Objective To analyze the distribution characteristics of pathogenic bacteria in ear canal secretions and the results of drug sensitivity in children with acute suppurative otitis media,and to provide a basis for rational drug use in early stage.Methods From January 2020 to January 2025,a total of 95 children with acute suppurative otitis media in Taian Maternity and Child Health Hospital were selected to analyze the distribution characteristics and drug resistance of pathogenic bacteria in ear canal secretions.Results A total of 82 strains of pathogenic bacteria were isolated.The 77 strains were Gram-positive bacteria,and 5 strains were Gram-negative bacteria.The most common pathogenic bacteria were Streptococcus pneumoniae(40.3％),Staphylococcus aureus(32.9％)and Streptococcus pyogenes(13.4％).Among 27 strains of Staphylococcus aureus,7 strains were methicillin resistant Staphylococcus aureus(MRSA).The detection rate of pathogenic bacteria in ear canal secretions varied in four seasons,with the highest in autumn and the lowest in spring,and the difference was not statistically significant(x2=3.142,P=0.370).The proportion of pathogenic bacteria in ear canal secretion was different in four seasons.Streptococcus pneumoniae was the most detected in spring,autumn and winter,and Staphylococcus aureus was the most detected in summer.The resistance rates of Streptococcus pneumoniae to erythromycin,clindamycin and compound sulfamethoxazole(＞75.0％)were higher.The resistance rates of Streptococcus pyogenes to erythromycin,clindamycin and tetracycline were 100％.The resistance rates of methicillin sensitive Staphylococcus aureus to penicillin,erythromycin and clindamycin(≥ 75.0％)were higher.The resistance rates of MRSA to penicillin was 100％.The resistance rates of Staphylococcus aureus to vancomycin,linezolid,tigecycline and rifampicin were not detected.Conclusion The common pathogenic bacteria in ear canal secretions from children with acute suppurative otitis media are Streptococcus pneumoniae,Staphylococcus aureus and Streptococcus pyogenes,and the distribution of pathogenic bacteria is different among different seasons.Antibiotics commonly used in clinic have high drug resistance,and the isolation and culture of pathogenic bacteria and drug sensitivity analysis should be carried out as early as possible,so as to help improve the treatment effect and the prognosis.

Objective To analyze the distribution characteristics of pathogenic bacteria in ear canal secretions and the results of drug sensitivity in children with acute suppurative otitis media,and to provide a basis for rational drug use in early stage.Methods From January 2020 to January 2025,a total of 95 children with acute suppurative otitis media in Taian Maternity and Child Health Hospital were selected to analyze the distribution characteristics and drug resistance of pathogenic bacteria in ear canal secretions.Results A total of 82 strains of pathogenic bacteria were isolated.The 77 strains were Gram-positive bacteria,and 5 strains were Gram-negative bacteria.The most common pathogenic bacteria were Streptococcus pneumoniae(40.3％),Staphylococcus aureus(32.9％)and Streptococcus pyogenes(13.4％).Among 27 strains of Staphylococcus aureus,7 strains were methicillin resistant Staphylococcus aureus(MRSA).The detection rate of pathogenic bacteria in ear canal secretions varied in four seasons,with the highest in autumn and the lowest in spring,and the difference was not statistically significant(x2=3.142,P=0.370).The proportion of pathogenic bacteria in ear canal secretion was different in four seasons.Streptococcus pneumoniae was the most detected in spring,autumn and winter,and Staphylococcus aureus was the most detected in summer.The resistance rates of Streptococcus pneumoniae to erythromycin,clindamycin and compound sulfamethoxazole(＞75.0％)were higher.The resistance rates of Streptococcus pyogenes to erythromycin,clindamycin and tetracycline were 100％.The resistance rates of methicillin sensitive Staphylococcus aureus to penicillin,erythromycin and clindamycin(≥ 75.0％)were higher.The resistance rates of MRSA to penicillin was 100％.The resistance rates of Staphylococcus aureus to vancomycin,linezolid,tigecycline and rifampicin were not detected.Conclusion The common pathogenic bacteria in ear canal secretions from children with acute suppurative otitis media are Streptococcus pneumoniae,Staphylococcus aureus and Streptococcus pyogenes,and the distribution of pathogenic bacteria is different among different seasons.Antibiotics commonly used in clinic have high drug resistance,and the isolation and culture of pathogenic bacteria and drug sensitivity analysis should be carried out as early as possible,so as to help improve the treatment effect and the prognosis.

Objective To study the effect of clove oil on bacterial biofilm of Streptococcus pneumoniae. Methods The components of clove oil were determined by GC-MS mass spectrometry. The suspension of streptococcus mutans was prepared to carry out the germicidal test, and to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of clove oil. The effects of different concentrations of clove oil on the formation of biofilm were observed by laser confocal microscopy, and the average fluorescence intensity of live bacteria and dead bacteria in biofilm was recorded. Results Determination of clove oil 8 components were analyzed by GC-MS mass spectrometry method, most of the Eugenol content was 48.93%, followed by the Caryophyllene 20.78%, Methylis salicylas 14.96%. Through MIC, MBC and the experimental results showed that clove oil had antibacterial activity of Proteus, and with the increase of clove oil concentration, the inhibitory effect was better. When it reached a certain concentration, it can showed bactericidal effect that the MIC and MBC were 0.3125%, 0.6250%. The clove oil also had inhibitory effect on Streptococcus mutans biofilm with the concentration depedence. When the concentration of clove oil was 2.5000%, the average fluorescence intensity (live/dead bacteria) was 0.082 ± 0.007, the proportion of living bacteria decreased obviously, and biofilm disappeared. Conclusions The clove oil not only has inhibitory effect on Streptococcus mutants, but also can inhibit and clear the biofilm formation of Streptococcus.