Objective:To analyze the epidemiological characteristics of comorbidity of post-traumatic stress disorder and depression symptoms among active firefighters, and explore its influencing factors.Methods:In November 2021, a cross-sectional study was conducted using a combination of convenience sampling and cluster sampling. Active firefighters were selected as the research subjects for a questionnaire survey. 1100 questionnaires were distributed, 1032 were collected, and 1021 were valid, with an effective response rate of 98.93%. The PTSD prevalence and characteristics of firefighters were assessed by using the PTSD Checklist Civilian Version (PCL-C) . The degree of depression was assessed by the Patient Health Questionnaire (PHQ-9) . The coping style was assessed by the Trait Coping Style Questionnaire, and the Social Support Rate Scale was used to investigate the degree of social support. Combined with the general information, the influencing factors of comorbidity of PTSD and symptoms of depression in firefighters were analyzed with binary logistic regression based on single factor analysis.Results:Among 1021 firefighters, 379 had a PHQ-9 score of ≥5, and the prevalence rate of depression was 37.1% (379/1 021) . The prevalence rate of PTSD is 4.6% for 47 individuals with a total PCL-C score of ≥38, and the comorbidity rate of depressive symptoms among PTSD patients is 100%. Logistic regression analysis showed that injuries in the past six months, average/poor self-rated health status, and negative coping were all promoting factors for both the simple depression symptom group and the comorbidity group, while social support was the hindering factor ( P<0.05) . Two sets of OR values show that negative coping and social support have roughly the same degree of impact in both groups. The OR (95% CI) values of the two factors of being injured in the past six months and self-rated as average/poor health status have significantly increased in the comorbidity group. The OR (95% CI) values of being injured in the past six months has increased from 2.20 (1.24, 3.93) in the simple depression symptom group to 3.44 (1.30, 9.09) in the comorbidity group, and that of the self-rated as average and poor health status has increased from 4.91 (3.01, 8.00) to 6.56 (1.97, 21.87) and from 7.96 (2.25, 28.25) to 19.17 (4.75, 77.31) separately ( P<0.05) . Conclusion:The results indicate that firefighters with PTSD generally have comorbidity with depressive symptoms. The common influencing factor of being injured in the past six months is consistent with the pattern of PTSD symptoms triggered by depressive symptoms.

G protein-coupled receptors(GPCRs) are ubiquitously expressed in various human tissues and organs, playing a crucial role in regulating nearly all physiological processes. The dysfunction of GPCRs has been directly linked to the onset and progression of a wide range of diseases, including those related to bone metabolism. This review highlights recent findings on the regulatory mechanisms of GPCRs in osteoporosis(2019 to present) and osteoarthritis(2022 to present), along with a discussion of translational research. Additionally, the article provides an outlook on potential directions for future studies in this area.

Objective:To analyze the epidemiological characteristics of comorbidity of post-traumatic stress disorder and depression symptoms among active firefighters, and explore its influencing factors.Methods:In November 2021, a cross-sectional study was conducted using a combination of convenience sampling and cluster sampling. Active firefighters were selected as the research subjects for a questionnaire survey. 1100 questionnaires were distributed, 1032 were collected, and 1021 were valid, with an effective response rate of 98.93%. The PTSD prevalence and characteristics of firefighters were assessed by using the PTSD Checklist Civilian Version (PCL-C) . The degree of depression was assessed by the Patient Health Questionnaire (PHQ-9) . The coping style was assessed by the Trait Coping Style Questionnaire, and the Social Support Rate Scale was used to investigate the degree of social support. Combined with the general information, the influencing factors of comorbidity of PTSD and symptoms of depression in firefighters were analyzed with binary logistic regression based on single factor analysis.Results:Among 1021 firefighters, 379 had a PHQ-9 score of ≥5, and the prevalence rate of depression was 37.1% (379/1 021) . The prevalence rate of PTSD is 4.6% for 47 individuals with a total PCL-C score of ≥38, and the comorbidity rate of depressive symptoms among PTSD patients is 100%. Logistic regression analysis showed that injuries in the past six months, average/poor self-rated health status, and negative coping were all promoting factors for both the simple depression symptom group and the comorbidity group, while social support was the hindering factor ( P<0.05) . Two sets of OR values show that negative coping and social support have roughly the same degree of impact in both groups. The OR (95% CI) values of the two factors of being injured in the past six months and self-rated as average/poor health status have significantly increased in the comorbidity group. The OR (95% CI) values of being injured in the past six months has increased from 2.20 (1.24, 3.93) in the simple depression symptom group to 3.44 (1.30, 9.09) in the comorbidity group, and that of the self-rated as average and poor health status has increased from 4.91 (3.01, 8.00) to 6.56 (1.97, 21.87) and from 7.96 (2.25, 28.25) to 19.17 (4.75, 77.31) separately ( P<0.05) . Conclusion:The results indicate that firefighters with PTSD generally have comorbidity with depressive symptoms. The common influencing factor of being injured in the past six months is consistent with the pattern of PTSD symptoms triggered by depressive symptoms.

G protein-coupled receptors(GPCRs) are ubiquitously expressed in various human tissues and organs, playing a crucial role in regulating nearly all physiological processes. The dysfunction of GPCRs has been directly linked to the onset and progression of a wide range of diseases, including those related to bone metabolism. This review highlights recent findings on the regulatory mechanisms of GPCRs in osteoporosis(2019 to present) and osteoarthritis(2022 to present), along with a discussion of translational research. Additionally, the article provides an outlook on potential directions for future studies in this area.

ObjectiveTo explore the mediating effect of personality traits between job stress and mental symptoms among firefighters. Methods A total of 974 firefighters in Shandong Province were selected as the research subjects using convenience sampling method. The Job Stress Scale, Brief Version of Chinese Big Five Personality Inventory and Symptom Checklist 90 were used to investigate their job stress, personality traits and mental symptoms. Results Job stress was found at a relatively high level in 39.7% of the firefighters, and 19.2% of the firefighters had high level of neurotic personality trait tendency. The positive detection rate of mental symptoms was 9.9% among firefighters. The score of job stress of the firefighters had a positive correlation with neuroticism and the score of mental symptoms (all P<0.01). The score of job stress and mental symptoms of the firefighters had a negative correlation with the personality traits score of extraversion, openness, agreeableness, and conscientiousness (all P<0.05). Neuroticism played a partially positive mediating effect between job stress and mental symptoms, and its mediating effect accounted for 39.6% of the total effect. Extraversion, agreeableness and conscientiousness played a partially negative mediating effect between work stress and mental symptoms, and the mediating effect accounted for 12.0%, 17.8% and 8.4% of the total effect respectively. There was no mediating effect of openness between job stress and mental symptoms. Conclusion Neurotic personality trait may enhance the negative effects of work stress on firefighters' mental health.

Osteoarthritis (OA) is one of the most common chronic diseases in the world. However, current treatment modalities mainly relieve pain and inhibit cartilage degradation, but do not promote cartilage regeneration. In this study, we show that G protein-coupled receptor class C group 5 member B (GPRC5B), an orphan G-protein-couple receptor, not only inhibits cartilage degradation, but also increases cartilage regeneration and thereby is protective against OA. We observed that Gprc5b deficient chondrocytes had an upregulation of cartilage catabolic gene expression, along with downregulation of anabolic genes in vitro. Furthermore, mice deficient in Gprc5b displayed a more severe OA phenotype in the destabilization of the medial meniscus (DMM) induced OA mouse model, with upregulation of cartilage catabolic factors and downregulation of anabolic factors, consistent with our in vitro findings. Overexpression of Gprc5b by lentiviral vectors alleviated the cartilage degeneration in DMM-induced OA mouse model by inhibiting cartilage degradation and promoting regeneration. We also assessed the molecular mechanisms downstream of Gprc5b that may mediate these observed effects and identify the role of protein kinase B (AKT)-mammalian target of rapamycin (mTOR)-autophagy signaling pathway. Thus, we demonstrate an integral role of GPRC5B in OA pathogenesis, and activation of GPRC5B has the potential in preventing the progression of OA.

{L-End}Objective To analyze the current status of post-traumatic stress disorder (PTSD) among firefighters in Chongqing City and explore its influencing factors. {L-End}Methods A total of 1 021 firefighters in Chongqing City were selected as the study subjects using the convenient sampling method. The PTSD Checklist Civilian Version was used to assess their PTSD symptom and characteristics. The Trait Coping Style Questionnaire and the Social Support Rating Scale were used to investigate coping styles and the level of social support. {L-End}Results The positive detection rate of PTSD among the study subjects was 4.6%(47/1 021), with positive detection rates of re-experiencing, avoidance/numbing, and hyperarousal symptoms of 7.0%, 9.2%, and 16.5%, respectively. The positive detection rates of re-experiencing, avoidance/numbing, and hyperarousal symptoms in the PTSD firefighters were higher than those in non-PTSD firefighters (83.0% vs 3.3%, 93.6% vs 5.1%, 100.0% vs 12.1%, respectively; all P<0.01). The results of the multivariate logistic regression analysis showed that being injured in the past six months and adopting a negative coping style were risk factors for PTSD ［odds ratio (OR) and 95% confidence interval (CI) were 2.65 (1.07-6.56) and 1.26 (1.19-1.33), respectively; both P<0.05］, while adopting a positive coping style and having a higher level of social support were protective factors for PTSD ［OR and 95%CI were 0.90 (0.85-0.95) and 0.95 (0.91-0.99), respectively; both P<0.05］. {L-End}Conclusion The incidence of PTSD among the firefighters in Chongqing City is relatively high, with symptoms mainly characterized by hyperarousal. Being injured in the past six months, coping styles, and the level of social support are influencing factors for PTSD.

Objective:To investigate the current situation of postcholecystectomy syndrome (PCS) and analyze its influencing factors, so as to provide basis for the formulation of postoperative nursing measures.Methods:A total of 336 patients who underwent laparoscopic cholecystectomy in Department of General Surgery in Xuanwu Hospital, Capital Medical University from June 2019 to December 2020 were selected as research objects by the convenient sampling method. General information questionnaire, lifestyle questionnaire, Self-rating Anxiety Scale and Self-rating Depression Scale were used to conduct a questionnaire survey of the patients in 1 month and 3 months after the operation. Logistic regression analysis was used to explore the influencing factors of PCS occurrence.Results:After 3 months of follow-up, 4 patients were lost to follow-up, and data of 332 patients were finally collected. The incidence of PCS was 23.5% (78/332) in 1 month after surgery and 7.2% (24/332) in 3 months after surgery. The total incidence of PCS within 3 months was 30.7% (102/332) , and the main symptoms were diarrhea, abdominal pain and abdominal distension. Logistic regression analysis showed that meat diet and irregular meals were the risk factors for PCS in one month after surgery ( P<0.05) and marriage was the protective factor for PCS in one month after surgery ( P<0.05) . Anxiety was a risk factor for PCS in 3 months after surgery ( P<0.05) . Conclusions:Patients with cholecystectomy have a high incidence of PCS. Patients are advised to reduce the intake of high-fat food, pay attention to the combination of meat and vegetables in their diet and correct irregular eating habits within 1 month after surgery. Meanwhile, family support should be enhanced and emotional management should be strengthened in 3 months after surgery to reduce the incidence of PCS.

Objective:To investigate the risk factors of birth weight discordance in dichorionic diamniotic (DCDA) twins.Methods:This study retrospectively analyzed 1 757 cases of DCDA twin pregnancies from 11 Chinese hospitals from January 1, 2014, to December 31, 2017. Birth weight discordance was defined as ≥ 20% difference between the twins. All cases were divided into two groups: the concordant group ( n=1 520) and discordant group ( n=237). General information was compared and the high-risk factors of birth weight discordance were analyzed. Mann-Whitney U test, Chi-square test or Fisher's exact test, and logistic regression analysis were used as statistical methods. Results:Compared with the concordant group, the discordant group showed a higher incidence of hypertensive disorders of pregnancy [24.5% (58/237) vs 12.8% (194/1 520), χ2=22.882, P<0.05], fetal structural malformations [4.2% (10/237) vs 1.0% (15/1 520), χ2=15.160, P<0.05], fetal distress [6.3% (15/237) vs 1.4% (21/1 520), χ2=22.602, P<0.05], umbilical cord abnormalities [3.8% (9/237) vs 1.2% (18/1 520), χ2=7.607, P<0.05] and abnormal placental cord insertion [3.8% (9/237) vs 1.4% (21/1 520), χ2=34.904, P<0.05], but lower incidence of premature rupture of membranes [11.0% (26/237) vs 16.5% (250/1 520), χ2=4.645, P=0.034]. Logistic regression analysis showed that the independent risk factors of birth weight discordance in DCDA twins were hypertensive disorders of pregnancy ( OR=2.258, 95% CI: 1.620-3.184, P<0.001), fetal structural malformations ( OR=4.268, 95% CI: 1.892-9.631, P<0.001), umbilical cord abnormalities ( OR=2.889, 95% CI: 1.245-6.705, P=0.014) and abnormal placental cord insertion ( OR=2.318, 95% CI: 1.012-5.311, P=0.047). Conclusions:Hypertensive disorders of pregnancy, fetal structural malformations, umbilical cord abnormalities and abnormal placental cord insertion may be the risk factors of birth weight discordance in DCDA twins.

The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549. The 10 µg/ml of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or 10 µg/ml of PA also had no effect on MRC-5 normal cells. PA-L (5 µg/ml) and PA-H (10 µg/ml) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res (5 µg/ml)+PA-H (10 µg/ml) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, NF-κB, Bcl-2, Bcl-xL, procollagen I, collagen I, collagen III and CTGF, TNF-α, IL-1β, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, IκB-α, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.
Carcinoma, Non-Small-Cell Lung ; Caspase 3 ; Caspase 8 ; Caspase 9 ; Cell Line* ; Collagen ; Fibroblasts ; Flow Cytometry ; Humans ; In Vitro Techniques* ; Lung ; Paclitaxel* ; Procollagen ; RNA, Messenger ; Tissue Inhibitor of Metalloproteinase-1 ; Tissue Inhibitor of Metalloproteinase-2 ; Tissue Inhibitor of Metalloproteinase-3 ; Vascular Endothelial Growth Factor A