Purpose To investigate the patterns of change in the topological organization of individual level gray matter morphological networks in children with autism spectrum disorder(ASD).Materials and Methods The clinical and high-resolution T1 structural image data of 52 ASD children and 69 typical developing children from Autism Brain Imaging Data Exchange public database were selected.The individual gray matter structural networks were constructed by calculating the similarities of regional gray matter morphology.The global and local topological metrics of individual gray matter networks were analyzed using a graph theory method and compared between the two groups.Partial correlation analysis was used to evaluate the correlation between topological metrics showing significant between group differences and clinical characteristics in the ASD group.Results The verbal IQ was significantly lower in the ASD group compared with the typical developing group(t=-3.151,P=0.002),and no significant differences were found in full scale IQ and performance IQ between the two groups(P>0.05).Compared with the typical developing group,the ASD group showed significantly higher global efficiency(P=0.014),lower clustering coefficient(P=0.044)and characteristic path length(P=0.020)of gray matter structural networks.In the ASD group,abnormal nodal centralities were found in multiple brain regions(P<0.05,false discovery rate corrected),including the bilateral dorsolateral superior frontal gyrus,bilateral precuneus,bilateral caudate nucleus,right superior temporal gyrus,left middle temporal gyrus and bilateral anterior cingulate gyrus.No significant associations were found between the abnormal network topological metrics and IQ scores in the ASD group(r=0.069-0.187,all P>0.05).Conclusion Altered topological organization of individualized gray matter morphological networks is found in children with ASD,as characterized by decreased local segregation and increased global integration of networks.The areas with abnormal nodal centralities in children with ASD are mainly located at the brain regions involved in emotional processing,social and high-order cognitive functions.

The study was a prospective observational study. A total of 24 patients who underwent maintenance hemodialysis (MHD) at Haidian Hospital in Beijing from May 2024 to June 2024 were included as the study subjects. The safety and efficacy of a new single-needle dialysis in MHD patients were evaluated. The reasons for using single-needle dialysis included waiting for the maturity of internal fistula(7 cases, 29.17%), autogenous arteriovenous fistula thrombosis occurred (6 cases, 25.00%), puncture difficulty occurred (7 cases, 29.17%), and pain sensitivity or elderly (4 cases, 16.67%). The results showed that the average blood flow was (155.65±5.90) ml/min, total blood volume was (35.92±2.65) L during single-needle dialysis. One patient had slight puncture leakage, and the puncture success rate was 95.83%. Relevant indicators of dialysis adequacy showed that the average urea clearance (Kt/V) was 0.90±0.42, urea reduction ratio was 58.31%±7.93%, and online real-time Kt/V monitoring average value was 0.98±0.55. The results suggest that the application of the new improved single-needle dialysis mode in MHD patients is safe and effective.

Purpose To investigate the patterns of change in the topological organization of individual level gray matter morphological networks in children with autism spectrum disorder(ASD).Materials and Methods The clinical and high-resolution T1 structural image data of 52 ASD children and 69 typical developing children from Autism Brain Imaging Data Exchange public database were selected.The individual gray matter structural networks were constructed by calculating the similarities of regional gray matter morphology.The global and local topological metrics of individual gray matter networks were analyzed using a graph theory method and compared between the two groups.Partial correlation analysis was used to evaluate the correlation between topological metrics showing significant between group differences and clinical characteristics in the ASD group.Results The verbal IQ was significantly lower in the ASD group compared with the typical developing group(t=-3.151,P=0.002),and no significant differences were found in full scale IQ and performance IQ between the two groups(P>0.05).Compared with the typical developing group,the ASD group showed significantly higher global efficiency(P=0.014),lower clustering coefficient(P=0.044)and characteristic path length(P=0.020)of gray matter structural networks.In the ASD group,abnormal nodal centralities were found in multiple brain regions(P<0.05,false discovery rate corrected),including the bilateral dorsolateral superior frontal gyrus,bilateral precuneus,bilateral caudate nucleus,right superior temporal gyrus,left middle temporal gyrus and bilateral anterior cingulate gyrus.No significant associations were found between the abnormal network topological metrics and IQ scores in the ASD group(r=0.069-0.187,all P>0.05).Conclusion Altered topological organization of individualized gray matter morphological networks is found in children with ASD,as characterized by decreased local segregation and increased global integration of networks.The areas with abnormal nodal centralities in children with ASD are mainly located at the brain regions involved in emotional processing,social and high-order cognitive functions.

The study was a prospective observational study. A total of 24 patients who underwent maintenance hemodialysis (MHD) at Haidian Hospital in Beijing from May 2024 to June 2024 were included as the study subjects. The safety and efficacy of a new single-needle dialysis in MHD patients were evaluated. The reasons for using single-needle dialysis included waiting for the maturity of internal fistula(7 cases, 29.17%), autogenous arteriovenous fistula thrombosis occurred (6 cases, 25.00%), puncture difficulty occurred (7 cases, 29.17%), and pain sensitivity or elderly (4 cases, 16.67%). The results showed that the average blood flow was (155.65±5.90) ml/min, total blood volume was (35.92±2.65) L during single-needle dialysis. One patient had slight puncture leakage, and the puncture success rate was 95.83%. Relevant indicators of dialysis adequacy showed that the average urea clearance (Kt/V) was 0.90±0.42, urea reduction ratio was 58.31%±7.93%, and online real-time Kt/V monitoring average value was 0.98±0.55. The results suggest that the application of the new improved single-needle dialysis mode in MHD patients is safe and effective.

Objective To investigate the change in interleukin-33 (IL-33) in the peripheral blood of hepatocellular carcinoma (HCC) patients and the role and potential mechanism of IL-33 in regulating CD8 + T cell function in HCC patients. Methods A total of 44 HCC patients who attended Shaanxi Provincial People's Hospital from April 2019 to January 2020 and 20 healthy controls were enrolled. Peripheral blood was collected, and plasma and peripheral blood mononucleated cells (PBMCs) were isolated; ELISA was used to measure the plasma levels of IL-33 and its receptor ST2, and quantitative real-time PCR was used to measure the relative mRNA expression levels of IL-33 and ST2 in PBMCs. CD8 + T cells were purified and stimulated with recombinant IL-33; CCK-8 assay was used to assess cell proliferation, enzyme-linked immunospot assay was used to measure the secretion of perforin and granzyme B, and flow cytometry was used to measure the expression of PD-1, LAG-3, and CTLA-4; changes in cell proliferation, secretion of cytotoxic molecules, and immune checkpoint molecules after IL-33 stimulation were compared. CD8 + T cells were co-cultured with HepG2 cells; the expression of lactate dehydrogenase was measured to calculate the proportion of dead HepG2 cells induced by CD8 + T cells, and the change in the killing function of CD8 + T cells after IL-33 stimulation was compared. The t -test or the paired t -test was used for comparison of continuous data between two groups, and a Pearson correlation analysis was performed. Results Compared with the control group, the HCC group had significantly lower plasma level of IL-33 (269.80±63.08 pg/ml vs 339.50±64.43 pg/ml, t =4.072, P < 0.001) and relative mRNA expression level of IL-33 in PBMCs (1.07±0.14 vs 2.45±0.87, t =10.250, P < 0.001). There were no significant differences in the plasma level of ST2 and the relative mRNA expression level of ST2 in PBMCs between the HCC group and the control group ( P > 0.05). The proportion of CD8 + T cells was not correlated with the plasma level of IL-33 or ST2 (both P > 0.05). Compared with the control group, the HCC group had significantly lower levels of perforin and granzyme B (both P < 0.05) and a significantly higher proportion of CD8 + T cells with positive PD-1, LAG-3, and CTLA-4 ( P < 0.05). Stimulation with recombinant IL-33 did not affect the proliferation of CD8 + T cells or the expression of immune checkpoint molecules ( P > 0.05), but it promoted the secretion of perforin and granzyme B ( P < 0.05). Compared with the control group, the HCC group had a significant reduction in the killing activity of CD8 + T cells ( P < 0.05), and stimulation with recombinant IL-33 enhanced the killing function of CD8 + T cells, which was mainly reflected in the increases in the proportion of dead HepG2 cells ( P < 0.05) and the secretion of IFNγ and TNFα ( P < 0.05). Conclusion There is a reduction in the plasma level of IL-33 in HCC patients. IL-33 can enhance the killing activity of CD8 + T cells by promoting the secretion of perforin and granzyme B, which provides a new target for the treatment of HCC.

Objective:To investigate the clinical efficacy of sildenafil in patients with systemic lupus erythematosus complicated with pulmonary hypertension and its effect on serum levels of Type 17 helper T cells (Th17) and thrombospondin-1(TSP-1).Methods:A total of 70 patients with systemic lupus erythematosus complicated with pulmonary hypertension treated in Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine from January 2017 to July 2019 were randomly divided into observation group and control group, with 35 cases in each group by the envelope method. The control group was given conventional treatment, while the observation group was given sildenafil on the basis of the treatment of the control group. The clinical efficacy, systemic lupus erythematosus disease activity index (SLEDAI), and pulmonary artery blood pressure (PASP), and serum Th17 and TSP-1 levels in the two groups before and after treatment were compared.Results:The total effective rate of the observation group (94.29%) was much higher than that of the control group (71.43%), with statistically significant difference ( P<0.05). After treatment, the SLEDAI score and PASP of each group were lower than those before treatment, and the observation group had greater decreases in SLEDAI score and PASP, with statistically significant differences ( P<0.05). After treatment, the Th17 and TSP-1 levels of each group were lower than those before treatment, and the observation group had greater decreases in Th17 and TSP-1 levels, with statistically significant differences ( P<0.05). Conclusion:Sildenafil is effective in the treatment of systemic lupus erythematosus complicated with pulmonary hypertension by reducing the levels of serum Th17 and TSP-1.

Objective:To investigate the clinical efficacy of sildenafil in patients with systemic lupus erythematosus complicated with pulmonary hypertension and its effect on serum levels of Type 17 helper T cells (Th17) and thrombospondin-1(TSP-1).Methods:A total of 70 patients with systemic lupus erythematosus complicated with pulmonary hypertension treated in Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine from January 2017 to July 2019 were randomly divided into observation group and control group, with 35 cases in each group by the envelope method. The control group was given conventional treatment, while the observation group was given sildenafil on the basis of the treatment of the control group. The clinical efficacy, systemic lupus erythematosus disease activity index (SLEDAI), and pulmonary artery blood pressure (PASP), and serum Th17 and TSP-1 levels in the two groups before and after treatment were compared.Results:The total effective rate of the observation group (94.29%) was much higher than that of the control group (71.43%), with statistically significant difference ( P<0.05). After treatment, the SLEDAI score and PASP of each group were lower than those before treatment, and the observation group had greater decreases in SLEDAI score and PASP, with statistically significant differences ( P<0.05). After treatment, the Th17 and TSP-1 levels of each group were lower than those before treatment, and the observation group had greater decreases in Th17 and TSP-1 levels, with statistically significant differences ( P<0.05). Conclusion:Sildenafil is effective in the treatment of systemic lupus erythematosus complicated with pulmonary hypertension by reducing the levels of serum Th17 and TSP-1.

Objective:To investigate the safety and efficacy of sacral neuromodulation(SNM)therapy for the treatment of lower urinary tract dysfunction(LUTD)in elderly patients.Methods:Clinical data of 91 elderly patients with LUTD from multiple medical institutions who received SNM during the period from January 2012 to December 2016 were retrospectively analyzed.Patients were divided into four groups: the interstitial cystitis(IC)group(n=28), the neurogenic bladder(NB)group(n=36), the overactive bladder syndrome(OAB)group(n=13)and the idiopathic dysuria(ID)group(n=14). Different sets of evaluation parameters were used for different diseases.Patients’ baseline data and data in stage I(test phase)and stage Ⅱ(permanent SNM)were recorded, statistically analyzed and compared.Results:Ninety-one people underwent SNM treatment.Of them, 53 patients received permanent implants(stage Ⅱ), and the total conversion rate of stage I to stage Ⅱ was 58.2%(53/91). Patients receiving permanent implants(stage Ⅱ)had a preoperative period ranging from 3 months to 30 years, and were followed up for 2 to 58 months after treatment, with an average follow-up of 19.6 months.The improvement rates in stage I for urinary urgency, daily urination frequency, daily nocturnal urination frequency, maximum urine volume, daily average urine volume, daily urine leakage frequency, and quality of life score were 35.4%, 31.6%, 33.7%, 32.6%, 49.2%, 43.2% and 13.2%, respectively.The improvement rates in stage Ⅱ for urinary urgency, daily urination frequency, daily nocturnal urination frequency, maximum urine volume, daily average urine volume, daily urine leakage frequency, and quality of life score were 43.2%, 40.0%, 37.8%, 50.5%, 70.5%, 70.4% and 43.2%, respectively.Three adverse events occurred, including 1 case of recurrent symptoms, 1 case of moderate infection, and 1 case of electrical lead dislocation.Conclusions:Sacral nerve stimulation has definitive and consistent curative effects on LUTD in elderly people.The follow-up time should be extended to further study the safety of sacral nerve stimulation.

OBJECTIVE: explore the expression of miR-155-5p in Wilms tumor and its effect in regulating the proliferation, migration and apoptosis of Wilms tumor cells. METHODS: Specimens of tumor tissues and paired adjacent tissues were obtained from 40 patients with Wilms tumor for detection of the expression levels of miR-155-5p using RT-qPCR. Wilms tumor cell line G401 was transfected with miR-155-5p mimics and miR-155-5p inhibitor to induce miR-155-5p over-expression and its inhibition, respectively, and the changes in the cell proliferation, migration and apoptosis were assessed using cell counting kit-8 (CCK-8), wound healing assay and fl ow cytometry. RESULTS: RT-qPCR showed that the expression of miR-155-5p decreased significantly in Wilms tumor tissues as compared with normal kidney tissues and was significantly associated with TNM stage ( < 0.05). In G401 cells, over-expression of miR-155-5p significantly inhibited the cell proliferation and migration and promoted cell apoptosis ( < 0.05), and down-regulation of miR-155-5p obviously enhanced the proliferation and migration and suppressed apoptosis of the cells ( < 0.05). CONCLUSIONS miR-155-5p is down-regulated in Wilms tumor and its expression level is correlated with TNM stage. miR-155-5p participates in the progression of Wilms tumor by inhibiting the proliferation and migration and promoting apoptosis of the tumor cells, and may serve as a novel biomarker for diagnosis, therapy and prognostic evaluation of Wilms tumor.
Apoptosis ; Cell Movement ; Cell Proliferation ; Humans ; Kidney Neoplasms ; genetics ; MicroRNAs ; genetics ; Neoplasm Invasiveness ; Wilms Tumor ; genetics

Objective: To investigate the effect factors of liver enzymes elevation by monitoring the liver function changes before and after intraportal islet transplantation. Methods: 16 diabetic patients who received intraportal islet transplantation in our hospital were analyzed. The levels of aspartic aminotransferase (AST), alanine aminotransferase (ALT)and total bilirubin (TBil)were monitored after islet transplantation. Results: Among those 16 diabetic patients who received intraportal islet transplantation, 11 patients showed an increased AST and 8 patients showed an increased ALT, among which a 2.5-fold increase in AST was observed in 4 patients and over 1.5-fold elevation of ALT was observed in 3 patients. The level of TBil were in the normal range before and after transplantation in all patients. Transplanted tissue volume of islet was the main factor for significantly increased AST (P<0.05) in this study. It is also shown that the change in portal pressure is related to the AST elevation after islet transplantation. Conclusions The amount of transplanted islet tissue volume is related to the liver enzymes elevation after intraportal islet transplantation. Therefore, the improvement of the purity of islet to reduce the amount of transplanted tissue could be benefit to prevent the liver injury after islet transplantation. Meanwhile, the purified islets should be injected as slowly as possible to maintain a stable portal pressure.