The welfare and ethics of laboratory animals are the ethical principles and behavioral norms that need to be followed in conducting animal-based scientific research, breeding and managing laboratory animals, and supervising and regulating such activities. The level of protection of laboratory animal welfare and ethics is closely related to the development of science and technology, which has become a widely recognized international consensus. At present, Guangdong Province is accelerating the construction of a high-level science and technology innovation province and the Guangdong-Hong Kong-Macao Greater Bay Area International Science and Technology Innovation Center. Guangdong Province should rely on its advanced governance capacity in the field of laboratory animal science and technology ethics to promote the high-quality development of its laboratory animal science and technology sector. Based on the management laws, regulations, and institutional mechanisms of laboratory animals in China, this paper explores the optimization of the laboratory animal science and technology ethics governance system, which includes the institutional guarantees, responsibility systems, ethical review and supervision mechanisms, and education and outreach. Through methods such as literature research, questionnaire surveys, and interview investigations, an empirical study of the laboratory animal science and technology ethics governance system in Guangdong Province has been conducted. Analysis of literature and research results shows that Guangdong Province has basically established a laboratory animal management system, collaboration mechanism, supervision mechanism, and education and training system that meet the current requirements of the laboratory animal science and technology ethics governance system in China. However, there are still problems such as an incomplete laboratory animal science and technology ethics supervision mechanism, an underdeveloped operation mechanism of review institutions, insufficient attention paid by laboratory animal units to the ethical review of animal experiments, inconsistent ethical review standards, and a lack of professional ethical education and training for ethics review personnel. Therefore, optimization measures such as improving the laboratory animal science and technology ethics review system, strengthening supervision and inspection, further strengthening the accountability of responsible entities, formulating review norms, and enhancing hierarchical and classified education and training are proposed, to provide a theoretical basis for promoting the normalized and long-term governance of laboratory animal science and technology ethics in Guangdong Province.

The clinical diagnosis and treatment of kidney transplantation involve a large amount of high-dimensional, nonlinear and multimodal complex data. Traditional statistical methods make it difficult to fully identify the underlying patterns. However, artificial intelligence (AI) technology has stronger capabilities in data integration and analysis, and is particularly adept at handling complex data. This article reviews the application of AI in the field of kidney transplantation, covering the role of deep learning models in the quantitative assessment of donor kidney quality in the pre-transplantation stage, the value of virtual biopsy systems and the optimization strategies for donor-recipient matching, as well as the dynamic prediction of graft survival rate by the iBox system in the post-transplantation stage, the advantages of AI models in non-invasive monitoring of rejection and the application results of individualized dose prediction of immunosuppressive drugs. Although the clinical application of medical AI is still limited by insufficient model interpretability, questionable generalization ability, and ethical and legal risks, technologies such as federated learning and digital twins are expected to solve the problems of data privacy and simulation prediction, and further promote the precise development of kidney transplantation.

Objective:To evaluate the clinical efficacy of returning, squeezing, patting and pulling orthopaedic manipulation combined with pelvic fabric tape fixation for the treatment of postpartum pubic symphysis separation.Methods:The clinical data of 80 patients with postpartum pubic symphysis separation from June 2015 to March 2019 were retrospectively analyzed, and all of them were given orthopaedic manipulative therapy using return squeezing and patting and pulling, once a week, for a total of 3 times. After the manipulative treatment, the patients were instructed to brake the pelvic fixation straps for not less than 8 h per day, and digital X-ray (DR) pelvic radiographs or ultrasound tests were performed before and after the treatment to measure the distance between the pubic symphysis. VAS scale was used to assess the degree of pain, and the Oswestry dysfunction index (ODI) was used to assess the degree of dysfunction. The clinical efficacy was evaluated.Results:After treatment of 80 patients, 6 showed significant improvement, 69 showed improvement, and 5 showed no improvement, with a total effective rate of 93.8%. Compared with before group, the inter-pubic symphysis distance [(15.09±3.10) mm, (12.01±4.36) mm, (9.64±0.30) mm, (8.18±1.56) mm vs. (19.35±1.08) mm, F=254.64] were significantly smaller at 1 week, 2 weeks, 3 weeks, and 1 month ( P<0.001); VAS scores (2.90±1.24, 1.29±0.88, 0.84±0.43, 0.56±0.32 vs. 6.11±2.93, F=122.60) were significantly lower than before treatment ( P<0.001); ODI (28.09±4.30, 22.01±4.95, 20.64±0.41, 14.18±1.36 vs. 45.43±4.01, F=734.17) were significantly reduced ( P<0.001). Conclusion:Returning, squeezing, patting and pulling orthopaedic manipulation combined with pelvic fabric tape fixation can quickly restore the separation distance of the pubic symphysis, reduce local pain and improve lumbosacral function.

As a model organism that bridges the gap between cells and traditional mammals,the zebrafish has broad application prospects in radiation medicine research.Among its unique advantages are its characteristics of a high homology with human genes,high fertility,short embryonic period,and transparent and easy to observe embryos,making it an important tool in radiation medicine research.Recently,remarkable progress has been made in the application of zebrafish to investigate low-dose radiation biological effects,radiation therapy,and radiation damage prevention and treatment,key areas of radiation medicine.In this paper,these applications are reviewed;we explore the value of zebrafish in radiation medicine research and provide a reference for experimental research in related fields.

This consensus will introduce the characteristics of fillers used in the surgical cavities of domestic nasal surgery patients based on relevant literature and expert opinions. It will also provide recommendations for the selection of cavity fillers for different nasal diseases, with chronic sinusitis as a representative example.
Humans ; Nasal Cavity/surgery* ; Nasal Surgical Procedures ; China ; Consensus ; Sinusitis/surgery* ; Dermal Fillers

Aim To investigate the effect of adenovirus(ADV)-mediated Profurin(PF)expression on the plaque stability of ApoE-/-mice.Methods ApoE-/-mice were fed with high-fat diet for 8 weeks,and then treated with ADV-mediated PF intervention,followed by high-fat diet for 4 weeks.Aortic roots were isolated for atherosclerotic plaque area analysis and immunohistochemical analysis.Plasma phospholipid transfer protein(PLTP)activity was detec-ted by fluorescence donor essay,plasma total cholesterol(TC)and triglyceride(TG)were measured by enzyme assay kits,and fast protein liquid chromatography was used for lipoprotein profile analysis.Results Compared with the con-trol group,the plasma TC and TG levels,PLTP activity and circulating tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in ADV-PF group were significantly decreased(P＜0.01).In the ADV-PF group,there was no significant change in atherosclerotic lesions on the inner surface of the full-length aorta,but the plaque area and lipid area in the aortic root were reduced(P＜0.01),the content of macrophages was significantly decreased(P＜0.01),and the smooth muscle cells and collagen area were not significantly different.The content of matrix metalloproteinase-9 in plaque was signifi-cantly decreased(P＜0.05).Conclusion Overexpression of PF can alleviate atherosclerosis and reduce the levels of circulating inflammatory factors to a certain extent,and effectively improve the plaque stability of ApoE-/-mice.

Objective To explore the significance of detecting serum semaphorin 4D(Sema4D),soluble growth-stimulation expression gene 2 protein(sST2),and Omentin-1 in patients with hypertrophic cardiomyopathy(HCM)accompanied by atrial fibrillation(AF),and to provide reference for clinical diagnosis and treatment.Methods A total of 90 patients with HCM and atrial fibrillation admitted to Tsinghua University Yuquan Hospital(Tsinghua University Integrated Traditional Chinese and Western Medicine Hospital)from January 2022 to December 2023 were selected as the HCM-AF group,and another 90 patients with sinus rhythm HCM in the same stage were selected as the sinus rhythm HCM group.The serum levels of Sema4D,sST2,Omentin-1 and cardiac function indicators[left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD)]were compared between the two groups.The correlation between serum Sema4D,sST2,Omentin-1 and cardiac function indicators,as well as the severity of atrial fibrillation in HCM was analyzed.The predictive value of three indicators for atrial fibrillation in HCM was analyzedand the predictive efficacy of percutaneous radiofrequency ablation(PRFA)for the recurrence of atrial fibrillation in HCM patients.Results The serum levels of Sema4D and sST2 in the HCM-AF group were higher than those in the sinus rhythm HCM group,while the levels of Omentin-1 and LVEF were lower than those in the sinus rhythm HCM group,with statistically significant differences(t=2.517～12.325,all P<0.05).The serum levels of Sema4D and sST2 in the persistent subgroup were higher than those in the paroxysmal subgroup,while the levels of Omentin-1 and LVEF were lower than those in the paroxysmal subgroup,the differences were statistically significant(t=2.635～15.931,all P<0.05).Spearman/Pearson correlation analysis showed that serum Sema4D and sST2 were negatively correlated with LVEF(r=-0.57,-0.609,all P<0.001),while Omentin-1 was positively correlated with LVEF(r=0.593,P<0.001).Serum Sema4D and sST2 were positively correlated with the severity of AF in HCM(r=0.625,0.631,all P<0.001),while Omentin-1 was negatively correlated with the severity of AF in HCM(r=-0.617,P<0.001).The serum levels of Sema4D and sST2 in the recurrence subgroup were higher than those in the non-recurrence subgroup,while the serum level of Omentin-1 was lower(t=12.805,7.049,13.345,all P<0.05).The receiver operator characteristic(ROC)curve results showed that the AUC(95%CI)of serum Sema4D,sST2 and Omentin-1 combined to predict atrial fibrillation in HCM was 0.916(0.865～0.952),higher than the individual predictions of the three(Z=3.621,3.657,0.318,all P<0.05).The AUC(95%CI)of serum Sema4D,sST2 and Omentin-1 combined to predict the recurrence of atrial fibrillation in patients with HCM after radiofrequency ablation was 0.931(0.857～0.973),higher than the individual predictions of the three(Z=4.115,3.657,3.853,all P<0.05).Conclusion The levels of serum Sema4D and sST2 in the serum of patients with HCM complicated with atrial fibrillation were elevated,while the level of Omentin-1 was drcreased.Detecting serum Sema4D,sST2 and Omentin-1 has high performance in predicting the occurrence of atrial fibrillation in patients with HCM and the recurrence of atrial fibrillation after radiofrequency ablation therapy.

X-ray is usually used to determine anatomy and localization during conventional permanent pacemaker implantation.But X-ray exposure might induce radiation injury to fetus.In this paper,we reported 2 cases successful double cavity permanent pacemaker implantation in pregnant women woman at 13 weeks of gestation under the guidance of transthoracic echocardiography.The postoperative pacing parameters were good,the whole process was finished with zero radiation,and there were complications during pregnancy.Both cases resulted in full-term pregnancies and the natural delivery of healthy newborns.Pacemaker electrodes were in normal position as confirmed by post-delivery X-ray examinations.

Postoperative infection of internal fixation of closed fractures the lower limbs in adults represents a devastating complication, characterized by diagnostic challenges, prolonged treatment duration and high disability rates. Current management of these infections faces multiple challenges, such as difficulties in early accurate diagnosis, and various controversies about the treatment plan, leading to poor overall diagnosis and treatment results. To address these issues, based on evidence-based medicine and principles with emphasis on scientific rigor, clinical applicability and innovation, the Trauma Branch of the Chinese Medical Association, Orthopedic Branch of the Chinese Medical Doctor Association, Orthopedics Branch of the Chinese Medical Association, and Trauma Orthopedics and Polytrauma Group of the Resuscitation and Emergency Committee of the Chinese Medical Doctor Association have collaboratively organized a panel of relevant experts to develop the Guideline for diagnosis and treatment of infection after internal fixation of closed lower limb fractures in adults ( version 2025). The guideline proposed 10 recommendations, aiming to provide a foundation for standardized diagnosis and treatment of postoperative infection in adults with closed lower limb fractures.