Objective:To explore the effect of combining hyperbaric oxygen inhalation with wet healing in the treatment of chronic refractory ulcers of the skin.Methods:Sixty male Sprague-Dawley rats were randomly formed into a general treatment group (group A), a wet healing group (group B), and a hyperbaric oxygen + wet healing group (group C), each of 20. Chronic and refractory skin ulcers were induced on all of the rats after one week of routine feeding and 6 hours of fasting. Six courses (once daily for 10 days/course)of the three treatments were then administered. After 2, 4 and 6 courses of treatment the wound healing rate and recovery were analyzed using histopathological methods.Results:After 2, 4 and 6 courses of treatment, the average wound healing rates of groups B and C were significantly higher than group A′s average, and that of group C was significantly better than that of group B. After 6 courses of treatment, group C′s average histopathology score was significantly higher than that of group B, and those of groups B and C were both significantly higher than group A′s average.Conclusion:Single wet healing is better than common treatment for chronic skin ulcers, but hyperbaric oxygen inhalation can further improve its effectiveness.

Objective: To systematically evaluate the effects of hyperbaric oxygen on the hemodynamics and intracranial pressure of patients with severe craniocerebral injury (STBI). Methods: Reports of randomized and controlled trials applying hyperbaric oxygen in the treatment of STBI were retrieved from the Pubmed, Cochrane Library, Embase, CBM, CNKI, VIP and Wan Fang databases. Each report found was evaluated by two researchers independently applying pre-defined inclusion and exclusion criteria. The data were extracted and combined and a meta-analysis was performed. Results: Eight trials involving 725 patients were included in the meta-analysis. They combined to demonstrate that intracranial pressure, oxygen uptake and scores on the Glasgow coma scale improved significantly more in the hyperbaric oxygen group than in the control group after between 3 and 10 days of treatment. Conclusion Hyperbaric oxygen therapy is effective in treating severe craniocerebral injury and it is worthy of clinical application.

Objective To observe the effect of hyperbaric oxygen (HBO) therapy on neurological functioning in rats modelling cerebral hemorrhage (ICH).Methods Sixty Sprague-Dawley rats had intracerebral hemorrhage induced by injecting autologous blood.They were then randomly divided into an HBO-free group and an HBO group,each of 30 according to a random number table.The HBO group was further divided into HBO 3 h,HBO 6 h,HBO 1 d,HBO 2 d and HBO 7 d groups which received HBO therapy for 3 hours,6 hours,1 day,2 days and 7 days respectively.Each had 6 members.The HBO-free rats were also divided into analogous HBO-free 3 h,HBO-free 6 h,HBO-free 1 d,HBO-free 2 d and HBO-free 7 d groups,and give no HBO intervention.All of the rats were evaluated for neurological impairment using the Longa scoring method before the treatment and 10 days,20 days and 30 days afterward.Results After 10,20 and 30 days of HBO treatment,there were significant differences in neurological functioning between each pair of HBO-free and HBO-treated groups.After 10 and 20 days of HBO treatment the average neurological function score of the HBO 3 h group was significantly different from that of the HBO 2 d group.The average score in the HBO 7 d group was also significantly different from that of the HBO 3 h,HBO 6 h,HBO 1 d and HBO 2 d groups after 10,20 and 30 days of HBO treatment.The average scores of the HBO 3 h,HBO 6 h,HBO 1 d and HBO 2 d groups improved significantly between 10 and 20 days after the treatment.The average score of the rats which received 30 days of treatment was also significantly different from those after 10 and 20 days.Conclusion HBO treatment can improve neurological function after cerebral hemorrhage,at least in rats.The best time to start HBO treatment is no later than 24 hours after the hemorrhage.The curative effect increases with extension of the treatment's duration.

Objective To assess systematically the efficacy and safety of hyperbaric oxygen (HBO)combined with drugs in treatment of radiation encephalopathy (PREP).Methods Computer-aided information retrieval was made by using such databases as Pubmed,Cochrane Library,Embase,CBM,CNKI,VIP and WanFang data,at the same time,manual data retrieval was also made by searching related literature both at home and abroad.The time span for information retrieval was from the inception of the above-mentioned database to March 2018.After 2 reviewers independently screened the retrieved literature in accordance with the included and excluded criteria,and collected the retrieved papers and evaluated the methodological quality of the retrieved literature,meta-analysis was performed by using RevMan 5.2 software.Results Finally,a total of 16 randomized controlled trials (RCTs) involving 990 patients were included in the study.Meta analysis showed that effective rate and cure rate of the HBO combined with drugs group were respectively [OR =5.78,95％ CI (4.15,8.06)] (P＜0.01) and [OR=4.56,95c％CI (2.88,7.20)] (P＜0.01),which were superior to those of the simple drug treatment group.And the number of foci was less than that of the simple drug treatment group [OR =-2.63,95％ CI (-3.70,-1.56)] (P ＜ 0.01).Conclusion Current evidence shows that HBO combined with drug treatment could produce good effects on radiation encephalopathy.For this reason,it is worth further clinical popularization.

Objective To investigate the clinical effects of hyperbaric oxygen (HBO) combined with Yangxueqingnao granules on tinnitus and dizziness in patients with sudden deafness.Methods One hundred and twenty-four patients admitted into the Sichuan Provincial People's Hospital from March 2015 to June 2016 were randomly assigned to the observation group (n =61) and the control group (n =63) by using the random digital software to generate random number and opaque envelope hidden test scheme.The control group only received routine therapy,including improvement of circulation,nerve nutrition,hormone and symptomatic treatment,while the observation group was supplemented with HBO + Yangxueqingnao granules in addition to routine therapy.The observation group received HBO therapy one session a day,5 days a week and 10 days a treatment course.In addition,the patients in the observation group had Yangxueqingnao granules orally,one pack once and 3 times a day.After 2 weeks of treatment,therapeutic efficacy of the 2 groups was evaluated by the blinded method,and after 3 and 6 months of treatment,therapeutic effects on tinnitus and dizziness of the 2groups were evaluated through medical follow-ups by telephone.Results All the patients of the 2 groups had tinnitus,the observation group had 42 cases of dizziness,while the control group had 36 cases of dizziness.Following treatment for 2 weeks,3 and 6 months,total effective rates of tinnitus in the observation group were respectively 83.61％,75.41％ and 77.05％,which were all higher than those of the control group (65.08％,57.14％,58.73％),and statistical significance could be noted when comparisons were made between the 2 groups (P ＜ 0.05).Total effective rate of dizziness in the observation group after 2 weeks,3 and 6 months of treatment was 95.23％,which was obviously better than those of the control group (75.00％,80.56％,83.33％),and statistical significance could also be seen when comparisons were made between the 2 groups (P ＜ 0.05).Conclusions HBO combined with Yangxueqingnao granules in the treatment of patients with sudden deafness complicated with tinnitus and dizziness could produce good clinical effects.For this reason it was worth further clinical promotion.

Objective To assess systematically the efficacy and safety of hyperbaric oxygen (HBO)combined with drugs in treatment of radiation encephalopathy (PREP).Methods Computer-aided information retrieval was made by using such databases as Pubmed,Cochrane Library,Embase,CBM,CNKI,VIP and WanFang data,at the same time,manual data retrieval was also made by searching related literature both at home and abroad.The time span for information retrieval was from the inception of the above-mentioned database to March 2018.After 2 reviewers independently screened the retrieved literature in accordance with the included and excluded criteria,and collected the retrieved papers and evaluated the methodological quality of the retrieved literature,meta-analysis was performed by using RevMan 5.2 software.Results Finally,a total of 16 randomized controlled trials (RCTs) involving 990 patients were included in the study.Meta analysis showed that effective rate and cure rate of the HBO combined with drugs group were respectively [OR =5.78,95％ CI (4.15,8.06)] (P＜0.01) and [OR=4.56,95c％CI (2.88,7.20)] (P＜0.01),which were superior to those of the simple drug treatment group.And the number of foci was less than that of the simple drug treatment group [OR =-2.63,95％ CI (-3.70,-1.56)] (P ＜ 0.01).Conclusion Current evidence shows that HBO combined with drug treatment could produce good effects on radiation encephalopathy.For this reason,it is worth further clinical popularization.

Objective To investigate the clinical effects of hyperbaric oxygen (HBO) combined with Yangxueqingnao granules on tinnitus and dizziness in patients with sudden deafness.Methods One hundred and twenty-four patients admitted into the Sichuan Provincial People's Hospital from March 2015 to June 2016 were randomly assigned to the observation group (n =61) and the control group (n =63) by using the random digital software to generate random number and opaque envelope hidden test scheme.The control group only received routine therapy,including improvement of circulation,nerve nutrition,hormone and symptomatic treatment,while the observation group was supplemented with HBO + Yangxueqingnao granules in addition to routine therapy.The observation group received HBO therapy one session a day,5 days a week and 10 days a treatment course.In addition,the patients in the observation group had Yangxueqingnao granules orally,one pack once and 3 times a day.After 2 weeks of treatment,therapeutic efficacy of the 2 groups was evaluated by the blinded method,and after 3 and 6 months of treatment,therapeutic effects on tinnitus and dizziness of the 2groups were evaluated through medical follow-ups by telephone.Results All the patients of the 2 groups had tinnitus,the observation group had 42 cases of dizziness,while the control group had 36 cases of dizziness.Following treatment for 2 weeks,3 and 6 months,total effective rates of tinnitus in the observation group were respectively 83.61％,75.41％ and 77.05％,which were all higher than those of the control group (65.08％,57.14％,58.73％),and statistical significance could be noted when comparisons were made between the 2 groups (P ＜ 0.05).Total effective rate of dizziness in the observation group after 2 weeks,3 and 6 months of treatment was 95.23％,which was obviously better than those of the control group (75.00％,80.56％,83.33％),and statistical significance could also be seen when comparisons were made between the 2 groups (P ＜ 0.05).Conclusions HBO combined with Yangxueqingnao granules in the treatment of patients with sudden deafness complicated with tinnitus and dizziness could produce good clinical effects.For this reason it was worth further clinical promotion.

Objective To investigate the effect of hyperbaric oxygen (HBO) at different intervention time with different treatment courses on cerebral ischemia in rats with middle cerebral artery occlusion (MCAO),so as to provide clues and evidences for further exploration of HBO in the treatment of hypoxicischemic encephalopathy.Methods Sixty SD rats with MCAO were divided into 2 groups:the control group that did not receive HBO therapy and the observation group that received HBO therapy.The 2 groups of MCAO rats were divided into 5 subgroups in accordance with different intervention time,i.e.the MCAO 3 h group,the MCAO 6 h group,the MCAO 24 h group,the MCAO 4 d group,and the MCAO 7 d group,each consisting of 6 rats.The animals in the observation group received HBO therapy once a day,10 days a treatment course.Effects of HBO were observed at 4 different time points:before treatment,at day 10,day 20 and day 30 after treatment.Rogers DC and Hunter AJ 7-point scoring method was used to evaluate the treatment and observe the neurological function of the rats.Results (1) With regard to different intervention time,statistical significance could be observed in the neurological function scores of the HBO 30 d observation subgroup,as compared with those before treatment,10 and 20 days after HBO therapy (P ＜0.05).(2)With regard to different treatment courses,Statistical significance could be noted in the neurological function scores(2.67 ± 0.12,3.50 ± 0.41,4.17±0.26) for the MCAO3h,MCAO6h and MCAO24h observation subgroups at day 10 after treatment,as compared with those of the control group(5.67 ± 0.32,5.50 ± 0.21,5.83 ± 0.11) (P ＜ 0.05).Statistical significance was noted in the neurological function scores(2.17 ±0.15,3.00 ±0.33,3.83 ±0.25,6.00 ± 0.34) for the MCAO 3 h,MCAO 6 h,MCAO 24 h and MCAO 4 d observation subgroups at day 20 after treatment,as compared with those of the control group(6.67 ±0.11,6.17 ±0.23,6.17 ±0.13,6.67 ±0.19) (P ＜0.05).Statistical significance could also be seen in the neurological function scores(1.67 ±0.18、2.17±0.21、3.67 ±0.28、6.33 ±0.32) for the MCAO 3 h,MCAO 6 h,MCAO 24 h and MCAO 4 d observation subgroups at dav 30 after treatment,as compared with those of the control group (6.83 ± 0.16、6.67 ± 0.12、6.50 ± 0.23、6.83 ± 0.15) (P ＜ 0.05).Conclusions HBO therapy could significantly decrease the neurological function scores and improve the neurological function of the MCAO rats.The earlier the intervention time of HBO and longer the treatment course,the less severe neurological function lesion would be sustained in the MCAO rats.

Objective To investigate the effect of hyperbaric oxygen (HBO) at different intervention time with different treatment courses on cerebral ischemia in rats with middle cerebral artery occlusion (MCAO),so as to provide clues and evidences for further exploration of HBO in the treatment of hypoxicischemic encephalopathy.Methods Sixty SD rats with MCAO were divided into 2 groups:the control group that did not receive HBO therapy and the observation group that received HBO therapy.The 2 groups of MCAO rats were divided into 5 subgroups in accordance with different intervention time,i.e.the MCAO 3 h group,the MCAO 6 h group,the MCAO 24 h group,the MCAO 4 d group,and the MCAO 7 d group,each consisting of 6 rats.The animals in the observation group received HBO therapy once a day,10 days a treatment course.Effects of HBO were observed at 4 different time points:before treatment,at day 10,day 20 and day 30 after treatment.Rogers DC and Hunter AJ 7-point scoring method was used to evaluate the treatment and observe the neurological function of the rats.Results (1) With regard to different intervention time,statistical significance could be observed in the neurological function scores of the HBO 30 d observation subgroup,as compared with those before treatment,10 and 20 days after HBO therapy (P ＜0.05).(2)With regard to different treatment courses,Statistical significance could be noted in the neurological function scores(2.67 ± 0.12,3.50 ± 0.41,4.17±0.26) for the MCAO3h,MCAO6h and MCAO24h observation subgroups at day 10 after treatment,as compared with those of the control group(5.67 ± 0.32,5.50 ± 0.21,5.83 ± 0.11) (P ＜ 0.05).Statistical significance was noted in the neurological function scores(2.17 ±0.15,3.00 ±0.33,3.83 ±0.25,6.00 ± 0.34) for the MCAO 3 h,MCAO 6 h,MCAO 24 h and MCAO 4 d observation subgroups at day 20 after treatment,as compared with those of the control group(6.67 ±0.11,6.17 ±0.23,6.17 ±0.13,6.67 ±0.19) (P ＜0.05).Statistical significance could also be seen in the neurological function scores(1.67 ±0.18、2.17±0.21、3.67 ±0.28、6.33 ±0.32) for the MCAO 3 h,MCAO 6 h,MCAO 24 h and MCAO 4 d observation subgroups at dav 30 after treatment,as compared with those of the control group (6.83 ± 0.16、6.67 ± 0.12、6.50 ± 0.23、6.83 ± 0.15) (P ＜ 0.05).Conclusions HBO therapy could significantly decrease the neurological function scores and improve the neurological function of the MCAO rats.The earlier the intervention time of HBO and longer the treatment course,the less severe neurological function lesion would be sustained in the MCAO rats.

Objective To explore the influence of hyperbaric oxygen therapy (HBOT) on the learning and memory ability using a rat model of vascular dementia (VD).Methods Sixty Sprague-Dawley rats were selected and divided into a normal group,a sham operation group,a VD group and an HBOT group,each of 15 rats using a random number table.The improved four-vessel method was used to establish a VD model in all except the rats in the normal group,but for the sham operation group,the vertebral artery was not subjected to firing and the arteria carotis communis was not clipped.The HBOT group was then given HBOT for 7 days.The Morris water maze test was used to evaluate their learning and memory ability of all 4 groups.Results Compared to the normal group,the mean escape latency of the sham operation group,the VD group and the HBOT group was significantly longer in each case (P＜0.05).Their platform quadrant crossing times were also significantly less,and their quadrant swimming distance as a proportion of their total swimming distance was significantly smaller (P＜0.05).Compared with the sham operation group,the mean escape latency of the VD and HBOT groups was significantly longer (P＜0.05),the times of crossing platform quadrant were less and their quadrant swimming distance was a smaller proportion of their total swimming distance.As compared with the VD group,the mean escape latency of the HBOT group was significantly shorter [(25.73±6.20) s],the average platform quadrant crossing times were significantly more [(9.51±2.25) times] and the platform quadrant swimming distance of the HBOT group was significantly longer [(494.72±208.26)],representing a significantly larger proportion of the total swimming distance [(28.71 ± 5.32) ％] (P＜0.05).Conclusion HBOT can effectively improve learning and memory in vascular dementia,at least in rats.