AIM To evaluate the quality of Gegen Formula Granules.METHODS Linear calibration with two reference substances(LCTRS)was adopted in the predicting of retention time with puerarin and daidzein as internal standards.UPLC characteristic chromatograms were established.The contents of 3'-hydroxy puerarin,puerarin(internal standard),3'-methoxy puerarin,puerarin 6"-O-xyloside,puerarin apioside and daidzin were determined by quantitative determination analysis multi-components by a single marker(QAMS),after which their transfer rates were calculated.RESULTS Compared with relative retention time method,LCTRS demonstrated higher positional accuracy for characteristic peaks and wider application range for columns.There were 9 characteristic peaks in the characteristic chromatograms for 14 batches of formula granules and 15 batches of standard decoctions with the similarities of more than 0.95.The contents and transfer rates of various constituents in formula granules and standard decoctions were basically consistent.CONCLUSION The chemical constituents in formula granules and their standard decoctions of Puerariae lobatae Radix display good consistency,reliable preparation process is observable in the former.

Objective:To study the influencing factors of postpartum post-traumatic stress disorder(PTSD)in pregnant women in the third trimester of pregnancy based on propensity score matching(PSM).Methods:A retrospective analysis was conducted on clinical data of 220 pregnant women in the third trimester who visited our hospital from October 2022 to October 2024,they were divided into PTSD group(n=52)and non-PTSD group(n=168)based on whether PTSD occurred.PSM was adopted to match the age,body mass index(BMI),educational level,average monthly income per capita,natural conception,premature birth,pregnancy complications,first delivery,insomnia,the progress of labor,parenting methods,infant health status and postpartum depression of the two groups at a 1∶1 ratio.After matching,Logistic regression was used to analyze the influencing factors of postpartum PTSD in pregnant women in the third trimester of pregnancy.Result:When comparing the educational level,natural conception,premature birth,pregnancy complications,progress of labor and postpartum depression of the two groups before matching,the differences were statistically significant(P＜0.05).When comparing the comparisons of educational level,natural conception,premature birth,pregnancy complications,progress of labor and postpartum depression of the two groups after matching,the differences were statistically significant(P＜0.05).Logistic regression analysis showed that junior high school education level or below,non-natural conception,premature birth,pregnancy complications,abnormal labor process and postpartum depression were risk factors for PTSD(P＜0.05).Conclusion:Based on PCM,it was found that low educational level,non-natural conception,premature birth,pregnancy complications,abnormal labor process and postpartum depression are risk factors for postpartum PTSD in pregnant women in the third trimester of pregnancy,and reasonable preventive interventions could be taken as early as possible in pregnant women with high risk of PTSD.

Objective:To study the influencing factors of postpartum post-traumatic stress disorder(PTSD)in pregnant women in the third trimester of pregnancy based on propensity score matching(PSM).Methods:A retrospective analysis was conducted on clinical data of 220 pregnant women in the third trimester who visited our hospital from October 2022 to October 2024,they were divided into PTSD group(n=52)and non-PTSD group(n=168)based on whether PTSD occurred.PSM was adopted to match the age,body mass index(BMI),educational level,average monthly income per capita,natural conception,premature birth,pregnancy complications,first delivery,insomnia,the progress of labor,parenting methods,infant health status and postpartum depression of the two groups at a 1∶1 ratio.After matching,Logistic regression was used to analyze the influencing factors of postpartum PTSD in pregnant women in the third trimester of pregnancy.Result:When comparing the educational level,natural conception,premature birth,pregnancy complications,progress of labor and postpartum depression of the two groups before matching,the differences were statistically significant(P＜0.05).When comparing the comparisons of educational level,natural conception,premature birth,pregnancy complications,progress of labor and postpartum depression of the two groups after matching,the differences were statistically significant(P＜0.05).Logistic regression analysis showed that junior high school education level or below,non-natural conception,premature birth,pregnancy complications,abnormal labor process and postpartum depression were risk factors for PTSD(P＜0.05).Conclusion:Based on PCM,it was found that low educational level,non-natural conception,premature birth,pregnancy complications,abnormal labor process and postpartum depression are risk factors for postpartum PTSD in pregnant women in the third trimester of pregnancy,and reasonable preventive interventions could be taken as early as possible in pregnant women with high risk of PTSD.

AIM To evaluate the quality of Gegen Formula Granules.METHODS Linear calibration with two reference substances(LCTRS)was adopted in the predicting of retention time with puerarin and daidzein as internal standards.UPLC characteristic chromatograms were established.The contents of 3'-hydroxy puerarin,puerarin(internal standard),3'-methoxy puerarin,puerarin 6"-O-xyloside,puerarin apioside and daidzin were determined by quantitative determination analysis multi-components by a single marker(QAMS),after which their transfer rates were calculated.RESULTS Compared with relative retention time method,LCTRS demonstrated higher positional accuracy for characteristic peaks and wider application range for columns.There were 9 characteristic peaks in the characteristic chromatograms for 14 batches of formula granules and 15 batches of standard decoctions with the similarities of more than 0.95.The contents and transfer rates of various constituents in formula granules and standard decoctions were basically consistent.CONCLUSION The chemical constituents in formula granules and their standard decoctions of Puerariae lobatae Radix display good consistency,reliable preparation process is observable in the former.

Objective To analyze the peripheral neurotoxicity of utidelone(UTD-1)in patients with human epidermal growth factor receptor 2(HER-2)negative advanced breast cancer and its related factors.Methods The patients with HER-2 negative advanced breast cancer treated by UTD-1 were collected.The patients were divided into control group(low neurotoxicity group,grade 0-1 peripheral neurotoxicity)and treatment group(high neurotoxicity group,grade 2-4 peripheral neurotoxicity)according to cohort method.The data involve age,body mass index(BMI),related indicators of metabolic syndrome(MS)before chemotherapy,hormone receptor expression status of tumor,number of treatment lines,and whether combined with capecitabine were collected.The highest level of neurotoxicity after UTD-1 was collected for each patient,and the possible related factors were analyzed.Results There were 83 cases in the treatment group and 84 cases in the control group.The BMI of treatment and control groups were(24.81±1.93)and(22.82±2.19)kg·m-2,the fasting plasma glucose(FPG)levels were(6.45±1.37)and(5.42±0.79)mmol·L-1,the C-reaction protein(CRP)levels were 11.10 and 1.60 mg·L-1,respectively,and the differences were statistically significant(allP＜0.01).Multi-factor Logistics stepwise regression analysis showed that the high neurotoxicity group was independently correlated with high BMI,high FPG and high CRP(odds ratios were 11.51,7.78 and 6.88).Conclusion BMI,CRP and FPG may be independent risk factors for high peripheral neurotoxicity iduced by UTD-1.

AIM To investigate the consistency of chemical constituents between formula granules and standard decoction of Coptidis Rhizoma.METHODS Eighteen batches of standard decoctions were prepared,after which the extraction rate and contents,transfer rates of magnolflorine,jatrorrhizine,columbamine,epiberberine,coptisine,palmatine,berberin were determined,HPLC characteristic chromatograms were established.RESULTS There were 11 common peaks in the characteristic chromatograms of 18 batches of standard decoctions and 24 batches of formula granules with the similarities of 0.861-1.000,which were clusterd into two categories.The formula granules and standard decoction demonstrated approximated extraction rate and contents,transfer rates of index constituents.CONCLUSION The chemical constituents between formula granules and standard decoction of Coptidis Rhizoma display good consistency,which can provide references for the quality control,process research and clinical application of the former.

Objective To analyze the peripheral neurotoxicity of utidelone(UTD-1)in patients with human epidermal growth factor receptor 2(HER-2)negative advanced breast cancer and its related factors.Methods The patients with HER-2 negative advanced breast cancer treated by UTD-1 were collected.The patients were divided into control group(low neurotoxicity group,grade 0-1 peripheral neurotoxicity)and treatment group(high neurotoxicity group,grade 2-4 peripheral neurotoxicity)according to cohort method.The data involve age,body mass index(BMI),related indicators of metabolic syndrome(MS)before chemotherapy,hormone receptor expression status of tumor,number of treatment lines,and whether combined with capecitabine were collected.The highest level of neurotoxicity after UTD-1 was collected for each patient,and the possible related factors were analyzed.Results There were 83 cases in the treatment group and 84 cases in the control group.The BMI of treatment and control groups were(24.81±1.93)and(22.82±2.19)kg·m-2,the fasting plasma glucose(FPG)levels were(6.45±1.37)and(5.42±0.79)mmol·L-1,the C-reaction protein(CRP)levels were 11.10 and 1.60 mg·L-1,respectively,and the differences were statistically significant(allP＜0.01).Multi-factor Logistics stepwise regression analysis showed that the high neurotoxicity group was independently correlated with high BMI,high FPG and high CRP(odds ratios were 11.51,7.78 and 6.88).Conclusion BMI,CRP and FPG may be independent risk factors for high peripheral neurotoxicity iduced by UTD-1.

The sperm DNA fragmentation index (DFI) is a metric used to assess DNA fragmentation within sperm. During in vitro fertilization-embryo transfer (IVF-ET), high sperm DFI can lead to a low fertilization rate, poor embryo development, early miscarriage, etc. A kinase anchoring protein (AKAP) is a scaffold protein that can bind protein kinase A (PKA) to subcellular sites of specific substrates and protects the biophosphorylation reaction. Sperm protein antigen 17 (SPA17) can also bind to AKAP. This study intends to explore the reason for the decreased fertilization rate observed in high sperm DFI (H-DFI) patients during IVF-ET. In addition, the study investigates the expression of AKAP, protein kinase A regulatory subunit (PKARII), and SPA17 between H-DFI and low sperm DFI (L-DFI) patients. SPA17 at the transcriptional level is abnormal, the translational level increases in H-DFI patients, and the expression of AKAP4/PKARII protein decreases. H2O2 has been used to simulate oxidative stress damage to spermatozoa during the formation of sperm DFI. It indicates that H2O2 increases the expression of sperm SPA17 protein and suppresses AKAP4/PKARII protein expression. These processes inhibit sperm capacitation and reduce acrosomal reactions. Embryo culture data and IVF outcomes have been documented. The H-DFI group has a lower fertilization rate. Therefore, the results indicate that the possible causes for the decreased fertilization rate in the H-DFI patients have included loss of sperm AKAP4/PKARII proteins, blocked sperm capacitation, and reduced occurrence of acrosome reaction.

Abstract: Objective　To analyze the influencing factors of mother-to-child transmission of hepatitis B virus after combined immunological blockade, and to evaluate the effect of mother-to-child blockade, and to provide a basis for health policies and health interventions for preventing mother-to-child blockade of hepatitis B virus. Methods A total of 11 363 pairs of HBsAg positive pregnant women and their infants aged 7-12 months in Hainan Province from 2015 to 2020 were included in the study. The general situation, the situation of health care and delivery in this pregnancy and perinatal period, the detection of hepatitis B markers, the situation of antiviral therapy, the general situation of mother and infant during delivery and the implementation of blockade measures for mother-to-child transmission of hepatitis B were collected and analyzed. Results Among the 11 363 pairs of HBsAg positive pregnant women and their infants delivered in hospitals in Hainan province from 2015 to 2020, the positive rate of HBsAg in children at 7-12 months after birth was 1.47 %, and the difference in HBsAg positive rate of infants born in different years was not statistically significant (P>0.05). There were no significant differences in the positive rate of HBsAg among children born to pregnant women with different nationalities, educational levels, occupations, delivery modes, delivery places, obstetric operations and perineal laceration, abnormal perinatal period, children with different genders and premature delivery and perinatal (all P<0.05). There was significant difference in HBsAg positive rate among infants born to pregnant women of different ages, the positive rate of HBsAg of infants born to young pregnant women was higher than that of older pregnant women (P<0.05). The rate of antiviral therapy was low in HBeAg positive pregnant women, and the positive rate of HBsAg in their infants was 2.54%, which was higher than 0.83% in HBeAg negative pregnant women (P<0.05). Conclusions Combined immunological blockade with hepatitis B vaccine and hepatitis B immunoglobulin can effectively prevent the mother-to-child transmission of HBV. HBsAg-positive women can give birth at the right age, and HBeAg-positive pregnant women can be treated with antiviral therapy to block mother-to-child transmission, providing the important basis for the formulation of hepatitis B prevention and control strategies and measures.

ObjectiveTo investigate the effects of epidural analgesia plus dexmedetomidine infusion on postpartum depression in parturients with natural childbirth. MethodsWe selected 70 parturients aged between 22 and 36， with singleton， term， cephalic presentation， natural delivery and ASA class I or Ⅱ. The cases undergoing epidural analgesia with ropivacaine and sufentanil were randomly divided into two groups by using a random number table （n=35 for each group）. The control group （Group C） used intravenous infusion of normal saline， while the experimental group （Group D） used equivalent volumes of intravenous infusion of dexmedetomidine. Participants were followed up at 1， 6， 12 weeks after childbirth to assess the severity of postpartum depression. Blood samples were collected at 12 h and 48 h after childbirth to measure the serum prolactin levels. The hemodynamic （HR and MAP） changes， VAS scores， and Ramsay scores were recorded at five time points： before analgesia （T1）， 10 min after analgesia （T2）， 30 min after analgesia （T3）， 12 h （T4） and 24 h （T5） after delivery. The number of analgesia pump presses and adverse events were also documented. ResultsCompared with Group C， Group D showed significantly lower EPDS scores at 1 week after childbirth， significantly higher prolactin concentrations at 12 h and 48 h after childbirth， significantly lower VAS scores at T2， T3 and T4， significantly higher Ramsay score at T3 and significantly reduced number of analgesia pump presses （P < 0.05）. ConclusionEpidural analgesia plus intravenous infusion of dexmedetomidine can alleviate early postpartum depression in women undergoing natural delivery， promote early prolactin secretion and provide a safe and effective adjunctive analgesic and sedative effect.