Objective:To construct a biosafety evaluation index system for major emergency public health events in medical institutions.Methods:Based on previous laboratory biosafety evaluation work, relevant regulations and standards on biosafety in China were collected through literature research and expert consultations. Candidate indicators for constructing the biosafety evaluation system for major emergency public health events in medical institutions were selected, and a framework was established. Two rounds of expert questionnaires were conducted to determine the content of the index system based on experts′ evaluation, and each indicator′s relevance and importance were scored. Finally, two rounds of Delphi consultations were carried out, and the Analytic Hierarchy Process (AHP) was applied to calculate the weights of indicators.Results:The response rates for the total four rounds of questionnaire surveys were all 100%. The first two rounds focused on determining the framework, while the latter two focused on determining the weights for each indicator. The authority coefficients of the expert consultations for the two rounds of weights were 0.65 and 0.70, respectively, indicating the reliability of the research results. In the final round of survey, the Kendall′s coefficients of concordance at each level were all greater than 0.1. Through statistical testing, the P-values were all less than 0.05, indicating good coordination of expert opinions. Ultimately, we established an operational biosafety evaluation system for major emergency public health events in medical institutions, consisting of 4 primary indicators, 26 secondary indicators, and 119 tertiary indicators, with additional deduction items, bonus items, unacceptable items, and monitoring indicators.Conclusions:Based on scientific theory, a biosafety evaluation system for major emergency public health events in medical institutions was constructed, achieving the integration of routine and emergency measures. This system can be used for self-assessment of laboratory biosafety during emergency public health events, addressing the lack of unified standards in biosafety evaluation. Through regular self-assessment, it can enhance the level of biosafety management in medical institution laboratories, to realize the value of application and dissemination.

Objective:To construct a biosafety evaluation index system for major emergency public health events in medical institutions.Methods:Based on previous laboratory biosafety evaluation work, relevant regulations and standards on biosafety in China were collected through literature research and expert consultations. Candidate indicators for constructing the biosafety evaluation system for major emergency public health events in medical institutions were selected, and a framework was established. Two rounds of expert questionnaires were conducted to determine the content of the index system based on experts′ evaluation, and each indicator′s relevance and importance were scored. Finally, two rounds of Delphi consultations were carried out, and the Analytic Hierarchy Process (AHP) was applied to calculate the weights of indicators.Results:The response rates for the total four rounds of questionnaire surveys were all 100%. The first two rounds focused on determining the framework, while the latter two focused on determining the weights for each indicator. The authority coefficients of the expert consultations for the two rounds of weights were 0.65 and 0.70, respectively, indicating the reliability of the research results. In the final round of survey, the Kendall′s coefficients of concordance at each level were all greater than 0.1. Through statistical testing, the P-values were all less than 0.05, indicating good coordination of expert opinions. Ultimately, we established an operational biosafety evaluation system for major emergency public health events in medical institutions, consisting of 4 primary indicators, 26 secondary indicators, and 119 tertiary indicators, with additional deduction items, bonus items, unacceptable items, and monitoring indicators.Conclusions:Based on scientific theory, a biosafety evaluation system for major emergency public health events in medical institutions was constructed, achieving the integration of routine and emergency measures. This system can be used for self-assessment of laboratory biosafety during emergency public health events, addressing the lack of unified standards in biosafety evaluation. Through regular self-assessment, it can enhance the level of biosafety management in medical institution laboratories, to realize the value of application and dissemination.

Objective:This stndy aims to analyze the influence of ChatGPT on medical research and give suggestions on GhatGPT.Methods:This paper expounded the background of ChatGPT, analyze the connection between ChatGPT and medical research management, application and threats of ChatGPT , and gave suggestions to avoid GhatGPT′s damage.Results:Technology will promote the evolution of medical research management. It is recommended to strengthen the supervision of the ChatGPT and research integrity education.Conclusions:It is urgent to establish a scientific management mechanism to take usage the advantages of GhatGPT.

Cerebellar ataxia, neuropathy, vestibular areflexia syndrome (CANVAS) is an autosomal recessive hereditary disorder characterized by cerebellar ataxia, sensory neuropathy and bilateral vestibulopathy. Recently a biallelic intronic AAGGG repeat expansion in the replication factor C1 (RFC1) gene was identified as the cause of this disorder. Clinical studies of genetically-confirmed CANVAS in the past 2 years have significantly expanded the clinical phenotype of the disease and the concept of RFC1-related disease was proposed. The clinical manifestations, characteristic auxiliary examination, genetic changes of CANVAS were reviewed and the new diagnostic criteria to improve clinicians′ awareness of the disease was discussed in this paper.

Anti-kelch-like protein 11 (KLHL11) antibody associated encephalitis is a type of paraneoplastic encephalitis associated with testicular seminoma, with marginal encephalitis symptoms appearing in a few cases. Vertigo, hearing loss, and tinnitus are common and unique clinical symptoms in patients with KLHL11 antibody associated encephalitis. Treatment options include immunotherapy, symptomatic therapy, and antitumor therapy for patients with concomitant tumors. In order to improve the understanding of this disease among clinical medical workers, the authors review the epidemiological characteristics, pathogenesis and clinical manifestations of anti-KLHL11 antibody associated encephalitis as follows.

Retinal vasculopathy with cerebral leukoencephalopathy and systemic manifestations (RVCL-S) is a rare hereditary cerebrovascular disease. The clinical presentations are characterized by early-onset visual impairment, liver and kidney dysfunction and neurological symptoms. Imaging examinations are often misdiagnosed as neoplasms or tumefactive multiple sclerosis for invasive examination. Early and correct identification is essential for optimizing treatment strategies and comprehensive management. This review is to improve the understanding of RVCL-S, which may help to reach more accurate diagnosis and better treatment, and improve further research.

Objective:To analyze the clinical and laboratory features of patients with Coronavirus Disease 2019(COVID-19), for providing reference for the diagnosis and treatment of the disease.Methods:The clinical data of 72 patients confirmed as COVID-19 in Qishan hospital during January 23, 2020 to February 13, 2020 were collected. The data included the general information, clinical manifestations, laboratory tests and CT findings of the lungs. According to the severity of the disease, the patients were divided into the non-severe group and the severe group, and the laboratory tests characteristics of the two groups were compared. SPSS 22.0 statistical software was used for data analysis.Results:In the 72 patients, 40 were males and 32 females, the average age was (46.0±14.8) years. The main clinical manifestations were fever (84.7%) and cough (77.8%). Among them, 45 cases (62.5%) had expectoration with a small amount of white sticky sputum. The main laboratory results showed that lymphocyte count decreased in 26 cases (36.1%), the absolute number of T lymphocytes decreased in 18 cases (25.0%), CD4 + T cell count decreased in 8 cases (11.1%), CD8 + T cell count decreased in 14 cases (19.4%), ESR increased in 42 cases (58.3%), and CRP increased in 31 cases (43.1%). Peripheral blood lymphocyte (L), albumin, CD4 + T cell, CD8 + T cell, T-lymphocyte in severe group (n=61) were lower than those in non-severe group (n=11), while C-reactive protein (CRP), serum amyloid A (SAA), erythrocyte sedimentation rate (ESR), fibrin (FIB), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH) in severe group were higher than those in non-severe group ( P<0.05). In 72 patients, the negative time of the twice nucleic acid detection from nasopharyngeal swabs and sputum specimens were (21.48±7.9) days and (27.79±7.4) days, there were significant differences between the two groups of specimens for two consecutive negative times ( t=-4.532, P=0.000). The main pulmonary imaging manifestations of COVID-19 patients: two lungs in 58 cases, patchy opacities in 51 cases and ground glass shadows in 61 cases. Conclusions:Most of the COVID-19 patients in Yantai and Weihai were common type. The abnormal T lymphocyte, subcohorte and other laboratory indicators may indicate that the patients are more serious.

Objective:To construct a scientific research performance evaluation index system for specialized hospitals based on disciplinary development.Methods:Building a preliminary evaluation index framework of scientific research performance through literature review and expert consultation meetings, combined with the strategic goals of strengthening specialization and weakening generalization; Delphi method was adopted to conduct two rounds questionnaires survey to collect the importance scores for each index, which confirmed the content of the index system; finally, applied the analytic hierarchy process to calculate the weight coefficient of each index.Results:The responding rates of the two rounds questionnaire survey reached 95%, and the authoritative coefficients were 0.75 and 0.76, respectively. The research results were reliable. The coordination coefficients of all levels of the two surveys were greater than 0.1, according to the significance testing, the P values were less than 0.05, and the expert opinions were well coordinated. Finally, we developed a three-level scientific research performance evaluation system under the guidance of disciplinary development, which including 3 first-level indicators, 20 second-level indicators, as well as 56 third-level indicators. Conclusions:A scientific research performance evaluation system was constructed based on scientific theories. This system combined relevant indicators, such as the development of special disciplinary of specialized hospitals with scientific validity and operability. Besides, it could serve as a tool to evaluate the scientific research performance of specialized hospitals taking into account of the practical application value for guiding scientific research development and disciplinary improvement.

Objective: To explore the effect of Wenrun-Xinjin-Peiben paste on the nutrition status, quality of life and pulmonary function in the stable COPD patients. Methods: A total of 94 malnutrition patients with stable COPD were randomly divided into treatment group (47 cases) and control group (47 cases). The patients in the control group took Symbicort 1 suction/time, bid, and the patients were given oxygen therapy, health and diet nutrition education. The patients in the treatment group were given Wenrun-Xinjin-Peiben paste 6 months as well as the control group treatment. The nutritional status (body mass index, triceps skinfold thickness, upper arm circumference, hemoglobin, albumin, prealbumin), quality of life and pulmonary function in the two groups were compared. Results: The curative effect of TCM syndromes was 90.48% in the treatment group, which is significantly higher than 61.90 % in the control group (Z=3.173, P=0.002). The hemoglobin in the treatment group was superior to the control group (135.69 ± 16.42 g/L vs. 122.75 ± 14.99 g/L; t=12.346, P<0.001). The Albumin in the treatment group was superior to the control group (35.89 ± 6.14 g/L vs. 33.01 ± 6.20 g/L; t=2.139, P=0.035). The disease impacts domain of ST George's respiratory questionnaire in the treatment group was superior to the control group (24.9 ± 16.1 vs. 36.6 ± 16.9; t=3.252, P=0.002). Total score of ST George's respiratory questionnaire in the treatment group was superior to the control group (41.4 ± 14.9 vs. 48.0 ± 14.9; t=2.006, P=0.048). The FEV₁ predicted value in the treatment group was superior to the control group (65.22% ± 4.01% vs. 60.17% ± 4.34%; t=5.538, P=0.000). Conclusions The Wenrun-Xinjin-Peiben paste combined with western medicine could help to improve the nutritional status and quality of life of patients in stable COPD.

Objective: To investigate the relationship between blood pressure variability and ambulatory arterial stiffness index (AASI) in both normal subjects and hypertensive patients.
Methods: A total of 280 consecutive subjects without antihypertensive medication were studied. All subjects received ambulatory blood pressure monitoring (ABPM) and AASI was calculated as 1 minus the regression slope of diastolic blood pressure value vs systolic blood pressure value according to ABPM recording.
Results: ① There were 161 subjects with male gender, 138 patients with hypertension, and the average age was (50.4 ± 13.3) years.②Pearson analysis indicated that AASI was related to age (r=0.272, P<0.001), 24-hour mean pulse pressure (r=0.504, P<0.001) , nocturnal diastolic blood pressure dipping (r=-0.334, P<0.001) and standard deviation of 24-hour diastolic blood pressure (r=-0.520, P<0.001).③AASI in anti-dipper hypertensive patients was higher than that in dipper patients, P<0.05;while AASI between dipper and non-dipper patients, dipper and extreme-dipper patients were similar, P>0.05 respectively.④Multiple linear regression analysis demonstrated that with adjusted age, gender, BMI and blood pressure, AASI was independently related to 24-hour mean pulse pressure (β=0.003, P<0.001), nocturnal diastolic blood pressure dipping (β=-0.001, P<0.05), standard deviations of 24-hour systolic blood pressure (β=0.032, P<0.001) and 24-hour diastolic blood pressure (β=-0.064, P<0.001), standard deviation of 24-hour heart rate (β=0.006, P<0.001).
Conclusion:AASI is closely related to blood pressure variability, it’s a comprehensive index for arterial stiffness and blood pressure variability.