Objective:To explore the applied value of three-dimensional(3D)image reconstruction in laparoscopic partial nephrectomy(LPN).Methods:A total of 80 patients who underwent LPN due to renal tumors at Linyi Central Hospital between May 2019 and December 2022 were retrospectively selected.The patients were divided into an observation group and a control group,with 40 cases in each group,according to different preoperative imaging examination methods.After computed tomography urography(CTU)examination,the obtained DICOM data of observation group were used to reconstruct 3D images by 3D image reconstruction system.The planning and intraoperative positioning were conducted through auxiliary surgery plan with 3D model,and patients'conditions were informed to their family members through 3D image model.The size and location of the tumor,and the relationship between the tumor,blood vessels and surrounding tissues and organs were observed.Then,the LPN was performed.The control group used CT images to assist LPN after CTU examination.The differences in variously perioperative clinical indicators between the two groups were compared.Blood creatinine levels of two groups were measured before,after,and during follow-up.Additionally,a questionnaire survey was used to score the understanding level of patients'families for the informed content items.Results:There were no significant differences between the two groups in gender,age,difference of tumor side,maximum tumor diameter,body mass index,the score of R.E.N.A.L score system of renal tumor[maximum diameter?,external convexity rate(E),the closest distance(N),difference of side(A)and longitudinal position(L)],preoperative serum creatinine,and other general data(P>0.05).The occlusion time(21.70±6.39)min of renal artery,the time(9.25±2.95)min of seeking target artery,the operation time(122.55±42.57)min,and the resection time(6.47±2.25)min of tumor in the observation group were all shorter than those in the control group,and the differences of them between two groups were statistically significant(t=-2.87,-4.49,-3.60,-3.41,P<0.05),respectively.The scores of understanding levels of patients'families in the observation group for renal structure,site and size of tumor,the relationship between tumor and their surrounding tissue,and surgical plan and purpose were higher than those in the control group,and the differences of them between the two groups were statistically significant(t=-10.07,-13.86,-2.97,-17.57,-17.12,P<0.05),respectively.There were no significant differences between the observation group and the control group in terms of the volume of intraoperative blood loss,hospital stay duration,positive rate of resection margin,incidence of complication,and levels of postoperative serum creatinine during follow-up(P>0.05).Conclusion:The application of 3D image reconstruction that assists LPN can shorten the time of surgical operation,occlusion time of renal artery,time of resecting tumor and time of seeking target artery,which can improve preoperative communication satisfaction between doctors and patients.

Objective:To explore the applied value of three-dimensional(3D)image reconstruction in laparoscopic partial nephrectomy(LPN).Methods:A total of 80 patients who underwent LPN due to renal tumors at Linyi Central Hospital between May 2019 and December 2022 were retrospectively selected.The patients were divided into an observation group and a control group,with 40 cases in each group,according to different preoperative imaging examination methods.After computed tomography urography(CTU)examination,the obtained DICOM data of observation group were used to reconstruct 3D images by 3D image reconstruction system.The planning and intraoperative positioning were conducted through auxiliary surgery plan with 3D model,and patients'conditions were informed to their family members through 3D image model.The size and location of the tumor,and the relationship between the tumor,blood vessels and surrounding tissues and organs were observed.Then,the LPN was performed.The control group used CT images to assist LPN after CTU examination.The differences in variously perioperative clinical indicators between the two groups were compared.Blood creatinine levels of two groups were measured before,after,and during follow-up.Additionally,a questionnaire survey was used to score the understanding level of patients'families for the informed content items.Results:There were no significant differences between the two groups in gender,age,difference of tumor side,maximum tumor diameter,body mass index,the score of R.E.N.A.L score system of renal tumor[maximum diameter?,external convexity rate(E),the closest distance(N),difference of side(A)and longitudinal position(L)],preoperative serum creatinine,and other general data(P>0.05).The occlusion time(21.70±6.39)min of renal artery,the time(9.25±2.95)min of seeking target artery,the operation time(122.55±42.57)min,and the resection time(6.47±2.25)min of tumor in the observation group were all shorter than those in the control group,and the differences of them between two groups were statistically significant(t=-2.87,-4.49,-3.60,-3.41,P<0.05),respectively.The scores of understanding levels of patients'families in the observation group for renal structure,site and size of tumor,the relationship between tumor and their surrounding tissue,and surgical plan and purpose were higher than those in the control group,and the differences of them between the two groups were statistically significant(t=-10.07,-13.86,-2.97,-17.57,-17.12,P<0.05),respectively.There were no significant differences between the observation group and the control group in terms of the volume of intraoperative blood loss,hospital stay duration,positive rate of resection margin,incidence of complication,and levels of postoperative serum creatinine during follow-up(P>0.05).Conclusion:The application of 3D image reconstruction that assists LPN can shorten the time of surgical operation,occlusion time of renal artery,time of resecting tumor and time of seeking target artery,which can improve preoperative communication satisfaction between doctors and patients.

Objective:To study the effect of psoralen on the proliferation, invasion and migration of HTB-47 and CRL-1932 renal cancer cells cultured in vitro, and to further explore the internal mechanism of psoralen inhibiting renal cancer.Methods:The experimental group was HTB-47 and CRL-1932 renal cancer cells treated with dimethyl sulfoxide solution containing 30 μg/ml psoralen, and the control group was renal cancer cells treated with dimethyl sulfoxide. Scratch test, CCK8, Transwell, and Western blot were used to detect the effect of psoralen on renal cancer cells.Results:Compared with the control group, the proliferation, invasion and migration of renal cancer cells treated with psoralen in the experimental group were significantly inhibited. In the renal cancer cells treated with psoralen, the protein expression levels of MKI67, PCNA, MMP2 and MMP9 were significantly decreased (all P<0.05). Conclusions:Psoralen can significantly inhibit the proliferation, invasion and migration of HTB-47 and CRL-1932 renal cancer cells in vitro. The mechanism may be to inhibit the progression of renal cancer by regulating MKI67, PCNA, MMP2 and MMP9.