Malignant pleural effusion(MPE)is a common complication in patients with advanced malignant tumors,which not only significantly reduces their quality of life but also shortens their survival duration.Despite the widespread use of traditional treatment methods such as thoracentesis and pleurodesis,their efficacy is limited accompanied by high recurrence rates.Therefore,exploring novel therapeutic strategies becomes particularly urgent.In recent years,immunotherapy has attracted extensive attention for its potential in cancer treatment.This article systematically reviews the roles of CD4+T cell subsets,including regulatory T cells(Treg),T helper cell(Th)17,Th9,and Th22 cells,within the immunosuppressive microenvironment of MPE.These cell subsets are involved in the formation of the immunosuppressive state of MPE through various mechanisms and play key roles in the occurrence and development of the disease.In addition,the article discusses in detail the role of immune checkpoint molecules,such as programmed death protein 1(PD-1),PD-1 ligand(PD-L1),and cytotoxic T-lymphocyte-associated protein 4(CTLA-4),in the immune evasion of MPE.The abnormal expressions of these molecules provide opportunity for tumor cells to evade immune system surveillance.At the same time,this article also summarizes the application prospects of novel immunotherapy strategies,such as adoptive cell therapy(ACT)and chimeric antigen receptor T cell(CAR-T)therapy,in the treatment of MPE.These innovative therapies offer possibilities for improving the prognosis of MPE patients through activating and enhancing the anti-tumor immune response.

Abstract Digitalization provides adolescents with a variety of ways to entertain and socialize, but the new media environment may pose certain risks to their mental health. The paper expounds the relationship between social media exposure and social appearance anxiety, as well as the mediating role of social comparison. It also would enhance media health literacy as a potential solution. The aim is to clarify the formation mechanism of social appearance anxiety among adolescents in the digital media environment and provide governance recommendations to reduce social appearance anxiety among adolescents, so as to better help adolescents to resist risks and grow up healthy in the digital age.

Objective: To identify the 24-hour time allocation patterns of Mongolian junior school students, and to clarify the nature and degree of the influence of the time allocation patterns on the physical fitness of junior school students, so as to provide reference for optimizing and improving the scientific allocation of activity time of young students. Methods: In October 2022, 1 389 Mongolian junior school students aged 13-15 from four schools in Xing an League of Inner Mongolia Autonomous Region were selected with stratified cluster sampling method. The 24-hour time utilization of students were collected by using the questionnaire of "China Time Utilization Survey (CTUS) Time Log in 2017" from October 21 to 22, 2022, and physical fitness was evaluated by using the test data of the National Student Physical Health Standard from September to November 2022. Students time allocation patterns were determined by Latent Profile Analysis with Mplus software, and the correlation between the time allocation patterns and the physical fitness was analyzed by multiple linear regression. Results: The time allocation patterns of Mongolian junior school students could be classified into four types:learning type, screen type, diversity type, and sports type, and the population distribution were 24.7%, 18.3%, 9.9% and 47.1%, respectively. Among all gender and age groups, the sports type received highest total score physical fitness, followed by diversified type, learning type, and screen type ( F =72.25-154.94, P <0.01). The results of multiple regression analysis showed that gender, age, and time allocation patterns were significantly related to the total score of physical fitness ( P < 0.05 ). The total score of physical fitness in learning type, diversity type and sports type groups were all higher than that of screen type ( β =5.63, 12.86, 18.03, P <0.05). Moreover, the sports type showed highest effects on the total score of physical fitness, followed by the diversity type and learning type ( B =0.72, 0.31, 0.19, P <0.05). Conclusions There is a significant correlation between time allocation patterns and physical fitness of Mongolian junior school students, and those in sports type and diversity type of time allocation achieve better physical fitness, while those in learning type and screen type of time allocation shows relatively poorer physical fitness.In order to promote students physical fitness and healthy development, it is suggested to scientifically arrange students time allocation, increase physical activity time, and reduce sedentary activities that are detrimental to health.

Objective To propose a new multi-joint series venipuncture system, explore the mechanics and kinematics-based related control problems involved in needle insertion and needle picking during the puncture process, and verify feasibility of this system. Methods A puncture manipulator was built, and needle displacement control algorithm was proposed by combing with the puncture mechanics model. The the forward kinematics was calculated by using DH method, so as to obtain the tip coordinates. Then the inverse kinematics was calculated by using the geometric method. The forward and inverse processes were closely connected. The position error of the end coordinates before and after needle picking was compared by using the method of kinematics positive solution-inverse solution-re-positive solution. Finally, experimental verification and simulation were conducted by combining with the physical object. Results Through simulation and experiments, accuracy of the theoretical model was verified. The needle insertion algorithm could be used to achieve success with only one needle insertion, which provided theoretical basis for the control of robot arm. The position error before and after needle picking could be controlled within 1 mm from the end trajectory. The end needle tip of robot arm was almost kept fixed during the needle picking process. Therefore, this needle picking scheme was feasible and could basically verify that the needle picking action of robot arm met the accuracy and safety requirements. Conclusions The venipuncture manipulator truly simulates the needle insertion and needle picking action during the puncture process, and can safely and accurately realize the needle insertion and needle picking action with needle tip as the fixed point, indicating that it has certain clinical value.

Objective: To analysis the relationship between obesity types and exercise capacity among Mongolian primary school students, to provide evidence supporting the formulation of strategies to promote students physical fitness. Methods: A total of 7 941 Mongolian students aged 7-12 years in the Inner Mongolia Autonomous Region were selected, and data were collected from a field survey conducted by the National Physical Fitness and Health Survey in 2019. According to overweight and obesity screening of school aged children and adolescents, and the waist height ratio, obesity status was classified into general obesity, central obesity and complex obesity, relationships among obesity type, exercise ability and PFI. Results: The prevalence of obesity was 23.5%, 28.2%, 16.3% and 16.5% in boys and girls 7-9 and 10-12 years of age, respectively. The prevalence of obesity was higher in boys than girls( P <0.05). The detection rate of compound obesity in boys and girls was highest (15.6%, 9.2%; 18.4%, 8.2%), and the detection rate of compound obesity and central obesity in boys was higher than that in girls( P <0.05). The performance in the non obese boys and girls (on the basis of standing long jump, sit up and 50 m×8 round trip running in boys, and 50 m running, standing long jump, sit up and 50 m×8 round trip running in girls) was better than that in the obese groups( P <0.05). There were statistically significant differences in the median PFI of non obesity, general obesity, central obesity and compound obesity between boys and girls in the two groups of 7-9 and 10-12 years old (boys: H =79.23, 241.42; girls: H =61.94, 74.38, P <0.01). Conclusion The proportion of compound obesity among Inner Mongolian primary school students was relatively high, and the exercise capacity was relatively low. Specific measures should be taken to effectively control the occurrence and development of adolescent obesity.

Objective: To analyze and evaluate the mental health of students in Inner Mongolia Autonomous Region, so as to provide a reference for conducting mental health education in schools. Methods: A total of 18 867 students aged 9-22 were enrolled from 65 primary and secondary schools and 4 universities in Inner Mongolia that participated in the 2019 National Students Physical Fitness and Health Survey. From September to November 2019, in conjunction with the national student physical health survey, a questionnaire survey of the Kessler Psychological Distress Scale(K10) was carried out. Results: The K10 average score of the survey subjects was (21.96±8.67). The score of the Mongolian nationality(22.57±8.59) was higher than that of the Han nationality(21.53 ±8.70)( t =8.18), the score of rural area (22.42±8.32) was higher than that of the urban area(21.40±9.05)( t = 8.04 ), the primary school students had the lowest score (19.56±8.61) and the senior high school students had the highest score(23.30±8.75)( F =183.26)( P <0.01). In the overall survey subjects, the detection rates of the four levels in good, fair, poor and poor mental health were 25.62%, 28.82%, 26.44% and 19.12%, respectively, and the detection rate of psychological problems was 45.57%. The comparison of the detection rates of mental health problems among attributes showed that the rates of the Han and Mongolian nationalities were 43.27% and 48.85%, respectively( χ 2=101.25); the rates of urban and rural areas were 41.70% and 48.80%, respectively( χ 2=183.44); the rates of the male and the female were 44.88% and 46.26%, respectively( χ 2=19.24)( P < 0.01 ). The prevalence psychological problems among students in primary school, junior high school, senior high school and college were 33.60 %, 49.78%, 52.85% and 47.43%, respectively( χ 2=803.63, P <0.01). Multiple linear regression showed that the detection of psychological problems of Mongolian nationality, urban area, the female and high school students was relatively high. Nationality, urban or rural areas and school stages were the influencing factors of K10 scores[ B(B 95%CI )=0.78(0.05-1.04),0.81( 0.06- 1.06),0.93(0.08-1.03), P <0.05)]. Conclusion Mental health problems are prevalent in adolescents in Inner Mongolia. The government, society, parents and schools should pay more attention to the construction of the software and hardware environment of students mental health, strengthen mental health education and services, and effectively promote the development of students mental health.

Objective: To analyze the development status and sensitive periods of Han and Mongolian students physical fitness development in the Inner Mongolia Autonomous Region, to provide a basis for further research on the growth and development of young students and the selection of athletes. Methods: In 2014, 15 370 Han and Mongolian students from the Inner Mongolia Autonomous Region, ranging in age from 7 to 18 years old, participated in this study. Data on six physical fitness indices, namely grip strength, 50 meters, standing long jump, 800 meters or 1 000 meters, pull-ups/sit-ups in succession, and sit-and-reach were collected. Furthermore, using the average annual growth rate and the standard deviation of half annual growth rate, critical values and the ages at which sensitive periods occur were calculated to examine the differences in the development of various physical qualities between the two ethnic groups. Results: The students showed different growth rates of various indicators at different ages. A sensitive period for strength occurred in Han and Mongolian boys aged 13-15 years old and 12-15 years old, respectively. Sensitive periods for speed occurred in Han and Mongolian boys aged 8-10, 13, and 15 years and 8, 10, and 13-15 years, respectively. Sensitive periods for endurance occurred in Mongolian girls aged 8, 14, and 18 years. Sensitive periods for flexibility occurred in Han girls aged 13 and 15 years old. Conclusion Han and Mongolian students experience sensitive periods for different physical qualities at different ages. Moreover, there are apparent ethnic and gender differences that are retained across age groups and several scattered age points that exist alone or interspersed with each other.

Long non-coding RNA (lncRNA) Dnm3os plays a critical role in peritendinous fibrosis and pulmonary fibrosis, but its role in the process of cardiac fibrosis is still unclear. Therefore, we carried out study by using the myocardial fibrotic tissues obtained by thoracic aortic constriction (TAC) in an early study of our group, and the
Fibroblasts ; Fibrosis ; Humans ; Myocardium/pathology* ; RNA, Long Noncoding ; Signal Transduction ; Transforming Growth Factor beta1

According to the actual requirements of pediatric intensive care, a suction detection system of pediatric oral secretions integrated with monitoring function is designed. The system has the function of adjustable intermittent attraction. The duration and proportion of intermittent attraction can be adjusted according to the individualized needs of pediatric intensive care. The suction head of pacifier can reduce the mechanical damage to pediatric oral mucosa as much as possible. Meanwhile, the system can detect and monitor the real-time biochemical indexes of the collected oral secretions, which can be used to help the judgement of aspiration and quantitatively evaluate the microcirculation dysfunction.
Bodily Secretions ; Child ; Humans ; Mouth ; Suction/instrumentation*

Objective To design and develop an in vitro simulation device for circumferential stress of mural coronary artery, so as to achieve the in vitro loading of mural circumferential stress under coronary myocardial bridge oppression with different degrees. MethodsUsing the in vitro simulation device for myocardial bridge coronary artery hemodynamics, the in vitro measurement of mural circumferential stress was achieved. Based on the experimental data, the in vitro loading of mural circumferential stress under coronary myocardial bridge oppression with different degrees was achieved. Results The in vitro measurement experiment showed that the maximum, average and fluctuation of circumferential stress at proximal end of mural coronary artery would increase significantly with the increase in the degree of myocardial bridge oppression. The in vitro loading experiment of mural circumferential stress verified that the loading waveform coincided basically with the experimental waveform from in vitro measurement. Conclusions The device could realize the in vitro loading of mural circumferential stress, which provided an in vitro simulation platform which was as close as possible to the in vivo environment, so as to explore the influence from hemodynamic abnormality of proximal mural coronary artery on the occurrence of atherosclerosis and plaque rupture.