Introduction: The National Library of Mongolia was established in 1921 based largely on the 8th Khalkha Jebtsundamba Library. In studying the tradition of collecting and preserving books in Mongolia, the subject can be divided into three categories:
1. Family collection
2. Palace/ Royal collections
3. Monastery collections.
From the collection mentioned above, we selected a work belonging to the royal collections, specifically one transferred from the VIII Khalkha Jebtsundamba’s Library to the National Library. This book, “The drug actions that are useful for any kind of ulcer, swelling, and injuries,” is a rare and valuable item of medical cultural heritage, existing in a single known copy. Therefore, this work was selected for incorporation into the research cycle.
To identify the formulas, forms of medicines, methods of use, actions, and to abstain from diet methods in the book “The drug actions that are useful for any kind of ulcer, swelling, and injuries.” Methods: We employed methods such as the study of original texts and sources, analytic- synthetic method. Conclusion The book includes methods for using 27 various medicines to treat 26 types of diseases related to the lesions. Some medicines can be used externally in addition to oral administration, and 90% of all medicines are in the form of powders and granules for external application.

Introduction: The text “Secret Medicine Recipes of Important Matters” represents a unique heritage that embodies the theories and concepts of traditional Mongolian medicine, particularly as a documentary source of medicinal composition. Through this work, it is important to examine the terminology, theoretical and methodological concepts, and characteristics of medical works written in Mongolian script within the field of traditional Mongolian medicine.
To conduct a study of the final words of the second volume of the “Secret Medicine Recipes for Important Matters” and to compare and analyze the original text, including some outdated terms and content. Methods: The second volume of the book “Secret Medicine Recipes for Important Matters” is a Mongolian manuscript. The book measures 22.7x22.3 cm and contains 111 pages. It is covered with yellow silk featuring a floral pattern and is bound with four holes using twisted brownish-yellow thread. The text is written on coarse paper with red lines. Methods of textual analysis for ancient books and translated methods were used in the study. Conclusion The second volume of the “Secret Medicine Recipes for Important Matters” contains a description of the nasal immunization method of the white ash medicine used for disease prevention. The method involves administering the substance through the nose to regulate sputum, and from a modern perspective, it can be interpreted as a live vaccination. The most optimal version of this method was created by Tsakhar Geshe Luvsanchultem and written in his work, and later used as an injection for the three Gobi Gurvan Saikhan’s Sharav maaramba.

Introduction: A study sample of 78 manuscripts was selected from the 1,223 transferred from the 8^th Khalkha Jebtsundamba’s Library to the National Library of Mongolia. This sample consists of single, representative volumes from unique, multi-volume texts. Of the 78 manuscripts analyzed by Tungalag Batmunkh, eight (approx. 10%) are categorized as medical treatises. However, a systematic study conducted by Academician Bold Sharav identified a total of nine distinct volumes, thereby establishing a precise corpus of the works. One of these is the book “The book summarizes a number of conventional formulas from Tong Ren Tang Pharmaceutical Company”, which is one of the sources that has not yet been researched. This study was undertaken to determine the origin of the book, terminology, number, and category of medicines, and to clarify the drug action of some formulas.
To determine the origin, terminology, number, and classification of the ancient medical book “The book summarizes a number of conventional formulas from Tong Ren Tang Pharmaceutical Company,” and to clarify the characteristics of the medicines included in the chapter “Drugs for stroke diseases.” Methods: We employed methods such as the study of original texts and sources, and the checklist method. Conclusion The medications used in this book for stroke have the following benefits: thrombolytic, bone strengthening, tendon pain relief, support free breathing, relieve fever, antiplatelet, cure wind, regeneration bone marrow, improve testicle semen, and provide physical radiance, strength, and virility.

Background: Copper is a mineral that is involved in many functions og the human body and copper has antibacterial reaction. However many scientists have studied the antibacterial activity of nano copper, in our country, the lack of research is the basis of our research. Aim: Determination of antibacterial activity of colloidal copper (CuNP) Materials and Methods: The study protocol was reviewed and approved by the Research Ethics Review Committee of the Mongolian National University of Medical Sciences on February 18, 2022 (Protocol No. 2022/3-02). An ionized copper solution was produced by passing an electric current (19–20 V, 3–5 A, for 24 h) through sterilized distilled water using copper plates. Ascorbic acid was then added to prepare colloidal copper. The concentration was determined using a PPM meter, and the solution was filtered through a 0.22 µm bacterial filter. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against standard bacterial strains were determined by the broth dilution method. To determine the bactericidal time, bacterial cultures were exposed to the bactericidal concentration and subcultured hourly for 10 hours. Additionally, antibacterial activity was evaluated on Mueller–Hinton agar using the disk diffusion method according to CLSI standards, where 20 µL of colloidal copper was applied onto antibiotic disks. The plates were incubated at 37°C for 24 hours, and the diameter of the inhibition zones was measured. Result: The concentration of the prepared colloidal copper was 0.7 ppm. Using the broth dilution method, complete bactericidal activity was observed at 0.006 ppm against Klebsiella pneumoniae, Escherichia coli, and Salmonella enteritidis, while Pseudomonas aeruginosa was completely killed at 0.008 ppm. In terms of exposure time, complete bacterial killing occurred after 2 hours for S. enteritidis and K. pneumoniae, after 6 hours for E. coli, and after 11 hours for P. aeruginosa. In addition, antibacterial activity was evaluated on Mueller–Hinton agar using the disk diffusion method according to CLSI standards. Colloidal copper increased the inhibition zone diameter of most antibiotics by 1–5 mm, particularly against P. aeruginosa. In K. pneumoniae, the activity of aztreonam and ceftriaxone was enhanced. When evaluating the inhibition zone diameter of cefotaxime against E. coli, the susceptibility result changed from intermediate to susceptible, while no significant change was observed for the other antibiotics tested. For K. pneumoniae, the addition of colloidal copper increased the inhibition zone diameter of aztreonam and ceftriaxone by approximately 1–2 mm. In P. aeruginosa, the inhibition zone diameters of most antibiotics increased by 1–5 mm; however, the activity of imipenem decreased. For S. enteritidis, most antibiotics showed an increase in susceptibility of approximately 1–2 mm, while ceftriaxone showed no observable effect. Conclusion 1. Colloidal copper solution, at low concentrations, exhibits bactericidal activity against some Gram-negative rod-shaped bacteria. 2. Colloidal copper solution shows a synergistic effect with certain drugs, enhancing their activity; however, it exhibits an inhibitory effect on the activity of imipenem.

Background: Numerous international studies have established that the antibacterial activity of honey is attributed to its key components, including hydrogen peroxide, methylglyoxal, peptides, polyphenolic compounds, high sugar content, and acidic pH. Despite the vast consumption of different types of honey in Mongolia, there is a lack of sufficient scientific evidence on their antibacterial properties, which served as the rationale for conducting this study. Aim: A study comparing the antibacterial activity of honey commonly traded in Mongolia. Materials and Methods: The study was conducted using an experimental research design. Eight standard pathogenic bacterial strains were utilized: Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 1706, Staphylococcus aureus ATCC 25923, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 700699, Staphylococcus epidermidis ATCC 35984, Salmonella enterica ATCC 13076, and Acinetobacter baumannii ATCC 19606. A total of 14 honey samples were selected for the study, comprising 11 samples sourced from the Uvs, Tuv, Sukhbaatar, Khentii, Dornod, Darkhan, Selenge, and Khuvsgul provinces of Mongolia, and 3 Manuka-type honey samples from Australia. The samples were serially diluted in broth using a 96-well U-bottom microtiter plate. One microliter of each bacterial suspension was added, and the plates were incubated at 37°C for 18–24 hours. Subsequently, the cultures were spread onto Mueller-Hinton agar and incubated under the same conditions. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by evaluating bacterial growth inhibition and bactericidal activity, respectively. Result: Among the tested samples, the Manuka honey with MGO 570 and the honey collected from Tumentsogt soum in Sukhbaatar province exhibited the most potent antibacterial activity against MRSA, with a minimum inhibitory concentration (MIC) of 12.5% (v/v) and a minimum bactericidal concentration (MBC) of 25% (v/v). In contrast, the antibacterial activity of Manuka honey with UMF 5 and the honeys sourced from Tuv, Uvs, and Khalkhgol soum in Dornod province demonstrated MIC and MBC values of 25% (v/v) and 50% (v/v), respectively. The Manuka honey with MGO 30, as well as the honeys collected from Selenge, Darkhan, Sukhbaatar, Khentii, and Rashaant and Tarialan soums in Khuvsgul province, showed comparatively weaker activity against MRSA, Salmonella enterica, Staphylococcus epidermidis, Escherichia coli, and Staphylococcus aureus. Conclusion 1. The antibacterial activity of natural and cultivated floral honeys was assessed by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against eight standard bacterial strains. 2. All seven natural floral honey samples exhibited measurable antibacterial effects against the tested strains, whereas some of the cultivated floral honey samples showed no observable activity against certain bacterial strains.

Background: Helicobacter pylori is a highly prevalent pathogen infecting more than half of the global population, with considerable geographic variation in its genetic characteristics and antibiotic resistance patterns. The EPIYA motifs of the CagA protein play a critical role in bacterial virulence; however, data on the association between CagA genotypes and antibiotic resistance remain limited in Mongolia. Aim: To determine the antibiotic resistance, CagA EPIYA patterns, and clarithromycin resistance-associated mutations of H. pylori strains in Mongolia, and to evaluate the agreement between phenotypic and molecular diagnostic methods. Materials and Methods: Gastric biopsy specimens were collected from 210 patients undergoing endoscopy and cultured for H. pylori. Antibiotic susceptibility to metronidazole, clarithromycin, amoxicillin, levofloxacin, and tetracycline was determined using the E-Test method according to EUCAST guidelines. CagA EPIYA patterns were analyzed, and clarithromycin resistance-associated mutations (A2142C, A2142G, A2143G) were detected using real-time PCR. Result: H. pylori was successfully cultured in 86 of 210 samples (41%), and 61 isolates were included in the final analysis. CagA-positive strains accounted for 86.9%, with a predominance of Western-type CagA (52/53), while only one East Asian-type strain was identified. Among Western-type strains, the ABC (58.5%) and ABCC (37.7%) patterns were most common. The highest resistance rate was observed for metronidazole (70.5%), followed by levofloxacin (50.9%). Clarithromycin and amoxicillin resistance were both 19.7%, whereas no resistance to tetracycline was detected. Multidrug resistance (≥2 antibiotics) was observed in 45.9% of isolates, and 14.8% showed resistance to ≥3 antibiotics. Clarithromycin resistance-associated mutations were detected in 21.3% of isolates, predominantly A2143G. The agreement between E-test and real-time PCR methods was excellent (κ=0.95, p<0.001). Conclusion H. pylori strains in Mongolia are characterized by a predominance of Western-type CagA, with ABC and ABCC as the most common subtypes. Notably, the AAABC and AAAAB subtypes were identified for the first time. High levels of antibiotic resistance, particularly to metronidazole and levofloxacin, as well as a substantial prevalence of multidrug resistance, highlight the need for region-specific treatment strategies. In Mongolia, H. pylori demonstrated the highest resistance to metronidazole, accompanied by a high proportion of multidrug-resistant isolates.

The aim of this study was to assess the effectiveness of safety regulations governing traditional Mongolian horse racing on the frequency and severity of injuries among child jockeys. Regulations introduced in 2019 mandate the wearing of helmets and protective clothing, prohibit the participation of jockeys aged <7 years, and ban horse racing during the cold season (November–April). National injury surveillance data were used to compare the profile of injuries that occurred among children aged <15 years in the 4-year periods before and after the introduction of the regulations (2015–2018 and 2019–2022) and to investigate whether injuries continued to occur among underage children and during the banned season. The proportion of head injuries among injured children was calculated before and after the regulations were introduced. During the study periods, 6309 animal-riding injuries were recorded among children aged 3–14 years; 2539 occurred before the regulations were introduced and 3770 occurred after. Following the introduction of the regulations, the proportion of injured children aged <7 years decreased slightly. However, during 2019–2022, 294 animal-riding injuries were observed among underage children and 855 during the banned season. The proportion of head injuries among children with animal-riding injuries remained unchanged before and after the regulations were implemented (33.7% and 34.6%, respectively). The regulations have been ineffective. To reduce the burden of injuries among child jockeys, safety regulations need to be enforced throughout the year, and more stringent penalties for noncompliance should be imposed.

Background: Down syndrome (DS) is a chromosomal disorder and a leading cause of intellectual and physical developmental delay in children. In Mongolia, there is no current national guideline recommending the use of the quadruple biomarker test for prenatal screening of DS. Instead, triple marker testing during the second trimester remains standard practice. However, the quadruple test offers higher specificity and sensitivity. Therefore, this study was conducted to determine gestational age specific normative values for the quadruple markers, adjusted for ethnic characteristics. Currently, multiple of the median (MoM) values for Mongolian women are calculated using reference medians derived from Caucasian populations. This study aims to establish population-specific reference values for Mongolian women. Objective: To determine the normative serum levels of second-trimester quadruple biomarkers — alpha-fetoprotein (AFP), free beta-subunit of human chorionic gonadotropin (β-hCG), unconjugated estriol (uE3), and inhibin A (Inh-A) — in Mongolian pregnant women. Methods: A retrospective study was conducted involving pregnant women between 14 and 21 weeks of gestation. The concentrations of each biomarker were analyzed using multivariable quantile regression models, adjusting for gestational age and maternal weight. Results: MoM values calculated using the Mongolian model differed significantly from those based on the Caucasian model (Wilcoxon signed-rank test; p < 0.001). Specifically, the concentrations and MoM values for AFP and uE3 were significantly lower, while those for β-hCG and Inh-A were notably higher in the Mongolian cohort. Conclusion The serum levels of β-hCG and Inh-A were significantly elevated in Mongolian pregnant women compared to the ethnicity-adjusted Caucasian reference model, highlighting the importance of using population-specific normative data for accurate prenatal screening.

Introduction: Following the COVID-19 lockdown, the introduction of powered mopeds and scooters into urban mobility in Mongolia, without basic traffic regulatory frameworks such as vehicle classification, participation rules, age limits for riders, or parking regulations may have contributed to an increased risk of pedestrian injuries. Objective: To examine temporal trends in pedestrian injuries in Mongolia and to assess the impact of the emergence of micro mobility, while accounting for COVID-19 lockdowns, using an interrupted time-series analysis. Methods: We conducted an interrupted time-series analysis using national data on pedestrian injuries in Mongolia from January 2016 to December 2024. Monthly pedestrian injury rates per 10,000 population were calculated and stratified by age groups, regressing it on the number of months after January 2016 (the beginning of the study), after November 2020 (the lockdowns started), after May 2021 (the lockdown lifted and new mode of urban transportation started to release). Results: During the 9-year study period, there were 41,063 pedestrians, of whom 51.5% were male. The monthly rate of overall pedestrian injury started to decrease after the COVID-19 lockdowns possibly due to reduced traffic activity. However, following the lifting of restrictions, the trend started to increase among the age groups under 10 years (1.8%, 95% CI: 0.8–2.8), 10–19 years (1.6%, 95% CI: 0.3–2.9), 50–59 years (0.6%, 95% CI: 0.1–1.2), and 60–69 years (1.3%, 95% CI: 0.4–2.2). Conclusion The increase in pedestrian injuries suggests that the emergence of micromobility may have contributed to higher injury risk for pedestrians in Mongolia. This highlights the urgent need for change in infrastructure and regulations governing micromobility usage to enhance pedestrian safety.

Introduction: This time, we will clarify the terms used to express the traditional medical knowledge of Turks, Arabs, Persians, Khotons, or Uyghurs that have not been deeply explored by researchers in the book “Fundamental Principles of Food and Drink.” In addition, we propose to lay the foundation for studying the level of ancient medical relations between the ancient Mongols and Islamic countries and to create a new direction of research. Therefore, it is important to conduct a detailed study based on the sample of words related to food, drink, and herbal medicine of Islamic countries such as Turks, Arabs, Persians, and Uyghurs in the book “Fundamental Principles of Food and Drink” besides from Chinese medicine. The aim of the research: To explain the names of food and drink of Turkic, Arabic, and Khoton (Uyghur) origin in the “Fundamental Principles of Food and Drink” of 1330 AD. Research results Paul D. Buell and Eugene N. Anderson (2010), who translated the book “Fundamental Principles of Food and Drink” into English titled “A Soup for the Qan” with an explanation, have extensively and seriously considered how the book relates to the Islamic world. These researchers also say that “the diet, medicinal plants, and grains of the Islamic world reflect a common Middle Eastern (Iranian-Arab) culture rather than the specifics of the Turkic peoples. However, since Turkic elements are dominant in other parts of Islamic culture, there is no doubt that the Turkic forms of some Arabic and Iranian words are often found in “Fundamental Principles of Food and Drink.” Therefore, we have selected some words from Paul D. Buell and Eugene N. Anderson’s book “A Soup for the Qan” and considered them to be Turkic, Persian, and Arabic words. In fact, there are 36 Turkic origin words, 4 Persian origin words, and 5 Arabic origin words. However, if we look closely, we find that there are quite a few Mongolized Turkic words and Turkized Mongolian words. Conclusion: In the book “A Soup for the Qan” translated from Chinese with commentary into English by Paul D. Buell and Eugene N. Anderson, words of Turkic, Persian, and Arabic origin, namely 36 Turkic words, 4 Persian words, and 5 Arabic words, were selected and noted. Our research revealed that 11 of these 45 chosen words were Mongolian or Mongolian words of Turkic origin after thorough analysis. This result is in line with Bold Sharav’s 2022 book, which explained and noted that there were 23 Mongolian words in “Fundamental Principles of Food and Drink,” and it became clear that there were a total of 34 Mongolian or Mongolian words of Turkic origin words.