Objective:By observing the loss(LORR)and recovery of righting reflex(RORR)in mice after anes-thesia and the exploration of Y-maze,explore the role of oxytocin(OXT)in promoting arousal in mice.Methods:The expression of oxytocin receptor(OXTR)in the cell bodies and tyrosine hydroxylase(TH)positive immunoreactive neu-rons in locus coeruleus(LC)was verified by immunofluorescence staining.Using the function of intraperitoneal injec-tion anesthesia and gas anesthesia,and intraperitoneal injection,intrathecal injection,and right LC single tube catheter administration,OXT or saline given as experimental group and control group,respectively,explored the time needed in the induction anesthesia period and anesthesia recovery period of mice after administration,and conducted Y-maze test on groups with statistically different anesthesia results,then explored the exploration of novel arm in Y-maze after 6,9,18 and 24 hours of RORR.Results:In LC,TH and OXTR positive neurons had double labeling.There was no differ-ence in the time of RORR after intraperitoneal injection and intrathecal injection of OXT and saline respectively,and no difference in the time of LORR and RORR after single-tube cannula administration of OXT and saline with intraperitone-al anesthesia and gas anesthesia.Compared with the control group,the recovery time of mice in the experimental group under gas anesthesia given OXT with single cannula was reduced(P＜0.05).In this group,the exploration activities of Y-maze novel arm increased after 9 hours of anesthesia recovery(P＜0.05),but there was no significant statistical difference in the exploration activities of Y-maze novel arm at 6,18 and 24 hours.Western Blot showed that compared with the control group,the expression of TH in LC in the experimental group increased significantly within 9 hours after anesthesia(P＜0.05),and then began to decrease after 9 hours(P＜0.01).Conclusion:OXT can promote the arousal of mice,and the cognitive degree of mice given OXT recovered better after waking up for 9 hours after anesthe-sia.

Objective:By observing the loss(LORR)and recovery of righting reflex(RORR)in mice after anes-thesia and the exploration of Y-maze,explore the role of oxytocin(OXT)in promoting arousal in mice.Methods:The expression of oxytocin receptor(OXTR)in the cell bodies and tyrosine hydroxylase(TH)positive immunoreactive neu-rons in locus coeruleus(LC)was verified by immunofluorescence staining.Using the function of intraperitoneal injec-tion anesthesia and gas anesthesia,and intraperitoneal injection,intrathecal injection,and right LC single tube catheter administration,OXT or saline given as experimental group and control group,respectively,explored the time needed in the induction anesthesia period and anesthesia recovery period of mice after administration,and conducted Y-maze test on groups with statistically different anesthesia results,then explored the exploration of novel arm in Y-maze after 6,9,18 and 24 hours of RORR.Results:In LC,TH and OXTR positive neurons had double labeling.There was no differ-ence in the time of RORR after intraperitoneal injection and intrathecal injection of OXT and saline respectively,and no difference in the time of LORR and RORR after single-tube cannula administration of OXT and saline with intraperitone-al anesthesia and gas anesthesia.Compared with the control group,the recovery time of mice in the experimental group under gas anesthesia given OXT with single cannula was reduced(P＜0.05).In this group,the exploration activities of Y-maze novel arm increased after 9 hours of anesthesia recovery(P＜0.05),but there was no significant statistical difference in the exploration activities of Y-maze novel arm at 6,18 and 24 hours.Western Blot showed that compared with the control group,the expression of TH in LC in the experimental group increased significantly within 9 hours after anesthesia(P＜0.05),and then began to decrease after 9 hours(P＜0.01).Conclusion:OXT can promote the arousal of mice,and the cognitive degree of mice given OXT recovered better after waking up for 9 hours after anesthe-sia.

Objective To explore the application effect of personalized lateral decubitus pillow for patients with cervical spine surgery. Methods A totals of 106 patients after cervical spine surgery were recruited and randomly divided into control group (48 cases) and experimental group (58 cases). Patients in the control group used the common pillow when patients lay lateral position-the height of pillow equal to shoulder cheekbone breadth, while experimental group used the personalized lateral decubitus pillow which supported suspended occipitalis and the height of pillow adjusted by individual. The comfort score, incision pain score and last time for lateral position of all patients were compared. Results The comfort score of the experimental group was significantly higher than that of the control group during the first day, second day and the third day after surgery [(66. 3 ± 4. 8) vs (63. 4 ± 3. 5), (68. 4 ± 5. 3) vs (65. 1 ± 4. 6), (71. 3 ± 4. 8) vs (68. 3 ± 4. 4);t=3. 461,3. 473,3. 270, respectively; P <0. 05]. The incision pain score of the experimental group was significantly lower than that of the control group during the first day, second day and the third day after surgery [(5.09±1.33) vs(5.88±1.29),(4.78±1.30) vs(5.52±1.09),(4.33±1.13) vs(5.06±1.06);t=3. 127,3. 157,3. 427, respectively;P<0. 05]. The last time of the experimental group for lateral position were longer than that of control group [(88. 6 ± 15. 2) min vs (79. 3 ± 14. 1) min; t = -3. 239,P =0. 002]. Conclusions It can increase the comfort score, extend the last time for lateral position and lessen incision pain to use the individuation lateral position pillow for patients after cervical spine surgery.

As the medical health service is closely related to health of general public,making harmonious hospital inevitably becomes an important link in constructing a harmonious society.The mutual trust is the most fundamental and active factor in physician-patient relationship.Therefore,construction of a harmonious hospital in the new situation should embark from the hospital connotation construction in great details to reconstruct a harmonious medical relation with mutual trust between doctors and patients.