Centered on the core theme of “making ethics work，” the interview systematically reviewed Professor Eric Meslin’s personal experiences and deep reflections on the American Human Genome Project and the Ethical， Legal， and Social Implications （ELSI） program. As a key practitioner in the field of American bioethics and science policy at the end of the 20th century， Professor Meslin recounted the historical origins， institutionalized operation， and funding mechanisms of ELSI， explained the formation process of interdisciplinary collaboration， policy translation， and governance innovation， and offered insights into the evolution framework of ELSI research over the past three decades. The interview also specifically discussed the expanding trend of bioethics governance in emerging technologies such as genomics， rare disease research， and artificial intelligence， as well as the regulatory challenges of international cooperation， data sharing， and ethical review. This interview provided valuable firsthand materials for understanding the historical experience and future directions of ELSI， offering references for the development of governance systems in medical science and technology ethics in China.

Objective To establish analysis methods of two-dimensional(2-DE)gel electrophoresis for human osteosarcoma.Methods A series of methods,including Immobilized pH gradient were used as ID.Some applications,such as sample preparation used as choice of IPG gel,were improved.Coomassie brilliant blue staining,ImageMaster 2D Elite 3.01 analysis software,MALDI-TOF/ TOF MS and SWISS-PROT database serching were used to separate and indentify the proteins of human osteosarcoma.Results The good use pattern including repetitive experiments showed that in three experiments,the amount of protein spots of the same team sample deviates from the relative standard as following,the average of variation coefficient(%) : 23.00?10.11 and 20.33?9.90;and the range of variation coefficient(%) were:3.80-6.89 and 2.706.89 from osteosarcoma and normal group respectively.The isoelectric point and molecular weigh of the same protein spots in three experiments deviated from relative standard as following:(8.93%?1.17)%,(10.16?2.02)%,(10.87?3.86)%,respectively.Therefore,better resolution and repetitive 2-DE atlas were obtained.The proteins from 11 pairs of sample were analysed by mass spectrometry,9 identified proteins(transthyretin precursor, Triosephosphate isomerase,slow skeletal muscle,cardiac muscle Troponin T,Cofilin-1,Myosin light chain 1,Calgranulin B,Heat-shock protein,Annexin A5, Fanconi anemia group D2 proteins) were more abundant in osteosarcoma tisstues and 2 proteins manganese SOD and carbonic dehydratase appeared down-regulation in osteosarcoma tisstues.Conclusion This optimized 2-DE map is an important tool for further study on osteosarcoma,and these identified proteins were related-proteins with osteosarcoma.It is suggested that the changes of the proteome are involved in the pathology processe of osteosarcoma.