Objective To study the expression of key regulators of iron metabolism,iron death inhibitor and ferririn heavy chain 1(FTH1),in bladder cancinoma and their relationship with immune invasion and prognosis.Methods Sixty patients diagnosed with bladder cancer at Jiamusi Central Hospital from January 2016 to January 2018 were chosen as the subjects of this study.Collected clinical tissue samples from patients and analyzed the protein expression of FTH1 and the infiltration abundance of three types of immune cells[CD8+T cells,CD4+T cells,natural killer(NK)cells]in bladder tumor samples through immunohistochemistry(IHC).Pearson analyzed the correlation between FTH1 and the expression of immune cell marker.Kaplan-Meier(KM)survival curve was used to analyze the relationship between FTH1 and overall survival(OS),disease free survival(DFS)in bladder cancer patients.COX proportional hazards regression model analyzed risk factors affecting the prognosis and survival of bladder cancer patients.Results The positive expression rate of FTH1 protein in bladder cancer tissue was significantly higher than that in normal adjacent tissues(37.50％vs 12.50％),with a statistically significant difference(χ2=36.391,P<0.05).The positive rate of FTH1 in cancer tissues with female gender,non papillary tumor histological subtype,and T1～T2 lesion infiltration degree,American joint committee on cancer(AJCC)stage I～Ⅱ,was lower than that in cancer tissues with male gender,papillary tumor histological subtype,T3～T4 lesion infiltration degree,and AJCC stage Ⅲ～Ⅳ,and the differences were statistically significant(χ2=4.156～13.846,all P<0.05).The five-year cumulative OS and cumulative DFS of the FTH1 high expression group were significantly lower than those of the FTH1 low expression group,and the differences were statistically significant(Log-rank χ2=25.35,33.67,all P<0.0001).The results of the multivariate COX regression analysis indicated that tumor histological subtype and high expression of FTH1 were identified as independent prognostic risk factors for bladder cancer(all P<0.01).Additionally,a positive correlation was observed between high FTH1 expression in bladder cancer and the IHC score of forkhead box protein P3+(Foxp3+)tumor-infiltrating lymphocytes(TILs)(r=0.580,P<0.05),while negative correlations were found between the IHC scores of CD8+TILs CD56+TILs,and CD4+TILs(r=-0.532,-0.533,-0.452,all P<0.05).Conclusion FTH1 as a biomarker potentiates the prediction of bladder cancer prognosis and the immune micro-environmental(IME).

Objective To study the expression of key regulators of iron metabolism,iron death inhibitor and ferririn heavy chain 1(FTH1),in bladder cancinoma and their relationship with immune invasion and prognosis.Methods Sixty patients diagnosed with bladder cancer at Jiamusi Central Hospital from January 2016 to January 2018 were chosen as the subjects of this study.Collected clinical tissue samples from patients and analyzed the protein expression of FTH1 and the infiltration abundance of three types of immune cells[CD8+T cells,CD4+T cells,natural killer(NK)cells]in bladder tumor samples through immunohistochemistry(IHC).Pearson analyzed the correlation between FTH1 and the expression of immune cell marker.Kaplan-Meier(KM)survival curve was used to analyze the relationship between FTH1 and overall survival(OS),disease free survival(DFS)in bladder cancer patients.COX proportional hazards regression model analyzed risk factors affecting the prognosis and survival of bladder cancer patients.Results The positive expression rate of FTH1 protein in bladder cancer tissue was significantly higher than that in normal adjacent tissues(37.50％vs 12.50％),with a statistically significant difference(χ2=36.391,P<0.05).The positive rate of FTH1 in cancer tissues with female gender,non papillary tumor histological subtype,and T1～T2 lesion infiltration degree,American joint committee on cancer(AJCC)stage I～Ⅱ,was lower than that in cancer tissues with male gender,papillary tumor histological subtype,T3～T4 lesion infiltration degree,and AJCC stage Ⅲ～Ⅳ,and the differences were statistically significant(χ2=4.156～13.846,all P<0.05).The five-year cumulative OS and cumulative DFS of the FTH1 high expression group were significantly lower than those of the FTH1 low expression group,and the differences were statistically significant(Log-rank χ2=25.35,33.67,all P<0.0001).The results of the multivariate COX regression analysis indicated that tumor histological subtype and high expression of FTH1 were identified as independent prognostic risk factors for bladder cancer(all P<0.01).Additionally,a positive correlation was observed between high FTH1 expression in bladder cancer and the IHC score of forkhead box protein P3+(Foxp3+)tumor-infiltrating lymphocytes(TILs)(r=0.580,P<0.05),while negative correlations were found between the IHC scores of CD8+TILs CD56+TILs,and CD4+TILs(r=-0.532,-0.533,-0.452,all P<0.05).Conclusion FTH1 as a biomarker potentiates the prediction of bladder cancer prognosis and the immune micro-environmental(IME).

Objective:To investigate the effect of ultrasound measurement of optic nerve sheath diameter (ONSD) in adult patients with elevated intracranial pressure (ICP).Method:From June 2017 to March 2020, A total of 64 patients (32 patients with elevated ICP and 32 patients with normal ICP) were placed with invasive intracranial pressure monitoring probe in Beijing Pinggu Hospital. Their ICP and ONSD were continuously monitored. Thirty-two healthy volunteers were recruited as control group to check ONSD. The correlation between ONSD and ICP, and the changes of ICP and ONSD after osmotic therapy were observed.Results:The ONSD in ICP increased group was significantly higher than that in normal ICP group: (5.77 ± 0.3) mm vs. (5.01 ± 0.1) mm, with statistical difference ( P<0.05), and there was a positive correlation between ONSD and ICP. There was no significant difference in ONSD between normal ICP group and control group ( P>0.05). Conclusions:Ultrasound monitoring ONSD can reflect the level of ICP and evaluate the effect of osmotic therapy and the prognosis of patients. Bedside ultrasound examination of optic nerve sheath diameter could be used to judge ICP and to evaluate the curative effect of osmotic therapy, with high clinical application value.

Objective: To explore sarcoplasmic reticulum ryanodine receptor2 (RyR 2) expression and calcium releasing function in chronic heart failure (CHF) rabbits and to study the impact of long term valsartan treatment in relevant animals. Methods: HF model was established by volume overloading with pressure overloading in experimental rabbits. 27 rabbits were divided into 3 groups: Sham group, HF group and HF+valsartan group. n=9 in each group and the animals were treated for 7 weeks. Left ventricular structure, hemodynamic parameters, expression and functional changes of myocardiocyte sarcoplasmic reticulum RyR 2 were observed and compared among different groups. Results: Compared with Sham group, HF group had increased left ventricular mess index (LVMI), left ventricular end diastolic pressure (LVEDP) and decreased left ventricular shortening fraction, LVEF, all P<0.05. Compared with HF group, HF+valsartan group showed decreased LVMI, LVEDP and increased left ventricular shortening fraction, LVEF, all P<0.05. Sarcoplasmic reticulum RyR 2 expression and calcium releasing function were lower in HF group than Sham group, P<0.05; while they were both higher in HF+valsartan group than HF group, P<0.05. Conclusion: Long term application of valsartan could improve the cardiac function which might be related to increased myocardial sarcoplasmic reticulum RyR 2 expression and calcium releasing function in experimental CHF rabbits.

Objective: To explore the changes of protein expression and activity of calcium/calmodulin-dependent protein kinase-II (CaMK-II) in myocardium nucleus and sarcoplasmic reticulum in experimental rabbits with heart failure (HF).
Methods: A total of 16 rabbits were divided into 2 groups: Sham group and HF group, the HF model was established by volume overload plus pressure overload.n=8 in each group and all animals were treated for 7 weeks. Left ventricular structure, hemodynamic parameters and protein expression and activity of CaMK-II in myocardium nucleus and sarcoplasmic reticulum were examined and compared between 2 groups.
Results: Compared with Sham group, HF group presented increased left ventricular mass index (LVMI) (1.32 ± 0.06) g/kg vs (3.61 ± 0.09) g/kg, LVEDP (-1.50 ± 0.50) mmHg vs (23.00 ± 2.37) mmHg, allP<0.05; while decreased left ventricular shorten fraction (37.83 ± 3.58) % vs (17.38 ± 3.13) % and LVEF (71.92 ± 4.56) % vs (38.50 ± 6.07) %, allP<0.05. The protein expression and activity of CaMK-II were both higher in HF group than Sham group, allP<0.05.
Conclusion: Increased protein expression and activity of CaMK-II in myocardium nucleus and sarcoplasmic reticulum might be one of the mechanisms for HF occurrence in experimental rabbits.

Object To study the preparation and technology on sinomenine (SM) release from Sinomenine Sustained-release Tablets (SSTs) in which hydroxypropyl methyl cellulose (HPMC) was used as the primary excipients. Methods SSTs were prepared with different HPMC viscosity of K4M, K15M, and K100M, different HPMC content, and preparing technology. Results Little effect was observed on the releasing rate of SM with different HPMC viscosity when the content of HPMC was 30%. SM releasing rate increased with the decreasing of proportion of HPMC while the content of HPMC was less than 30%. But the releasing velocity slowed down while the content of HPMC increased and the effect on the releasing rate was not found as the content of HPMC was over 30%. When the ratio of SM and HPMC was 1∶1.5, the releasing rate decreased with the increasing of tablet weight from 280 mg to 360 mg. The releasing rate was insensitive to the particle size of HPMC and hardness of SSTs in this study. Conclusion It is necessary to control the tablet weight and choose the proper quantity of HPMC in the preparation of SSTs.