Mastitis is one of the common and prevalent diseases in dairy cows,and the natural prod-uct daidzein is a kind of natural flavonoids with a wide range of pharmacological and anti-inflam-matory effects.However,the effect of daidzein on mastitis in dairy cows has not been reported.Therefore,in this study,we explored the effects of daidzein on LPS-induced inflammatory response and tight junction damage in dairy mammary epithelial cells Firstly,we pretreated the MAC-T cell line using different concentrations of daidzein,and it was clarified that daidzein below 200 μmol/L had no effect on the cell activity.Next,we examined the production of pro-inflammatory mediators in LPS-stimulated MAC-T cell lines using qRT-PCR,and clarified that daidzein could reduce the production of pro-inflammatory mediators in a concentration-dependent manner.Subsequently,af-ter the expression of Occludin,Claudin3 and ZO-1 was detected by immunofluorescence and West-ern Blot,it was clear that daidzein could alleviate MAC-T cell intercellular tight junction injury.Fi-nally,it was demonstrated that daidzein significantly inhibited LPS-induced activation of the NF-κB signaling pathway within MAC-T using network pharmacological analysis and Western Blot.The above results suggest that daidzein can inhibit LPS-induced inflammatory response and tight junc-tion damage in mammary epithelial cells of cows by suppressing the activation of NF-κB signaling pathway.The present study provides a theoretical basis for the alleviation of mastitis by natural products and further expands the pharmacological effects of daidzein.

[Objective]To explore the disease diagnosis and treatment philosophy embedded in Zhiyi's Mohe Zhiguan(Great Calm and Insight).[Methods]Using a literary research methodology,this study interprets the medical content of Mohe Zhiguan based on classical theories of traditional Chinese medicine(TCM)and traditional Chinese cultural frameworks.A descriptive analysis without subjective interpretation was adopted to examine the diagnostic and therapeutic characteristics in the text,analyze the value and contributions of Zhiyi's medical philosophy,elucidate his unique cognitive model of medicine,and compare it with modern psychosomatic medicine to uncover its contemporary relevance.[Results]Although Mohe Zhiguan primarily serves as a guide to meditative practice,it contains rich insights into medical diagnosis and treatment.These include an emphasis on medical ethics,distinctive perspectives on life,etiology and therapeutic approaches.While some of these concepts may differ from modern understandings of disease pathology and treatment principles,they offer valuable insights for addressing psychosomatic disorders prevalent in contemporary society when viewed through the lens of holistic and mind-body medicine.[Conclusion]Zhiyi,a master of Buddhist classics,integrated TCM and Indian medical traditions to develop a unique medical paradigm.When critically examined as part of China's cultural heritage,his work can complement and enrich research in TCM.Further in-depth exploration and dissemination of his ideas are warranted to advance the study of traditional medical systems.

Mastitis is one of the common and prevalent diseases in dairy cows,and the natural prod-uct daidzein is a kind of natural flavonoids with a wide range of pharmacological and anti-inflam-matory effects.However,the effect of daidzein on mastitis in dairy cows has not been reported.Therefore,in this study,we explored the effects of daidzein on LPS-induced inflammatory response and tight junction damage in dairy mammary epithelial cells Firstly,we pretreated the MAC-T cell line using different concentrations of daidzein,and it was clarified that daidzein below 200 μmol/L had no effect on the cell activity.Next,we examined the production of pro-inflammatory mediators in LPS-stimulated MAC-T cell lines using qRT-PCR,and clarified that daidzein could reduce the production of pro-inflammatory mediators in a concentration-dependent manner.Subsequently,af-ter the expression of Occludin,Claudin3 and ZO-1 was detected by immunofluorescence and West-ern Blot,it was clear that daidzein could alleviate MAC-T cell intercellular tight junction injury.Fi-nally,it was demonstrated that daidzein significantly inhibited LPS-induced activation of the NF-κB signaling pathway within MAC-T using network pharmacological analysis and Western Blot.The above results suggest that daidzein can inhibit LPS-induced inflammatory response and tight junc-tion damage in mammary epithelial cells of cows by suppressing the activation of NF-κB signaling pathway.The present study provides a theoretical basis for the alleviation of mastitis by natural products and further expands the pharmacological effects of daidzein.

[Objective]To explore the disease diagnosis and treatment philosophy embedded in Zhiyi's Mohe Zhiguan(Great Calm and Insight).[Methods]Using a literary research methodology,this study interprets the medical content of Mohe Zhiguan based on classical theories of traditional Chinese medicine(TCM)and traditional Chinese cultural frameworks.A descriptive analysis without subjective interpretation was adopted to examine the diagnostic and therapeutic characteristics in the text,analyze the value and contributions of Zhiyi's medical philosophy,elucidate his unique cognitive model of medicine,and compare it with modern psychosomatic medicine to uncover its contemporary relevance.[Results]Although Mohe Zhiguan primarily serves as a guide to meditative practice,it contains rich insights into medical diagnosis and treatment.These include an emphasis on medical ethics,distinctive perspectives on life,etiology and therapeutic approaches.While some of these concepts may differ from modern understandings of disease pathology and treatment principles,they offer valuable insights for addressing psychosomatic disorders prevalent in contemporary society when viewed through the lens of holistic and mind-body medicine.[Conclusion]Zhiyi,a master of Buddhist classics,integrated TCM and Indian medical traditions to develop a unique medical paradigm.When critically examined as part of China's cultural heritage,his work can complement and enrich research in TCM.Further in-depth exploration and dissemination of his ideas are warranted to advance the study of traditional medical systems.

Ceria nanoparticles(CeO2 NPs)have become popular materials in biomedical and industrial fields due to theirpotential applications in anti-oxidation,cancer therapy,photocatalytic degradation of pollutants,sensors,etc.Many methods,including gas phase,solid phase,liquid phase,and the newly proposed green synthesis method,have been reported for the synthesis of CeO2 NPs.Due to the wide application of CeO2 NPs,concerns about their adverse impacts on human health have been raised.This review covers recent studies on the biomedical applications of CeO2 NPs,including their use in the treatment of various diseases(e.g.,Alzheimer's disease,ischemic stroke,retinal damage,chronic inflammation,and cancer).CeO2 NP toxicity is discussed in terms of the different systems of the human body(e.g.,cytotoxicity,genotoxicity,respiratory toxicity,neurotoxicity,and hepatotoxicity).This comprehensive review covers both fundamental discoveries and exploratory progress in CeO2 NP research that may lead to practical developments in the future.

OBJECTIVE: To analyze the outcome indexes of randomized controlled trials (RCTs) of acupuncture and moxibustion for mild cognitive impairment (MCI) so as to provide a reference for the protocol design and the construction of a core outcome set in relevant clinical trials of acupuncture and moxibustion for MCI. METHODS: From CNKI, Wanfang, VIP, SinoMed, PubMed, Cochrane Library, EMbase, and Web of Science, RCTs of acupuncture and moxibustion for MCI were searched, and the time duration was ranged from January 1st, 2018 to October 31th, 2023. The general information and outcome indexes of the included studies were collected and analyzed through literature screening and data extraction. RESULTS: A total of 46 studies were eligible, with 2 072 samples and 18 diagnostic criteria involved. The outcome indexes were categorized into 7 domains according to functional attributes, including clinical symptoms/signs, TCM symptoms/syndromes, physical and chemical examination, quality of life, psychiatric and mental health, safety assessment, and patient satisfaction. Eighty-four outcome indexes were reported, with the use frequency of 237. Currently, RCTs of acupuncture and moxibustion for MCI were limited in the big difference in neuropsychological assessment tool, the inadequate reports on educational degree/educational years, the diversity of physical and chemical detection indexes, the inconsistency of the time points for outcome evaluation, the insufficient application of primary and secondary outcomes, the lack of the application of TCM diagnosis, pattern/syndrome differentiation and outcomes, and the shortage in long-term prognosis and economic assessment. CONCLUSION It is recommended to specify and develop the core outcome set of acupuncture and moxibustion for MCI to advance the standardization of related research design, the rational selection of outcome indexes, and the reliability of the research results.
Humans ; Acupuncture Therapy ; Cognitive Dysfunction/therapy* ; Moxibustion ; Quality of Life ; Randomized Controlled Trials as Topic ; Treatment Outcome

[Objective]To explore the way that can highlight the characteristics of traditional Chinese medicine(TCM).[Methods]By searching the literature,it analyzes the current situation of traditional Chinese medicine journals,discuss the necessity,feasibility and rally point of setting up local medical culture column in Chinese medicine journals.[Results]Only by highlighting the characteristics of TCM can the connotation of scientific and technological journals of TCM be fully reflected.TCM journals can develop special columns of local medicine culture by exploring local medicine culture,building high-quality editorial teams,establishing core author groups,setting up high-quality review teams and holding journal academic activities.TCM journals should adhere to the characteristics of TCM and take reviving the TCM culture as the first post;learn to keep constant and change,inherit and innovate at the same time;adhere to the principle of letting a hundred flowers bloom,and show the characteristic of local medicine;focus on integrity and innovation,maintain the continuity and stability of the column,and then enhance the reputation and core competitiveness of TCM journals.[Conclusion]TCM culture is the essence of Chinese culture.TCM journals are the main battlefields of TCM academic culture.The establishment of the special column of local medicine culture in TCM journals is not only conducive to the high-quality development of journals,but also conducive to the inheritance of TCM academic culture.

[Objective]To explore the health preservation thoughts contained in Three Essentials of Health Cultivation written by the health practitioner YUAN Huang in Ming Dynasty,providing certain techniques for modern people to cultivate and maintain health.[Methods]By using literature research methods,this paper analyzes the health preservation content of Three Essentials of Health Cultivation and systematically interprets it in conjunction with classic theories of traditional Chinese medicine(TCM)and traditional Chinese culture.By adopting the approach of"passing on the ancient culture without adding anything new to it",the paper delves into the health preservation ideas and methods contained in the book,analyzes the author's contribution to health preservation and the guiding significance of health preservation benefits for people in today's society.[Results]The Three Essentials of Health Cultivation was written by YUAN Huang,who was a native of Jiashan Zhejiang during the Ming Dynasty.This book was extracted by later generations from the YUAN Huang's True Interpretation of Praying for Inheritance.In the book,YUAN Huang elaborated the three major principles of maintaining health:essence,Qi and spirit,namely gathering essence,nourishing Qi and preserving spirit for health preservation.The book contains both the health preservation concepts of TCM and the techniques of Taoism and Buddhism.Its health preservation techniques are friendly and natural which are explained reasonably,making it particularly practical.[Conclusion]The health preservation methods mentioned by YUAN Huang are simple and practical,which are easy to understand and practice for modern people who are obsessed with external factors and have rare leisure time.If one can practice a little while busy,he can achieve the goal of maintaining physical and mental health and promoting a better life.

Objective:To investigate the clinical and genetic characteristics of cerebellar ataxia, neuropathy, vestibular areflexia syndrome (CANVAS) with replication factor C subunit 1 (RFC1) gene mutation to improve the understanding of this disease.Methods:A case of CANVAS diagnosed in the Peking University Third Hospital in January 2021 was reported. Detailed genetic analyses of ataxia were performed with DNA extracted from the peripheral blood of the patient. Studies including pathogenic variants of RFC1 gene causing CANVAS were reviewed and the clinical and genetic characteristics of the disease were summarized.Results:The patient was a 51-year-old female with the prominent manifestation of progressive walking instability. And the clinical data met the diagnostic criteria of CANVAS. The genetic tests excluded other hereditary ataxia mutations and identified the biallelic expansion of the pathogenic variant structure (AAGGG)exp repeat amplification in RFC1 gene. A total of 14 studies on CANVAS with RFC1 gene mutation were reviewed. The overall mutation rate of RFC1 gene in CANVAS was 68%-100%, and it varied in sporadic and familial CANVAS. And the mutation had ethnic differences.Conclusions:Among adult patients with late-onset ataxia, the combination of brain magnetic resonance imaging, electrophysiology tests and vestibular function examination is beneficial to the identification of CANVAS. And the genetic test of RFC1 gene has significant value in the diagnosis of this disease. This patient with CANVAS expands the disease spectrum of ataxia in China, and confirms that RFC1 gene mutation is of great significance in the screening of ataxia disorders in the Chinese population.

Objective The study aims to investigate the effects of different adaptive statistical iterative reconstruction-V（ ASiR-V） and convolution kernel parameters on stability of CT auto-segmentation which is based on deep learning. Method Twenty patients who have received pelvic radiotherapy were selected and different reconstruction parameters were used to establish CT images dataset. Then structures including three soft tissue organs (bladder, bowelbag, small intestine) and five bone organs (left and right femoral head, left and right femur, pelvic) were segmented automatically by deep learning neural network. Performance was evaluated by dice similarity coefficient（ DSC） and Hausdorff distance, using filter back projection(FBP) as the reference. Results Auto-segmentation of deep learning is greatly affected by ASIR-V, but less affected by convolution kernel, especially in soft tissues. Conclusion The stability of auto-segmentation is affected by parameter selection of reconstruction algorithm. In practical application, it is necessary to find a balance between image quality and segmentation quality, or improve segmentation network to enhance the stability of auto-segmentation.
Algorithms ; Humans ; Image Processing, Computer-Assisted ; Neural Networks, Computer ; Radiation Dosage ; Tomography, X-Ray Computed