Objective:This study aims to explore the relationship between the single nucleotide polymorphism (SNPs) of Apo liporoteine E (ApoE), GTP cyclohydrolase 1 (GCH1), and J subfamily member of inward rectifier potassium channel-15 (KCNJ15) gene and cognitive dysfunction in Han nationality patients with schizophrenia in Yunan province.Methods:From September 2018 to August 2020, 182 patients with schizophrenia (patient group) and 176 healthy volunteers (control group) were recruited from the Department of Psychiatry and Physical Examination Center respectively, in the First Affiliated Hospital of Kunming Medical University. The Chinese versions of PANSS and the MATRICS Cognitive Battery (MCCB) were used to evaluate psychiatric symptoms and cognitive function respectively. The SNPs of four loci of GCH1 (rs72713460), KCNJ15 (rs928771), and APOE (rs7412 and rs429358) genes were measured by fluorescence in situ hybridization. The differences of genes and genotypes between the two groups were compared. Then multiple linear regression analyses were performed in the patient group. In the regression models, the T scores of each cognitive function test were entered as dependent variables one by one, while the demographic and clinical data and the four SNPs of ApoE, GCH1, and KCNJ15 as independent variables.Results:The T scores of seven cognitive domains of MCCB were lower in the patient group than in the control group ( t=-25.65 to -18.27, all P<0.001). The genotype frequencies of ApoEε3ε4 (55/182 (30.2%) vs. 22/176 (12.5%)) and allele frequencies of ApoEε4 (65/182 (17.9%) vs. 30/176 (8.5%)) were statistically significantly higher in the patient group than in the control group (χ 2=16.64 and 13.55 respectively, both P<0.001). The genotype and allele frequency of GCH1 in patient group were higher than those in control group (χ 2=8.01 to 21.50), and the difference was statistically significant ( P<0.001 or P<0.05). In the patient group, the T scores of 7 cognitive domains were lower in ApoEε4 allele carriers than in non-carriers ( t=4.99 to 17.69), the T scores of 6 cognitive domains were lower in GCH1-T allele carriers than in non-carriers ( t=5.75 to 13.36), and the T scores of 5 cognitive domains were higher in KCNJ15-G allele carriers than in non-carriers ( t=-2.99 to -2.48). In schizophrenia patients, multiple regression analyses showed that the cognitive domains of information processing speed and word learning were related to carrying ApoEε4 and GCH1-T allele. Conclusion:There is pervasive and significant cognitive dysfunction in Han nationality patients with schizophrenia in Yunan province. The polymorphism of ApoE and GCH1 genes may be related to the pathogenesis of cognitive dysfunction in schizophrenia.

Objective:This study aims to explore the relationship between the single nucleotide polymorphism (SNPs) of Apo liporoteine E (ApoE), GTP cyclohydrolase 1 (GCH1), and J subfamily member of inward rectifier potassium channel-15 (KCNJ15) gene and cognitive dysfunction in Han nationality patients with schizophrenia in Yunan province.Methods:From September 2018 to August 2020, 182 patients with schizophrenia (patient group) and 176 healthy volunteers (control group) were recruited from the Department of Psychiatry and Physical Examination Center respectively, in the First Affiliated Hospital of Kunming Medical University. The Chinese versions of PANSS and the MATRICS Cognitive Battery (MCCB) were used to evaluate psychiatric symptoms and cognitive function respectively. The SNPs of four loci of GCH1 (rs72713460), KCNJ15 (rs928771), and APOE (rs7412 and rs429358) genes were measured by fluorescence in situ hybridization. The differences of genes and genotypes between the two groups were compared. Then multiple linear regression analyses were performed in the patient group. In the regression models, the T scores of each cognitive function test were entered as dependent variables one by one, while the demographic and clinical data and the four SNPs of ApoE, GCH1, and KCNJ15 as independent variables.Results:The T scores of seven cognitive domains of MCCB were lower in the patient group than in the control group ( t=-25.65 to -18.27, all P<0.001). The genotype frequencies of ApoEε3ε4 (55/182 (30.2%) vs. 22/176 (12.5%)) and allele frequencies of ApoEε4 (65/182 (17.9%) vs. 30/176 (8.5%)) were statistically significantly higher in the patient group than in the control group (χ 2=16.64 and 13.55 respectively, both P<0.001). The genotype and allele frequency of GCH1 in patient group were higher than those in control group (χ 2=8.01 to 21.50), and the difference was statistically significant ( P<0.001 or P<0.05). In the patient group, the T scores of 7 cognitive domains were lower in ApoEε4 allele carriers than in non-carriers ( t=4.99 to 17.69), the T scores of 6 cognitive domains were lower in GCH1-T allele carriers than in non-carriers ( t=5.75 to 13.36), and the T scores of 5 cognitive domains were higher in KCNJ15-G allele carriers than in non-carriers ( t=-2.99 to -2.48). In schizophrenia patients, multiple regression analyses showed that the cognitive domains of information processing speed and word learning were related to carrying ApoEε4 and GCH1-T allele. Conclusion:There is pervasive and significant cognitive dysfunction in Han nationality patients with schizophrenia in Yunan province. The polymorphism of ApoE and GCH1 genes may be related to the pathogenesis of cognitive dysfunction in schizophrenia.

Objective To investigate the clinical significance of serum CEA , CA19-9, CA72-4, CA242, CA50, PGⅠand PGR ( PGⅠ/PGⅡ) in the Diagnosis of preoperative and postoperative gastric cancer.Methods Retrospective study.The levels of CEA , CA19-9, CA72-4, CA242, CA50, PGⅠand PGⅡin serum of 41 patients with gastric cancer preoperative and postoperative and 60 healthy people were detected by AutoLumo A2000 chemiluminescence immunoassay and compared.Statistical analysis was performed using Rank-sum test by SPSS 17.0.Results The median of CEA, CA19-9, CA72-4, CA242, CA50, PGI, PGII and PGR in postoperative gastric cancer group were 3.79 ng/ml, 17.85 U/ml, 3.50 U/ml, 14.52 U/ml, 17.62 U/ml, 32.81 ng/ml, 11.48 ng/ml, 3.35.The postoperative gastric cancer group were 1.67 ng/ml, 7.76 U ml, 1.73 U/ml, 6.30 U/ml, 7.57 U/ml, 20.56 ng/ml, 5.71 ng/ml, 2.94.The healthy group were 1.53 ng /ml, 7.59 U/ml, 1.47 U/ml, 6.08 U/ml, 5.68 U/ml, 90.86 ng/ml, 14.85 ng/ml, 6.67.There were statistical differences in the serum levels of CEA , CA19-9, CA72-4, CA242, CA50, PGⅠ, PGⅡand PGR among different groups (chi-squared values were 79.108, 20.678, 20.374, 7.252, 56.73, 131.212, 20.38, 86.37, P<0.05).By the Mann-Whitney rank sum test,the serum levels of CEA , CA19-9, CA72-4, CA242 and CA50 in patients with preoperative gastric cancer were significantly higher than those in healthy controls (Z values were -8.598, -4.425, -4.365, -2.000,-7.420, P<0.05).The level of postoperative group was significantly lower than that of preoperative group (Z value were -4.641, -2.383, -2.459, -2.399, -2.903, P<0.05).The serum PGⅠ, PGⅡand PGR levels in patients with preoperative gastric cancer were significantly lower than those in healthy controls (Z values were -10.309, -2.695, 8.637, P<0.05).The PGⅠlevel in the postoperative group was significantly lower than that in the preoperative group (Z value was -2.109, P<0.05).PGⅡ,PGR levels of postoperative group were lower than those of preoperative group , but the difference were not statistically significant.(Z values were -1.506,-0.838, P values were 0.132,0.402).Conclusion The detection of the seven serum markers can help to preoperative diagnosis and postoperative monitoring of gastric cancer .

Objective To investigate the clinical value of 18 F?FDG PET/CT in staging multiple myeloma ( MM) and evaluating the glucose metabolic activity of MM. Methods A total of 25 MM patients ( 13 males, 12 females, age:39-67 years) from May 2010 to April 2015 were enrolled in this retrospective study. The SUVmax of each patient was recorded. D?S plus staging according to 18 F?FDG PET/CT was com?pared with the traditional D?S staging. The SUVmax and the percentage of plasmacytes of bone marrow of phase Ⅲ and non?phase Ⅲ ( phaseⅠand Ⅱ) according to D?S plus staging were compared. Two?sample t test and Wilcoxon rank sum test were used to analyze the data. Results In 25 MM patients, the range of SUVmax of lesions was 1.8-12?0 and the mean value was 5.15±2.74. According to D?S staging, the numbers of patients with phase Ⅰ,Ⅱ andⅢwere 7, 4 and 14, respectively. While the numbers were 3, 1 and 21 by D?S plus staging. Based on the D?S plus staging system, stages of 7 patients ( 28%, 7/25 ) were changed. According to the D?S plus staging system, the SUVmax between phaseⅢand non?phaseⅢpatients was significantly different (5.75±2.54 vs 3.00±0?70; t=2.12, P<0.05), while the percentage of plasma?cytes of bone marrow between the 2 groups had no significant difference ( 17. 50%( 4. 25%-41. 75%) vs 11?15%(10.25%-36.57%);z=0.05, P>0.05). Conclusion 18F?FDG PET/CT is of clinical importance for MM staging and metabolic activity assessment of MM.

Objective To investigate the effects of specific TrkB receptor agonist 7,8-dihydroxyflavone ( 7,8-DHF) on spatial cognitive function and synaptic structure in schizophrenia rat model. Methods SD infant rats were divided into normal control group and model group according to the random number table method on the 6th day after birth. During the postnatal day 7 to 11, rats in the normal control group received subcutaneous injection of 0.9% sodium chloride solution (1 mL·kg-1) twice daily, and the rats in the model group were injected with dizocilpine (0.1 mg·kg-1). Beginning on the postnatal day 60, model rats were randomly divided into 7,8-DHF group and model control group, which were given intraperitoneal injection of 7,8-DHF ( 5 mg·kg-1 ) and DMSO once daily for 14 consecutive days, respectively. The rats of normal control group were given equal volume injections of DMSO. Morris water maze task, Golgi staining and Western blotting were adopted to examine spatial cognitive function, hippocampal dendritic spine density, protein expression and activity, respectively. Results The result in the open field test showed that the total travelled distance within 5 min was (12.20±1.62) m in the normal control group, (11.73±1.36) m in the model control group and (12.94±1.09) m in the 7,8-DHF group. The escape latency and travelled distance in the model control group were significantly higher than those in the normal control group (P<0.05), and the escape latency and travelled distance in rats of 7,8-DHF group were significantly shortened as compared with those in the model control group (P<0.05). There was no significant difference in the swimming speed among the three groups (P>0.05). The hippocampal dendritic spine density was (14.2±2.3)/10 μm in the normal control group, (8.0±1.9)/10 μm in the model control group, and (13.5±1.7)/10 μm in the 7,8-DHF group, the differences between the three groups were significant ( all P<0.05);the phosphorylation level of GluR1 protein was (100.0±5.0) in the normal control group, (47.9±10.8) in the model control group, and (97.5±9.3) in the 7,8-DHF group, and the differences among the three groups were significant ( all P<0. 05 ) . Conclusion 7, 8-DHF treatment could improve the spatial cognitive function in rat model of schizophrenia and the mechanisms might be related with the increases of hippocampal dendritic spine density and phosphorylated levels of GluR1.

BACKGROUND:Currently, the enzymatic digestion combined with magnetic activated cel sorting for isolating microvascular endothelial cel s are cumbersome and do harm to cel s. Therefore, how to simplify the isolation and culture of human dermal microvascular endothelial cel s to obtain highly purified endothelial cel s in vitro becomes a hotspot.
OBJECTIVE:To explore a simple and effective cultivation method of microvascular endothelial cel s from diabetic patient skins in vitro, and to detect the cel growth.
METHODS:Diabetic patients with chronic foot wounds after amputation were enrol ed to col ect the limb proximal skin and topical skin around the wound superficial dermal tissue. Human dermal microvascular endothelial cel s were obtained using adherent method and trypsin method, fol oewd by purified utilizing trypsin digestion and repeated attachment method when passage culture.
RESULTS AND CONCLUSION:Human dermal microvascular endothelial cells were obtained successfully, Primary cultured endothelial cells completely adhered to the wall at 24 hours, entered the logarithmic phase at the 10th day, and the cell concentration reached 80%at the 12th-13th day. While the passage cells grew more actively than primary cells, and fully covered the bottom in a“cobblestone”arrangement after 5-7 days of culture. Immunohistochemical staining showed that cultured cells were positive for FVIII and CD31-associated antigens with 100%positive rate. MTT assay showed that cell growth curves of 2, 4, and 5 generations of dermal microvascular endothelial presented the invertedSshape. These results suggest that abundant highly purified human dermal microvascular endothelial cells can be obtained through the adherent method and a small amount of short-term trypsin method.

OBJECTIVETo determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).

METHODSMixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.

RESULTSMost of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.

CONCLUSIONGenetic reassortment can occur between Hantaan virus and Seoul virus strains.

Animals ; Cercopithecus aethiops ; Genome, Viral ; Genotype ; Hantaan virus ; genetics ; RNA, Viral ; genetics ; Reassortant Viruses ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Seoul virus ; genetics ; Vero Cells