Objective To explore the effect of melatonin(MT)-modified PEEK implant assisted by polydopamine(PDA)coating on osteointegration in osteoporotic rats.Methods MT was adhered to PEEK implants with PDA coating as carrier.The physicochemical properties of the materials were analyzed by SEM image,water contact angle,FTIR and protein adsorption experiment.OVX-rBMSCs were inoculated on the surface of PEEK sheet and cultured.The cytoskeleton was stained and cell adhesion morphology was observed.Cell proliferation activity was evaluated by CCK-8 assay;key enzyme activities for osteogenic differentiation were analyzed by ALP stai-ning,and expression levels of osteoblast-related genes COL-1,Runx2,OPN,OCN,BMP-2 and ALP were detected by quantitative real-time PCR.In addition,implants were implanted into the femur of osteoporotic rats and bone volume on the implant surface was de-tected and quantified by Micro-CT.Results MT was successfully loaded on PEEK;the cell adhesion was better,and the proliferation activity and osteogenic differentiation ability were significantly higher than those of control group(P＜0.01).In the rat osteoporosis mod-el,there was more new bone formation around the modified PEEK implant(P＜0.01).Conclusion MT-modified PEEK implants have excellent biocompatibility and improve osteointegration in an osteoporotic environment.

Objective:To summarize the patient profiles and therapeutic efficacies of ABO-incompatible living-related kidney transplantations at 19 domestic transplant centers and provide rationales for clinical application of ABOi-KT.Methods:Clinical cases of ABO-incompatible/compatible kidney transplantation (ABOi-KT/ABOc-KT) from December 2006 to December 2009 were collected. Then, statistical analyses were conducted from the aspects of tissue matching, perioperative managements, complications and survival rates of renal allograft or recipients.Results:Clinical data of 342 ABOi-KT and 779 ABOc-KT indicated that (1) no inter-group differences existed in age, body mass index (BMI), donor-recipient relationship or waiting time of pre-operative dialysis; (2) ABO blood type: blood type O recipients had the longest waiting list and transplantations from blood type A to blood type O accounted for the largest proportion; (3) HLA matching: no statistical significance existed in mismatch rate or positive rate of PRA I/II between two types of surgery; (4) CD20 should be properly used on the basis of different phrases; (5) hemorrhage was a common complication during an early postoperative period and microthrombosis appeared later; (6) no difference existed in postoperative incidence of complications or survival rate of renal allograft and recipients at 1/3/5/10 years between ABOi-KT and ABOc-KT. The acute rejection rate and serum creatinine levels of ABOi-KT recipients were comparable to those of ABOc-KT recipients within 1 year.Conclusions:ABOi-KT is both safe and effective so that it may be applied at all transplant centers as needed.

Objective To evaluate the curative effect of non-invasive positive pressure ventilation combined with naloxone on acute exacerbation of chronic obstructive pulmonary disease ( COPD) complicated with type II respiratory failure .Methods The pa-tients in the treatment group were additionally given naloxone intravenously on the basis of conventional treatment combined with the non-invasive positive pressure ventilation , while the patients in the control group were administered with non-invasive positive pressure ventilation in addition to conventional treatment .Then, changes in blood gas indicators , ventilation time and duration of hospitalization before and after treatment were observed , and analyses were made between the 2 groups.Results After treatment, the blood gas indi-cators of the treatment group were superior to those of the control group (P<0.05).The average ventilation time of the treatment group was(5.6 ±2.0)days, while that of the control group was(7.1 ±3.0)days;the length of hospitalization for treatment group was (10.8 ±2.5) days and that for the control group was (13.2 ±3.0)days respectively.Statistical significance could be seen , when comparisons were made between the 2 groups(P<0.05).Conclusion The curative effect of non-invasive positive pressure ventilation plus naloxone was confirmative in the treatment of acute exacerbation of chronic obstructive pulmonary disease complicated with type II respiratory fail -ure, and it could significantly shorten ventilation time and hospital stay .

Objective To explore the association between the levels of heat shock protein 27 (HSP27) and 70 (HSP70) and pulmonary function and life quality in the patients with chronic obstructive pulmonary disease ( COPD) .Methods Forty-five patients with acute exacerbation of COPD admitted for treatment in our hospital from February 2015 to April 2016 were enrolled for the study , and were divided into the observation group (n=45) and the control group (50 healthy subjects) .Serum samples were collected from the patients of the 2 groups.The levels of serum HSP27 and HSP70 were detected by ELISA .The life quality of the COPD patients was evaluated by using the CAT scale , and the association between the levels of HSP 27 and HSP70 and pulmonary function and life quality was discussed in the paper .Results As compared with those of the control group , the expression levels of HSP 27 and HSP70 in the pa-tients of the observation group were significantly increased [(3 590.25 ±503.42) ng/L vs (1 974.08 ±527.38) ng/L] versus [(331.82 ±18.61)ng/L vs(162.73 ±51.52)ng/L] (P<0.01).Statistical significance was shown when comparisons were made be-
tween them(P<0.01).Spearman sequence analysis indicated that serum levels of HSP 27 and HSP70 were negatively correlated with FEV l%(r=-0.674, -0.569, P<0.05).CAT scores of the observation group were negatively correlated with FEV l% ( r=-0.43, P<0.05), and the serum levels of HSP27 and HSP70 in the observation group were positively correlated with CAT scores (r=0.622,0.53, P<0.05).Conclusion The CAT scale could effectively evaluate the life quality of COPD patients .HSP27 and HSP70 were involved in the process of inflammatory reaction and oxidative stress of COPD , which was of diagnostic value and clinical significance in the treatment of patients with acute exacerbation of COPD .

Objective: To investigate the expression changes of Bcl-2-associated athanogene 1(BAG-1) and its regulatory effect on the glucocorticoid receptor(GR) activity in rat alveolar macrophages in conditions of cell inflammation and glucocorticoid therapy.Methods: The expression changes of BAG-1 were detected by Western blot after lipopolysaccharide(LPS) and Dexamethasone(Dex) treatment of rat alveolar macrophages(AMs),the interaction between BAG-1 and GR determined by immune coprecipitation experiment,and the transcriptional activation of GR measured by relative luciferase activity assay.Results:After LPS and Dex treatment,the expression of BAG-1L in total protein increased but that of BAG-1S remained changed,BAG-1L rather than BAG-1S was detected in nuclear protein and its expression increased gradually within 24 hours,the interaction between BAG-1L and GR was observed in nucleoli,and the transcriptional activation of GR decreased,with a negative correlation between BAG-1L expression and GR activity.Conclusion:LPS and Dex acting on rat alveolar macrophages,the expression of BAG-1L increases,which,coupled with GR,translocates into nucleoli and inhibits GR activity.This might be the important mechanism that underlies glucocorticoid resistance in inflammation.