Objective To investigate the expression of endothelin-1(ET-1)and placental growth factor(PLGF)in patients with preeclampsia in early pregnancy and its prognostic significance.Methods A total of 89 patients with preeclampsia who were admitted to No.904 Hospital of Joint Logistic Support Force of PLA from March 2019 to May 2021 were assigned to observation group.And 81 healthy pregnant women who underwent physical examination during the same period were enrolled as controls.Their clinical data were retrospectively analyzed.Serum ET-1 and PLGF levels were detected after admission.The correlation between serum ET-1 and PLGF levels was analyzed.All the participants were followed up until term pregnancy,and the prognoses of the two groups were compared.The patients in the observation group were subdivided into good prognosis group and poor prognosis group according to the prognoses.Multivariate logistic regression analysis was used to analyze the risk factors of poor prognosis in patients with preeclampsia.The receiver operating characteristic(ROC)curve was prepared,and the area under the curve(AUC)was used to evaluate the predictive value of serum ET-1 and PLGF levels for poor prognosis of preeclampsia patients.Results Serum ET-1 level in the observation group was higher than that in the control group,while serum PLGF level in the observation group was lower than that in the control group(both P<0.05).Pearson correlation analysis showed that there was a negative correlation between serum ET-1 and PLGF in both groups(P<0.05).The incidence of poor prognosis in the observation group was significantly higher than that in the control group(20.22%vs 7.41%,P<0.05).The proportion of severe cases,24-hour urine protein quantitation,uric acid,and serum ET-1 in the poor prognosis group were higher than those in the good prognosis group,while serum PLGF level in the poor prognosis group was lower than those in the good prognosis group(P<0.05).Multivariate logistic regression analysis showed that severe condition,24-hour urine protein quantitation,serum ET-1 and PLGF levels were risk factors for poor prognosis of preeclampsia patients(all P<0.05).The ROC curve showed that the AUC(95%CI)values of serum ET-1 and PLGF in predicting poor prognosis of preeclampsia were 0.825(0.709-0.906)and 0.771(0.651-0.852),respectively.Conclusion Patients with preeclampsia have higher ET-1 and lower PLGF than healthy pregnant women.Both ET-1 and PLGF can be used to predict the prognosis of patients with preeclampsia.

In order to figure out phylogenetic relationship and genetic diversity of different geographical populations,genetic analyses of Aedes albopictus were performed based on mitochondrial gene COI.Based on samples collected from most distribution regions in China,mitochondrial gene Cytochrome C Oxidase Subunit I was obtained through PCR and DNA sequence.Together with some COI sequences downloaded from GenBank,60 COI sequences with the final length of 598 bp were used for subsequent analyses.Results showed that there was no obvious divergence according to phylogenetic analyse,all sequences were clustered together in Maximum Likelihood tree.Sixteen haplotypes were detected,and four of them shared haplotypes.Haplotype diversity (Hd) was 0.737,nucleotide diversity (π) was 0.20 ％.Population genetic differentiation analyses demon strated that Hainan population showed obvious divergences.In the network of haplotypes,H1 and H6 was found to be the primary haplotypes,and they formed two radical centers.All these results indicate that A.albopictus populations of China are expanding presently,and Hainan population become differential with other geographical populations,which probably attribute to geographical isolations.

Objective To investigate the effects of Shuxuetong Injection (SXT ) on expressions of cell apoptosis and TLR4 a-round ischemic area after focal cerebral infarction in rats and to discuss its neuroprotective mechanism on ischemia-induced brain in-jury .Methods The SD rats were subjected to establish the model of middle cerebral artery occlusion (MCAO)by nylon monofila-ment suture ,then were randomly divided into the sham-operated group ,the model group and the SXT treatment group ;the cell ap-optosis and the expressions of TLR4 mRNA and protein around ischemic area at 12 ,24 ,48 ,72 h after cerebral ischemia were detec-ted respectively by TUNEL test mediated with DNA ,RT-PCR and immunohistochemistry .Results In the model group ,the number of TUNEL positive cells ,the expressions of TLR4 mRNA and protein were gradually increased at 12 h ,reached the peak at 24 h , then decreased and were still higher than those in the sham-operated group(P<0 .01);in the SXT treatment group ,these expres-sions after 24 h were lower than those in the model group (P<0 .05)and declined as the treatment time increase(P<0 .05) .Conclu-sion In subacute stage of cerebral ischemia injury ,apoptosis is related with the expression of TLR4 ,SXT may inhibit apoptosis , down-regulate the expression of TLR4 around ischemic area ,this may be one of the mechanisms of neuroprotection .

Objective To compare the effects of electroacupuncture (EA) at different acupoints on the neuroethology and expression of neurocan after focal cerebral infarction in rats. Methods The healthy male adult Sprague-Dawley rats were randomly divided into sham group (n=5), model group (n=20), Shuigou-Baihui (SB) group (n=20), Ganshu-Shenshu (GS) group (n=20) and Zusanli-Quchi (ZQ) group.All the rats were modeled with middle cerebral artery occlusion (MCAO) except sham group, and were randomly divided into 5 sub-groups as 1 d, 3 d, 7 d, 14 d and 21 d after cerebral ischemia. Their neuroethology was evaluated with Longa's score, and the expressions of neurocan mRNA and protein were detected with RT-PCR and immunohistochemistry in ischemic cortex. Results No dysfunction was found in the sham group. The neurological function recovered gradually 3 d after modeled, and improved significantly 7~21 d after modeled in each EA group compared with those in the model group (P<0.05). The expression of neurocan mRNA and protein gradually increased 1 d after modeled in the model group, peaked 14 d after modeled, and remained high level 21 d after modeled (P<0.01). The expressions of neurocan mRNA and protein were less in each EA group than in the model group 3 d after modeled (P<0.05), and were less in the SB group and GS group than in ZQ group (P<0.05). Conclusion The expression of neurocan increased significantly in ischemic cortex. EA may down-regulate the expression of neurocan after acute cerebral infarction, and improve the neuroethology. EA at SB and GS is more effective than at ZQ.