OBJECTIVE To investigate the clinical characteristics of Haemophilus influenzae strains isolated from respiratory trace specimens of the hospitalized adult patients with pneumonia and analyze the change of drug resist-ance from 2022 to 2024.METHODS The clinical data and drug susceptibility testing data for the H.influenzae strains isolated from respiratory tract specimens were collected from the 1 462 adult patients with pneumonia who were hospitalized in Foshan Sanshui District People's Hospital from Jan.2022 to Dec.2024.The clinical character-istics of the patients with H.influenzae infection and the change of drug resistance were retrospectively analyzed with the use of SPSS 22.0 and WHONET 5.6 software.RESULTS Totally 223 of 1462 hospitalized patients were diagnosed with H.influenzae infection,the detection rate of H.influenzae was 16.38％among the male pa-tients,higher than 12.00％among the female patients,and there was significant difference(P＜0.05);the de-tection rate was higher in summer and autumn(P＜0.05).The patients with chronic obstructive pulmonary dis-ease accounted for 34.08％.The result of drug susceptibility testing for the 223 strains of H.influenzae showed that the positive rate of β-lactamase was 63.23％,and the isolation rate of β-lactamase-negative ampicillin-resistant strains was 9.86％.There was significant difference in the drug resistance rate to azithromycin among the H.in-fluenzae strains isolated from 2022 to 2024(x2=6.715,P=0.035),and the drug resistance rate of the H.in-fluenzae strains to azithromycin was higher in 2024(40.77％).CONCLUSIONS The male patients and the patients with COPD are dominant among the hospitalized adult pneumonia patients with H.influenzae infection,which is prevalent in spring and winter.The drug resistance analysis shows that the positive rate of β-lactamase and the drug resistance rate to ampicillin are high,and it should be aware of the empirical use of penicillin.The drug re-sistance rate to azithromycin is increased year by year;the multidrug-resistance in the H.influenzae strains may pose new challenge to the clinical diagnosis and treatment.

OBJECTIVE To investigate the clinical characteristics of Haemophilus influenzae strains isolated from respiratory trace specimens of the hospitalized adult patients with pneumonia and analyze the change of drug resist-ance from 2022 to 2024.METHODS The clinical data and drug susceptibility testing data for the H.influenzae strains isolated from respiratory tract specimens were collected from the 1 462 adult patients with pneumonia who were hospitalized in Foshan Sanshui District People's Hospital from Jan.2022 to Dec.2024.The clinical character-istics of the patients with H.influenzae infection and the change of drug resistance were retrospectively analyzed with the use of SPSS 22.0 and WHONET 5.6 software.RESULTS Totally 223 of 1462 hospitalized patients were diagnosed with H.influenzae infection,the detection rate of H.influenzae was 16.38％among the male pa-tients,higher than 12.00％among the female patients,and there was significant difference(P＜0.05);the de-tection rate was higher in summer and autumn(P＜0.05).The patients with chronic obstructive pulmonary dis-ease accounted for 34.08％.The result of drug susceptibility testing for the 223 strains of H.influenzae showed that the positive rate of β-lactamase was 63.23％,and the isolation rate of β-lactamase-negative ampicillin-resistant strains was 9.86％.There was significant difference in the drug resistance rate to azithromycin among the H.in-fluenzae strains isolated from 2022 to 2024(x2=6.715,P=0.035),and the drug resistance rate of the H.in-fluenzae strains to azithromycin was higher in 2024(40.77％).CONCLUSIONS The male patients and the patients with COPD are dominant among the hospitalized adult pneumonia patients with H.influenzae infection,which is prevalent in spring and winter.The drug resistance analysis shows that the positive rate of β-lactamase and the drug resistance rate to ampicillin are high,and it should be aware of the empirical use of penicillin.The drug re-sistance rate to azithromycin is increased year by year;the multidrug-resistance in the H.influenzae strains may pose new challenge to the clinical diagnosis and treatment.

Objective To investigate the effects of Rosa Laevigata Michx Flavoid( RLMF) and Rosa laevigata Michx Polysaccharose (RLMP) on expression of TRPV5 in IgA Nephropathy (IgAN) rat renal tissue. Methods Forty Sprague-Dawley rats were randomly assigned to four groups.The rat model of IgA nephropathy was induced by intragastric administration of bovine serumalbumin and injections of LPS and CC14.Eight weeks later,the rats with IgAN were treated with RLMF or RLMP (4 weeks), or normal saline.Rats was sacrificed at thirteenth weeks, and RNA was extracted from the kidney.Expression of TRPV5 in tubulointerstitial tissues were analyzed by fluorescent quantitative RT-PCR. Results After RFLP intervention,the expression levels of TRPV5 were markedly increased (P<0.01) than model control group,while decreased (P<0.05) than normal control group but had no significance with model control group after RFLF intervention. Conclusion TRPV5 expression is decreased in IgAN,and RLMP can adjust TRPV5 expression and improve renal function of IgAN.

To construct a scFv antibody mini-library by phage display technique from the spleen cells of BALB/c mice immunized with extracellular domain of N-cadherin (N-cad), CD34 and AC133, the extracellular domain genes of N-cad, CD34 and AC133 were cloned into a phagemid pSEX81 respectively, and were then displayed on the phagemid in the form of fusion protein with p III protein. After being effectively immunized with the fusion protein, the spleen of the mice were harvested, and total RNA were extracted from the spleen. The cDNA of VH and VL genes were amplified by RT-PCR, and a scFv-phage display antibody library was constructed with the amplified V-genes. The content, multiplicity and expressing potential of the library were examined. As a result, we had produced a scFv library containing 1.4 x 10(6) individual clones which showed different patterns after being digested with restriction endoneuclease Mua I. The surface display expression of the library was also verified. It indicated that the capacity and diversity of the library was sufficient for screening specific scFv antibody against N-cad, CD34 and AC133. The library will be useful for isolating corresponding specific scFv antibodies.
AC133 Antigen ; Animals ; Antibodies ; genetics ; Antigens, CD ; immunology ; Antigens, CD34 ; immunology ; Base Sequence ; Cadherins ; immunology ; Female ; Glycoproteins ; immunology ; Immunoglobulin Fragments ; biosynthesis ; genetics ; Immunoglobulin Variable Region ; biosynthesis ; genetics ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Peptide Library ; Peptides ; immunology

Animals ; Blood Platelets ; cytology ; Bone Marrow Cells ; cytology ; Cell Differentiation ; Cells, Cultured ; Culture Media, Conditioned ; Cyclophosphamide ; Female ; Fibroblasts ; cytology ; Male ; Megakaryocytes ; cytology ; Mice ; Stem Cells ; cytology ; Thrombocytopenia ; chemically induced ; therapy

OBJECTIVETo investigate telomerase activity and expression of hTR and hTERT in human neuroepithelial tumors for exploring new strategy for clinical diagnosis and treatment.

METHODSTelomerase activity was detected by modified TRAP method and the expression of hTR and hTERT was measured by RT-PCR method in 65 human neuroepithelial tumors, respectively.

RESULTSThe positive rates of telomerase and hTERT were 61.54% and 70.77% respectively in human neuroepithelial tumors, and the positive rate and their level of expression were correlated with the degree of malignancy of tumors positively.

CONCLUSIONSTelomerase activity and hTERT are significantly correlated with the degree of malignancyin human neuroepithelial tumors. hTERT may play a key role in the regulation of telomerase activity.

DNA-Binding Proteins ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Humans ; Neoplasms, Neuroepithelial ; enzymology ; genetics ; Telomerase ; biosynthesis ; genetics ; metabolism

AIM: To clone the extracellular domain of N-cadherin cDNA, and to observe the antigenicity of the expressed protein. METHODS: Total RNA was extracted from CD34+ cells separated from fetal liver and bone marrow cells. The extracellular domain of N-cad cDNA was amplified with RT-PCR and inserted into a vector pOPE101-215. The recombinant pOPE-N-cad was expressed with IPTG induction. Then, mice were immunized with the protein. RESULTS: The extracellular domain of N-cad cDNA from CD34+ cells was identified by DNA sequencing. The recombinant pOPE-N-cad in host XL1-blue expressed a 70 kD protein after induced with IPTG, and anti-N-cad antibody was detected in serum of the immunized mice after 5 times injection of the recombinant N-cad protein. CONCLUSION: CD34+ cells bore N-cad gene and the recombinant protein of the extracellular domain of N-cad cDNA shows good immunogenicity.