Objective To observe the safety and effectiveness of acupuncture in young and middle-aged women suffering from insomnia with liver-depression using liver-soothing and mind-regulating method.Methods Totally 76 female patients with insomnia of liver-depression were randomly divided to the acupuncture group and the sham-acupuncture group,with 38 cases in each group.The treatment group received acupuncture with the method of liver-soothing and mind-regulating,while the sham-acupuncture group used the same acupoints with simulated stimulation using blunt-tip needles.Treatment lasted 8 weeks,with a total of 18 sessions,as well as a 3-month follow-up period.The Pittsburgh Sleep Quality Index(PSQI),Insomnia Severity Index(ISI),and Self-Rating Anxiety Scale(SAS)were measured before treatment,at the week 4 and week 8,and at 1-and 3-month follow-ups.The adverse reaction of both groups were monitored.Results Four cases in the acupuncture group and 2 cases in the sham-acupuncture group were lost.Compared with before treatment,the total score and factor scores of PSQI,ISI and SAS of the two groups at 4 and 8 weeks of treatment and the first and third months of follow-up significantly decreased(P<0.05);compared with the sham-acupuncture group at the same time point,the total score of PSQI,ISI and SAS in the acupuncture group decreased significantly at 8 weeks of treatment(P<0.05).No serious adverse events occurred in the two groups during the treatment.Conclusion The acupuncture of liver-soothing and mind-regulating method is a safe and effective treatment for improving sleep quality and anxious mood of young and middle-aged female patients with liver-depression type of insomnia.

Objective To observe the safety and effectiveness of acupuncture in young and middle-aged women suffering from insomnia with liver-depression using liver-soothing and mind-regulating method.Methods Totally 76 female patients with insomnia of liver-depression were randomly divided to the acupuncture group and the sham-acupuncture group,with 38 cases in each group.The treatment group received acupuncture with the method of liver-soothing and mind-regulating,while the sham-acupuncture group used the same acupoints with simulated stimulation using blunt-tip needles.Treatment lasted 8 weeks,with a total of 18 sessions,as well as a 3-month follow-up period.The Pittsburgh Sleep Quality Index(PSQI),Insomnia Severity Index(ISI),and Self-Rating Anxiety Scale(SAS)were measured before treatment,at the week 4 and week 8,and at 1-and 3-month follow-ups.The adverse reaction of both groups were monitored.Results Four cases in the acupuncture group and 2 cases in the sham-acupuncture group were lost.Compared with before treatment,the total score and factor scores of PSQI,ISI and SAS of the two groups at 4 and 8 weeks of treatment and the first and third months of follow-up significantly decreased(P<0.05);compared with the sham-acupuncture group at the same time point,the total score of PSQI,ISI and SAS in the acupuncture group decreased significantly at 8 weeks of treatment(P<0.05).No serious adverse events occurred in the two groups during the treatment.Conclusion The acupuncture of liver-soothing and mind-regulating method is a safe and effective treatment for improving sleep quality and anxious mood of young and middle-aged female patients with liver-depression type of insomnia.

Objective To evaluate perioperative nutritional support for patients with acute abdomen in enhanced recovery after surgery (ERAS) programme.Methods A total of 490 patients with acute abdominal disease were collected,287 in ERAS group and 203 in conservative perioperative management (CPM) group.Biochemical and clinical markers of the 2 groups were compared.Results ERAS group had higher plasma albumin level,quicker bowel function recovery,lower postoperative complications,shorter hospital stay,and a lower WHO pain rating scale (all P ＜ 0.05).Conclusion With perioperative nutritional support,ERAS programme can accelerate recovery after emergency surgery,reduce the rate of overall complications,promote bowel function recovery,and decrease morbidity in the perioperative period for patients with acute abdominal disease.

Objective To analyze the complications of transvaginal natural orifice transluminal endoscopic surgery( TV-NOTES) nephrectomy, and to explore effective measures to prevent and manage those complication.Methods From May 2010 to January 2015, a total of 178 females who had been married and given birth underwent TV-NOTES nephrectomy in our center.The average age was 47 ( ranging 23 to 71 ) years and the average BMI was 23.6 ( ranging 14.7 to 31.9 ) kg/m2.Pathological diagnosis included 142 cases of non-functional kidneys, 29 cases of renal tumors ( T1 N0-1 M0 25 cases, T2 N0-1 M0 4 cases) , and 7 cases of renal tuberculosis.One hundred and sixty hybrid TV-NOTES nephrectomy procedures (simple in 132, radical in 28) and 18 pure TV-NOTES nephrectomy procedures (simple in 17, radical in 1) were performed.Intraoperative and postoperative complications were graded according to Satava and Clavien-Dindo grade classifications.The major complications and relative treatments were analyzed.Results Among the 178 TV-NOTES nephrectomy procedures, there were 40 ( 22.5%) complications occurred, including 13 (7.3%) major complications, in which there were 11 cases of intraoperative complications (6.2%),2 cases of postoperative complications (1.1%).All the complications were successfully managed using organ repair or resection, embolectomy, hemostasis, and so on.No intraoperative and postoperative deaths occurred.There was no significant difference in major complications between hybrid TV-NOTES and pure TV-NOTES nephrectomy (6.9%vs.11.1%, P=0.620).The intraoperative major complications in the early developmental stage of TV-NOTES nephrectomy were more than that of the late stage (20.0%vs.3.4%, P=0.004).There was no significant difference in postoperative complications between the early and late developmental stage of TV-NOTES nephrectomy (13.3%vs.10.8%, P=0.751).Conclusions TV-NOTES nephrectomy is safe and feasible, but there are some major complications worthy of attention.Personal prevent and treatment strategy should be considered.

Objective To explore the predictive value of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) level in anthracycline-based chemotherapy-related cardiotoxicity of breast cancers.Methods A total of 135 breast cancer patients was analyzed if NT-proBNP was associated with chemotherapy-related cardiotoxicity.The level of NT-proBNP in the diagnosis of cardiotoxicity was assessed.Results A total of 22 patients (16.29％) had subsequent claims for cardiotoxicity events.NT-proBNP in cardiotoxicity group was significantly higher than that non-cardiotoxicity group (P ＜ 0.05).According to receiver operating characteristic (ROC) curve, the cut-off value of NT-proBNP was set at 350 pg/ml, specificity and sensitivity were 70.32％ and 82.58％ , respectively.Positive and negative predictive values were 78.12％ and 65.45％, respectively.Conclusions The present study is to confirm excellent clinical value of NTproBNP on cardiotoxicity.The level of NT-proBNP for early detection of cardiotoxicity has good prospects for high risk patients.

Objective To explore the clinical value of high-frequency color Doppler ultra-sonography in identifying axillary metastatic and benignant lymph nodes of breast cancer.Methods 106 axillary enlarged lymph nodes of 78 patient with breast cancer were retrospectively analyzed by high-frequency two-dimensional ultrasonography combined with color Doppler flow imaging (CD-FI).The sonograms and characteristics of blood flow were compared between 56 axillary metastatic enlarged lymph nodes and 50 axillary benignant enlarged lymph nodes.Results There were signifi-cant differences of L /S,thickness-increased characteristics of skin texture,presence or absence of confluent shape and calcified plaque,distribution characteristics of blood flow signals and degrees of blood flow between axillary metastatic and benignant enlarged lymph nodes of breast cancer.Conclu-sion Axillary metastatic enlarged lymph nodes of breast cancer are greatly different from axillary benignant enlarged lymph nodes in the shape,internal echo and blood flow patterns.High-frequen-cy color Doppler ultrasonography is a useful method with higher clinical application value for identi-fying axillary metastatic and benignant enlarged lymph nodes of breast cancer.

Objective To explore the clinical value of high-frequency color Doppler ultra-sonography in identifying axillary metastatic and benignant lymph nodes of breast cancer.Methods 106 axillary enlarged lymph nodes of 78 patient with breast cancer were retrospectively analyzed by high-frequency two-dimensional ultrasonography combined with color Doppler flow imaging (CD-FI).The sonograms and characteristics of blood flow were compared between 56 axillary metastatic enlarged lymph nodes and 50 axillary benignant enlarged lymph nodes.Results There were signifi-cant differences of L /S,thickness-increased characteristics of skin texture,presence or absence of confluent shape and calcified plaque,distribution characteristics of blood flow signals and degrees of blood flow between axillary metastatic and benignant enlarged lymph nodes of breast cancer.Conclu-sion Axillary metastatic enlarged lymph nodes of breast cancer are greatly different from axillary benignant enlarged lymph nodes in the shape,internal echo and blood flow patterns.High-frequen-cy color Doppler ultrasonography is a useful method with higher clinical application value for identi-fying axillary metastatic and benignant enlarged lymph nodes of breast cancer.

OBJECTIVE: To explore the relation between human papillomavirus (HPV16) infection and expression of Toll-like receptor 4 (TLR4) in cervical cell lines and cervical lesion tissues and to investigate the effect of TLR4 on cervical cancer progression. METHODS: Expression of HPV16 E6 mRNA was detected by RT-PCR. Western blot and immunohistochemistry were used to detect the expression of TLR4 in H8, SiHa, Caski cell lines and formalin-fixed and paraffin-embedded cervical tissue specimens with cervicitis, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinama (CSCC). DNA was extracted from paraffin-embedded cervical cancer tissues and HPV16 genes were detected. RESULTS: The differentiation expression of HPV16 E6 mRNA and TLR4 in SiHa and Caski was significantly higher than that of normal cervical cell H8 (P<0.05). The positive expression rates of TLR4 and HPV16 in chronic cervicitis, CIN, and cervical cancer were 32.0%, 59.4%, and 77.8% (P<0.01) and 8.0%, 48.4%, and 81.0% (P<0.01), respectively. Up-regulation of TLR4 was correlated with tumor differentiation (P<0.01), but not with FIGO stages or lymph node metastasis (P>0.05). The expression of TLR4 was significantly correlated with HPV16 infection in CIN and CSCC (r=0.303, P<0.05, r=0.633, P<0.05). CONCLUSION High expression of TLR4 may play important roles in the development and progression of CIN and CSCC, and the expression of TLR4 can be up-regulated by HPV16 infection.
Carcinoma, Squamous Cell ; metabolism ; virology ; Cell Line, Tumor ; Cervical Intraepithelial Neoplasia ; metabolism ; virology ; Female ; Human papillomavirus 16 ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Oncogene Proteins, Viral ; metabolism ; Papillomavirus Infections ; metabolism ; RNA, Messenger ; Repressor Proteins ; metabolism ; Toll-Like Receptor 4 ; metabolism ; Up-Regulation ; Uterine Cervical Neoplasms ; metabolism ; virology

Objective:To obtain the full length cDNA sequences of hepatoma associated gene HTA, analyze its alternative splicing, detect the expression pattern of 2 HTA gene transcripts in different hepatic cell lines, and to establish a base for further study of HTA gene function in hepatocellular carcinoma (HCC) occurence and development. Methods:The full length cDNA of HTA gene was cloned by rapid amplification of cDNA 3' ends (3'-RACE), rapid ampliifcation of cDNA 5' ends (5'-RACE) and DNA sequencing. The gene structure and alternative splicing were analysed. Northern blot assay was performed to detect the expression pattern of 2 HTA gene transcripts in different hepatic cell lines. Results:The full length of HTA gene was 1414 bp, composed of 3 exons and 2 introns, and the second intron could be retained in mRNA. Northern blot assay showed that 2 transcripts of HTA mRNA(1.4 kb and 1.7 kb) could express in the HCC cell lines HepG2 and QGY-7703, but not in the non-malignant cell line L-02 and HUVEC. The expression level of 1.4 kb transcript was much higher than 1.7 kb one. Conclusion:This study successfully has obtained the full length cDNA of HTA gene, and analysed the gene sequence and alternative splicing, 2 transcripts of HTA mRNA specifically expressed in HCC cell lines. As a hepatoma associated gene, HTA deserves further investigation.

OBJECTIVE: To recombinant express hepatoma associated gene(HTA) and pre-test the function of HTA to determine the role of HTA in the development of liver cancer. METHODS: HTA338-616 was amplified from HepG2 cells and cloned into the prokaryotic expression vector pET21a(+)-MBP. The proteins MBP and MBP-HTA were induced, purified by His-tag magnetic bead purification kit and identified by Western blot and ELISA. HepG2 cells were stimulated with MBP or MBP-HTA proteins. MTT assay and colony formation assay were employed to examine the proliferation of these cells and the changes of cell cycle distribution were determined by flow cytometry. RESULTS: The prokaryotic expression plasmid pET21a(+)-MBP-HTA was successfully constructed. We got a 52 kD purified purpose protein.The proliferation of HepG2 cells stimulated with MBP-HTA was significantly higher than those stimulated with MBP and negative controls. HepG2 cells stimulated with MBP-HTA showed significant decrease fraction in G1 phase and increase fraction in S phase, and the cell proliferation was enhanced. CONCLUSION HTA protein can significantly promote the proliferation of HepG2 cells, which may be related to the promotion of G1 phase to S phase.
Base Sequence ; Cell Proliferation ; drug effects ; Escherichia coli ; genetics ; metabolism ; Genes, Neoplasm ; physiology ; Genetic Vectors ; Hep G2 Cells ; Humans ; Maltose-Binding Proteins ; biosynthesis ; genetics ; pharmacology ; Molecular Sequence Data ; Neoplasm Proteins ; biosynthesis ; genetics ; pharmacology ; Open Reading Frames ; genetics ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; pharmacology