The incidence of papillary thyroid microcarcinoma (PTMC) is steadily rising. Although conventional thyroid surgery techniques are well-established, they present challenges such as functional impairment and overtreatment. Cellular-level fluorescence-guided imaging technology (EndoSCell^®) enables precise intraoperative tumor localization and real-time identification of neurovascular structures via high-resolution imaging and targeted labeling. This technology significantly improves surgical precision, reduces the incidence of postoperative complications including recurrent laryngeal nerve injury and hypoparathyroidism, and strengthens standards for functional preservation. This article aimed to systematically explore recent advances in the application of EndoSCell^® for PTMC management, analyze its influence on the innovation of surgical approaches and the enhancement of functional preservation criteria, and discuss future directions, thereby providing a theoretical basis for clinical practice.

Objective To investigate the feasibility of the spatiotemporal filtering model in analysis of reported schistosomiasis cases, so as to provide insights into analysis of complicated data pertaining to schistosomiasis control. Methods Demographic and epidemiological data of reported schistosomiasis cases in Anhui Province from 1997 to 2010 were collected from Anhui Provincial Center for Disease Control and Prevention, and the annual prevalence of Schistosoma japonicum human infections was calculated. The meteorological data were captured from meteorological stations in counties (cities, districts) of Anhui Province where schistosomiasis cases were reported from 1997 to 2010 at the National Meteorological Information Center, including monthly average air temperature and precipitation. Meteorological data were interpolated using the inverse-distance weighting method, and the annual average air temperature and annual precipitation were calculated in each county (city, district). The centroid of the county (city, district) where schistosomiasis cases were reported was extracted using the software ArcGIS 10.0, and the Euclidean distance from each centroid to the Yangtze River was calculated as the distance between that county (city, district) and the Yangtze River. The global Moran’s I of the prevalence of S. japonicum human infections in Anhui Province for each year from 1997 to 2010 were calculated to analyze the spatial autocorrelation. A spatial weight matrix was constructed using Rook adjacency, and a first-order temporal weight matrix was built to quantify the relationship between disease changes over time. Subsequently, a spatiotemporal structure matrix was constructed. A negative binomial model was built based on the spatiotemporal structure matrix and data pertaining to reported schistosomiasis cases, and a linear model was created between the residual of the model and candidate set feature vectors to determine the optimal subset composition of the spatiotemporal filter through stepwise regression. Then, a spatio-temporal filtering model was constructed using the negative binomial model. Negative binomial models, Bayesian spatial models, and Bayesian spatiotemporal models were constructed and compared with the spatiotemporal filtering model to validate the performance of the spatiotemporal filtering model, and cross-validation was conducted for each model. The goodness of fit was evaluated using the deviance information criterion (DIC) and Watanabe-Akaike information criterion (WAIC), and the effectiveness of model validation was assessed using mean squared error (MSE), while the accuracy of assessment results was assessed using coefficients and their 95% confidence intervals (CI), and the computational efficiency was assessed based on the running time of the model. The four feature vectors with the largest Moran’s I values were selected to identify regions with autocorrelation through their schematic diagrams to investigate the differences in spatiotemporal patterns of specific regions. Results Of all models created, the spatiotemporal filtering model exhibited the highest goodness of fit (DIC = 3 240.70, WAIC = 3 257.80), the best model validation effectiveness (MSE = 42 617.52), and the runtime was 3.18 s, exhibiting the optimal performance. Across all modeling results, the distance from the Yangtze River showed a negative correlation with the number of reported schistosomiasis cases (coefficient values = −4.93 to −3.78, none of the 95% CIs included 0), and annual average air temperature or average precipitation posed no significant effects on numbers of reported schistosomiasis cases (both of the 95% CIs included 0). Schematic diagrams of feature vectors showed that the transmission of schistosomiasis might be associated with water systems in Anhui Province, and localized clustering patterns were primarily concentrated in the northern and western parts of schistosomiasis-endemic areas in the province. Conclusion The spatiotemporal filtering model is an effective spatiotemporal analysis characterized by simple modeling, user-friendly operation, accurate results and good flexibility, which may serve as an efficient alternative to conventional complex spatiotemporal models for data analysis in schistosomiasis researches.

ObjectiveTo analyze the protective effect of different types of influenza vaccines (InfV) against influenza A among the individuals aged between 3‒17 years old, and to provide a scientific basis for the prevention and control of influenza in the future. MethodsA retrospective cohort study was conducted to collect data on the incidence and InfV vaccination of the individuals aged between 3‒17 years during the influenza epidemic season from 2022 to 2023. Vaccine effectiveness (VE) was calculated, and a log-binomial regression model was used to calculate the corrected VE. ResultsThe incidence rate of influenza in InfV vaccinated and un-vaccinated groups was 7.32% (1 937/ 26 446) and 9.65% (4 421/45 837), respectively. After adjusting for age and gender factors, the unadjusted VE (95%CI) was 54.57% (52.24%‒56.78%). The unadjusted VE (95%CI) was 53.66% (50.36%‒56.74%) for males and 55.60% (52.24%‒58.72%) for females, respectively. The unadjusted VE (95%CI) for the age group of 3‒ years, 6‒ years, 9‒ years, 12‒ years, and 15‒17 years were 64.08% (60.89%‒67.01%), 57.40% (53.71%‒60.80%), 57.77% (52.49%‒62.47%), 24.36% (9.49%‒36.79%), and 24.09% (-17.59%‒51.00%), respectively. The unadjusted VE (95%CI) for quadrivalent split-virion inactivated influenza vaccine, trivalent split-virion inactivated influenza vaccine, trivalent subunit influenza vaccine, and trivalent live attenuated influenza vaccine were 53.84% (51.32%‒56.24%), 62.17% (56.28%‒67.26%), 79.83% (69.94%‒86.46%), and 31.59% (19.07%‒42.18%), respectively. ConclusionThe InfV used during the 2022‒2023 influenza season had a good protective effect against influenza A among the individuals aged between 3‒17 years old, especially in those aged between 3‒11 years old.

Esculetin, a natural dihydroxy coumarin derived from the Chinese herbal medicine Cortex Fraxini, has demonstrated significant pharmacological activities, including anticancer properties. Ferroptosis, an iron-dependent form of regulated cell death, has garnered considerable attention due to its lethal effect on tumor cells. However, the exact role of ferroptosis in esculetin-mediated anti-hepatocellular carcinoma (HCC) effects remains poorly understood. This study investigated the impact of esculetin on HCC cells both in vitro and in vivo. The findings indicate that esculetin effectively inhibited the growth of HCC cells. Importantly, esculetin promoted the accumulation of intracellular Fe²⁺, leading to an increase in ROS production through the Fenton reaction. This event subsequently induced lipid peroxidation (LPO) and triggered ferroptosis within the HCC cells. The occurrence of ferroptosis was confirmed by the elevation of malondialdehyde (MDA) levels, the depletion of glutathione peroxidase (GSH-Px) activity, and the disruption of mitochondrial morphology. Notably, the inhibitor of ferroptosis, ferrostatin-1 (Fer-1), attenuated the anti-tumor effect of esculetin in HCC cells. Furthermore, the findings revealed that esculetin inhibited the Nrf2-xCT/GPx4 axis signaling in HCC cells. Overexpression of Nrf2 upregulated the expression of downstream SLC7A11 and GPX4, consequently alleviating esculetin-induced ferroptosis. In conclusion, this study suggests that esculetin exerts an anti-HCC effect by inhibiting the activity of the Nrf2-xCT/GPx4 axis, thereby triggering ferroptosis in HCC cells. These findings may contribute to the potential clinical use of esculetin as a candidate for HCC treatment.
Umbelliferones/administration & dosage* ; Ferroptosis/drug effects* ; Carcinoma, Hepatocellular/physiopathology* ; NF-E2-Related Factor 2/genetics* ; Humans ; Liver Neoplasms/physiopathology* ; Phospholipid Hydroperoxide Glutathione Peroxidase/genetics* ; Animals ; Cell Line, Tumor ; Mice ; Amino Acid Transport System y+/genetics* ; Mice, Inbred BALB C ; Male ; Signal Transduction/drug effects* ; Lipid Peroxidation/drug effects* ; Reactive Oxygen Species/metabolism* ; Mice, Nude

Objective To analyze the levels of serum heparin-binding protein(HBP),serum amyloid A(SAA)and C-reactive protein(CRP)in patients with chronic obstructive pulmonary disease(COPD)compli-cated with pulmonary infection,and to analyze the application effect of the three in prognosis evaluation,in or-der to provide an effective and feasible prognosis evaluation tool for clinical practice.Methods A total of 117 COPD patients complicated with pulmonary infection admitted to the hospital from January 2021 to December 2023 were selected as the observation group,and 117 COPD patients without pulmonary infection in the hospi-tal during the same period were selected as the control group.HBP,SAA and CRP levels were compared in two groups.According to the 28-day survival of the patients in the observation group,the patients were divid-ed into death group and survival group,and the related factors affecting the death of COPD patients complicat-ed with pulmonary infection were analyzed.The receiver operating characteristic(ROC)curve was used to e-valuate the efficacy of single and combined prediction of HBP,SAA,CRP for short-term prognosis in COPD patients complicated with pulmonary infection.Results The levels of HBP,SAA,and CRP in the observation group were higher than those in the control group(P<0.05).Among 117 COPD patients complicated with pulmonary infection,28 cases(23.93%)died after 28 days,and 89 cases(76.07%)survived.The age in the death group was older than that in the survival group(P<0.05),and the proportions of patients with invasive procedures,COPD duration≥10 years,and clinical pulmonary infection score(CPIS)of moderate to severe were higher than those in the survival group(P<0.05),and the levels of serum HBP,SAA,and CRP were higher than those in the survival group(P<0.05).Age,invasive procedures,COPD duration,CPIS,HBP,SAA,and CRP were independent risk factors for death in COPD patients complicated with pulmonary infec-tion(P<0.001).The area under the curve of combined prediction of HBP,SAA and CRP for short-term prognosis in COPD patients complicated with pulmonary infection was greater than that of single detection of the three(P<0.05).Conclusion The levels of serum HBP,SAA,and CRP in COPD patients complicated with pulmonary infection are closely related to the risk of death in prognosis.Analyzing the levels of these three factors could provide strong support for individualized clinical treatment of COPD patients complicated with pulmonary infection.

Objective To use chromosome microarray analysis(CMA)technology to detect and analyze spontaneous abortion tissues,and explore the genetic factors that contribute to the occurrence of spontaneous abortion.Methods The abortion tissues of totally 1 038 patients with spontaneous abortion who came to the hospital for treatment from January 1,2019 to December 31,2021 were collected.CMA technology was used to detect the tissues,and the results were analyzed for genetic etiology.Results Among 1 038 cases of spontane-ous abortion patients,901 cases were qualified for sampling and 901 cases were actually tested.There were 443 cases of chromosomal abnormalities and(or)copy number variations(CNV)with unknown significance de-tected by CMA,with an abnormality rate of 49.17％.There were 41 cases of pathogenic CNV,accounting for 9.26％of the total abnormalities.There were statistically significant differences in the total chromosome ab-normality rate(x2=17.37)and chromosome number abnormality rate(x2=26.43)of aborted fetuses among different age groups of pregnant women(P＜0.001).There were statistically significant differences in the to-tal chromosome abnormality rate(x2=19.63)and chromosome number abnormality rate(x2=22.66)of dif-ferent miscarriage tissues during pregnancy(P＜0.001).Conclusion The application of CMA technology to detect the miscarriage tissue of patients with spontaneous abortion can improve the detection rate of chromo-somal abnormalities in miscarriage embryos,discover potential genetic factors,and provide genetic guidance for patients' next pregnancy.

Lysine-specific demethylase 1 (LSD1) is the firstly discovered histone demethylase. In recent years, LSD1 has become a hot topic in the study of the development and progression of malignancies, and its expression is closely related to the poor prognosis of malignancies. At present, some studies have showed that LSD1 is strongly related to the proliferation, invasion and metastasis of triple-negative breast cancer (TNBC). However, the specific mechanism is not fully understood. This article summarizes the possible mechanism of the development and progression in LSD1 and TNBC, and reviews the latest progress of LSD1 in the clinical research application of TNBC, so as to provide a reference for drug combination therapy in TNBC patients.

【Objective】 To explore the effectiveness and safety of polyglycolic acid (PGA) and hydroxypropyl methylcellulose (HPMC) composite materials in distal pancreatectomy postoperative suturing. 【Methods】 We selected 36 healthy adult beagles and divided them randomly into observation group and control group, with 18 distal pancreatectomy surgeries in each group. The observation group used PGA+HPMC composite materials for incision reinforcement while the control group used NEOVEIL for incision reinforcement. 3 days before surgery, 3 days after surgery, and before dissection, blood routine tests were performed on each group of experimental dogs. Observation periods of 2-week, 4-week and 8-week were set, and six animals at each observation point were evaluated for histological examination of heart, liver, spleen, lung, and kidney tissues, hard tissue slice pathological diagnosis, and safety evaluation. 【Results】 There was no significant difference between the observation group and the control group in the preoperative blood routine test. Repeated measures ANOVA results showed differences in the mean values of white blood cell count (WBC) ( F=14.875, P=0.001), mean corpuscular hemoglobin concentration (MCHC) (F=5.049,P=0.009), neutrophil percentage (Neu%) (F=4.794, P=0.011), red blood cell count (RBC) (F=6.591, P=0.002), hemoglobin (HGB) (F=8.154, P=0.001), hematocrit (HCT) (F=5.281, P=0.007), platelet count (PLT) (F=6.560, P=0.014), red blood cell distribution width coefficient of variation (RDW-CV) (F=33.950, P=0.039), or lymphocyte percentage (Lym%) (F=3.299, P=0.043) at different time points. However, the observation group and the control group did not differ, and the interaction between time and group had no significant effect on the above indicators, suggesting that both groups of dogs had inflammatory response or surgical stress. For the 8-week postoperative hard tissue pathological section score, there was no significant difference between the observation group and the control group in the inflammation and necrosis related score, fibrosis, repair or other related scores and total score (P>0.05). In the dissection at 8 weeks after surgery, no obvious damage to the heart, liver, spleen, lung, kidney, or other organs was found in both groups, nor was there any residual suture material, pancreatic fistula, or pancreatitis, indicating that the suture materials in both groups had been completely absorbed and metabolized, and the incision healed well without causing adverse effects on the visceral organs. 【Conclusion】 PGA and HPMC are effective and safe postoperative suture materials, with good biodegradability, biocompatibility, suture strength, and wound healing quality. They can be comparable to traditional absorbable reinforcement materials in distal pancreatectomy postoperative suturing, thus providing scientific basis for their clinical application.

Objective To investigate the trends in areas of Oncomelania hupensis snail habitats in Anhui Province from 2004 to 2023, so as to provide insights into facilitating the progress of schistosomiasis elimination in the province. Methods Areas of O. hupensis snail habitats in Anhui Province from 2004 to 2023 were collected, and the trends in and mutations of O. hupensis snail habitats in Anhui Province, and the trends in O. hupensis snail habitats in different types of schistosomiasis-endemic foci and in different cities of Anhui Province from 2004 to 2023 were analyzed using Mann-Kendall test. Results The areas of O. hupensis snail habitats reduced by 12.62% in Anhui Province in 2023 relative to in 2004. Mann-Kendall trend test showed that the areas of O. hupensis snail habitats appeared a tendency towards a decline in Anhui Province from 2004 to 2023 (Z = −3.926, P < 0.001, β = −215.66), with an annual average reduction of 215.66 hm². Mann-Kendall mutation test showed that the areas of O. hupensis snail habitats appeared a tendency towards a decline in Anhui Province since 2015 (|Z| > U_0.05, P < 0.05). The areas of O. hupensis snail habitats appeared a tendency towards a decline in marshland and lake regions (Z = −4.899, P < 0.001, β = −260.13), and a tendency towards a rise in hilly regions of Anhui Province from 2004 to 2023 (Z = 2.758, P < 0.01, β = 44.55). Among 9 cities endemic for schistosomiasis in Anhui Province, the areas of O. hupensis snail habitats appeared a tendency towards a decline in 5 cities of Ma’anshan, Tongling, Anqing, Huangshan and Chizhou (Z = −4.056 to −2.109, all P values < 0.05, β = −92.12 to −0.45). The average constituent ratios of areas of O. hupensis snail habitats were 88.19% and 11.81% in marshland and lake regions and hilly regions of Anhui Province from 2004 to 2023, respectively, and the ratio of areas of O. hupensis snail habitats in marshland and lake regions to areas of snail habitats in hilly regions reduced from 9.23∶1 in 2004 to 5.74∶1 in 2023 in Anhui Province, with the ratio appearing a tendency towards a decline (Z = −4.477, P < 0.001, β = −0.16). Conclusions The areas of O. hupensis snail habitats appeared a tendency towards a decline in Anhui Province from 2004 to 2023, with a reduction in areas of O. hupensis snail habitats in marshland and lake regions and a rise in hilly regions. Targeted O. hupensis snail control interventions are required tailored to real snail status.