Objective: To analyze the status of Treponema pallidum (TP) infection among blood donors in Hefei area by evaluating anti-TP reactive donors, and to provide data support for blood screening strategies, evaluation of reagent application, and public health prevention and control strategies. Methods: TPPA confirmation test were performed on 338 anti-TP positive samples of voluntary blood donors at Anhui Blood Center from February to April 2022, July to October 2022, February to June 2023. RPR tests were conducted on samples positive for TPPA. The test results, co-reactivity of TP with HBV, HCV, and HIV, and demographic characteristics of the donors were statistically analyzed. Results: The unqualified rate of anti-TP among blood donors in Hefei area was 0.30% (405/133 587), and the positive rate for TPPA was 67.46% (228/338). Among the TPPA-positive donors, 31.67% were RPR-positive. The co-positive rates of HBV, HCV and HIV in anti-TP reactive blood donors were 0.74% (3/405), 0.49% (2/405), and 1.73% (7/405), respectively, with HIV copositivity being the most common. Most co-positive donors were males aged 31-50 years with a high school education or lower, and all were first-time whole blood donors. Conclusion: The anti-TP unqualified rate among blood donors in Hefei area is at a low-to-mederate level. HIV is the most common co-infection with TP among anti-TP positive donors. The majority of co-infected donors are middle-aged men donating whole blood for the first time.

Objective:This study aimed to investigate the effects of glucagon-like peptide-1 receptor agonists(GLP-1RAs) on the emotional behaviors in wild-type mice and explore the underlying mechanisms.Methods:The study consisted of two parts.(1) Drug effect evaluation: C57BL/6 mice were randomly assigned to saline, semaglutide, or liraglutide groups. After 4 weeks of continuous subcutaneous administration, anxiety-like behaviors were evaluated by the open field tests and elevated plus maze, and depression-like behaviors were assessed by the forced swimming test and tail suspension test. Neurotransmitter levels, including NE, DA, 5-HT, GABA, and glutamate, were measured in target brain regions using LC-MS/MS. Hypothalamic activation subregions were identified by c-fos immunohistochemistry.(2) Time-course analysis: Mice received short-term administration(2 weeks), long-term administration(4 weeks), or drug withdrawal(assessed 4 weeks post-withdrawal). Behavioral performance, hypothalamic neurotransmitter levels, and plasma adrenocorticotropic hormone and corticosterone were measured.Results:Compared with the saline group, both GLP-1RAs significantly prolonged immobility time in the forced swimming and tail suspension tests. Multiple brain regions exhibited neurotransmitter abnormalities, including marked reductions in hypothalamic norepinephrine, dopamine, and γ-aminobutyric acid levels. Both GLP-1RAs significantly increased c-fos positive cell counts in the suprachiasmatic nucleus and arcuate nucleus of the hypothalamus. Time-course analysis revealed that short-term administration did not induce depressive phenotypes, whereas long-term administration led to depression-like behaviors, which recovered after drug withdrawal. Plasma adrenocorticotropic hormone and corticosterone levels were elevated even after short-term treatment and remained high after drug withdrawal, while hypothalamic neurotransmitter abnormalities normalized upon withdrawal.Conclusion:GLP-1RAs induce depression-like behaviors in a time-dependent manner, with neurotransmitter imbalance and persistent hyperactivation of the HPA axis likely contributing to the pathophysiological mechanism.

Objective:This study aimed to investigate the effects of glucagon-like peptide-1 receptor agonists(GLP-1RAs) on the emotional behaviors in wild-type mice and explore the underlying mechanisms.Methods:The study consisted of two parts.(1) Drug effect evaluation: C57BL/6 mice were randomly assigned to saline, semaglutide, or liraglutide groups. After 4 weeks of continuous subcutaneous administration, anxiety-like behaviors were evaluated by the open field tests and elevated plus maze, and depression-like behaviors were assessed by the forced swimming test and tail suspension test. Neurotransmitter levels, including NE, DA, 5-HT, GABA, and glutamate, were measured in target brain regions using LC-MS/MS. Hypothalamic activation subregions were identified by c-fos immunohistochemistry.(2) Time-course analysis: Mice received short-term administration(2 weeks), long-term administration(4 weeks), or drug withdrawal(assessed 4 weeks post-withdrawal). Behavioral performance, hypothalamic neurotransmitter levels, and plasma adrenocorticotropic hormone and corticosterone were measured.Results:Compared with the saline group, both GLP-1RAs significantly prolonged immobility time in the forced swimming and tail suspension tests. Multiple brain regions exhibited neurotransmitter abnormalities, including marked reductions in hypothalamic norepinephrine, dopamine, and γ-aminobutyric acid levels. Both GLP-1RAs significantly increased c-fos positive cell counts in the suprachiasmatic nucleus and arcuate nucleus of the hypothalamus. Time-course analysis revealed that short-term administration did not induce depressive phenotypes, whereas long-term administration led to depression-like behaviors, which recovered after drug withdrawal. Plasma adrenocorticotropic hormone and corticosterone levels were elevated even after short-term treatment and remained high after drug withdrawal, while hypothalamic neurotransmitter abnormalities normalized upon withdrawal.Conclusion:GLP-1RAs induce depression-like behaviors in a time-dependent manner, with neurotransmitter imbalance and persistent hyperactivation of the HPA axis likely contributing to the pathophysiological mechanism.

Schizophrenia is a debilitating mental disorder with complex etiology. Early identification of high-risk individuals and early intervention for early-stage patients can help improve prognosis, but effective identification methods are lacking. In recent years, research on proteomic techniques based on peripheral blood has achieved certain advancements in exploring the etiology of schizophrenia, as well as in early recognition and diagnostic modeling. This paper provides a review of the research methodologies of peripheral blood-based proteomics and the candidate biomarkers identified through these method.

The patient was a 25-year-old male who initially presented with emotional issues and later developed involuntary movements following the use of antipsychotic and antidepressant medications. He was initially misdiagnosed with Tardive Dyskinesia, a condition commonly associated with psychotropic drugs. However, due to the severity of his involuntary movements, the ineffectiveness of treatment, and a notable family history, genetic testing was performed. The test indicated a mutation in the VPS13A gene of the patient, and provided evidence for a final diagnosis of Chorea Acanthocytosis. This case report aims to enhance the recognition of movement disorders among psychiatrists and facilitate earlier identification of neurological diseases whose primary manifestation is involuntary movement.

Objective To systematically evaluate the effect of early rehabilitation activities on diaphragm function in patients with mechanical ventilation.Methods Computerized system search databases included PubMed,Embase,Web of Science,Cochrane Library,China Biology Medicine disc,China National Knowledge Infrastructure,Wanfang Data Knowledge Service Platform,and VIP on the effects of early rehabilitation activities on diaphragm function in patients with mechanical ventilation.The search period was from the creation of the database to March 2025.Two researchers independently performed literature screening,literature quality evaluation,and data extraction.Results 9 papers,including 902 patients,were finally included.Meta-analysis showed that early rehabilitation activities improved diaphragm inspiratory thickness[standard mean difference(SMD)=0.67,95％CI:0.39-0.95,P＜0.000 01],diaphragm expiratory thickness(SMD=0.39,95％CI:0.16-0.63,P=0.0001),diaphragm thickness fraction(SMD=0.76,95％CI:0.58-0.94,P＜0.000 01)and diaphragm excursion(SMD=0.49,95％CI:0.27-0.72,P＜0.000 01),and reduced the incidence of patient failure to withdraw from the machine(relative risk=0.45,95％CI:0.31-0.65,P＜0.0001).Conclusion Early rehabilitation activities can improve diaphragm function in patients with mechanical ventilation,which has good clinical value and is worth standardized promotion and application in intensive care unit;The intervention strategy should be further refined in the future to improve the prevention and treatment strategy of diaphragm dysfunction.

Objective To systematically evaluate the effect of early rehabilitation activities on diaphragm function in patients with mechanical ventilation.Methods Computerized system search databases included PubMed,Embase,Web of Science,Cochrane Library,China Biology Medicine disc,China National Knowledge Infrastructure,Wanfang Data Knowledge Service Platform,and VIP on the effects of early rehabilitation activities on diaphragm function in patients with mechanical ventilation.The search period was from the creation of the database to March 2025.Two researchers independently performed literature screening,literature quality evaluation,and data extraction.Results 9 papers,including 902 patients,were finally included.Meta-analysis showed that early rehabilitation activities improved diaphragm inspiratory thickness[standard mean difference(SMD)=0.67,95％CI:0.39-0.95,P＜0.000 01],diaphragm expiratory thickness(SMD=0.39,95％CI:0.16-0.63,P=0.0001),diaphragm thickness fraction(SMD=0.76,95％CI:0.58-0.94,P＜0.000 01)and diaphragm excursion(SMD=0.49,95％CI:0.27-0.72,P＜0.000 01),and reduced the incidence of patient failure to withdraw from the machine(relative risk=0.45,95％CI:0.31-0.65,P＜0.0001).Conclusion Early rehabilitation activities can improve diaphragm function in patients with mechanical ventilation,which has good clinical value and is worth standardized promotion and application in intensive care unit;The intervention strategy should be further refined in the future to improve the prevention and treatment strategy of diaphragm dysfunction.

Schizophrenia is a debilitating mental disorder with complex etiology. Early identification of high-risk individuals and early intervention for early-stage patients can help improve prognosis, but effective identification methods are lacking. In recent years, research on proteomic techniques based on peripheral blood has achieved certain advancements in exploring the etiology of schizophrenia, as well as in early recognition and diagnostic modeling. This paper provides a review of the research methodologies of peripheral blood-based proteomics and the candidate biomarkers identified through these method.

The patient was a 25-year-old male who initially presented with emotional issues and later developed involuntary movements following the use of antipsychotic and antidepressant medications. He was initially misdiagnosed with Tardive Dyskinesia, a condition commonly associated with psychotropic drugs. However, due to the severity of his involuntary movements, the ineffectiveness of treatment, and a notable family history, genetic testing was performed. The test indicated a mutation in the VPS13A gene of the patient, and provided evidence for a final diagnosis of Chorea Acanthocytosis. This case report aims to enhance the recognition of movement disorders among psychiatrists and facilitate earlier identification of neurological diseases whose primary manifestation is involuntary movement.

Purpose To explore the value of CT-based radiomics in the preoperative prediction of lymphatic invasion of node-negative gastric cancer,and to construct a nomogram combined with clinical variables.Materials and Methods The clinical and CT imaging data of 173 gastric cancer patients with lymph node negative and pathologically confirmed gastric cancer in the Sir Run Run Shaw Hospital from January 2019 to June 2021 were retrospectively analyzed.A total of 60 cases with lymphovascular invasion(LVI)positive patients and 113 cases with LVI negative patients were included,and randomly divided into train cohort(n=121)and test cohort(n=52)at 7∶3.Based on the train cohort,the clinical model,the radiomics model,the fusion model were constructed and verified in the test cohort.Clinical data and conventional CT features included age,gender,tumor marker,tumor location,tumor morphology,enhancement range,etc.The clinical significant variables were selected through univariate and multivariate analysis to establish the clinical model.The tumor regions of interest were segmented and radiomics features were extracted by using the 3D-Slicer software.Key features were screened through least absolute shrinkage and selection operator regression analysis,and then the radiomics model was constructed with random forest algorithm,and converted to random forest score(RF score).The fusion model was constructed via combining clinical significant variables and RF score,and visualized as a nomogram.The receiver operator characteristic curve and area under curve(AUC)were used to evaluate the prediction performance of the models.Decision curve analysis was used to calculate the clinical practicability.Results The radiomics model was superior to the clinical model.The radiomics model AUC of the train cohort and the test cohort were 0.872(0.810 to 0.935)and 0.827(0.707 to 0.947),the clinical model AUC were 0.767(0.682 to 0.852)and 0.761(0.610 to 0.913).The nomogram further improved the predictive efficiency,the AUC in train cohort and test cohort reached 0.898(0.842 to 0.953)and 0.844(0.717 to 0.971),respectively.Decision curve analysis demonstrated clinical benefits of nomogram.Conclusion The radiomics model can be used to preoperatively predict LVI of node-negative gastric cancer.The nomogram can further improve the prediction efficiency.