Objective To analyze the auxiliary diagnostic effect of oral cone beam computed tomography(CBCT)in root canal treatment of flight personnel.Methods Eighty flight personnel who underwent root canal treatment in Qingdao Special Service Rehabilitation Center of the Navy from February 2020 to February 2022 were enrolled in this study.All the patients received X-ray examination and oral CBCT.The number of detected root canals,root canal localization,and root canal treatment were analyzed.Results There were 235 root canals in the 80 patients.The detection rate of oral CBCT was significantly higher than that of X-ray examination(94.47%vs 87.66%,P<0.05).A total of 206 root canals were detected by both detection methods,and the Kappa value for consistency in the number of root canals detected was 0.643(P<0.05).The successful rate of root canal negotiation assisted by oral CBCT was significantly higher than that of X-ray examination(90.64%vs 82.98%,P<0.05).Conclusion Oral CBCT can effectively assist in the detection of complex root canals,increase the number of detected root canals and assist in the location of the root canals,check the calcification of root canals,and guide root canal negotiation,which provide a guarantee for complex root canal treatment of flight personnel.

Objective : To explore the mechanism of isorhamnetin (Iso) in the treatment of oral squamous cell carcinoma (OSCC) using network pharmacology and molecular docking methods and to verify it in vitro. Methods : The key targets were obtained by constructing the PPI protein interaction network based on the common intersection targets of Iso-OSCC. At the same time, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) were used to analyze the related signaling pathways of the intersection targets. Iso and core targets were also analyzed through molecular docking and visualization. Colony formation assay and Transwell assay were used to identify the effect of Iso on the proliferation and invasion of Cal-27 cells. Western blot was used to analyze the regulatory effects of different concentrations of Iso on estrogen receptor-1 (ESR1), phosphoinositide-3-kinase regulatory subunit-1 (PIK3R1), Src tyrosine kinase (SRC), and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway proteins. Results: A total of 269 potential intersection targets of Iso-regulated OSCC were obtained. According to the degree obtained by topological analysis, PIK3R1, AKT1, SRC, ESR1, and other core targets were screened out. KEGG analysis showed that 165 signaling pathways were enriched in the intersection targets of Iso-OSCC, among which the PI3K/AKT signaling pathway played an important role in the treatment of OSCC with Iso. Molecular docking results showed that the absolute value of binding energy between target proteins PIK3R1, AKT1, SRC, ESR1, and Iso was high. After Cal-27 cells were treated with Iso, the number of cell colony formations, the number of transmembrane cells, and the expression of PIK3R1, ESR1, SRC, p-PI3K, and p-AKT were negatively correlated with the increase in Iso concentration (P < 0.05). Conclusion Iso can inhibit PI3K/AKT signal transduction and influence the expression of PIK3R1, AKT1, SRC, and ESR1 proteins, thereby inhibiting the occurrence and development of OSCC.

CRISPR-Associated Protein 9 ; Gene Editing ; CRISPR-Cas Systems/genetics*

Objective:To observe the clinical effect of modern root canal therapy on pulpitis in flying personnel.Methods:A total of 229 patients who were diagnosed as pulpitis from March of 2018 to April of 2020 were divided into 2 groups alternately according to the order of treatment. One hundred and fifteen patients in the study group that received modern root canal therapy and 114 patients in the control group that received the conventional root canal therapy. The clinical symptoms (red and swollen gums, pain and chewing discomfort) score, visual analogue scale (VAS) and masticatory ability score were respectively investigated before treatment and 2 weeks after treatment. The quality of life (QOL) score was investigated before treatment and 1 month after treatment. The total effective rate was evaluated 2 weeks after treatment and the incidences of adverse reactions (local swelling, root canal obstruction and occlusive pain) were analyzed. The recurrence rates by 6-month treatment and the treatment results were compared between 2 groups.Results:The total effective rate of the study group was 93.04%, which was higher than that of the control group (84.21%), and the difference between the 2 groups was statistically significant ( χ2=4.438, P=0.035). There were no differences on clinical symptoms score, QOL score, VAS, inflammatory index and masticatory ability score between 2 groups before treatment (all P>0.05). The clinical symptoms score, VAS, the levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in the study group after treatment were lower than those of the control group, but the masticatory ability score and QOL score were higher than those of the control group after treatment. The differences between the 2 groups were statistically significant ( t=8.229-46.504, all P=0.001). The incidence of adverse reactions and recurrence rate in the study group was 7.83% and 5.22% respectively, which was lower than that of the control group (17.54% and 13.16%, respectively, χ2=4.881, 4.334, P=0.027, 0.037). Conclusions:The modern root canal therapy has better clinical effect on the treatment of pulpitis. It can shorten the treatment courses, reduce the inflammation response, pain degree, adverse reactions and recurrence rate, improve the curative rate and the life of teeth of flying personnel, and reduce the adverse factors in high altitude flight.

Objective:To observe the clinical effect of modern root canal therapy on pulpitis in flying personnel.Methods:A total of 229 patients who were diagnosed as pulpitis from March of 2018 to April of 2020 were divided into 2 groups alternately according to the order of treatment. One hundred and fifteen patients in the study group that received modern root canal therapy and 114 patients in the control group that received the conventional root canal therapy. The clinical symptoms (red and swollen gums, pain and chewing discomfort) score, visual analogue scale (VAS) and masticatory ability score were respectively investigated before treatment and 2 weeks after treatment. The quality of life (QOL) score was investigated before treatment and 1 month after treatment. The total effective rate was evaluated 2 weeks after treatment and the incidences of adverse reactions (local swelling, root canal obstruction and occlusive pain) were analyzed. The recurrence rates by 6-month treatment and the treatment results were compared between 2 groups.Results:The total effective rate of the study group was 93.04%, which was higher than that of the control group (84.21%), and the difference between the 2 groups was statistically significant ( χ2=4.438, P=0.035). There were no differences on clinical symptoms score, QOL score, VAS, inflammatory index and masticatory ability score between 2 groups before treatment (all P>0.05). The clinical symptoms score, VAS, the levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in the study group after treatment were lower than those of the control group, but the masticatory ability score and QOL score were higher than those of the control group after treatment. The differences between the 2 groups were statistically significant ( t=8.229-46.504, all P=0.001). The incidence of adverse reactions and recurrence rate in the study group was 7.83% and 5.22% respectively, which was lower than that of the control group (17.54% and 13.16%, respectively, χ2=4.881, 4.334, P=0.027, 0.037). Conclusions:The modern root canal therapy has better clinical effect on the treatment of pulpitis. It can shorten the treatment courses, reduce the inflammation response, pain degree, adverse reactions and recurrence rate, improve the curative rate and the life of teeth of flying personnel, and reduce the adverse factors in high altitude flight.

"The Expert Group on Tumor Ablation Therapy of Chinese Medical Doctor Association, The Tumor Ablation Committee of Chinese College of Interventionalists, The Society of Tumor Ablation Therapy of Chinese Anti-Cancer Association and The Ablation Expert Committee of the Chinese Society of Clinical Oncology" have organized multidisciplinary experts to formulate the consensus for thermal ablation of pulmonary subsolid nodules or ground-glass nodule (GGN). The expert consensus reviews current literatures and provides clinical practices for thermal ablation of GGN. The main contents include: (1) clinical evaluation of GGN, (2) procedures, indications, contraindications, outcomes evaluation and related complications of thermal ablation for GGN and (3) future development directions.
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Objective:To prepare and characterize chitosan/hyaluronic acid (CS/HA) nanoparticles for miRNA-148b (miR-148b)delivery.Methods:CS/HA/miR-148b nanoparticles were prepared.The particle size,zeta potential,morphology were investigated by nano laser granulometer and TEM respectively.The encapsulation efficiency of CS/HA nanoparticles was evaluated by gel retarding analysis.The cytotoxicity and transfection efficiency of CS/HA/miR-148b nanoparticles on rat bone marrow mesenchymal stem cells (MSCs) were evaluated by CCK-8 assay,transfection assay and FCM respectively.Results:The CS/HA/miR-148b nanoparticles were discrete spherical particles with the diameter of 160 to 370 nm and zeta potential of + 15 to +41 mV.N/P ratio at 20∶1 of the nanoparticles showed the optimum encapsulation efficiency for miR-148b.CS/HA/miR-148b nanoparticles exhibited no cytotoxicity to MSCs and could deliver miR-148b to the MSCs at high transfection efficiency.Conclusion:CS/HA/miR-148b nanoparticles are safe and effective for miR-148b delivery into MSCs.

BACKGROUND: Tissue engineering is a booming subject in recent years, involving skin, cornea, bone, cartilage, tendon and etc. Noticeably, tissue-engineered skin is an issue of concern, which provides an alluring prospect for the repair of skin defects. OBJECTIVE: To overview the latest research progress of tissue-engineered skin. METHODS: A computer-based research of Medline database from 1996 to 2017 was performed, with the English keywords of epidermis, dermis, tissue engineered skin. The latest research status of tissue-engineered skin was summarized in views of epidermal and dermal substitutes, followed by exploring the problems and prospects of tissue-engineered skin. RESULTS AND CONCLUSION: A total of 65 literatures were included. Complex skin substitutes include epidermis and dermis, in which hair follicles, sweat glands, sebaceous glands and other cutaneous appendages are absent. Therefore, how to solve above problems is a hotspot issue and major trend in tissue-engineered skin. Tissue-engineered skin aims to construct permanent skin substitutes containing melanocytes and Langerhans cells rapidly. Since the current tissue-engineered skin substitutes can completely replace skin tissues neither in morphology nor in function, in-depth studies are expected in the near future.

Objective:To evaluate the effects of magnetron-sputtered ZrSiN/ZrO2 gradient coating on the bonding strength of commercially pure titanium to porcelain.Methods:50 cast titanium specimens were prepared according to the ISO 9693 standard,and divided into 2 groups(n =25).The ZrSiN/ZrO2 gradient coating was deposited on specimens by magnetron sputtering technique,and subsequently a low-fusing porcelain was applied for the samples in the expermental group,the samples in the control group were treated by surface sandblasting.The roughness and surface energy of the samples were measured(n =10).The bonding strength of titanium-porcelain specimens was analyzed by three-point bending test (n =10).Scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDS) were employed to examine the properties of the delaminated and cross-sectioned surfaces.The results were analyzed by paired t-test (α =0.05) with SPSS 1 1.0 software.Results:The roughness of the experimantal group was lower(P ＜ 0.05) and the contact angle was larger(P ＜ 0.05) than those in the control group.The titanium-porcelain bond strength of gradient-coated group was greater than that of control group(P ＜0.05).The SEM and EDS examination results of delaminated and cross-sectioned surfaces also indicated that the gradient-coated group showed more porcelain residues.Conclusion:The bonding strength of porcelain-fused-to-titanium can be improved by magnetron sputtered ZrSiN/ZrO2 gradient coating.

SUMMARY Thehumanembryonicstemcells(hESCs)serveasaself-renewable,genetically-healthy, pluripotent and single source of all body cells,tissues and organs.Therefore,it is considered as the good standard for all human stem cells by US,Europe and international authorities.In this study,the standard and healthy human mesenchymal progenitors,ligament tissues,cardiomyocytes,keratinocytes,primary neurons,fibroblasts,and salivary serous cells were differentiated from hESCs.The human cellular health-safety of NaF,retinoic acid,5-fluorouracil,dexamethasone,penicillin G,adriamycin,lead ace-tate PbAc,bisphenol A-biglycidyl methacrylate (Bis-GMA)were evaluated selectively on the standar-dized platforms of hESCs,hESCs-derived cardiomyocytes,keratinocytes,primary neurons,and fibro-blasts.The evaluations were compared with those on the currently most adopted cellular platforms.Parti-cularly,the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial cells Beas-2B and human umbilical vein endo-thelial cells (HUVECs)were evaluated.The results showed that the standardized hESCs cellular plat-forms provided more sensitivity and accuracy for human cellular health-safety evaluation.