1.Exploratory Study on the Impact of Intestinal Fungi on the Progression of Heart Failure in Patients with Chronic Kidney Disease
Shuting LI ; Lili DONG ; Xinlei YANG ; Lin LYU ; Fukai LIU
Medical Journal of Peking Union Medical College Hospital 2025;16(5):1236-1243
To investigate the role of intestinal fungi in the progression of heart failure (HF) associated with chronic kidney disease (CKD). This study consisted of two parts. The first part was a clinical study. Fecal samples from CKD patients (CKD group), CKD patients with HF (CKD+HF group), and healthy individuals (healthy control group) were subjected to 18S rRNA sequencing to compare differences in intestinal fungal microbiota among the three groups. The second part was an animal experiment. Male C57BL/6J mice were randomly divided into a control group (fed a standard diet), a CKD group (fed a 0.2% adenine diet), and a CKD+amphotericin B group (fed a 0.2% adenine diet+0.5 mg/L amphotericin B in drinking water), with 10 mice in each group. After successful modeling, cardiac function and histomorphological differences among the three groups were compared by assessing exercise tolerance, left ventricular ejection fraction (LVEF), left ventricular shortening fraction (LVFS), serum N-terminal pro-brain natriuretic peptide (NT-proBNP) levels, and histological examinations (HE staining and Masson staining) of cardiac tissue. The clinical study revealed that compared with the healthy control group ( Intestinal fungi may be associated with the progression of HF in CKD patients. Depletion of intestinal fungi could potentially ameliorate cardiac remodeling and delay the onset and progression of HF. Intestinal fungi may serve as a novel therapeutic target for HF in CKD patients.
2.Analysis of 166 cases of adverse transfusion reactions in children
Kexuan QU ; Fukai BAO ; Aihua LIU
International Journal of Laboratory Medicine 2017;38(1):39-40,43
Objective To analyze the causes of blood transfusion adverse reactions in children,and to explore the measures to reduce the adverse reactions of blood transfusion in children.Methods Collect 30 518 cases of transfusion reaction data which is during 2009 to 2014 in Kunming Children's hospital for analyzing.Results 166 cases of transfusion reaction occurred and the occurrence rate is 0.54％ in 30 518 cases,allergic transfusion reaction accounted for 89.16％ (148/166),non-hemolytic febrile reaction 10.24 ％ (17/166),hemoglobinuria accounted for 0.60 ％ (1/166);In children the transfusion reaction rate of hemapheresis platelet is 1.92％(72/3 743),plasma is 0.43％(56/13 132),suspension red blood cells is 0.28％(38/13 480);the signs and symptoms of children's blood transfusion reaction:simplex urticaria and rash 74.10％(123/166),simplex fever 10.24％ (17/166),rash associated with fever 5.42％ (9/166),eyelid or oral edema (mild angioneurotic oedema) 9.04％ (15/166),,Bronchospasm (mild) 0.60％ (1/166),simple hemoglobinuria 0.60％ (1/166).Conclusion Children with blood transfusion adverse reactions to allergies,mainly for local or systemic rash;Platelet is the most-common blood component that causes transfusion reaction with children.
3.Principle and Application Progress of CRISPR-Cas9
Jiaru YANG ; Meihua GUO ; Aihua LIU ; Fukai BAO
Journal of Kunming Medical University 2016;37(5):118-122
Recently,a fast developing new technology for gene modification named as CRISPR-Cas9 which based on CRISPR-Cas9 system composed of clustered regulatory interspaced short palindromic repeat(CRISPR) and Cas9 nuclease(CRISPR associated system 9,Cas9)has been developed. CRISPR-Cas9 system is a kind of immune mechanism widely found in bacteria and archaea. This mechanism can help bacteria and archaea against exogenous DNA by the approach of specifically breaking DNA. Later,this mechanism was found to be useful for gene modification and gene deletion. At present,this technology has been applied to gene modification and therapy. Many studies have shown that the technology,compared with other genetic technology,has higher efficiency and accuracy,and it has promoted genetic engineering progress. Summarized here is the principle and application advance of CRISPR-Cas9.
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