Aim To investigate the mechanisms of Ke-chuanting granules(KCT)inhibiting the IL-33/ILC2s pathway and pathogenic T cells to intervene in allergic airway inflammation.Methods Network pharmacolo-gy was utilized to analyze the potential targets and mechanisms of KCT-treated asthma.Allergic asthma models were induced in mice using OVA.Lung histo-pathology was conducted to observe injury changes.ELISA and quantitative PCR were utilized to measure key inflammatory factors and their mRNA expression levels in Th2-type asthma.Western blot was used to detect the phosphorylation levels of relevant proteins in the MAPK pathway.Flow cytometry was performed to evaluate the proportions of ILC2s,Th1,Th 17,Th2 and Treg cells.Results Network pharmacology iden-tified 227 main active components and 143 key targets of KCT in treating asthma,primarily enriched in signa-ling pathways such as MAPK and IL-17.Further vali-dation experiments demonstrated that KCT significantly alleviated lung inflammatory injury in asthmatic mice,reduced the number of B cells,production of I L-4,TNF-α and TGF-β,downregulated JNK phosphoryla-tion levels in lung tissue,as well as mRNA levels of Il-33,Bcl11b,Rorα,Tcf-7,Jun,Mapk3 and Mapk14.KCT intervention reduced the numbers of ILC2s and Th 17 cells in lungs and spleens of mice,and inhibited Th2 cell infiltration in lungs.Conclusions KCT ex-hibits therapeutic effects on allergic airway inflamma-tion in asthma,closely associated with the inhibition of the IL-33/ILC2s pathway,pathogenic T cell subsets,and JNK-MAPK signaling pathway.

Objective To investigate the clinical efficacy and safety of single-port thoracoscopic surgery for elderly patients with non-small cell lung cancer(NSCLC).Methods The clinical data of 93 patients with NSCLC who underwent thoracoscopic lobectomy or segmentectomy was collected,the patients were divided into uniportal operation group(40 cases,received single-port thoracoscopic surgery)and single-operation port operation group(53 cases,received single-operation port thoracoscopic surgery)according to the operation methods.The operation time,the amount of blood loss,the number of lymph node dissection,chest drainage volume 3 days after surgery,duration of indwelling drainage tube,postoperative hospital stay,visual analogue scale(VAS)score of postoperative pain,and incidence of postoperative complications of patients between the two groups were compared.The cumulative survival rate between the two groups was compared.Results The operation were successfully completed in both groups.There was no statistically significant difference in terms of operation time,the amount of blood loss,the number of lymph node dissection,chest drainage volume 3 days after surgery,duration of indwelling drainage tube,or postoperative hospital stay of patients between the two groups(P>0.05).There was significant difference in VAS score of postoperative pain of patients between the two groups(P<0.05).There was no early death within 1 months after surgery in both groups.There was no significant difference in the incidence of complications between the two groups(P>0.05).After 4 to 30 months of follow-up,there was no significant difference in the cumulative survival rate between the two groups(P>0.05).Conclusion Single-port thoracoscopic lobectomy or segmentectomy for elderly patients with NSCLC has high safety and feasibility,with less trauma,faster recovery and less postoperative pain.

Objective:To understand the psychological experience of tumor radiotherapy patients after participating in acceptance and commitment therapy (ACT), and to provide reference and supplement for the development of ACT psychological intervention plans for tumor radiotherapy patients.Methods:This study was a qualitative study. Using the purposive sampling method, a total of 17 tumor radiotherapy patients treated at Shanghai Proton and Heavy Ion Center from January to March 2023 were selected as the research objects. Phenomenological research method was used to conduct semi-structured in-depth interviews with patients, and the interview data were analyzed by Colaizzi 7-step analysis method.Results:Three themes were extracted, namely, positive physical and mental experience (including improving physical symptoms, relieving negative emotions and cooperating with particle therapy), gaining personal growth (including learning flexible and varied psychological coping strategies, gaining mutual help and friendship, feeling valuable family affection and drawing a better future life) and recommendations for optimizing the ACT intervention program (including building teams according to the patients' conditions, increasing the frequency of activities, trying to experience relief exercises outdoors and increasing the continuity of the intervention program) .Conclusions:ACT can help cancer radiotherapy patients improve their physical and mental health, cope with psychological problems, so that patients can face the disease, treatment and life more positively.

Objective:To explore the effect of supportive-expressive group psychological intervention on the fear of recurrence in cancer patients receiving radiotherapy.Methods:From September 2022 to February 2023, convenience sampling was used to select 72 tumor inpatients from Shanghai Proton and Heavy Ion Center. According to the random number table method, patients were divided into an intervention group and a control group, with 36 cases in each group. The control group received routine nursing, while the intervention group carried out supportive-expressive group psychological intervention. The fear of recurrence, meaning in life, and perceived social support scores of two groups of patients before and after intervention were compared using the Fear of Progression Questionnaire-Short Form (FoP-Q-SF), Cancer-Meaning in Life Scale (C-MiLS), and Perceived Social Support Scale (PSSS) .Results:A total of 7 cases were lost, with 33 cases in the intervention group and 32 cases in the control group completing the study. Before intervention, there was no statistically significant difference in the general information, fear of recurrence, meaning in life, and perceived social support scores between the two groups of patients ( P>0.05). After supportive-expressive group psychological intervention, the total score of cancer recurrence fear, physical health dimension, and social family dimension scores of the intervention group were (30.42±8.31), (15.58±4.12), and (14.82±4.83), respectively, which were lower than the control group's (36.19±5.64), (18.84±3.48), and (17.09±3.27), and the differences were statistically significant ( t=-3.260, -3.446, and -2.216; P<0.05). After intervention, the total score of the intervention group's meaning in life was (63.42±6.37), which was higher than the control group's (54.91±6.53), and the difference was statistically significant ( t=5.325, P<0.05). After intervention, the perceived social support score of the intervention group was (67.64±7.93), which was higher than the control group's (62.38±10.25), and the difference was statistically significant ( t=2.318, P<0.05). After intervention, the level of fear of recurrence in the intervention group was lower than that before intervention, and the level of meaning in life and perceived social support were higher than those before intervention, with statistical differences ( P<0.05) . Conclusions:Support-expressive group psychological intervention can help cancer patients deal with survival, emotional, and interpersonal issues they face, reduce their fear of recurrence, improve their meaning in life, and perceived social support.

The distribution of Ixodes and Ana plasma carried by Ixodes ticks in Anhui Province was clarified as reference for prevention and control of anaplasmosis.In total,630 hard ticks were collected from Jinzhai County,Hanshan County,Jing-de County and Chaohu City in Anhui Province from April to August 2023.Ticks were identified by morphological analysis and 16S rRNA sequencing.Nested PCR with Anaplasma species-specific primers were used to detect 16S rRNA of Anaplasma spe-cies carried by ticks.A phylogenetic tree was constructed using MEGA11.0 software.Of the identified ticks,18.8％(18/96)were Rhipicephalus microplus and 81.2％(78/96)were Haemaphysalis longicorni in Jinzhai County of Anhui Province,all were H.longicorni in Hanshan County and Chaohu City,while the main species in Jingde County was R.microplus.The posi-tive rate of Anaplasma carried by H.longicornis was 30.9％(102/330),which included A.bovis at 1.8％(6/330),A.phagocytophilum at 21.8％(72/330)and uncultured Anaplasma species at 7.3％(24/330).R.microplus was positively cor-related to A.bovis(13.6％,18/132).The uncultured Anaplasma species was mainly detected in host-free ticks.A.phagocy-tophilum was detected in 24.4％of parasitic ticks and 15.8％of host-free ticks.The positive rates of host-free and parasitic ticks were 19.9％and 17.8％,respectively.These results show that H.longicornis and R.microplus were the dominant ticks in several counties of Anhui Province.H.longicornis and R.microplus as well as free and parasitic ticks all carried Anaplas-ma.

HDAC(histone deacetylase)is a class of epigenetic modifying enzymes that can deacetylate proteins by altering the acetylation status of histones in the nucleus,regulating promoter activation levels,and thereby affecting downstream gene expression.However,expression changes of HDACs during endo-thelial differentiation are still unclear.This study used a three-stage method to induce human induced pluripotent stem cells(hiPSCs)to differentiate into endothelial cells,and qRT-PCR was used to detect the expression changes of class I HDAC(HDAC1,2)and class Ⅱ HDAC(HDAC4,5)genes.It was found that HDAC5 exhibits significant expression changes during endothelial differentiation.It is downreg-ulated by 90％during the mesodermal differentiation stage(P＜0.01),upregulated by 3.7-fold during the vascular precursor stage(P＜0.01),and subsequently downregulated by 70％during the late stage of endothelial differentiation(P＜0.01).Immunoblotting experiments further confirmed that HDAC5 under-goes periodic expression changes during endothelial differentiation.Mechanistic studies have shown that HDAC5 downregulation during the differentiation stage of the mesoderm is mediated by Wnt signaling.CHIR99021 treatment and overexpression of Wnt3a can activate the Wnt signaling pathway,leading to HDAC5 downregulation.Inhibiting the Wnt signaling pathway through IWP-2 promotes the recovery of HDAC5 expression.In addition,it was found that HDAC5 is mainly localized in the nucleus,and IWP-2 restores HDAC5 expression,but it remains in the cytoplasm.Further research suggests that downregu-lation of HDAC5 during mesodermal differentiation may contribute to the expression of the mesodermal marker BraT.Treatment with the HDAC inhibitor BML210 can promote early mesodermal differentiation,interfere with endothelial differentiation of vascular precursor cells,and enhance late-stage endothelial differentiation.In conclusion,HDAC5 displays a stage-specific expression during endothelial differentia-tion,and Wnt signaling activation is the main mechanism regulating the downregulation of HDAC5 during the mesoderm stage.

Extracellular vesicles(EVs)are vesicles with phospholipid bilayer structure actively secreted by cells,which naturally carry various contents derived from mother cells.EVs retain the biological char-acteristics of mother cells and reflect the status of mother cells during their secretion.In order to under-stand the biogenesis of EVs and their biological functions in the body,scientists have made many at-tempts.However,direct detection of EVs has some difficulties and current detection techniques usually use EVs contents as detection objects to achieve the purpose of indirect characterization.The purpose of disease diagnosis can be achieved by analyzing EVs in body fluids,and detailed characterization of EVs can promote the development of precision medicine.EVs belong to endogenous substances and can be used as carriers to deliver drugs,with the advantages of increasing efficacy,reducing drug toxicological effects and passing through biological barriers.When EVs applied as a drug or a carrier for delivering drug,challenges to be addressed include(ⅰ)clear characterization of the contents of EVs,(ⅱ)develop-ment of suitable assays to monitor EVs in vivo to determine and optimize the dose,route of administra-tion,and potential toxicity of EVs,and (ⅲ) determination of the kind and amount of membrane compo-sition of EVs.The diameter of EVs is nanoscale and the content is small,but it is of great value in clini-cal application.The selection of appropriate analytical techniques in the application will enable a compre-hensive characterization of EVs and further advance our understanding of EVs biology to develop novel di-agnostic and therapeutic approaches.In this review,the principles,applications,advantages and disad-vantages of analytical techniques in the study of EVs are introduced,with emphasis on the characteriza-tion techniques of EVs.

Objective:To summarize the clinical characteristics,therapeutic effect and prognostic factors of patients with Hodgkin's lymphoma(HL).Methods:A total of 129 patients with HL diagnosed in Peking University Third Hospital from January 2010 to March 2021 who were given at least one efficacy assessment after treatment were enrolled,and their clinical data,including sex,age,pathological type,Ann Arbor stage,ECOG score,blood test,β2-microglobulin,lactate dehydrogenase level,albumin level were collected.The clinical characteristics,therapeutic effect and long-term prognosis of the patients were summarized and analyzed.Results:In classical HL,nodular sclerosis HL accounted for the highest proportion of 51.6％,followed by mixed cellularity HL(36.5％),lymphocyte-rich classical HL(3.2％),and lymphocyte depletion HL(0.7％),while nodular lymphocyte predominant HL accounted for 4.8％.The 3-year overall survival(OS)rate of HL patients was 89.8％,and 5-year OS was 85.0％.The 3-year progression-free survival(PFS)rate was 73.4％,and 5-year PFS was 63.1％.Multivariate regression analysis indicated that IPI score was an independent negative factor,while hemoglobin(Hb)level was an independent positive factor for OS in HL patients.When the mediastinal mass size was 9.2 cm,it was most significant to judge the survival status of HL patients.5-year OS and 5-year PFS were 97.4％and 76.0％in early-stage HL patients without large mass,respectively,while in patients with advanced-stage HL was 83.4％and 55.9％(both P＜0.05).After 2-4 courses of treatment,the overall response rate(ORR)of patients who received chemotherapy combined with radiotherapy was 95.0％,while that was 89.6％in those with chemotherapy alone.Conclusions:The overall prognosis of patients with HL is satisfactory,especially those in early-stage without large mass.IPI score and Hb level are independent risk factors for the prognosis of HL patients.A 9.2 cm mediastinal mass can be used as the cut-off value for the prognosis of Chinese HL patients.

Objective Analysis of the application effect after using 6S management of delicate instruments in Central Supply Sterilization Department.Methods 6S management's preliminary stage from December 2022 to December 2023,then created a departmental 6S management team and organized a mobilization meeting.Formulated specific implementation steps for 6S management,accepted on-site inspection of 6S management quality by the nursing department and self inspection of Central Supply Sterilization Department quarterly,compared the qualification rate of all instruments packaging and sterille goods supply quarterly,and the timely supply rate of emergency reprocessing instruments,the difference before and after 6S management was statistically significant(P＜0.05).Results The on-site inspection of 6S management quality by the nursing department found that the"seiton"in the first quarter,the"safety"of manual cleaning of employees in the second quarter,the"seiso"in the third quarter,the"safety"of on-site interview in the fourth quarter,and the quality of 6S management in the auxiliary area were quali-fied were unqualified.The qualification rate of all instruments packaging were higher than before using 6S management in the first,second,third,fourth quarters(P＜0.05),and the qualification rate of sterille goods supply were higher than before using 6S management in the first,second,fourth quarters(P＜0.05),and the timely supply rate of emergency reprocessing instru-ments were higher than before using 6S management in the second,third,fourth quarters(P＜0.05).Conclusion 6S manage-ment can improved the key link quality and identified various problems of delicate instrument management in Central Supply Steri-lization Department,among which seiri,seiton,seiso,seiketsu is the focus of daily management.

Objective To develop a GIS-based 3D playback system for the flight human-plane data to realize the fusion of pilots'airborne flight data and physiological data.Methods The 3D playback system was developed with the Browser/Server(B/S)architecture,micro-server model,Java language and Spring Cloud technology framework,which was composed of three functional modules for flight process reproduction,physiological situational awareness and critical event calibration analysis.Results The system developed achieved time synchronization and data fusion of airborne flight data and physiological data with a time synchronization frequency of 1 Hz and a refresh rate of not less than 120 frames/s.Conclusion The system developed with high safety,stability,reliability and accuracy facilitates pilot in-flight physiological monitoring and fusion and simultaneous display of airborne flight data and physiological data,which can be used as an important platform for decision-making support in flight training.[Chinese Medical Equipment Journal,2024,45(10):14-19]