Objective To analyze the mortality characteristics and impact on life expectancy of the major malignant tumors among Juxian residents in 2023.Methods Collect the full cause of death registration data and population data for resi-dents of Juxian in 2023.The mortality,cause eliminated life expectancy,PYLL and other indicators of major malignant tumors were calculated.Results In 2023,the death rate of malignant tumors among registered residence residents in Juxian County ranked second in the total causes of death,with a crude mortality rate of 159.80/100 000 and a standardized mortality rate of 133.75/100 000.The mortality rate of malignant tumors in men was higher than that in women.The mortality rate of malignant tumors increases with age(P＜0.001).The top 5 deaths from malignant tumors are lung cancer,gastric cancer,liver cancer,colorectal cancer,and esophageal cancer.The life expectancy of residents in Juxian County in 2023 is 80.61 years,with an in-crease of 3.71 years after malignant tumor removal;The top 5 malignant tumors with increased life expectancy after removing ma-jor malignant tumors are lung cancer,gastric cancer,liver cancer,colorectal cancer,and esophageal cancer.The potential life loss rate caused by malignant tumors is 22.38 ‰,with an average potential life loss of 15.38 years.Among the main malignant tumors,lung cancer,liver cancer,stomach cancer,colorectal cancer,and leukemia have a higher potential life loss rate,with leukemia having the highest average potential life loss.Conclusion Lung cancer,digestive system cancer,leukemia and breast cancer in women have a greater impact on life expectancy.Targeted measures should be taken to prevent and control lung cancer,liver cancer and other cancers with high life loss rates.

Objective To analyze the mortality characteristics and impact on life expectancy of the major malignant tumors among Juxian residents in 2023.Methods Collect the full cause of death registration data and population data for resi-dents of Juxian in 2023.The mortality,cause eliminated life expectancy,PYLL and other indicators of major malignant tumors were calculated.Results In 2023,the death rate of malignant tumors among registered residence residents in Juxian County ranked second in the total causes of death,with a crude mortality rate of 159.80/100 000 and a standardized mortality rate of 133.75/100 000.The mortality rate of malignant tumors in men was higher than that in women.The mortality rate of malignant tumors increases with age(P＜0.001).The top 5 deaths from malignant tumors are lung cancer,gastric cancer,liver cancer,colorectal cancer,and esophageal cancer.The life expectancy of residents in Juxian County in 2023 is 80.61 years,with an in-crease of 3.71 years after malignant tumor removal;The top 5 malignant tumors with increased life expectancy after removing ma-jor malignant tumors are lung cancer,gastric cancer,liver cancer,colorectal cancer,and esophageal cancer.The potential life loss rate caused by malignant tumors is 22.38 ‰,with an average potential life loss of 15.38 years.Among the main malignant tumors,lung cancer,liver cancer,stomach cancer,colorectal cancer,and leukemia have a higher potential life loss rate,with leukemia having the highest average potential life loss.Conclusion Lung cancer,digestive system cancer,leukemia and breast cancer in women have a greater impact on life expectancy.Targeted measures should be taken to prevent and control lung cancer,liver cancer and other cancers with high life loss rates.

Mannheimia haemolytica is an important pathogen causing respiratory diseases in sheep,and the development of an inactivated vaccine of sheep origin against Mannheimia haemolytica is an effective means of prevention and control of this pathogen.In this study,Mannheimia haemo-lytica isolated from the diseased material collected from a sheep farm was screened for the most suitable vaccine strain by mouse pathogenicity test,and the inactivated vaccine was prepared by combining four antigenic amounts(106,107,108,109 CFU/mL)with three adjuvants(ISA 201 ad-juvant,ISA 206 adjuvant,and aluminum adjuvant),which were used in immunization and attack protection tests,and then screened for immunity and attack protection tests.Immunization and at-tack protection tests were conducted to screen the optimal antigen amount and adjuvants for inacti-vated vaccine preparation.Finally,the vaccine was prepared under the optimal conditions and the dynamic changes of antibody levels in immunized sheep were observed.The results showed that a total of 24 strains of Mannheimia haemolytica of sheep origin were isolated,among which 12 strains of serotype A1 were the dominant serotypes for the infection in this sheep farm;one strain of Mannheimia haemolytica of sheep origin of type A1(Mh1)was screened and obtained as the strain for vaccine preparation.Its LD50 for mice was 3.16 × 107 CFU/mL,with strong pathogenici-ty,and could cause pathological damage such as inflammatory cell infiltration of varying degrees in heart,liver,spleen,lung and kidney tissues;the results of the immunization and attack protection test showed that the optimal antigenic amount of the inactivated vaccine was 109CFU/mL,and the most suitable adjuvant was ISA 201,which stimulated the production of the highest antibody level and provided 100％protection for the mice.The protection rate of mice reached 100％,and good humoral immunity could be induced after immunizing sheep.The inactivated Mannheimia haemo-lytica type A1 vaccine prepared in this study has good immunoprotection effect,and lays a good foundation for the further development of commercial vaccine against Mannheimia haemolytica of sheep origin.

OBJECTIVE To investigate the effect of arctiin （ARC）relieving lipopolysaccharide （LPS）induced inflammatory injury of human nasopharyngeal epithelial cells NP- 69. METHODS The effects of 24 h treatment of 0.000 1，0.001，0.01，0.1， 1.0，10 μmol/L ARC on the proliferation of NP-69 were determined by MTS method. After 0.01，0.1，and 1.0 μmol/L ARC was applied to NP- 69 for 24 h and NP- 69 was pre-treated with 0.01，0.1 and 1.0 μmol/L ARC for 24 h，and then stimulated with 1.0 μg/mL LPS for 24 h，scratch tests were used to detect cell migration in both experiments. LPS stimulated NP- 69 to establish an inflammation injury model. The levels of nitric oxide （NO），tumor necrosis factor α（TNF-α）interleukin-6（IL-6），and IL- 1β in cell supernatants were detected ，and mRNA and protein expression of zonula oecludens protein 1（ZO-1），β-defensin 3（BD3）， Janus kinase 1 （JAK1），signal transducer and activator of transcription 3 （STAT3） in cell supernatant were also detected. RESULTS Compared with normal group ，0.000 1，0.001，0.01，0.1，1.0，10 μmol/L ARC had no effect on the proliferation of NP-69 after 24 h treatment （P＞0.05）. ARC （0.1，1.0 μmol/L）could significantly promote the rate of cell migration （P＜0.05）. For the inflammatory injure of NP- 69 cells stimulated by LPS ，ARC（1.0 μmol/L）could significantly reduce the release of NO ， TNF-α and IL-6（P＜0.05），significantly increased mRNA and protein expression of ZO- 1 and BD 3 but decreased mRNA and protein expression of STAT 3（P＜0.01 or P＜0.05）. CONCLUSIONS ARC has the effect of reducing the inflammatory injury of NP-69 cells induced by LPS ，promoting the physical and immune defense ability of the nasal mucosa epithelial barrierunder inflammatory environment. The mechanism of action may be related to inhibiting IL- 6/JAK1/STAT3 signaling pathway.

OBJECTIVE: To investigate the extracellular release of high mobility group box 1 (HMGB1) in laryngeal Hep-2 carcinoma cells induced by hypoxia and its possible mechanism. METHOD: The changes of HMGB1 concentration in the culture medium as well as HMGB1 protein and mRNA expression in Hep-2 cells were investigated after the cells were cultured with 1% O2 for different durations. Inhibitory effects of MAPK pathway inhibitors (PD98059. SP600125, and SB202190) and nuclear NF-kappaB pathway inhibitor (PDTC) with various concentrations on extracellular HMGB1 release were observed in hypoxia-induced Hep-2 cells. The HMGB1 concentration and HMGB1 protein expression were measured by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. The HMGB1 mRNA expression was determined by real-time quantitative PCR(RT-PCR). RESULT: The HMGB1 concentration in the culture medium and the HMGB1 protein expression in Hep-2 cells increased after the cells were subjected to hypoxia culture for 12 h in a time-dependent manner. The level of HMGB1 mRNA expression in Hep-2 cells increased after the cells were induced by hypoxia for 6h PD98059 and SP600125 with 20 micromol/ L and PDTC with 50 mg/L partly inhibited extracellular release of HMGB1 in hypoxia-cultured Hcp-2 cells. CONCLUSION Hypoxia induces laryngeal carcinoma cells to release HMGH1. which may be related to MAPK/NF-kappaB signaling pathway.
Anthracenes ; Cell Hypoxia ; Cell Line, Tumor ; Flavonoids ; HMGB1 Protein ; metabolism ; Humans ; Imidazoles ; MAP Kinase Signaling System ; NF-kappa B ; antagonists & inhibitors ; metabolism ; Protein Kinase Inhibitors ; Pyridines ; Pyrrolidines ; RNA, Messenger ; genetics ; Thiocarbamates

Chicken embryo fibroblasts(CEFs)are among the most commonly used cells for the study of interactions between chicken hosts and H5N1 avian influenza virus(AIV).In this study,the expression of eleven housekeeping genes typically used for the normalization of quantitative real-time PCR(QPCR)analysis in mammals were compared in CEFs infected with H5N1 AIV to determine the most reliable reference genes in this system.CEFs cultured from 10-day-old SPF chicken embryos were infected with 100 TCID50 of H5N1 AIV and harvested at 3,12,24 and 30 hours post-infection.The expression levels of the eleven reference genes in infected and uninfected CEFs were determined by real-time PCR.Based on expression stability and expression levels,our data suggest that the ribosomal protein L4(RPL4)and tyrosine 3-monooxygenase tryptophan 5-monooxygenase activation protein zeta polypeptide(YWHAZ)are the best reference genes to use in the study of host cell response to H5N1 AIV infection.However,for the study of replication levels of H5N1 AIV in CEFs,the β-actin gene(ACTB)and the ribosomal protein L4(RPL4)gene are the best references.

This is a study on the biodegradable polymers as gene controlled-released coatings for gene transfer. The PELA (poly (Dl-lactic acid)-co-poly (ethylene glycol), and PLGAE (poly (lactic acid)-co-poly (ethylene glycol)-co-poly (glycolic acid) random copolymer) were synthesized and prepared as the coatings of plasmid pCH110 in the transfection. All kinds of factors affecting the loading efficiency, cytotoxicity, transfection efficiency and the course of the degradation and release in vitro were discussed. The average diameters of microspheres of PELA and PLGAE were 1-3 microm and 0.72 microm respectively. The loading efficiency levels of them were 62% and 70% respectively. The transfection efficiency levels of two kinds of pCH110 delivery system for COS-1 cells were higher and two of them had few cytotoxicity. After transfection, the X-gal assay was performed and reported positive for 96 h. The biodegradable polymeric materials as gene carriers possess their potential superiority.
Biocompatible Materials ; DNA ; chemistry ; Drug Carriers ; chemical synthesis ; chemistry ; toxicity ; Gene Transfer Techniques ; Lactates ; chemical synthesis ; chemistry ; toxicity ; Lactic Acid ; chemical synthesis ; chemistry ; toxicity ; Polyethylene Glycols ; chemical synthesis ; chemistry ; toxicity ; Polyglycolic Acid ; chemical synthesis ; chemistry ; toxicity ; Polymers ; chemical synthesis ; chemistry ; toxicity ; Transfection