Objective:To investigate impacts of paeoniflorin on inflammation and nuclear transcription factor-κB(NF-κB)/nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)signal pathway in knee osteoarthritis(KOA)model rats.Meth-ods:SD rats were randomly grouped into model group,glucosamine group,paeoniflorin low-dose group,paeoniflorin high-dose group,paeoniflorin high-dose+phorbol ester(PMA)(NF-κB activator)group,with 10 rats in each group,another 10 rats were regarded as control group,and only knee joint capsule was cut,after treatment with glucosamine,paeoniflorin and PMA,joint pain symptoms of rats were detected by mechanical stimulation method and thermal radiation method;knee joint width,joint swelling and synovial thick-ness were measured;HE staining was applied to detect joint tissue structure and morphology of rats in each group;levels of inflamma-tory factors IL-1β,monocyte chemoattractant protein 1(MCP-1)and IL-9 in joint fluid and serum of rats were detected by ELISA;and Western blot was applied to detect expressions of NF-κB/NLRP3 signal pathway related proteins in rat joint tissue.Results:Com-pared with control group,joint tissue structure of model group was significantly damaged,mechanical pain threshold and thermal sen-sitivity pain threshold were significantly lower(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were higher(P<0.05).Compared with model group,joint tissue damage of rats in glucosamine group,paeoniflorin low-dose group and paeoniflorin high-dose group was reduced,mechanical pain threshold and thermal sensitivity pain threshold were higher(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expressions of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were lower(P<0.05);joint tissue injury of rats in paeoniflorin high-dose group was further reduced compared with paeoniflorin low-dose group,mechanical pain threshold and thermal sensitivity pain threshold were further higher(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were further lower(P<0.05).Compared with paeoniflorin high-dose group,joint tissue damage of rats in paeoniflorin high-dose+PMA group was increased,mechanical pain threshold and thermal sensitivity pain threshold were lower(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were higher(P<0.05);there was no significant change in all indexes of rats in glucosamine group(P>0.05).Conclusion:Paeoniflorin can play an anti-inflammatory role by down-regulating the NF-κB/NLRP3 signal pathway,thus alleviating joint injury and joint pain symptoms of KOA rats.

Objective:To investigate impacts of paeoniflorin on inflammation and nuclear transcription factor-κB(NF-κB)/nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)signal pathway in knee osteoarthritis(KOA)model rats.Meth-ods:SD rats were randomly grouped into model group,glucosamine group,paeoniflorin low-dose group,paeoniflorin high-dose group,paeoniflorin high-dose+phorbol ester(PMA)(NF-κB activator)group,with 10 rats in each group,another 10 rats were regarded as control group,and only knee joint capsule was cut,after treatment with glucosamine,paeoniflorin and PMA,joint pain symptoms of rats were detected by mechanical stimulation method and thermal radiation method;knee joint width,joint swelling and synovial thick-ness were measured;HE staining was applied to detect joint tissue structure and morphology of rats in each group;levels of inflamma-tory factors IL-1β,monocyte chemoattractant protein 1(MCP-1)and IL-9 in joint fluid and serum of rats were detected by ELISA;and Western blot was applied to detect expressions of NF-κB/NLRP3 signal pathway related proteins in rat joint tissue.Results:Com-pared with control group,joint tissue structure of model group was significantly damaged,mechanical pain threshold and thermal sen-sitivity pain threshold were significantly lower(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were higher(P<0.05).Compared with model group,joint tissue damage of rats in glucosamine group,paeoniflorin low-dose group and paeoniflorin high-dose group was reduced,mechanical pain threshold and thermal sensitivity pain threshold were higher(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expressions of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were lower(P<0.05);joint tissue injury of rats in paeoniflorin high-dose group was further reduced compared with paeoniflorin low-dose group,mechanical pain threshold and thermal sensitivity pain threshold were further higher(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were further lower(P<0.05).Compared with paeoniflorin high-dose group,joint tissue damage of rats in paeoniflorin high-dose+PMA group was increased,mechanical pain threshold and thermal sensitivity pain threshold were lower(P<0.05),knee joint width,joint swelling and synovial thickness,levels of IL-1β,MCP-1 and IL-9 in joint fluid and serum,and expression of p-NF-κB p65/NF-κB p65 and NLRP3 proteins in joint tissue were higher(P<0.05);there was no significant change in all indexes of rats in glucosamine group(P>0.05).Conclusion:Paeoniflorin can play an anti-inflammatory role by down-regulating the NF-κB/NLRP3 signal pathway,thus alleviating joint injury and joint pain symptoms of KOA rats.

Objective To investigate the efficacy evaluation of early continuous renal replacement therapy (CRRT) for severe acute pancreatitis in intensive care unit (ICU).Methods Prospective study was performed for 84 patients with severe acute pancreatitis admitted to the Xinjiang Provincial People's Hospital from June 2015 to March 2017.Those patients were divided into two groups by computer random coding:42 cases treated with CRRT (observation group), and 42 cases received routine treatment (control group).Statistically analysis was carried out between two groups regarding curative effect, symptom improvement, ICU hospitalization time, and serum markers before and after treatment.Results The total ef fective rate was 92.86 ％ in the observation group, and 85.71％ in the control group, without significant difference between two groups (F =0.516, P =0.632).Patients with symptoms, vital signs stable time, gastrointestinal function recovery time, and hospitalization time of ICU were significantly lower in the observation group than the control group [(3.16 ± 1.28) days:(5.21 ± 1.45) days, t =3.518, P =0.017, (2.55 ± 1.36) days:(4.34 ± 1.51) days, t =2.519, P =0.034, (4.46 ± 1.28) days:(6.28 ± 1.51) days, t=2.685,P=0.028, (15.06±2.24)days:(19.34 ±3.28) days, t=6.983, P=0.009].There is no significant differences in amylase (AMS), interleukin (IL)-6, C-reaction protein (CRP), tumor necrosis factor (TNF)α, prothrombin time (PT) and fibrinogen (FIB) before treatment between two groups (P ＞ 0.05).The observation group were significantly lower than the control group in AMS, IL-6, CRP, TNF-α, PT, and FIB levels after treatment [(206.59 ± 19.51) U/L:(258.12 ± 22.43) U/L, t =11.234, P=0.001, (34.58 ±6.41)ng/L:(41.36 ± 8.52) ng/L, t =4.121,P =0.013, (88.24 ± 6.95) ng/L:(104.33 ±10.82) ng/L, t=8.109,P=0.002, (178.35 ±27.43)pg/ml:(249.28 ±34.33)pg/ml, t=7.384,P=0.007, (12.48±3.54)s:(14.56 ±4.62)s, t =6.473,P=0.011, (3.38±1.55)g/L:(4.57 ± 2.86)g/L, t =4.108, P =0.041].Two groups of patients after treatment were cured, and no serious complications occurred during hospitalization.Conclusions For patients with severe acute pancreatitis, the overall effective rate of CRRT in ICU was high, the clinical symptoms relieved quickly, the level of serum indicators improved effectively.

Objective To observe the effect of "Jingu Tongxiao Wan" on blood stasis and cold coagulation type gonarthrosis.Methods 160 patients with blood stasis and cold coagulation type gonarthrosis were divided into the "Jingu Tongxiao Wan" group(group A)with 120 cases(treated with "Jingu Tongxiao Wan" 6 g/time,2 times/d)and "Guci Pian" group(group B)with 40 cases(treated with "Guci Pian" 3 tablets/time,3 times/d).Indexes of effect and safe were observed after one month.Results 23 cases were healed,63 cases were excellent,9 cases were utilitied,9 cases were invalid,total effective rate was 93.8% in the group A.While,in the group B,7 cases were healed,21 cases were excellent,9 cases were utilitied,3 cases were invalid,total effective rate was 92.5%.There was no significant difference between two groups(P>0.05).Scores of sings and symptoms before and after treatment were 8.98±2.24 and 2.15±1.71 respectively in the group A,and 8.29±2.35 and 2.25±1.25 respectively in the group B.The scores after treatment were significantly different from that before treatment in each group(P<0.01),and no significant difference between scores of the group A and group B after treatment(P>0.05).Conclusion The treatment of "Jingu Tongxiao Wan" shows an obvious effect and safe on blood stasis and cold coagulation type gonarthrosis.

BACKGROUND: The morbidity of cervical spondylosis complicated with osteoporosis is rather high in senile patients, which affects the life quality.OBJECTIVE: To observe the therapeutic effects of gushukang, the Chinese herbal patent for kidney tonification on senile cervical spondylosis complicated with osteoporosis.DESIGN: Random control study based on patients.SETTING: Orthopedics department in a provincial hospital of traditional Chinese medicine.PARTICIPANTS: Fifty cases of senile cervical spondylosis complicated with osteoporosis collected from the inpatients and outpatients in Henan Hospital of Traditional Chinese Medicine from .June 2002 to December 2003.METHODS: Fifty cases of senile cervical spondylosis complicated with osteoporosis were randomized into experimental group(30 cases) and the control(20 cases) . In experimental group, gushukang was prescribed, 10 g/time and twice a day. In the control, jingfukang was prescribed, 6 g/time,twice per day. The course of treatment was 6 months.MAIN OUTCOME MEASURES: To observe the improvement of therapeutic effects, X-ray manifestation, bone density, serum calcium and phosphorus before and after treatment in two groups.RESULTS: The therapeutic effects were obtained in both groups. The effective rate was 93％ in treatment group, better than the control (75％) ( P ＜ 0.05). Concerning to remote ultrasonic conductive speed in radius(SOS), it was(3 934 ± 125) and(3 990 ± 105) m/s before and after treatment respectively in experimental group; and was(3 935 ± 110) and(3 940 ± 123) m/s in the control, indicating significant difference( P ＜ 0. 05) . The difference in SOS before and after treatment in the control was not significant ( P ＞ 0.05).There was no remarkable difference in serum calcium and phosphorus before and after treatment in two groups( P ＞ 0.05).CONCLUSION: Gushukang, Chinese herbal patent for kidney tonification,provides better therapeutic effects on senile cervical spondylosis complicated with osteoporosis.