Background/Aims: Liver transplantation (LT) following total hepatectomy is a life-saving treatment for hepatocellular carcinoma (HCC). The HCC recurrence after LT hinders the effectiveness of the procedure. The objective of this study is to develop a pre-operative risk stratification model based on a liquid biopsy. Methods: We conducted a comprehensive multi-omics study of 260 HCC patients from three centers, including clinical data, low-coverage whole-genome sequencing of cell-free DNA (cfDNA) from plasma, as well as whole-exome, single-nucleus RNA, and spatial transcriptomics from matched tumor and non-tumor tissues. Results: We identified cfDNA-derived copy number alteration (CNA) signatures associated with post-transplant recurrence. By integrating cfDNA-derived CNA profiles with single-cell transcriptomic data, we traced recurrence-associated cfDNA to a distinct subpopulation of malignant cells within the primary tumor. These cells were embedded in a pro-metastatic microenvironment of specialized endothelial subtypes and cancer-associated fibroblasts. Notably, most recurrence-associated lesions were detectable in cfDNA prior to liver transplantation (LT). Building on these insights, we developed the ZJU Criteria based on CNA fragments and tumor markers, a pre-LT risk prediction tool that integrates conventional clinical factors with cfDNA-derived CNA signatures, and validated it using internal and independent external cohorts. Conclusion Our findings suggest that post-transplant recurrence commonly originates from advanced subclones that emerge late during tumor evolution. The ZJU Criteria provides an accurate, non-invasive strategy that significantly improves pre-LT risk stratification and clinical decision-making for patients with HCC.

Oligometastatic nasopharyngeal carcinoma (NPC) is a distinct type of metastatic NPC with a relatively favorable prognosis. Due to the heterogeneity of oligometastatic NPC, treatment strategies and prognosis vary significantly among patients. In this paper, we review the current state of research on oligometastatic NPC, including its definition, diagnosis, prognosis, and treatment, with a particular focus on summarizing local therapies for both primary and metastatic lesions, aiming to provide a guidance for the clinical treatment of oligometastatic NPC.

Oligometastatic nasopharyngeal carcinoma (NPC) is a distinct type of metastatic NPC with a relatively favorable prognosis. Due to the heterogeneity of oligometastatic NPC, treatment strategies and prognosis vary significantly among patients. In this paper, we review the current state of research on oligometastatic NPC, including its definition, diagnosis, prognosis, and treatment, with a particular focus on summarizing local therapies for both primary and metastatic lesions, aiming to provide a guidance for the clinical treatment of oligometastatic NPC.

Adenosine 5'-monophosphate-activated protein activated protein kinase (AMPK), a heterotrimeric complex, is an important kinase to regulate glycolipid metabolism and energy balance involved in a variety physiological processes in human body. Many research indicated that the function and activity of AMPK were closely related to inflammation, diabetes and cancers. Recent reports show that inhibition of metformin (a first-line drug) on hepatic glucose in patients with hyperglycemia is associated with AMPK pathway, suggesting that targeting AMPK may be one of the effective strategies for the prevention and treatment of a variety of chronic diseases. Here, we review research progress on the structure, activation and regulation of AMPK in glycolipid metabolism to provide an insight into the basic and clinical research of diabetes therapy.
AMP-Activated Protein Kinases ; Adenosine ; Adenosine Monophosphate ; Energy Metabolism ; Enzyme Activation ; Glycolipids ; Humans

Objective To demonstrate the X-ray and CT features of breast hydatid disease.Methods Of 11 patients with pathologically confirmed breast Echinococcus hydatid disease were collected and the X-ray and CT image data were analyzed.Results Of 11 patients with hydatid cysts,single cyst was found in 9 patients which one cyst was ruptured due to trauma,multiple cyst in 2 patients.Mammography showed small or large shadow in different size,with low or high density and smooth margin.Calcification was found in 5 and 2 patients with egg shell-like calcification along the wall of cyst,3 patients with spotted calcification within cyst.One case had cavity-like change (annular solar eclipse sign).Cystic lesion with a complete capsule was demonstrated on CT scan in 1 patient.Conclusion Molybdenum target mammography can accurately display the imaging characteristics of hydatid cyst and improve the diagnostic ability of breast hydatid cyst in combination with clinical and epidemiological data.

ObjectiveTo investigate the impacts of neural stem cells (NSCs) transplantation on spinal pathology and ultrastructure after spinal cord injury (SCI) in rats and probe into the protective role of tacrolimus (FK506) on neural regeneration.MethodsCompressive SCI at T8 was induced in the adult SD rats,which were randomly assigned to the control group,FK506 group,NSCs group and NSCs + FK506 group.The differences of neural regeneration in each group were compared at days 7,14,28 and 56 after injury by motor evoked potentials ( MEP),HE staining,immunohistochemical staining,ultrastructure observation and image analysis of the myelinated fiber. ResultsThe MEP latency in the NSCs + FK506 group was significantly shorter than that in other groups at day 28 ( P ＜ 0.05 ).HE staining revealed that only local necrosis presented in the NSCs + FK506 group at day 56.More BrdU and NF-200 positive cells were detected with immunohistochemical staining in the other three groups as compared with the control group.Moreover,the positive cells in the NSCs + FK506 group also outnumbered the FK506 group and NSCs group.Electron microscope scan showed edema under the membrane of large myelin sheath in the control group,and classic new myelin sheath and neuraxis in the NSCs + FK506 group at day 56.The regeneration of the nerve fiber in the NSCs + FK506 group was better than that of other three groups (P ＜0.01 ).ConclusionAfter NSCs transplantation for SCI rats,the early combination use of FK506 can improve the pathology and ultrastructure of the regenerative nerve fiber and is conducive to nerve regeneration.

OBJECTIVETo explore the role of hypoxia in skeletal myoblasts proliferation and related mechanism.

METHODSThe numbers and proliferous indexes of skeletal myoblasts were detected by flow cytometer under 20%, 3%, and 10% oxygen concentration. Hypoxia inducing factor 1alpha (HIF-1alpha) mRNA expression was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). HIF-1alpha proteinum in endochylema and intranucelus were respectively detected by Western blot.

RESULTSThe numbers and proliferous indexes were higher (P < 0.05) in hypoxia group than those of control group. The expression HIF-1alpha mRNA had no difference in hypoxia and in normal groups. The level of HIF-1alpha proteinum in endochylema under normoxia was more than that in intranucelus and it was opposite under hypoxia.

CONCLUSIONHypoxia can promote the proliferation of skeletal myoblasts. The possible mechanism of hypoxia promoting the proliferation of skeletal myoblasts might be that low oxygen concentration regulates HIF-la nuclear translocation.

Animals ; Cell Hypoxia ; Cell Proliferation ; Cells, Cultured ; Hypoxia-Inducible Factor 1, alpha Subunit ; genetics ; metabolism ; Male ; Myoblasts, Skeletal ; cytology ; metabolism ; Oxygen ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Wistar

0.05).The effect on dispelling edema,shortening wound-healing time and reducing exudate was better in group A than that in group B(P