Objective To retrospectively analyze clinical features,treatment methods and efficacy of giant cell tumor of bone in proximal tibia,and to investigate risk factors affecting tumor recurrence and functional outcomes.Methods A total of 250 patients with giant cell tumor of bone in proximal tibia confirmed by pathology,who had undergone surgical treatment from March 2000 to July 2014,were enrolled in this study.There were 132 males and 118 females,with an average age of (34.59±12.86) years.A total of 140 patients who were followed up for more than 3 years were included in this study,and there were 72 males and 68 females,with an average age of (34.46± 11.96) years.There were 11 cases of Campanacci grade Ⅰ,58 cases of grade Ⅱ,71 cases of grade Ⅲ and pathological fracture of 47 cases.According to surgical methods,they were divided into bone grafting group (49 cases),bone cement filling group (34 cases),prosthesis group (46 cases) and others group (11 cases).The epidemiology,clinical and radiographic features and risk factors affecting tumor recurrence and functional outcomes were analyzed.Results A total of 140 patients were followed up,the follow-up period was 36-324 months,with an average of 95.4 months,and the median follow-up time was 88 months.Recurrence was found in 26 cases,and recurrence rate was 18.57％,with an average recurrence interval of 25.85 months.Recurrence was found in 17 cases in the first 2 years.The 5-year free survival rate was 77.60％.The recurrence rates were 18.37％ in bone grafting group,20.59％ in bone cement filling group,15.22％ in prosthesis group and 27.27％ in the others group,no statistically difference was found on recurrence rate and free survival rate (P=0.805,P=0.558).Recurrence was not related to all kinds of factors.A variety of related factors affecting postoperative recurrence were analyzed,sex,the first diagnosis of the original recurrence,left and right side,whether the eccentricity,fracture,cortical bone destruction,soft tissue mass,surgical methods,high-speed grinding,auxiliary application,and there was no significant correlation between recurrence and these factors.The MSTS 93 score was 25.26±4.31.Function of the primary patients was better than that of recurrence (P=0.044).Function of the patients treated with curettage with or without internal fixation was better than that with segmental resection (P=0.011).Function of the patients treated with grafting or bone cement filling was better than that with prosthesis or allograft-prosthesis reconstruction (P=0.004).There were no significant correlation between MSTS function score and gender,left and right side,whether the eccentricity,whether fractures,cortical bone destruction (Campanacci grade),whether there is soft tissue mass,whether the use of assisted inactivation,whether the use of grinding or internal fixation.Conclusion Various surgical methods had no significant effect on the recurrence of proximal tibial GCT,as for GCT in proximal tibia,there is no relation between recucrrence and related factors.Whether primary tumor and surgical methods are two important factors affecting limb function.

Objective To provide scientific evidence for studying biological characteristic of cockroaches and control countermeasures by investigating the species distribution and inroads situation of cockroaches in civil aircraft.Methods In August 2015,the onsite investigations were done for 19 A320 and A321 aircrafts harbored at Sanya airport and Haikou airport for getting the cockroach density.The difference of cockroache density was compared by aircraft type,aircraft age and the 4 areas of the aircraft.Results The total invasion rate was 84.21％ on 19 aircrafts.Blauella germanica is the dominant specie.The invasion rates of cockroaches was not statistically significant neither by the comparison of aircraft type (x2 =1.362,P＞0.05) nor by the comparison of aircraft age (x2=0.281,P ＞ 0.05).But statistical significance on cockroaches density was found in the comparison of 4 areas of aircrafts (x2=14.629,P＜0.01).Conclusions The comprehensive countermeasures,including environmental,physical and chemical controls,should be adopted according to the aircraft structure,operation and safety requirements.

Objective To provide scientific evidence for studying biological characteristic of cockroaches and control countermeasures by investigating the species distribution and inroads situation of cockroaches in civil aircraft.Methods In August 2015,the onsite investigations were done for 19 A320 and A321 aircrafts harbored at Sanya airport and Haikou airport for getting the cockroach density.The difference of cockroache density was compared by aircraft type,aircraft age and the 4 areas of the aircraft.Results The total invasion rate was 84.21％ on 19 aircrafts.Blauella germanica is the dominant specie.The invasion rates of cockroaches was not statistically significant neither by the comparison of aircraft type (x2 =1.362,P＞0.05) nor by the comparison of aircraft age (x2=0.281,P ＞ 0.05).But statistical significance on cockroaches density was found in the comparison of 4 areas of aircrafts (x2=14.629,P＜0.01).Conclusions The comprehensive countermeasures,including environmental,physical and chemical controls,should be adopted according to the aircraft structure,operation and safety requirements.

Objective To explore the efficacy and adverse reactions of diminishing scheme of cefaclor sustained re-lease tablets in the treatment of recurrent urinary tract infection(RUTI).Methods 60 RUTI patients in a hospital were divided into treatment group(n=30)and control group (n=30),patients in treatment group were treated with diminishing scheme of cefaclor sustained release tablets,patients in control group were treated with diminishing scheme of levofloxacin tablets,clinical therapeutic efficacy and adverse reactions of two groups were observed. Results The curative rate in treatment group was higher than control group ([80.00%,n =24]vs [53.33%,n =16])(χ2 =4.80,P =0.028).The incidence of RUTI in treatment group was lower than control group ([6.67%,n=2]vs [26.67%,n=8])(χ2 =4.32,P =0.038).Incidence of adverse reactions in treatment group was lower than control group (16.67% vs 50.00%)(χ2 =7.50,P =0.006).Conclusion The diminishing scheme of cefaclor sus-tained release tablets in the treatment of RUTI has good curative efficacy,low recurrence rate,fewer adverse reac-tions,and can be used for the treatment of recurrence of RUTI.

In the process of designing genechip probe for detecting pathogenic microorganism, the selection of single nucleotide polymorphism (SNP) combination is of great importance. At present, there is no automatic design method. This work is hard and the result is not always well. A new approach for selecting SNP combination is presented in this paper. Genetic algorithm is used to search optimal solution on the basis of classification ability of SNP combination, which is evaluated by the rough set theory. Other related experimental parameters are also incorporated. Experimental results show that the method can find out the best SNP combination pattern efficiently and accurately, thus demonstrating the reliability of this new method for designing the genechip probe.
Algorithms ; Base Sequence ; Brucella ; isolation & purification ; Humans ; Molecular Sequence Data ; Oligonucleotide Array Sequence Analysis ; methods ; Polymorphism, Single Nucleotide ; Salmonella enterica ; isolation & purification