Objective: To investigate the health literacy level and its influencing factors among the elderly in nursing homes in Xiangshan County, Zhejiang Province, so as to provide the reference for improving the health literacy of the elderly in nursing homes. Methods: In September to November 2024, elderly individuals aged ≥60 years from nursing homes in Xiangshan County were selected by a stratified random sampling method. Data on basic information and health literacy were collected using the Chinese Elderly Health Literacy Questionnaire. The health literacy level of the elderly in nursing homes was analyzed. The influencing factors of their health literacy level was analyzed using a multivariable logistic regression model. Results: Among the surveyed participants, there were 265 males (41.09%) and 380 females (58.91%). The median age was 83.00 (interquartile range, 12.00) years. A total of 42 individuals had health literacy, giving a health literacy level of 6.51%. The health literacy levels of basic concepts, basic skills and basic knowledge were 15.04%, 15.04% and 5.74%, respectively. The health literacy levels of health service utilization and chronic disease management, scientific view of aging, information acquisition and self-care, healthy lifestyle, intrinsic capacity and geriatric syndromes, and safety and first aid, were 49.15%, 39.22%, 24.81%, 20.78%, 11.63% and 1.71%, respectively. Multivariable logistic regression analysis showed that gender (female, OR=3.720, 95%CI: 1.687-8.203), age (70-<80 years old, OR=0.084, 95%CI: 0.016-0.428), educational level (junior high school, OR=5.690, 95%CI: 1.433-22.600; senior high school/vocational high school/technical secondary school and above, OR=14.620, 95%CI: 3.321-64.361), marital status (unmarried/divorced/widowed, OR=0.263, 95%CI: 0.122-0.567), and personal monthly income (1 000-<3 500 yuan, OR=0.136, 95%CI: 0.024-0.763) were factors affecting the health literacy level of the elderly in nursing homes. Conclusions The health literacy level among the elderly in nursing homes in Xiangshan County are relative low in terms of basic knowledge, intrinsic capacity and geriatric syndromes, and safety and first aid. Gender, age, educational level, marital status, and monthly income are influencing factors for health literacy level among the elderly in nursing homes.

Purpose To investigate the expression pattern of podoplanin in the periglandular fibroblasts and cancer-associ-ated fibroblasts of early-stage colorectal cancer,to analyze its re-lationship with clinicopathological features,and to explore its significance in clinicopathological diagnosis.Methods A total of 166 cases of early-stage colorectal cancer were enrolled.The deepest invasion tissue samples from 166 cases were explored with immunohistochemistry for expression profiling of podopla-nin.According to the expression pattern of analyze in the stroma,Pattern A showed that the periglandular fibroblasts were pole-ori-ented and uniformly expressed podoplanin,and stronger than CAFs around the glands,periglandular fibroblasts completely sur-round the glands;Pattern B showed that the periglandular fibro-blasts were loss of cell polarity,and the staining intensity was less than or equal to CAFs around the glands.And the correlation was also analyzed between the two expression patterns and clini-copathological features.Results Among 166 cases,55 cases were the Pattern A of podoplanin expression and 111 cases were the Pattern B.Monofactor analysis showed statistically significant differences in lymphatic vessel invasion,vascular invasion,bud-ding grade,differentiation degree,infiltration pattern and Kiku-chi classification between the two groups(P＜0.05).Multivari-ate analysis identified that infiltration pattern was an independent factor between the two groups(P＜0.05).Conclusion Podo-planin expression patterns are different in the stroma of early colorectal cancer and correlated with risk factors for lymph nodes metastasis.In clinicopathological diagnosis,podoplanin can be used as an auxiliary diagnostic tool to improve the consistency of pathologists'judgment of infiltration forms.

Purpose To investigate the expression pattern of podoplanin in the periglandular fibroblasts and cancer-associ-ated fibroblasts of early-stage colorectal cancer,to analyze its re-lationship with clinicopathological features,and to explore its significance in clinicopathological diagnosis.Methods A total of 166 cases of early-stage colorectal cancer were enrolled.The deepest invasion tissue samples from 166 cases were explored with immunohistochemistry for expression profiling of podopla-nin.According to the expression pattern of analyze in the stroma,Pattern A showed that the periglandular fibroblasts were pole-ori-ented and uniformly expressed podoplanin,and stronger than CAFs around the glands,periglandular fibroblasts completely sur-round the glands;Pattern B showed that the periglandular fibro-blasts were loss of cell polarity,and the staining intensity was less than or equal to CAFs around the glands.And the correlation was also analyzed between the two expression patterns and clini-copathological features.Results Among 166 cases,55 cases were the Pattern A of podoplanin expression and 111 cases were the Pattern B.Monofactor analysis showed statistically significant differences in lymphatic vessel invasion,vascular invasion,bud-ding grade,differentiation degree,infiltration pattern and Kiku-chi classification between the two groups(P＜0.05).Multivari-ate analysis identified that infiltration pattern was an independent factor between the two groups(P＜0.05).Conclusion Podo-planin expression patterns are different in the stroma of early colorectal cancer and correlated with risk factors for lymph nodes metastasis.In clinicopathological diagnosis,podoplanin can be used as an auxiliary diagnostic tool to improve the consistency of pathologists'judgment of infiltration forms.

Objective To identify Mobiluncus species with matrix-assisted laser desorption/ioniza-tion time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing and to analyze anti-biotic resistance genes and phylogenetic relationships among Mobiluncus species with whole genome sequen-cing. Methods Twenty-five Mobiluncus strains were isolated after anaerobic culture of 65 vaginal secretion samples of patients with bacterial vaginosis (BV) and identified to species level by MALDI-TOF MS and 16S rRNA gene sequencing. The whole genome DNA of each strain was extracted for next-generation sequencing. SPAdes was used to assembly genomes. Resistance genes were searched in ARGD database. The core ge-nome of all isolates was analyzed by Harvest to construct phylogenetic tree. Results MALDI-TOF MS could only identify Mobiluncus curtisii, while 16S rRNA gene sequencing could identify both Mobiluncus mulieris and Mobiluncus curtisii. Results of the whole genome sequencing showed that tetracycline resistance gene tet ( o) and macrolides resistance gene erm(x) were the two predominant acquired resistance genes of Mobilun-cus with a positive rate of 84. 7% and 61. 5% respectively. Intra-species relationships of the two Mobiluncus species were close, but a distant phylogenetic relationship was found between the two species. Conclusion This study shows that MALDI-TOF MS can't be used to identify Mobiluncus mulieris at present. Mobiluncus strains have potential resistance to tetracycline and macrolides. Intra-species evolution of Mobiluncus is slow, which indicates that there is no growing trend towards new species for the time being.