Background The positive rate of work-related musculoskeletal disorders (WMSDs) among coal mine workers remains high, which seriously affects the quality of life of the workers. Objective To estimate the prevalence of WMSDs among coal miners in Shanxi Province and analyze their influencing factors. Methods From May to December 2023, 2315 coal miners from a coal mine enterprise inShanxi Province were selected by convenience sampling, and the self-administered Basic Situation Survey Scale, Musculoskeletal Disorders Questionnaire, Effort-Reward Imbalance Questionnaire, and Pittsburgh Sleep Quality Scale were used to evaluate general demographic characteristics, work organization characteristics, living habits, sleep quality, occupational stress, and occurrence of WMSDs symptoms and the corresponding absences (sickness absence) in past 1 year. The positive rate and absenteeism rate of WMSDs were caculated, and Pearson's chi-square test was used for identify influencing factors of WMSDs, and finally a binary logistic regression method was used to further studying the factors. Results A total of 2577 questionnaires were distributed in this survey, and 2315 valid questionnaires were recovered, with an effective recovery rate of 89.9%. The positive rate of WMSDs symptoms was 48.7% (1127/2315), the absenteeism rate due to WMSDs was 22.6% (523/2315), and the waist (17.5%), neck (11.7%), and knee (9.0%) were the most common areas presenting WMSDs symptoms. The results of logistic regression showed that compared with ≤10 years of service, the risk of WMSDs was higher for those with > 20 years of service (OR=2.167, 95%CI: 1.419, 3.311). Compared with the daily working time of ≤8 h, the risk of WMSDs was higher for those >8 h daily working time (OR=1.463, 95%CI: 1.211, 1.767). A higher risk of WMSDs was reported in workers with moderate labor intensity (OR=1.247, 95%CI: 1.009, 1.542) or heavy labor intensity (OR=1.570, 95%CI: 1.215, 2.027) than those with light labor intensity. The higher the education level (OR _{high school and technical secondary school}=1.866, 95%CI: 1.435, 2.425; OR _{junior college/bachelor degree or above}=1.953, 95%CI: 1.445, 2.639), the higher the risk of WMSDs. The risk of WMSDs was higher in workers reporting smoking (OR=1.225, 95%CI: 1.021, 1.470) or alcohol consumption (OR=1.345, 95%CI: 1.122, 1.612). The risk of WMSDs in coal miners with high occupational stress (OR=1.229, 95%CI:1.030, 1.466) or those with sleep disorders (OR=3.616, 95%CI:2.824, 4.629) was higher than that in workers with low occupational stress or no sleep disorders respectively. Conclusion The positive rate of WMSDs among coal mine personnel in Shanxi Province is high, and education level, length of service, daily working hours, smoking, alcohol consumption, labor intensity, sleep disorders, and occupational stress are influencing factors for WMSDs.

Objective: To investigate the impact of milk and egg supplementation on body composition and bone mineral density of rural primary school students in Yunnan Province, so as to provide a reference for developing targeted nutritional intervention strategies. Methods: In December 2023, a cluster sampling method was adopted to select students from grades one to three in four primary schools each from Jinggu and Shidian countys of Yunnan Province, as the intervention group (662 students). Additionally, two boarding primary schools were selected from each county based on the principle of matching scale and student numbers as the control group (455 students). Starting from April 2023, the intervention group received 200 mL milk and 50 g eggs during the break on school days for 8 months, while the control group maintained their usual diet behavior. Body composition was measured by using bioelectrical impedance analysis, and distal radial bone mineral density was assessed via dual energy X-ray absorptiometry in April and December 2023. The intervention effects were analyzed by using a difference in-differences approach. Results: The final measurements of body fat percentage, skeletal muscle mass and fat free mass of the intervention group and the control group of primary school students were significantly higher than the baseline values, and the net effect of milk and egg intervention on these body composition indicators was not statistically significant ( P >0.05, both before and after adjustment). In contrast, bone mineral density increased significantly by 0.02 g/cm 2 in the intervention group. The net intervention effect on bone mineral density was statistically significant ( β=0.02, 95%CI =0.00-0.04), and remained significant after model adjustment ( β=0.02, 95%CI =0.00-0.04) (both P < 0.05). Subgroup analysis showed statistically significant effects of the intervention among girls ( β=0.02, 95%CI =0.00-0.04), day students ( β=0.04, 95%CI =0.01-0.07), and students with normal nutritional status ( β=0.02, 95%CI =0.00-0.04) (all P <0.05). No significant effect of milk and egg supplementation was observed on body composition indicators (all P <0.05). Conclusions Milk and egg supplementation can improve bone mineral density among rural primary school students in Yunnan Province. It is recommended that rural school aged children should increase intake of milk and eggs to support growth and development.

Background China is a major coal producer and consumer country in the world. Coal workers' pneumoconiosis (CWP) is a primary factor endangering the occupational health of coal miners. Research on the disease burden of CWP and its changing trend is significant for disease prevention & control and associated policies. Objective To analyze the disease burden of CWP in China from 1990 to 2021 and its changing trend, and predict the disease burden from 2022 to 2035. Methods Using the Global Burden of Disease Study (GBD) database of 2021, numbers ofincident cases, prevalent cases, deaths, and disability-adjusted life years (DALYs) as well as crude and age-standardized rates of CWP in China were retrieved. Linear regression model was used to calculate the estimated annual percentage change (EAPC) of the age-standardized rates. Joinpoint regression model was used to analyze the temporal trend of disease burden and the disease burden of different sexes and age groups, and Bayesian age-period-cohort (BAPC) model was used to forecast the trend of CWP disease burden. Results In 1990, the incident, prevalent, and deaths cases of CWP in China were 3266, 18872, and 1561, respectively, and the DALYs of CWP were 44614.718 person-years. In 2021, the incident, prevalent, and deaths cases of CWP were 3446, 23975, and 1229, respectively, and the DALYs of CWP were 29610.754 person-years. From 1990 to 2021, the age-standardized incidence, prevalence, mortality, and DALYs rates of CWP in China all showed a downward trend, with the EAPCs of −3.121%, −2.532%, −4.018%, and −4.268%, respectively. The disease burden of CWP in China was mainly concentrated in the male population. The annual average percent change analysis indicated that each standardized rate showed a fluctuating downward trend in different periods. The BAPC model showed that from 2022 to 2035, the age-standardized rates of CWP in China would continue to decline steadily. In 2035, the age-standardized incidence, prevalence, mortality, and DALYs rates of CWP would be reduced to 0.10 per 100000, 0.74 per 100000, 0.03 per 100000, and 0.74 per 100000, respectively. Conclusion The disease burden contributed by CWP in China generally show a downward trend from 1990 to 2021, and it is expected that the age-standardized rates will continue to decline steadily from 2022 to 2035.

Objective To detect the expression of long non-coding RNA plasma-cytoma variant translocation gene 1(lncRNA PVT1)in ovarian cancer tissues and predict its target gene,and then conduct bioinformatics analysis of the target gene.Methods The cancer tissues and incisal margin normal tissues of 125 patients with ovarian cancer who underwent surgical treatment in our hospital from June 2019 to December 2019 were collected.The expression of lncRNA PVT1 in cancer tissues and incisal margin normal tissues was detected by RT-PCR.The expression of lncRNA PVT1 in ovarian cancer tissues of patients with different clinicopathological characteristics was compared.The lncRNA PVT1 target genes were screened out by the online tools Anolnc2 database,GEPIA2 database,and UALCAN database to obtain the intersection.DAVID database was used to analyze the GO function and KEGG pathway enrichment of the screened lncRNA PVT1 target gene.Results Compared with incisal margin normal tissues,the relative expression of lncRNA PVT1 in cancer tissues significantly increased(P<0.05).The relative expression of lncRNA PVT1 in cancer tissues of ovarian patients with TNM stage Ⅲ to Ⅳ,poorly differentiated or undifferentiation were higher than those of ovarian patients with TNM stage Ⅰ to Ⅱ,moderately or well differentiated,respectively(P<0.05).There were 44 target genes of lncRNA PVT1.GO function analysis found that the target gene functions were mainly enriched in cell cycle regulation,transcription regulation of RNA polymerase Ⅱ promoter,transcription factor activity,lysine oxidase activity,extracellular matrix,cytoplasm,etc.KEGG pathway analysis showed that the pathway it participated in were mainly enriched in cancer-related signal pathway,transforming growth factor-β(TGF-β)signal pathway,extracellular matrix receptor interaction,cyclic guanosine phosphate(cGMP)-protein kinase G(PKG)signal pathway,phosphatidylinositol 3-kinases(PI3K)-RAC-alpha serine/threonine-protein kinase(AKT)signal pathway,etc.Conclusion The expression of lncRNA PVT1 is up-regulated in ovarian cancer tissues.Its target genes are enriched in multiple biological processes such as cell components,molecular functions,biological regulation and tumor-related signaling pathways such as TGF-β,CGMP-PKG.lncRNA PVT1 plays a role in ovarian cancer by regulating the expression of target genes.

Objective:To establish an irritant contact dermatitis(ICD)model induced by sodium dodecyl sulfate(SDS)in mice,and explore its endotype to provide an experimental and theoretical basis for subsequent precise treatment.Methods:Mice were randomly divided into two groups(model group and control group),4%SDS was topically applied to induce ICD in mice,saline was used on control group,the dose and frequency were consistent with model group,and the skin lesions of mice were observed.Epidermal thickness and inflammatory cell infiltration were analyzed by HE staining,toluidine blue staining and immunofluorescence staining.Real-time quantitative PCR was performed to investigate mRNA expression levels of cytokines.Results:Compared with control group,mice in ICD model group showed epidermal thickness on the back of neck,and the numbers of inflammatory cells were increased in dermis.The number of neutrophils,macrophages and T cells were increased.Expressions of Il17a and Il17f mRNA levels were increased.Conclusion:SDS-induced ICD model is successfully established,with the elevated infiltration of neutrophils,macrophages and T cells,and secretion of type 17 cytokines.

Objective:To observe the effects of warming needle therapy combined with the modified Hua Tan Tong Luo Tang(decoction for dissipating phlegm and unblocking collaterals)on post-stroke cognitive impairment due to obstruction of wind-phlegm. Methods:A total of 78 patients who met the inclusion criteria were randomly assigned to an observation group and a control group,with 39 patients in each group.Both groups received conventional treatment.The control group was additionally treated with the modified Hua Tan Tong Luo Tang,while the observation group received warming needle therapy in addition to the treatment given to the control group.Scores on the mini-mental state examination(MMSE),Montreal cognitive assessment(MoCA),and activities of daily living(ADL)were assessed before and after treatment.Adverse reactions and clinical efficacy were analyzed and compared between the two groups after treatment. Results:The total effective rate was 82.1%in the observation group and 61.5%in the control group,showing statistical significance between the two groups(P<0.05).After treatment,the MMSE,MoCA,and ADL scores of both groups were significantly higher than those before treatment within each group(P<0.05).Notably,the scores in the observation group were higher than those in the control group(P<0.05).No adverse reactions occurred in either group during the treatment period. Conclusion:Based on the conventional treatment,compared to modified Hua Tan Tong Luo Tang alone,warming needle therapy combined with the modified Hua Tan Tong Luo Tang showed superior clinical efficacy in treating post-stroke cognitive impairment due to obstruction of wind-phlegm,and this combined therapy also showed high safety.

Objective lo investigate the recovery of urinary function and related influencing factors in patients with stress urinary incontinence(SUI)after the tension-free vaginal tape procedure at the mid-urethra.Methods A total of 112 SUI patients who underwent the tension-free vaginal tape procedure at the mid-urethra in Beijing Rehabilitation Hospital Affiliated to Capital Medical University from October 2018 to October 2023 were selected as the research subjects.The postoperative urinary function recovery of all patients was recorded.According to the postoperative urinary function recovery time,patients with a recovery time of≤3 days were included in the rapid recovery group,and those with a recovery time of＞3 days were included in the non-rapid recovery group.Clinical data including age,body mass index,menopausal status,vaginal delivery history,number of deliveries,disease severity,comorbid hypertension,diabetes,history of pelvic organ prolapse,maximum urinary flow rate,postoperative urinary retention,preoperative bladder residual urine volume,maximum bladder volume,hemoglobin(Hb)level,white blood cell(WBC)count,red blood cell(RBC)count,and platelet(PLT)count of patients between the two groups were compared.Factors affecting the recovery of urinary function in SUI patients were analyzed by multivariate logistic regression.Results The recovery time of urinary function in 112 patients with SUI ranged from 1 to 15(4.10±0.78)days.Specifically,64 patients(57.14％)recovered within 3 days,and 48 patients(42.86％)recovered in more than 3 days.The proportions of patients with menopause,history of vaginal delivery,history of pelvic organ prolapse,and postoperative urinary retention in the non-rapid recovery group were significantly higher than those in the rapid recovery group,and the maximum urinary flow rate was significantly lower than that in the rapid recovery group(P＜0.01).There were no significant differences in age,body mass index,number of deliveries,preoperative bladder residual urine volume,maximum bladder volume,Hb level,WBC count,RBC count,PLT count,disease severity,proportion of patients with comorbid hypertension and proportion of patients with diabetes between the two groups(P＞0.05).Multivariate logistic regression analysis showed that menopause,history of vaginal delivery,history of pelvic organ prolapse,postoperative urinary retention and maximum urinary flow rate were influencing factors for the recovery of urinary function in SUI patients after surgery(P＜0.05).Conclusion The recovery of urinary function in SUI patients after a transvaginal tension-free vaginal tape procedure at mid-urethra is not ideal.Menopause,history of vaginal delivery,history of pelvic organ prolapse,postoperative urinary retention and maximum urinary flow rate are the influencing factors for the recovery of urinary function in SUI patients.

Objective To develop a portable medical oxygen purity analyzer capable of real-time detection of multi-compo-nent gases in medical oxygen or aviation oxygen to ensure the safety of oxygen consumption.Methods The portable medical oxygen purity analyzer with STM3F103RC as the main controller involved in a management module,an oxygen detection module,a carbon monoxide/chlorine detection module,a flow/carbon dioxide detection module and a dew point detection module as its hardware components,which had its human-machine interface programmed with DGUS supervision,control and data acquisition(SCADA)software and system program developed with C language under Keil MDK environment.The performance verification of the analyzer developed was carried out in terms of oxygen detection error and stability and errors for measuring carbon monoxide,chlorine and carbon dioxide.Results The analyzer showed high precision when used to detect oxygen with high volume fraction,with long-term stability and the absolute error restrained within±0.1％;the erros for measuring carbon monoxide,chlorine and carbon dioxide were all limited within±5％FS to meet the desired requirements.Condusion The portable medical oxygen purity analyzer developed with high precision,stability and portability can be used for detection of medical oxygen and aviation oxygen.[Chinese Medical Equipment Journal,2024,45(3):36-40]

Objective To investigate the effects of different concentrations of adapalene on the morphology and functions of neuroblastoma cell line SH-SY5Y,as well as its role in inducing cell differentiation and apoptosis.Methods SH-SY5Y cells were divided into control group,low concentration(0.1 pM and 1 pM)adapalene groups,and high concentration(10 μM)adapalene group.Time-lapse microscopy was used to observe the morphological changes of SH-SY5Y cells.Immunofluorescence staining was performed to detect the expression of neuronal specific marker βⅢ-tubulin and mature neuronal marker neurofilament heavy polypeptide(NFH).Multi-electrode array was used to record the electrophysiological features of SH-SY5Y cells.Cell apoptosis was evaluated using a cell apoptosis detection kit.Results Low concentrations of adapalene promoted the formation of neurite outgrowth in SH-SY5Y cells,with the neurites interconnected to form a network.Spontaneous discharge activity was observed in SH-SY5Y cells treated with low concentrations of adapalene.Compared to the control group,the expression of βⅢ-tubulin and NFH increased in the 1 μM adapalene group,while the level of cell apoptosis increased in the high concentration adapalene group(P＜0.05).Conclusions Low concentrations of adapalene can induce differentiation of SH-SY5Y cells into mature functional neurons,while high concentrations of adapalene can induce apoptosis in SH-SY5Y cells.[Chinese Journal of Contemporary Pediatrics,2024,26(3):282-288]

Purpose To prepare multifunctional SiO2@MnO2 nanoplatforms(SiO2@MnO2 NPs)with chemo dynamic therapy(CDT)efficacy and observe their effects on in vitro MRI and ultrasound imaging.Materials and Methods SiO2 nanoparticles(SiO2 NPs)were firstly prepared by dehydration-condensation reaction of ethyl orthosilicate in alcohol phase,and then SiO2@MnO2 NPs were prepared by reduction reaction of polyethylene glyco with KMnO4.The morphology of the two nanoparticles was observed,and the particle size and surface potential were detected.Methylene blue degradation verified the ability of SiO2@MnO2 NPs to produce toxic hydroxyl radicals,one of the reactive oxygen species.Simulation of different conditions to evaluate the effect of nanoparticles for in vitro MRI imaging.To observe the cellular uptake and the intracellular production of reactive oxygen species by SiO2@MnO2 NPs.CDT efficacy of nanoparticles was assessed on human pancreatic cancer cell PANC-1 cells.Results The SiO2@MnO2 NPs was successfully prepared,and the shell of fragmented MnO2 was observed to be encapsulated around the surface of spherical SiO2 NPs under transmission electron microscopy.The average size was(115.9±2.0)nm and the average zeta potential was(-32.51±0.30)mV.Methylene blue was gradually degraded with the increased concentration of SiO2@MnO2 NPs,suggesting the generation of toxic hydroxyl radicals.In vitro simulated tumor microenvironment,SiO2@MnO2 NPs could enhance the MRI and ultrasound imaging effect.Large amount of reactive oxygen species production was detected in PANC-1 cells and exerted CDT effect to kill tumor cells.Conclusion The successfully prepared multifunctional SiO2@MnO2 NPs possess favorable CDT effect and dual-mode imaging effect,which can also kill PANC-1 cells,laying a foundation for synergistic CDT and the construction of multifunctional nanoplatform.