstruction showed that 126 of the 133 segments formed solid fusion in 100 patients who completed the follow-up, the fusion rate was 94.7%.

CONCLUSIONThe novel posterior lumbar fusion method make the bone graft position more precise, stable and increases the fusion rate, which can effectively reduce pseudarthrosis and have a promising clinical effect.

Adult ; Aged ; Bone Transplantation ; Female ; Follow-Up Studies ; Humans ; Lumbar Vertebrae ; Male ; Middle Aged ; Spinal Fusion ; methods ; Spinal Stenosis ; surgery ; Treatment Outcome